FastQCFastQC Report
Wed 25 May 2016
SRR1033107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064350
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44920.21759875990021074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.17516409523578852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.046.2361371
GTATCAA52600.043.3015331
TCAACGC63350.035.5528644
ATCAACG64350.035.073443
CAACGCA64500.034.7731745
TATCAAC66150.034.404222
AACGCAG66150.034.032316
ACGCAGA77700.028.6105297
CGCAGAG81550.027.08438
GTACATG71500.026.8533121
TAATCCG1101.0157308E-425.6474555
TACATGG74900.025.0488222
ACATGGG73150.024.8761023
GCAGAGT87350.024.8549129
GTGGTAT13650.023.098611
CATGGGG57350.021.0710124
GAGTACT54300.020.38094112-13
AGAGTAC83300.019.91420210-11
CAGAGTA88100.019.70932410-11
GAGTACA49350.019.5483631
Produced by FastQC (version 0.11.5)