FastQCFastQC Report
Wed 25 May 2016
SRR1033106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698950
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.033.7334941
GTACGAC1351.333683E-831.3194073
TCAACGC22200.030.472044
GTATCAA23500.029.8667811
ATCAACG23100.028.8789313
CAACGCA24150.028.011565
AACGCAG25100.026.7578986
GTACATG44750.026.1052991
TACATGG45400.025.2643072
TATCAAC27300.024.9677122
ACATGGG46750.023.7156563
ATAACGG1000.00176630123.4833328
CGCAGAG28400.022.4910778
ACGCAGA29200.022.3574187
TGTACGA1903.635796E-722.267032
CATGGGG31000.019.5487964
GTGTACG2201.4574689E-619.270361
GCGTAGT1508.1687793E-418.842131
TAAGCCG1550.001036895318.1849275
CTGTCGC4400.018.146219
Produced by FastQC (version 0.11.5)