FastQCFastQC Report
Wed 25 May 2016
SRR1033107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064350
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28600.034.4179951
GGTATCA21650.032.1965331
TCAACGC30350.031.4191254
ATCAACG31000.030.764813
CAACGCA31150.030.4614145
GTACATG76200.030.039081
AACGCAG32100.029.4035996
TATCAAC35200.027.7775062
TACATGG79150.027.6762712
ACATGGG78850.026.5141833
ACGCAGA40000.023.2447667
CATGGGG61000.022.4088694
CGCAGAG41600.022.0120898
GTCGTAC1605.329107E-520.6055091
CGTACAC2151.1996399E-619.6663593
TAGGACC7450.018.915674
GAGTACA52250.018.8393251
GACCGAT1250.00644515318.783657
GCAGAGT48800.018.3794929
AGTACAT51200.018.3625342
Produced by FastQC (version 0.11.5)