##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033106_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1698950 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.289956149386384 31.0 31.0 34.0 30.0 34.0 2 31.4859077665617 33.0 31.0 34.0 30.0 34.0 3 31.6046057859266 34.0 31.0 34.0 30.0 34.0 4 35.008165043114865 37.0 35.0 37.0 33.0 37.0 5 35.10014126372171 37.0 35.0 37.0 33.0 37.0 6 35.275016922216665 37.0 35.0 37.0 33.0 37.0 7 35.23730068571765 37.0 36.0 37.0 33.0 37.0 8 35.2436834515436 37.0 36.0 37.0 33.0 37.0 9 36.90321021807587 39.0 38.0 39.0 34.0 39.0 10-11 36.88272668412843 39.0 38.0 39.0 34.0 39.0 12-13 36.83184555166426 39.0 38.0 39.0 34.0 39.0 14-15 38.26809882574531 41.0 39.0 41.0 34.0 41.0 16-17 38.27576709143883 41.0 39.0 41.0 34.0 41.0 18-19 38.26275140527973 41.0 39.0 41.0 34.0 41.0 20-21 38.29159863445069 41.0 39.0 41.0 34.0 41.0 22-23 38.260236322434444 40.5 39.0 41.0 34.0 41.0 24-25 38.25501574501898 40.5 39.0 41.0 34.0 41.0 26-27 38.211736955178196 40.0 38.5 41.0 33.5 41.0 28-29 38.1866031960917 40.0 38.5 41.0 33.0 41.0 30-31 38.18552870890844 40.0 38.5 41.0 33.0 41.0 32-33 38.25361635127579 41.0 39.0 41.0 33.5 41.0 34-35 38.228153271138055 41.0 39.0 41.0 33.0 41.0 36-37 38.14897907531122 41.0 38.5 41.0 33.0 41.0 38-39 38.06459283675211 41.0 38.0 41.0 33.0 41.0 40-41 38.001983872391776 40.0 38.0 41.0 33.0 41.0 42-43 37.88824097236528 40.0 38.0 41.0 33.0 41.0 44-45 37.759807233879755 40.0 38.0 41.0 32.5 41.0 46-47 37.632191647782456 40.0 38.0 41.0 32.0 41.0 48-49 37.50992083345596 40.0 38.0 41.0 32.0 41.0 50-51 37.38734394773242 40.0 37.5 41.0 31.5 41.0 52-53 37.24277789222755 40.0 37.0 41.0 31.0 41.0 54-55 37.09141822890609 40.0 37.0 41.0 31.0 41.0 56-57 36.93590423496866 40.0 36.0 41.0 31.0 41.0 58-59 36.741606580535034 40.0 36.0 41.0 31.0 41.0 60-61 36.51846611142176 39.0 35.5 41.0 30.5 41.0 62-63 36.253625180258396 39.0 35.0 41.0 30.0 41.0 64-65 35.98501074192884 39.0 35.0 41.0 30.0 41.0 66-67 35.70149945554607 38.0 35.0 40.0 30.0 41.0 68-69 35.39473115747962 37.5 35.0 40.0 29.0 41.0 70-71 35.0494399482033 37.0 34.5 40.0 29.0 41.0 72-73 34.69065305041349 36.5 34.0 39.0 29.0 41.0 74-75 34.32427057888696 36.0 34.0 39.0 29.0 41.0 76-77 33.992545984284405 35.5 34.0 38.5 28.5 40.0 78-79 33.61816680891138 35.0 34.0 37.0 28.0 39.5 80-81 33.25319668030254 35.0 34.0 37.0 27.5 39.0 82-83 33.02401100679832 35.0 34.0 36.5 28.0 39.0 84-85 32.74875629065011 35.0 34.0 36.0 27.0 37.5 86-87 32.48227199152417 35.0 34.0 36.0 27.0 37.0 88-89 32.11848200359046 35.0 33.0 35.5 26.0 37.0 90-91 31.926693251714294 35.0 33.0 35.0 26.0 36.0 92-93 31.74163895347126 35.0 33.0 35.0 25.5 36.0 94-95 31.562695194090466 35.0 33.0 35.0 25.0 36.0 96-97 31.382751699579153 35.0 33.0 35.0 25.0 36.0 98-99 31.183271432355276 35.0 33.0 35.0 24.0 35.5 100 31.014288825451015 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1885034519928901 1101 2 0.1914640053056651 1101 3 0.13892279420972997 1101 4 0.18539404785262548 1101 5 0.2196915820186902 1101 6 0.2813899048790276 1101 7 0.3261015860894716 1101 8 0.24459928514500007 1101 9 0.21974925756431674 1101 10-11 0.29990963152927463 1101 12-13 0.32888680244621327 1101 14-15 0.401771498200965 1101 16-17 0.30742562887439817 1101 18-19 0.4020445773892263 1101 20-21 0.34755128080040265 1101 22-23 0.3895697107319904 1101 24-25 0.3987543732445644 1101 26-27 0.41837396331071375 1101 28-29 0.482182015499518 1101 30-31 0.5121540204283761 1101 32-33 0.5728428922611926 1101 34-35 0.5350349124723266 1101 36-37 0.49228833659215354 1101 38-39 0.5906645184811694 1101 40-41 0.5143237783493149 1101 42-43 0.5323265996537359 1101 44-45 0.4109927500566428 1101 46-47 0.5764982912332712 1101 48-49 0.4835522539838166 1101 50-51 0.4733596669083653 1101 52-53 0.5182942836277675 1101 54-55 0.5304831277435369 1101 56-57 0.6111969833853905 1101 58-59 0.5988913921676513 1101 60-61 0.5261963311131836 1101 62-63 0.6533874501596273 1101 64-65 0.6190978068632731 1101 66-67 0.5660497314778254 1101 68-69 0.5198391818087202 1101 70-71 0.5332343497206296 1101 72-73 0.5407745271683169 1101 74-75 0.48245563339923336 1101 76-77 0.5108895196692913 1101 78-79 0.457012812093474 1101 80-81 0.47606561926104973 1101 82-83 0.550279635721374 1101 84-85 0.41770567296419614 1101 86-87 0.5366042916322726 1101 88-89 0.6331862291740862 1101 90-91 0.6743416316399653 1101 92-93 0.6485642662777593 1101 94-95 0.6712636956672036 1101 96-97 0.694675170482359 1101 98-99 0.7347534492331249 1101 100 0.7665773186950027 1102 1 -0.01951520763867265 1102 2 0.026541017802031774 1102 3 0.036334449685902825 1102 4 0.18813222801016138 1102 5 -0.03671983568305137 1102 6 0.09082502076575594 1102 7 0.1753752418147414 1102 8 0.13507207884359929 1102 9 0.21375017092007198 1102 10-11 0.1980298925527677 1102 12-13 0.31311160118713843 1102 14-15 0.2853761963217991 1102 16-17 0.27454010405338636 1102 18-19 0.2598252738795992 1102 20-21 0.2322335122568262 1102 22-23 0.18601309588505188 1102 24-25 0.15075941557218897 1102 26-27 0.15651826597115104 1102 28-29 0.19823782832952475 1102 30-31 0.21311776171469887 1102 32-33 0.24816568048721166 1102 34-35 0.2199281215590787 1102 36-37 0.23760361416549358 1102 38-39 0.17545940972767227 1102 40-41 0.20939222341776542 1102 42-43 0.2731062355946605 1102 44-45 0.23777124618997192 1102 46-47 0.1792357165513394 1102 48-49 0.1712629013718825 1102 50-51 0.15488063068740132 1102 52-53 0.10905240664395421 1102 54-55 0.21240226934009598 1102 56-57 0.07422588996268331 1102 58-59 0.09939524751020912 1102 60-61 0.11175738441616545 1102 62-63 0.2558512461718081 1102 64-65 0.21351218353570545 1102 66-67 0.06630354624776658 1102 68-69 0.07557747141797932 1102 70-71 0.08912172012413322 1102 72-73 0.01761133465029019 1102 74-75 0.0147825770164971 1102 76-77 0.005311256026516276 1102 78-79 -0.06556349274089968 1102 80-81 -0.06064411099706035 1102 82-83 -0.10119966091920674 1102 84-85 0.007200383426649637 1102 86-87 0.039760464143284935 1102 88-89 0.15352726564415065 1102 90-91 0.11347043367199561 1102 92-93 0.21189341025206687 1102 94-95 0.1415145288212578 1102 96-97 0.029749352330735945 1102 98-99 0.04062194865001345 1102 100 -0.07624057250998462 1103 1 0.09204003824493157 1103 2 0.0022215830348351062 1103 3 0.04596076528236637 1103 4 0.13540446632717362 1103 5 0.045278037359594236 1103 6 0.14203701973894312 1103 7 0.25040549594947237 1103 8 0.1603144175187694 1103 9 0.1484317996315525 1103 10-11 0.18519174911760672 1103 12-13 0.1815427867825079 1103 14-15 0.15995600000607624 1103 16-17 0.14658956192374006 1103 18-19 0.165163447874491 1103 20-21 0.14065516321578286 1103 22-23 0.2132754328488815 1103 24-25 0.20214254904487206 1103 26-27 0.1817972763247866 1103 28-29 0.2087320403162778 1103 30-31 0.2019542915960102 1103 32-33 0.1151086071316243 1103 34-35 0.2908847634112419 1103 36-37 0.2758888464805693 1103 38-39 0.25459689185023393 1103 40-41 0.2990819810179701 1103 42-43 0.21102413993047975 1103 44-45 0.29196281903826105 1103 46-47 0.3318021228490906 1103 48-49 0.290118867242775 1103 50-51 0.25142799216013145 1103 52-53 0.20276076900903206 1103 54-55 0.19931353610211744 1103 56-57 0.14568982336691505 1103 58-59 0.16743954689778917 1103 60-61 0.12504475591249786 1103 62-63 0.23166627422564545 1103 64-65 0.18231986502996023 1103 66-67 0.15288843507146055 1103 68-69 0.13052081367475665 1103 70-71 0.227707049007968 1103 72-73 0.39904263026068776 1103 74-75 0.3641139300791991 1103 76-77 0.263797693617434 1103 78-79 0.2404952797357751 1103 80-81 0.24271012519299973 1103 82-83 0.2671490133996173 1103 84-85 0.2784760688814458 1103 86-87 0.27345990306660184 1103 88-89 0.39930085470133037 1103 90-91 0.4838941137971666 1103 92-93 0.28858611381044597 1103 94-95 0.27325447772538425 1103 96-97 0.23658067483370004 1103 98-99 0.21098023094859286 1103 100 0.3129498390684873 1104 1 0.010822591567052342 1104 2 0.03276271977845013 1104 3 0.0703210520687314 1104 4 0.05713362649269271 1104 5 0.0023845560156772194 1104 6 0.08700430468088882 1104 7 0.08688935026280831 1104 8 0.11236605377908404 1104 9 0.19072543091419902 1104 10-11 0.23299581902811894 1104 12-13 0.19499874132899464 1104 14-15 0.1334341599458 1104 16-17 0.20735692602114852 1104 18-19 0.2137975244320387 1104 20-21 0.15845293493850932 1104 22-23 0.13628294120206874 1104 24-25 0.1898937471593527 1104 26-27 0.22495698771176365 1104 28-29 0.2372277723902272 1104 30-31 0.1941672610425016 1104 32-33 0.208772030834119 1104 34-35 0.2592305028425983 1104 36-37 0.29770504278106813 1104 38-39 0.2703625783336747 1104 40-41 0.2823595947341957 1104 42-43 0.3167281701364075 1104 44-45 0.2981812488169595 1104 46-47 0.34064503538990465 1104 48-49 0.2411218362072347 1104 50-51 0.257493803465664 1104 52-53 0.1722366779650173 1104 54-55 0.093520630411291 1104 56-57 0.09257566126056815 1104 58-59 0.15394910454048016 1104 60-61 0.26237482217983654 1104 62-63 0.20941592792045327 1104 64-65 0.14233541070799305 1104 66-67 0.0780766830209032 1104 68-69 0.09599502662327097 1104 70-71 0.07103368139929955 1104 72-73 0.20378818474222982 1104 74-75 0.13335226162391933 1104 76-77 0.0760326528092321 1104 78-79 0.13224065789344053 1104 80-81 0.0829746363952708 1104 82-83 0.11785215247222425 1104 84-85 0.14238574031011808 1104 86-87 0.14782897433443054 1104 88-89 0.19359311408264546 1104 90-91 0.19832925261099987 1104 92-93 0.09577348458025625 1104 94-95 0.1259081205261694 1104 96-97 0.2804022350590891 1104 98-99 0.18205653289119894 1104 100 0.24263276051429017 1105 1 -0.10690103168207443 1105 2 -0.11882779897309703 1105 3 -0.08697749773979524 1105 4 -0.0752314118630224 1105 5 -0.08199204418081507 1105 6 -0.04180094727119865 1105 7 -0.03851414426007693 1105 8 -0.06700448864005892 1105 9 -0.11333824128715975 1105 10-11 -0.027301177441337643 1105 12-13 -0.04326071652136676 1105 14-15 -0.0647810314162669 1105 16-17 -0.01532580569954689 1105 18-19 0.015130832652246795 1105 20-21 -0.014414072041716963 1105 22-23 0.07251870940663707 1105 24-25 0.036048252945171555 1105 26-27 0.09024923106167648 1105 28-29 0.14092404253892 1105 30-31 0.13308132941881468 1105 32-33 0.0950469847117077 1105 34-35 0.11067438540624153 1105 36-37 0.10535476298403523 1105 38-39 0.06436882415325584 1105 40-41 0.07995954499794067 1105 42-43 0.060283512740781475 1105 44-45 0.06004368153214301 1105 46-47 0.08671015986528374 1105 48-49 0.07918983705495464 1105 50-51 0.15573991154278133 1105 52-53 0.07836539991487257 1105 54-55 0.01592335071470785 1105 56-57 0.08383208042201318 1105 58-59 0.054987823476764675 1105 60-61 -0.04359354323705844 1105 62-63 0.06877496423771845 1105 64-65 0.042129881163923244 1105 66-67 -0.023095242703028873 1105 68-69 0.0017092287729525424 1105 70-71 -0.12100758437603076 1105 72-73 -0.1589748293725748 1105 74-75 -0.050641853328073694 1105 76-77 -0.09196830210228768 1105 78-79 -0.08628952564067305 1105 80-81 -0.14957541223845539 1105 82-83 -0.0846797412049014 1105 84-85 -0.13140864561868426 1105 86-87 -0.011507567354591686 1105 88-89 0.07396326632947847 1105 90-91 0.09633020139736104 1105 92-93 0.06299893373780208 1105 94-95 0.07784694616263366 1105 96-97 0.22435401294630353 1105 98-99 0.15020167392899708 1105 100 0.26646022227495436 1106 1 0.07886365988261446 1106 2 0.1013419429092366 1106 3 0.12213199719861123 1106 4 0.07540861758364059 1106 5 -0.013186417815788332 1106 6 0.1109118624452421 1106 7 0.04883867461967384 1106 8 0.07332876097470375 1106 9 0.1315813739733258 1106 10-11 0.0685973484954161 1106 12-13 -0.009169610833730246 1106 14-15 -0.00937912561575871 1106 16-17 0.025169248442097114 1106 18-19 0.01837900371970136 1106 20-21 0.004440129984111252 1106 22-23 0.016594059697261798 1106 24-25 0.0220953902772294 1106 26-27 0.058067594779288356 1106 28-29 0.08497239806563073 1106 30-31 0.09825681626416838 1106 32-33 0.07370329871242376 1106 34-35 0.05612341123829623 1106 36-37 0.14176264581303855 1106 38-39 0.21485046443266498 1106 40-41 0.1376904576118605 1106 42-43 0.12333224470275184 1106 44-45 0.10625200594179063 1106 46-47 0.09138815856595528 1106 48-49 0.10855305592850328 1106 50-51 0.027039839178911507 1106 52-53 0.1358778189976988 1106 54-55 0.14391395295745468 1106 56-57 0.12979111340692384 1106 58-59 0.08553933396057545 1106 60-61 0.07517670656443443 1106 62-63 0.11883526248660559 1106 64-65 0.16911854515279146 1106 66-67 0.052489648819786794 1106 68-69 0.07911388058226265 1106 70-71 0.0022049446545224782 1106 72-73 0.05515211223700334 1106 74-75 0.15097532691522275 1106 76-77 0.04035160404542637 1106 78-79 0.057722468983037345 1106 80-81 0.11225411090575221 1106 82-83 0.08548220053293676 1106 84-85 0.05276671057796989 1106 86-87 0.11992016930298632 1106 88-89 0.14422884708080375 1106 90-91 0.1782871994841635 1106 92-93 0.24889628741573944 1106 94-95 0.17437395792877197 1106 96-97 0.058374246192993695 1106 98-99 0.1619910032120231 1106 100 0.24982742086275778 1107 1 0.12406587344240805 1107 2 0.05448942075043561 1107 3 0.08778817613962886 1107 4 0.06719651957445194 1107 5 0.06873675727354112 1107 6 0.16765753509826453 1107 7 0.03498826392775811 1107 8 0.05507617384769503 1107 9 0.04543526510516216 1107 10-11 0.048608311881181976 1107 12-13 0.04664968472522446 1107 14-15 -0.04496778402218382 1107 16-17 -0.05136591560263071 1107 18-19 -0.002454619650293921 1107 20-21 0.03255599382930541 1107 22-23 -0.01065459453263884 1107 24-25 -0.0018050807423080073 1107 26-27 0.057700698403579054 1107 28-29 0.038507074848780576 1107 30-31 0.035701709298649575 1107 32-33 0.04240196222348658 1107 34-35 0.0425888504698122 1107 36-37 0.07197605478793179 1107 38-39 0.10934556861383982 1107 40-41 0.09468092120646077 1107 42-43 0.04093485967328547 1107 44-45 0.08340494807367094 1107 46-47 0.09447936929499434 1107 48-49 0.04498833134731228 1107 50-51 -0.036238329181564666 1107 52-53 -0.017385963544420235 1107 54-55 0.010533923721759209 1107 56-57 0.02005922329601617 1107 58-59 0.011603478468437345 1107 60-61 0.032682855341640504 1107 62-63 0.04236332643389318 1107 64-65 -0.021669020395492566 1107 66-67 -0.04370362000939565 1107 68-69 -0.05613833529783108 1107 70-71 -0.015669434667024973 1107 72-73 0.026154536943494122 1107 74-75 0.055461323945245056 1107 76-77 -0.038679325464066494 1107 78-79 -0.004655425582832606 1107 80-81 -0.003846404199357778 1107 82-83 -0.018393559363104828 1107 84-85 -0.09035797038170301 1107 86-87 -0.0876555882727672 1107 88-89 -0.05817932731243758 1107 90-91 -0.07625622480466632 1107 92-93 3.403799490371284E-4 1107 94-95 -0.09315991835319082 1107 96-97 -0.049432670601287754 1107 98-99 -0.038473055444992355 1107 100 0.005311434042020835 1108 1 -0.1711357720930664 1108 2 -0.15647407174124695 1108 3 -0.0481213643693188 1108 4 -0.15309279793296326 1108 5 -0.1842429657729454 1108 6 -0.2246127193752514 1108 7 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-0.05126013060632317 2214 22-23 4.2798629501561436E-4 2214 24-25 -0.07219805148530867 2214 26-27 -0.014548186180192602 2214 28-29 0.038750905369553834 2214 30-31 0.05084922176035178 2214 32-33 -0.003320363179177832 2214 34-35 -0.03555866466035695 2214 36-37 -0.05719081201323206 2214 38-39 -0.003246626674730635 2214 40-41 0.03770863583068973 2214 42-43 0.046071578214814224 2214 44-45 0.09315276527349425 2214 46-47 0.08999809120944491 2214 48-49 0.07812146347877302 2214 50-51 0.056900193238831775 2214 52-53 0.14023775959753237 2214 54-55 0.03480883646312094 2214 56-57 0.10556695115580794 2214 58-59 0.11211081940155054 2214 60-61 0.10571166403441623 2214 62-63 0.040197137421543516 2214 64-65 -0.03128114641084068 2214 66-67 0.03702643058460353 2214 68-69 -0.028311368909747614 2214 70-71 0.09777106401475777 2214 72-73 0.09488986041419167 2214 74-75 0.08432545397396041 2214 76-77 0.16407279062461555 2214 78-79 0.15688559665077406 2214 80-81 0.10021872756919237 2214 82-83 0.13173005704044982 2214 84-85 0.10586942776318153 2214 86-87 0.10622696747135763 2214 88-89 0.14838435014548423 2214 90-91 0.03410560501163573 2214 92-93 0.1206747779978592 2214 94-95 0.09561507177350848 2214 96-97 0.15038313895606947 2214 98-99 0.13805650575346462 2214 100 0.265998348982194 2215 1 -0.00793267737256187 2215 2 0.04141429167427546 2215 3 0.06243704743745582 2215 4 -0.04704328159112947 2215 5 0.0897757813850717 2215 6 0.0379236605280866 2215 7 0.06622014911490481 2215 8 0.06404839520421035 2215 9 0.0042141319359672025 2215 10-11 -0.014213664308556417 2215 12-13 -0.03641510786990665 2215 14-15 -0.09444680460165245 2215 16-17 0.0218743504448895 2215 18-19 -0.06855881813150688 2215 20-21 -0.05557880375226887 2215 22-23 -0.04010700776333209 2215 24-25 0.04209931719663018 2215 26-27 -0.08668637164555548 2215 28-29 0.010417103313059783 2215 30-31 4.940447200496578E-4 2215 32-33 -0.018482118556889304 2215 34-35 -0.03030379296833985 2215 36-37 -0.18345728529787664 2215 38-39 -0.07258454846368778 2215 40-41 -0.0477774731588525 2215 42-43 -0.03734989479633555 2215 44-45 0.015809881016402016 2215 46-47 -0.004771755251816501 2215 48-49 0.025587236926938317 2215 50-51 0.05967389123999567 2215 52-53 -0.14372081099042333 2215 54-55 -0.027869674308597325 2215 56-57 -0.10516919215780263 2215 58-59 -0.08726113180986061 2215 60-61 -0.0935515490381178 2215 62-63 0.04081720443225123 2215 64-65 -0.10546820957906533 2215 66-67 -0.04252381977877917 2215 68-69 0.003943889071337026 2215 70-71 0.045951574879936175 2215 72-73 0.18119467521340482 2215 74-75 0.18225287244359833 2215 76-77 0.07379833942618319 2215 78-79 0.12169253724739093 2215 80-81 0.025544003130718806 2215 82-83 0.0799265676873091 2215 84-85 0.057602976938603945 2215 86-87 -0.10021181561302228 2215 88-89 -0.14034143086423967 2215 90-91 -0.09958959709458881 2215 92-93 -0.09959306921521716 2215 94-95 -0.12384433364079328 2215 96-97 -0.1381661010663784 2215 98-99 -0.15934157956171902 2215 100 -0.224231942818804 2216 1 0.3239513081711678 2216 2 0.26308078397395107 2216 3 0.24605751806019782 2216 4 0.1612360291112367 2216 5 0.23732213420360182 2216 6 0.12981487140410763 2216 7 0.1278201935784935 2216 8 0.16246202142427535 2216 9 0.09530831439919751 2216 10-11 0.12460379119750797 2216 12-13 0.14154069289961058 2216 14-15 0.13604328029950352 2216 16-17 0.153148059296214 2216 18-19 0.003438266981689253 2216 20-21 0.04257735870420731 2216 22-23 0.1061302498237282 2216 24-25 0.08392018967301595 2216 26-27 0.05381218795853471 2216 28-29 0.0759394710175485 2216 30-31 0.11220757173775553 2216 32-33 0.20831414949126525 2216 34-35 0.20801955396250094 2216 36-37 0.14973439431398106 2216 38-39 0.12280750404443808 2216 40-41 0.18551260314850282 2216 42-43 0.16576817236423125 2216 44-45 0.21961737892166155 2216 46-47 0.21531889534568904 2216 48-49 0.24986870869022226 2216 50-51 0.2424736888186061 2216 52-53 0.20752888659181679 2216 54-55 0.21716787219834544 2216 56-57 0.27220706410189166 2216 58-59 0.2411473848345551 2216 60-61 0.29321460333932947 2216 62-63 0.2966399138337792 2216 64-65 0.3756428242085903 2216 66-67 0.33937323161309507 2216 68-69 0.26951706062323666 2216 70-71 0.23124555996595575 2216 72-73 0.4001384152359506 2216 74-75 0.37167334194541013 2216 76-77 0.280572800702231 2216 78-79 0.23179852124236078 2216 80-81 0.2518787630040151 2216 82-83 0.21460381905320958 2216 84-85 0.30535615314236964 2216 86-87 0.26757003306320826 2216 88-89 0.3409511119576436 2216 90-91 0.32561758825783826 2216 92-93 0.3607250509093234 2216 94-95 0.41880544371435136 2216 96-97 0.39941496790915565 2216 98-99 0.42234355755145714 2216 100 0.4543185350246013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19307.0 3 3306.0 4 2112.0 5 1845.0 6 1551.0 7 1614.0 8 1928.0 9 2081.0 10 2337.0 11 2622.0 12 3035.0 13 3273.0 14 3619.0 15 3672.0 16 3768.0 17 3765.0 18 3740.0 19 3789.0 20 3710.0 21 3953.0 22 4514.0 23 6082.0 24 9392.0 25 12866.0 26 13535.0 27 11709.0 28 12200.0 29 15155.0 30 18592.0 31 22436.0 32 29497.0 33 40309.0 34 56211.0 35 86206.0 36 160549.0 37 339426.0 38 547509.0 39 237131.0 40 604.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99986771924355 19.980417723731637 13.350140075872464 23.669574481152356 2 15.566039403607068 19.99756040358232 40.78421828613048 23.652181906680134 3 18.390070742367854 25.130768247625966 29.64559316453564 26.83356784547054 4 12.108677142375983 16.85418995005529 39.27189776938758 31.765235138181147 5 12.779830586822047 37.901679116012986 35.68888509405464 13.629605203110327 6 30.77057563447414 38.307150648499 17.946465940925457 12.9758077761014 7 27.249828850481546 32.37207051772214 22.54140699914705 17.836693632649265 8 24.619716518893934 37.03615835240221 20.39817103646565 17.94595409223821 9 24.38070534655657 17.395294785314718 21.399060181582055 36.82493968654666 10-11 23.553715845675526 27.200209351710285 28.977491158775848 20.26858364383834 12-13 24.322669596044868 24.413270742599728 29.02424507875059 22.239814582604815 14-15 21.579896750933557 26.69472292361229 25.980786654507714 25.744593670946436 16-17 21.261101776637496 29.99763923508862 26.532377802451663 22.208881185822225 18-19 21.10399845992312 28.95606512711963 28.478853231438645 21.461083181518607 20-21 22.45571108505597 28.29109446996808 27.8754714726136 21.37772297236235 22-23 21.871906595407182 28.198633699393262 28.00919187283968 21.920267832359876 24-25 21.82013325141881 28.166870128291215 27.969315612399864 22.04368100789011 26-27 21.581288914216817 28.047583668509745 28.113873242089994 22.257254175183444 28-29 21.63101006116766 28.116548236499256 28.110072353277094 22.142369349055993 30-31 21.93681020277323 28.697242488948234 27.662182377573963 21.703764930704576 32-33 21.447072846130318 28.239345052762154 28.322211385981372 21.991370715126152 34-35 21.556696576270667 27.937909216247498 28.38393358584402 22.121460621637816 36-37 22.054607681313325 28.388388789078228 27.76022915376519 21.796774375843256 38-39 21.7943400919231 28.517353686122206 27.97900162035436 21.709304601600337 40-41 21.47979529961434 28.23483738728278 28.299648776427492 21.98571853667539 42-43 21.419502001412763 28.149016953143395 28.287320461502237 22.144160583941606 44-45 21.677027463346736 28.35506888279109 28.06795301247975 21.899950641382418 46-47 21.71091997613609 28.607731518640072 27.95585941719432 21.72548908802952 48-49 21.63343233048075 28.309632782486954 28.080215649162195 21.9767192378701 50-51 21.634770610636583 28.14682850004312 28.115247858005425 22.10315303131487 52-53 21.63400070166681 28.141165708742342 28.139723524439432 22.08511006515141 54-55 21.823575722347712 28.61343084812856 27.92568666259537 21.637306766928358 56-57 21.583350378701482 28.337966239870273 27.917781973309157 22.160901408119088 58-59 21.432450003487563 27.918259737882334 28.377823565750294 22.27146669287981 60-61 21.81367831375866 28.4308947252865 27.89424673564156 21.86118022531328 62-63 21.587767342890526 28.431745340353583 27.92164907189673 22.058838244859157 64-65 21.56111859957267 28.075148183246906 28.205731910460237 22.15800130672019 66-67 21.445161897922784 28.159672966784587 27.972288114143772 22.422877021148853 68-69 21.95674893290902 28.348729129442706 27.844165720708382 21.850356216939897 70-71 21.897720423423934 28.04436015520521 28.020021048765326 22.03789837260553 72-73 21.628587918101776 28.195579115711304 28.151109517936025 22.024723448250896 74-75 21.662514994873195 28.221087118005972 28.056275950401254 22.060121936719575 76-77 21.712264885334648 28.242749555074127 27.8872208930774 22.15776466651382 78-79 21.872312572437302 28.38735804643404 27.91016214771706 21.8301672334116 80-81 21.73484887057237 28.085254414422582 28.07012690333668 22.10976981166837 82-83 21.556579937554098 28.207312863155533 28.190537406178844 22.04556979311153 84-85 21.5901887045669 28.127540248125406 28.049872299648182 22.232398747659513 86-87 21.78148237312019 28.25663027668901 27.980659375329253 21.981227974861543 88-89 21.564071121648002 28.125516244187722 28.02524274948916 22.285169884675117 90-91 21.53230252494956 27.983895370836976 28.132440974070693 22.351361130142767 92-93 21.712304898741422 28.26890924492108 27.901659817008884 22.117126039328614 94-95 21.491537867193212 27.980515040009003 28.140166193505607 22.387780899292185 96-97 21.489949801553763 28.029039728619924 28.17566525448691 22.305345215339404 98-99 21.473854595530987 28.111001779694433 28.065354926485476 22.349788698289103 100 21.715522937344144 27.95156527155135 27.902661636578497 22.430250154526014 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 274.0 2 248.0 3 225.0 4 180.0 5 154.0 6 124.0 7 106.5 8 126.0 9 133.5 10 126.5 11 132.0 12 146.5 13 168.5 14 182.0 15 216.0 16 305.5 17 403.5 18 539.0 19 821.5 20 1197.5 21 1841.5 22 2811.0 23 4229.0 24 6141.0 25 8338.5 26 11139.5 27 14744.5 28 19453.0 29 24432.0 30 29158.0 31 34158.5 32 39298.5 33 44305.0 34 49004.0 35 53074.0 36 55970.5 37 58384.0 38 60164.0 39 61024.5 40 61888.5 41 61908.0 42 60913.0 43 59744.0 44 58260.5 45 56823.5 46 56140.5 47 54901.0 48 53147.5 49 51992.5 50 50776.5 51 49172.0 52 46281.5 53 43380.5 54 41456.0 55 39546.5 56 36972.5 57 33871.5 58 30988.5 59 28280.5 60 25435.5 61 22085.5 62 18761.0 63 15718.5 64 12967.0 65 10483.0 66 8138.0 67 6165.0 68 4638.0 69 3573.5 70 2745.0 71 2051.0 72 1560.5 73 1217.5 74 970.0 75 711.5 76 461.0 77 334.0 78 248.0 79 180.5 80 143.5 81 82.0 82 50.0 83 38.0 84 24.5 85 18.0 86 18.5 87 15.0 88 9.5 89 6.5 90 5.5 91 3.5 92 2.0 93 2.5 94 1.5 95 0.5 96 2.0 97 1.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3285558727449307 2 0.11465905412166338 3 0.06468701256658524 4 0.039553842078931103 5 0.0432620147738309 6 0.010653639012331146 7 0.008416963418582064 8 0.016657347185026046 9 0.01907060243091321 10-11 0.021954736749168605 12-13 0.017422525677624413 14-15 0.019953500691603638 16-17 0.020483239648017893 18-19 0.01962977132935048 20-21 0.024456281821124814 22-23 0.016215898054680832 24-25 0.02539803996586127 26-27 0.02013008034374172 28-29 0.020012360575649667 30-31 0.020777539068248037 32-33 0.02539803996586127 34-35 0.01624532799670385 36-37 0.01047705936019306 38-39 0.014567821301392036 40-41 0.010271049766031961 42-43 0.008828982606904264 44-45 0.009329291621295506 46-47 0.008858412548927278 48-49 0.00467936078165926 50-51 0.007328055563730539 52-53 0.008122663998351924 54-55 0.003678742752876777 56-57 0.0030901439124164927 58-59 0.0033255834486006068 60-61 0.004179051767268019 62-63 0.0032372936225315637 64-65 0.002354395361841137 66-67 0.002354395361841137 68-69 0.0032667235645545776 70-71 0.0022366755937490803 72-73 0.0021483857676800376 74-75 0.0021189558256570233 76-77 0.004208481709291032 78-79 0.003943612231083905 80-81 0.0034433032166926634 82-83 0.002324965419818123 84-85 0.00300185408634745 86-87 0.0022955354777951086 88-89 0.5926601724594602 90-91 0.6029017922834692 92-93 0.5991641896465464 94-95 0.585950145678213 96-97 0.5858618558521439 98-99 0.5824774125194974 100 0.5835368904323258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1698950.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.44831528765198 #Duplication Level Percentage of deduplicated Percentage of total 1 84.12119838081881 64.30923896191956 2 11.017086715953681 16.844754376252585 3 2.5632789380249523 5.878750692729879 4 0.9088725698709529 2.779271071111724 5 0.4349939918854233 1.6627278919945583 6 0.24300617682359035 1.1146447693594048 7 0.15876198142566755 0.8495960208205247 8 0.11191831217780832 0.6844773132664761 9 0.07463424976310334 0.513509639143238 >10 0.35167078174180866 4.477735809424032 >50 0.012189996290367738 0.6053728414555161 >100 0.002310908147979912 0.24860551391552932 >500 7.699707584775853E-5 0.03131509860698723 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.885988404602843E-5 0.0 4 0.0 0.0 0.0 5.885988404602843E-5 0.0 5 0.0 0.0 0.0 5.885988404602843E-5 0.0 6 0.0 0.0 0.0 5.885988404602843E-5 0.0 7 0.0 0.0 0.0 5.885988404602843E-5 0.0 8 0.0 0.0 0.0 5.885988404602843E-5 0.0 9 0.0 0.0 0.0 1.765796521380853E-4 0.0 10-11 0.0 0.0 0.0 1.765796521380853E-4 0.0 12-13 0.0 0.0 0.0 1.765796521380853E-4 0.0 14-15 0.0 0.0 0.0 2.060095941610995E-4 0.0 16-17 0.0 0.0 0.0 2.3543953618411373E-4 0.0 18-19 0.0 0.0 0.0 2.3543953618411373E-4 0.0 20-21 0.0 0.0 0.0 2.3543953618411373E-4 0.0 22-23 0.0 0.0 0.0 2.6486947820712796E-4 0.0 24-25 0.0 0.0 0.0 3.825892462991848E-4 0.0 26-27 0.0 0.0 0.0 6.180287824832985E-4 0.0 28-29 0.0 0.0 0.0 0.0012654875069896111 0.0 30-31 0.0 0.0 0.0 0.004031902057152948 0.0 32-33 0.0 0.0 0.0 0.009976750345801817 0.0 34-35 0.0 0.0 0.0 0.018599723358544984 0.0 36-37 0.0 0.0 0.0 0.03399158303658142 0.0 38-39 0.0 0.0 0.0 0.06268577650902027 0.0 40-41 0.0 0.0 0.0 0.09944377409576503 0.0 42-43 0.0 0.0 0.0 0.13679037052297005 0.0 44-45 0.0 0.0 0.0 0.17702110126843051 0.0 46-47 0.0 0.0 0.0 0.22781718120015304 0.0 48-49 0.0 0.0 0.0 0.28544100768121483 0.0 50-51 0.0 0.0 0.0 0.3550722505076665 0.0 52-53 0.0 0.0 0.0 0.4201124223785279 0.0 54-55 0.0 0.0 0.0 0.49142117190029133 0.0 56-57 0.0 0.0 0.0 0.573295270608317 0.0 58-59 0.0 0.0 0.0 0.6719444362694605 0.0 60-61 0.0 0.0 0.0 0.7772741987698284 0.0 62-63 0.0 0.0 0.0 0.882015362429736 0.0 64-65 0.0 0.0 0.0 0.9975278848700668 0.0 66-67 0.0 0.0 0.0 1.1271079195973983 0.0 68-69 0.0 0.0 0.0 1.2684599311339357 0.0 70-71 0.0 0.0 0.0 1.4257041113629008 0.0 72-73 0.0 0.0 0.0 1.5967803643426821 0.0 74-75 0.0 0.0 0.0 1.7667088495835663 0.0 76-77 0.0 0.0 0.0 1.9473498337208275 0.0 78-79 0.0 0.0 0.0 2.15739132993908 0.0 80-81 0.0 0.0 0.0 2.3920068277465494 0.0 82-83 0.0 0.0 0.0 2.6387768916095236 0.0 84-85 0.0 0.0 0.0 2.881338473763207 0.0 86-87 0.0 0.0 0.0 3.13493628417552 0.0 88 0.0 0.0 0.0 3.345478089408164 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1550 0.0 33.733494 1 GTACGAC 135 1.333683E-8 31.319407 3 TCAACGC 2220 0.0 30.47204 4 GTATCAA 2350 0.0 29.866781 1 ATCAACG 2310 0.0 28.878931 3 CAACGCA 2415 0.0 28.01156 5 AACGCAG 2510 0.0 26.757898 6 GTACATG 4475 0.0 26.105299 1 TACATGG 4540 0.0 25.264307 2 TATCAAC 2730 0.0 24.967712 2 ACATGGG 4675 0.0 23.715656 3 ATAACGG 100 0.001766301 23.483332 8 CGCAGAG 2840 0.0 22.491077 8 ACGCAGA 2920 0.0 22.357418 7 TGTACGA 190 3.635796E-7 22.26703 2 CATGGGG 3100 0.0 19.548796 4 GTGTACG 220 1.4574689E-6 19.27036 1 GCGTAGT 150 8.1687793E-4 18.84213 1 TAAGCCG 155 0.0010368953 18.184927 5 CTGTCGC 440 0.0 18.14621 9 >>END_MODULE