FastQCFastQC Report
Wed 25 May 2016
SRR1033106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698950
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.19200094175814475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.16539627416933989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.042.5577471
GTATCAA51650.038.0808261
ATCAACG58300.032.9776883
TCAACGC58300.032.9776884
CAACGCA59300.032.421575
AACGCAG61250.031.3792136
TATCAAC62900.030.7901672
ACGCAGA71500.026.8150737
CGCAGAG73000.026.1997038
GTACATG45800.024.143641
GCAGAGT79050.023.8973089
TACATGG46250.022.9700622
GAGTACT51950.021.07634212-13
ACATGGG49200.020.637363
GGACCGT1605.4218304E-520.5590426
AGAGTAC70200.020.55067610-11
TATACCG1200.005060552619.586385
CAGAGTA77900.019.39404310-11
AGTACTT55900.019.12469112-13
CATGGGG28350.018.73664
Produced by FastQC (version 0.11.5)