##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033106_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1698950
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72993908002001	34.0	31.0	34.0	31.0	34.0
2	32.83794520144795	34.0	31.0	34.0	31.0	34.0
3	32.926948409311635	34.0	31.0	34.0	31.0	34.0
4	36.35547014332382	37.0	37.0	37.0	35.0	37.0
5	36.29934076929869	37.0	37.0	37.0	35.0	37.0
6	36.387255069307514	37.0	37.0	37.0	35.0	37.0
7	36.35913829129757	37.0	37.0	37.0	35.0	37.0
8	36.35562612201654	37.0	37.0	37.0	35.0	37.0
9	38.1679037052297	39.0	39.0	39.0	37.0	39.0
10-11	38.15276288295712	39.0	39.0	39.0	37.0	39.0
12-13	38.11904764707614	39.0	39.0	39.0	37.0	39.0
14-15	39.644478354277645	41.0	40.0	41.0	37.0	41.0
16-17	39.65367285676447	41.0	40.0	41.0	37.0	41.0
18-19	39.671971511816125	41.0	40.0	41.0	37.0	41.0
20-21	39.66904382118367	41.0	40.0	41.0	37.0	41.0
22-23	39.59750728391065	41.0	40.0	41.0	37.0	41.0
24-25	39.54933547190912	41.0	40.0	41.0	37.0	41.0
26-27	39.44926219135348	41.0	39.0	41.0	36.5	41.0
28-29	39.35806409841372	41.0	39.0	41.0	36.0	41.0
30-31	39.26101209570617	41.0	39.0	41.0	36.0	41.0
32-33	39.29081726948998	41.0	39.0	41.0	35.5	41.0
34-35	39.23347479325466	41.0	39.0	41.0	35.0	41.0
36-37	39.142513905647604	41.0	39.0	41.0	35.0	41.0
38-39	39.04717089967333	41.0	39.0	41.0	35.0	41.0
40-41	38.92731128049678	41.0	39.0	41.0	35.0	41.0
42-43	38.80916566114365	41.0	39.0	41.0	35.0	41.0
44-45	38.65613967450484	41.0	39.0	41.0	35.0	41.0
46-47	38.44494805615233	41.0	38.5	41.0	35.0	41.0
48-49	38.257281850554754	41.0	38.0	41.0	34.5	41.0
50-51	38.12443832955649	40.5	38.0	41.0	34.0	41.0
52-53	37.98920804026016	40.0	38.0	41.0	34.0	41.0
54-55	37.855613172842055	40.0	38.0	41.0	34.0	41.0
56-57	37.702505076665	40.0	37.0	41.0	33.5	41.0
58-59	37.51763471555961	40.0	37.0	41.0	33.0	41.0
60-61	37.32844904205538	40.0	36.5	41.0	33.0	41.0
62-63	37.095171135112864	39.5	36.0	41.0	33.0	41.0
64-65	36.841481797580855	39.0	35.0	41.0	32.5	41.0
66-67	36.570423791165126	39.0	35.0	41.0	32.0	41.0
68-69	36.25478059978222	38.0	35.0	40.5	32.0	41.0
70-71	35.920907325112566	37.0	35.0	40.0	31.5	41.0
72-73	35.56879602107184	37.0	35.0	39.5	31.0	41.0
74-75	35.234193178139435	36.0	35.0	39.0	31.0	41.0
76-77	34.89638247152653	36.0	35.0	39.0	31.0	40.5
78-79	34.55578327790694	35.5	35.0	37.5	31.0	39.5
80-81	34.21397598516731	35.0	35.0	37.0	30.5	39.0
82-83	33.892349980870534	35.0	34.0	37.0	30.0	39.0
84-85	33.614358868713026	35.0	34.0	36.0	30.0	38.0
86-87	33.37880808734807	35.0	34.0	36.0	30.0	37.0
88-89	33.157321580976486	35.0	34.0	36.0	30.0	37.0
90-91	32.95819594455399	35.0	34.0	35.0	29.5	36.5
92-93	32.790289884928924	35.0	34.0	35.0	29.0	36.0
94-95	32.63768003767032	35.0	34.0	35.0	29.0	36.0
96-97	32.509876688542924	35.0	34.0	35.0	29.0	36.0
98-99	32.403393566614675	35.0	34.0	35.0	29.0	36.0
100	32.30490597133524	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1787118716911067
1101	2	0.2002711817109315
1101	3	0.1745728704344529
1101	4	0.12664694747287797
1101	5	0.09047066966525819
1101	6	0.1260461970029496
1101	7	0.1728690319822377
1101	8	0.1359802016366629
1101	9	0.18429534703453498
1101	10-11	0.15331354280183973
1101	12-13	0.18214574639005576
1101	14-15	0.2425419866328724
1101	16-17	0.20839698232256154
1101	18-19	0.24880957958032468
1101	20-21	0.24381761553143377
1101	22-23	0.2727043908536686
1101	24-25	0.3244840902502304
1101	26-27	0.23389083391346333
1101	28-29	0.24958668035490206
1101	30-31	0.2811553129438451
1101	32-33	0.18155630777323495
1101	34-35	0.22742413423220142
1101	36-37	0.2429173242267879
1101	38-39	0.19523602890890857
1101	40-41	0.2098317617393164
1101	42-43	0.09729985745455849
1101	44-45	0.0992936472427175
1101	46-47	0.20118190026482807
1101	48-49	0.1658944947016039
1101	50-51	0.2359255847499
1101	52-53	0.19456987663190262
1101	54-55	0.21360988074911091
1101	56-57	0.1479412409620835
1101	58-59	0.15489225664682493
1101	60-61	0.17408879481025252
1101	62-63	0.21193167693780168
1101	64-65	0.18692824597672342
1101	66-67	0.28239218302663005
1101	68-69	0.20923153029094266
1101	70-71	0.19710769166580633
1101	72-73	0.2862868900482809
1101	74-75	0.2992186631757221
1101	76-77	0.2894201571964956
1101	78-79	0.2767228652981686
1101	80-81	0.18002822571772725
1101	82-83	0.2333889552075803
1101	84-85	0.22894855558964622
1101	86-87	0.22917741919698642
1101	88-89	0.24740912548493554
1101	90-91	0.2585852348091322
1101	92-93	0.19611976136863518
1101	94-95	0.19842108180334606
1101	96-97	0.27582257180604586
1101	98-99	0.2916699020345419
1101	100	0.281428869749341
1102	1	0.08446450325018873
1102	2	0.13022019230510296
1102	3	0.0793083558151011
1102	4	0.06673533917417274
1102	5	0.053938326681304716
1102	6	0.02741887256917863
1102	7	0.08852138311846147
1102	8	0.14961826366182152
1102	9	0.17129889855421254
1102	10-11	0.18945525636290483
1102	12-13	0.20787161889012395
1102	14-15	0.20064367860553034
1102	16-17	0.17618892592096103
1102	18-19	0.19846744859302845
1102	20-21	0.2531946087794026
1102	22-23	0.2852941695724809
1102	24-25	0.33571327083425473
1102	26-27	0.31494926981060445
1102	28-29	0.27739779687569666
1102	30-31	0.24496736546557685
1102	32-33	0.24385226523048686
1102	34-35	0.31307105052427886
1102	36-37	0.21994001092689075
1102	38-39	0.26107123990540515
1102	40-41	0.24514126479075316
1102	42-43	0.24971987277779562
1102	44-45	0.23990231204492574
1102	46-47	0.21707976293689057
1102	48-49	0.26883950087241004
1102	50-51	0.31662641545072745
1102	52-53	0.36733281552386643
1102	54-55	0.3020902756811239
1102	56-57	0.24190931880112743
1102	58-59	0.2604905235354167
1102	60-61	0.3242254207533293
1102	62-63	0.3325144507463804
1102	64-65	0.24376105280953198
1102	66-67	0.2638957854979722
1102	68-69	0.2328938604048858
1102	70-71	0.25922909249688075
1102	72-73	0.2962413354865916
1102	74-75	0.3105969245539839
1102	76-77	0.22739877259446217
1102	78-79	0.23806847986958957
1102	80-81	0.1663854459459202
1102	82-83	0.12529971808607598
1102	84-85	0.2213614754219151
1102	86-87	0.24493469301877724
1102	88-89	0.2287126954981744
1102	90-91	0.20326210690535618
1102	92-93	0.2719707485099434
1102	94-95	0.20219810977391717
1102	96-97	0.20604992945276024
1102	98-99	0.28862695544321326
1102	100	0.34382012568898546
1103	1	0.14702027476118218
1103	2	0.1542340297517768
1103	3	0.1273360307084488
1103	4	0.06828777356243165
1103	5	0.020622106797915762
1103	6	0.07309956004090878
1103	7	0.09818437039338335
1103	8	0.09591259055316215
1103	9	0.15295694737643828
1103	10-11	0.11814717759213522
1103	12-13	0.0818096682013092
1103	14-15	0.11292196774949304
1103	16-17	0.1763753402864765
1103	18-19	0.1461980828908267
1103	20-21	0.105933371965925
1103	22-23	0.12363013104277343
1103	24-25	0.17196505857857147
1103	26-27	0.17869564848236053
1103	28-29	0.20850088096385377
1103	30-31	0.2523230929705562
1103	32-33	0.24537719585988782
1103	34-35	0.2670572619731786
1103	36-37	0.23276898644243005
1103	38-39	0.2575690946122222
1103	40-41	0.29998375993175586
1103	42-43	0.26014990932723947
1103	44-45	0.29023724670822304
1103	46-47	0.39937162060188314
1103	48-49	0.31951976050789455
1103	50-51	0.2680578333000625
1103	52-53	0.3107759194147377
1103	54-55	0.28571637124751703
1103	56-57	0.32025224789032336
1103	58-59	0.3091416169626271
1103	60-61	0.32591537393582115
1103	62-63	0.28453872739752484
1103	64-65	0.17628822041132963
1103	66-67	0.25614278147631353
1103	68-69	0.2456617164563255
1103	70-71	0.2567904258791103
1103	72-73	0.23259152556813234
1103	74-75	0.2720397106236305
1103	76-77	0.20437507046629122
1103	78-79	0.26115024548867183
1103	80-81	0.2067212362817088
1103	82-83	0.22876274692224996
1103	84-85	0.29241590841665044
1103	86-87	0.21549955910602847
1103	88-89	0.1886213830188055
1103	90-91	0.18298572468867746
1103	92-93	0.17724474706751892
1103	94-95	0.2196324369097411
1103	96-97	0.26792421893898677
1103	98-99	0.2742058178877471
1103	100	0.2175839845274652
1104	1	0.0446647334975907
1104	2	0.13826339846151825
1104	3	0.17027153168393738
1104	4	0.08421733660900088
1104	5	0.019163629170755314
1104	6	0.022529012726486997
1104	7	0.08538059224181893
1104	8	0.1041173596703473
1104	9	0.13429705966558458
1104	10-11	0.13772975298645918
1104	12-13	0.1546167666965914
1104	14-15	0.17445979746882045
1104	16-17	0.16323992417478195
1104	18-19	0.1661632747794286
1104	20-21	0.15171915176149042
1104	22-23	0.1924467123382314
1104	24-25	0.17723709525901654
1104	26-27	0.10417969878160704
1104	28-29	0.11270571414115693
1104	30-31	0.11505867246725643
1104	32-33	0.08500039234559154
1104	34-35	0.1464208025061069
1104	36-37	0.1291318464158948
1104	38-39	0.1978005292290419
1104	40-41	0.24679182186583404
1104	42-43	0.24120294032218226
1104	44-45	0.23800161203102022
1104	46-47	0.22884517328720477
1104	48-49	0.15577440962336198
1104	50-51	0.21070198759234415
1104	52-53	0.20414159042226743
1104	54-55	0.15992457868051702
1104	56-57	0.23496809369952132
1104	58-59	0.23595816527663516
1104	60-61	0.1867351753621378
1104	62-63	0.22827824627432136
1104	64-65	0.19472096426472518
1104	66-67	0.34037240147930703
1104	68-69	0.23762629438260774
1104	70-71	0.24613908353423142
1104	72-73	0.29923339272394145
1104	74-75	0.29286043806749973
1104	76-77	0.20538909138667094
1104	78-79	0.2268275004682323
1104	80-81	0.3055388038492737
1104	82-83	0.25195124096402566
1104	84-85	0.295284025759571
1104	86-87	0.2629377410925784
1104	88-89	0.2609304299564741
1104	90-91	0.25784155771122386
1104	92-93	0.3249706287503855
1104	94-95	0.26450591500625364
1104	96-97	0.30073386081122067
1104	98-99	0.2676980076274731
1104	100	0.2822038352991072
1105	1	0.039480556240533815
1105	2	0.028463887003802313
1105	3	0.07612423887952247
1105	4	0.013145183519348791
1105	5	0.08825261050863276
1105	6	0.04914265393455963
1105	7	-0.04688414671636565
1105	8	0.006007092197123143
1105	9	0.04059935316826113
1105	10-11	-0.01422230298274485
1105	12-13	-0.0022005756970671086
1105	14-15	-0.0019196791000126723
1105	16-17	0.02930661668960255
1105	18-19	0.027745818580399373
1105	20-21	0.03603528137464451
1105	22-23	0.0714717041917794
1105	24-25	0.042006480146547176
1105	26-27	0.07034687444857468
1105	28-29	0.007207209291266281
1105	30-31	0.013851603671980683
1105	32-33	-0.01656806053850346
1105	34-35	0.0472488927075716
1105	36-37	-0.017945429140084457
1105	38-39	-0.05129493864697565
1105	40-41	-0.013133530881781041
1105	42-43	-0.015812428301842374
1105	44-45	0.0974764903242118
1105	46-47	0.06243178174040054
1105	48-49	0.018431994524121365
1105	50-51	0.011293769350650962
1105	52-53	0.01692832419611534
1105	54-55	0.00923223720562305
1105	56-57	0.015222526473507969
1105	58-59	0.040586729810037525
1105	60-61	-0.013331573504096639
1105	62-63	0.040071097239525955
1105	64-65	-0.004742763185518584
1105	66-67	-0.009053625647773345
1105	68-69	-0.08252386989403249
1105	70-71	-0.10344772888660714
1105	72-73	-0.0793994361417063
1105	74-75	-0.1306681621142971
1105	76-77	-0.1524796183863728
1105	78-79	-0.17179094041055265
1105	80-81	-0.08352187008917156
1105	82-83	-0.05192255146185687
1105	84-85	-0.08305326442328465
1105	86-87	-0.14649090760043748
1105	88-89	-0.11908308519592481
1105	90-91	-0.12352217108655594
1105	92-93	0.04457080013219894
1105	94-95	0.07420626021993826
1105	96-97	-0.010463242358426328
1105	98-99	-0.017517034280828625
1105	100	-0.09920858847335268
1106	1	0.05189009395296296
1106	2	0.06798302823026603
1106	3	0.09296986641382432
1106	4	0.04291846926236076
1106	5	-0.01820743038022954
1106	6	0.07780184638247079
1106	7	0.03058993085316075
1106	8	0.059678051975822655
1106	9	0.07754399928629852
1106	10-11	0.05029645047362408
1106	12-13	0.11389488077096388
1106	14-15	0.11532476997115992
1106	16-17	0.19096543150909895
1106	18-19	0.11406097961535266
1106	20-21	0.08164435185395291
1106	22-23	0.14982713843163253
1106	24-25	0.14558859583208772
1106	26-27	0.135777633326569
1106	28-29	0.08917889789876199
1106	30-31	0.08443411190016548
1106	32-33	0.0992509605985532
1106	34-35	0.0655224090246378
1106	36-37	0.0017062369886744477
1106	38-39	0.07892429761298558
1106	40-41	-0.023874165786700985
1106	42-43	-0.037203988992928316
1106	44-45	-0.020138130039917712
1106	46-47	-0.01075892776550802
1106	48-49	0.07930555999850952
1106	50-51	0.03965363311807124
1106	52-53	0.04851040826348196
1106	54-55	0.04930216110127361
1106	56-57	0.08841360883528182
1106	58-59	0.036581437643171455
1106	60-61	0.1039758136354223
1106	62-63	0.04277676303778577
1106	64-65	0.011143655375896344
1106	66-67	0.0796541514094784
1106	68-69	0.08335283950092531
1106	70-71	0.08286297279232713
1106	72-73	0.15070266372281083
1106	74-75	0.11730849830230738
1106	76-77	0.10189301136895068
1106	78-79	0.12940522591537018
1106	80-81	0.09913566449245792
1106	82-83	0.2050607404198459
1106	84-85	0.19932151119206765
1106	86-87	0.2264301972937588
1106	88-89	0.2203295385698425
1106	90-91	0.16800822919359604
1106	92-93	0.08197055410698795
1106	94-95	0.12014799269317677
1106	96-97	0.14752256943229014
1106	98-99	0.15504908201081946
1106	100	0.17782226788408195
1107	1	-0.05738643860075143
1107	2	-0.03347759639268588
1107	3	-0.02070188169513898
1107	4	-0.01941330947447284
1107	5	-0.10618657094359207
1107	6	-0.13520397755367242
1107	7	-0.07709081999374234
1107	8	-0.03311105656749902
1107	9	-0.08592878367346657
1107	10-11	-0.08707013266248964
1107	12-13	-0.014451272182768093
1107	14-15	-0.053727738265884284
1107	16-17	-0.07239829766626826
1107	18-19	-0.03372952110393612
1107	20-21	-0.06598024789546031
1107	22-23	-0.032135191606883495
1107	24-25	-0.04038726151627969
1107	26-27	-4.4794558660754547E-4
1107	28-29	0.006559344787248733
1107	30-31	-0.006271373273165182
1107	32-33	0.04105742493564435
1107	34-35	0.012722975176203022
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2216	20-21	-0.24224356223778898
2216	22-23	-0.27895471518048964
2216	24-25	-0.3328357587501998
2216	26-27	-0.3600611071752482
2216	28-29	-0.41193365744213395
2216	30-31	-0.41235262172498466
2216	32-33	-0.26130303249974673
2216	34-35	-0.34495496223605926
2216	36-37	-0.39219364241929355
2216	38-39	-0.4340307390862108
2216	40-41	-0.38879675112955425
2216	42-43	-0.3439367482970823
2216	44-45	-0.19840804128585887
2216	46-47	-0.15733248681596024
2216	48-49	-0.15857264622638212
2216	50-51	-0.1826357585389644
2216	52-53	-0.17086537151628534
2216	54-55	-0.21970725258779567
2216	56-57	-0.13013310075491802
2216	58-59	-0.1424810323653034
2216	60-61	-0.1573586372953102
2216	62-63	-0.19764994079402953
2216	64-65	-0.11895881655203056
2216	66-67	-0.0811317414096564
2216	68-69	-0.07778486699727694
2216	70-71	-0.03189557192479242
2216	72-73	0.019585174123356808
2216	74-75	-0.08875286801787752
2216	76-77	8.087784636003903E-4
2216	78-79	-0.014352258927168293
2216	80-81	-0.03582699996543681
2216	82-83	-0.021346618614749957
2216	84-85	-0.03686256481847039
2216	86-87	-0.03413620968973419
2216	88-89	-9.375331302550194E-4
2216	90-91	-0.015513068443389955
2216	92-93	-0.10895682570782128
2216	94-95	-0.19340327560948367
2216	96-97	-0.10412696468189608
2216	98-99	-0.0802928840147743
2216	100	-0.12279248022880296
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	5.0
4	7.0
5	20.0
6	47.0
7	87.0
8	199.0
9	372.0
10	658.0
11	1126.0
12	1783.0
13	2712.0
14	3475.0
15	4419.0
16	5526.0
17	8067.0
18	9126.0
19	4948.0
20	3256.0
21	3164.0
22	3139.0
23	3282.0
24	3783.0
25	4549.0
26	5998.0
27	7511.0
28	9675.0
29	12363.0
30	16069.0
31	20498.0
32	26389.0
33	34574.0
34	47996.0
35	74416.0
36	139254.0
37	323740.0
38	604936.0
39	310396.0
40	1379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.061476752379676	19.887149647791894	13.58346118405083	23.467912415777604
2	15.881711360510062	20.153167083497898	40.285438649878834	23.679682906113204
3	18.431501809941434	25.242061273139292	30.072221077724475	26.254215839194796
4	12.272291974824318	16.9220708664986	38.9518529483955	31.85378421028158
5	12.449147467486469	37.608057031383865	35.81425584395625	14.128539657173409
6	30.627753913308602	38.42093862900881	17.498260686331406	13.453046771351179
7	26.899296718096267	32.67617953348418	22.44439316919498	17.980130579224575
8	25.08003496287402	36.13836822263228	20.2706385433313	18.510958271162398
9	24.786132107789182	16.445666901714358	21.60722000581537	37.16098098468109
10-11	23.827543411537818	26.67028067737391	28.80251756459235	20.699658346495927
12-13	24.86309190970894	23.97580858765708	29.175461314341213	21.985638188292768
14-15	21.87464776007042	26.002701082463652	26.736537334710263	25.38611382275566
16-17	20.8806910150387	29.53983342652815	27.16062862356161	22.41884693487154
18-19	20.552889036954326	27.936956299734806	30.22437113308614	21.285783530224727
20-21	21.895111686629978	27.19977044645222	29.867476971070367	21.037640895847435
22-23	21.360325354565315	27.126957794138896	30.015335974381774	21.497380876914015
24-25	21.305419589063348	27.155392631204723	29.971341088720788	21.567846691011145
26-27	21.036884783941378	27.132826012676837	30.073625947694588	21.756663255687197
28-29	21.069719678487196	27.204087164829616	30.123826990110263	21.602366166572924
30-31	21.379802516081376	27.697827641566008	29.630684051343813	21.291685791008796
32-33	20.9359386940697	27.31171894226546	30.24666499460111	21.505677369063726
34-35	21.06289175609176	27.034452751403933	30.219489390865274	21.68316610163903
36-37	21.60783265498131	27.53781471540125	29.51782153326594	21.3365310963515
38-39	21.42109212471147	27.664201839685866	29.588756879981798	21.32594915562087
40-41	21.13807902392668	27.413290296673864	29.79191555331292	21.65671512608654
42-43	21.228474033428906	27.362896151377956	29.62090749059481	21.78772232459833
44-45	21.39286669930226	27.646595973223114	29.37574272712474	21.584794600349884
46-47	21.500529684246963	27.9321533202622	29.011596538081484	21.555720457409354
48-49	21.494098601913173	27.786548933039	28.83985283296542	21.879499632082414
50-51	21.523730420864943	27.731219070279156	28.754258125241712	21.990792383614185
52-53	21.595573006571207	27.79844371238081	28.692195104866496	21.913788176181484
54-55	21.704793244727924	28.214727286586665	28.520575755622602	21.55990371306281
56-57	21.368238520413417	27.892698069994417	28.629789623563855	22.10927378602831
58-59	21.251987647522956	27.48693957757542	29.082090173788743	22.178982601112875
60-61	21.61882893901955	27.90787539039094	28.593009279914867	21.880286390674645
62-63	21.44300072104419	27.88969495416219	28.640905278337776	22.026399046455847
64-65	21.40353004469946	27.594558132252477	28.890100782740852	22.11181104030721
66-67	21.3017321347849	27.699941846229187	28.604533168838998	22.393792850146916
68-69	21.877136450602688	27.830135788128256	28.527691048531327	21.765036712737725
70-71	21.832244568462695	27.62148947272231	28.528818056633433	22.017447902181562
72-73	21.585770194491747	27.787721880865785	28.643666848937073	21.982841075705394
74-75	21.633930568472106	27.78938632323684	28.572050808150966	22.004632300140088
76-77	21.619723781360655	27.86321974120395	28.347343997212388	22.16971248022301
78-79	21.823710908161992	27.989879619623437	28.39133393135046	21.79507554086411
80-81	21.68188746312767	27.684260123906927	28.550919943152675	22.082932469812732
82-83	21.462563311721755	27.835496943673537	28.68046746932637	22.021472275278338
84-85	21.447358314152297	27.690561810913668	28.55939588338282	22.30268399155122
86-87	21.590448913092104	27.872660022555134	28.42735622279199	22.109534841560777
88-89	21.323523352658995	27.7580564466288	28.520497954619028	22.397922246093174
90-91	21.248296004350927	27.618385024144352	28.573064037274833	22.56025493422989
92-93	21.458684592263978	27.878361233433502	28.33449630756906	22.32845786673345
94-95	21.239530298125313	27.532829100326673	28.584772947997294	22.64286765355072
96-97	21.127490508843696	27.497101150710733	28.662409135054006	22.712999205391565
98-99	21.147561729303394	27.58494952764943	28.563465669972633	22.70402307307455
100	21.31039759851673	27.52553047470496	28.304541040054147	22.85953088672415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	26.0
2	94.0
3	188.0
4	138.0
5	79.5
6	199.5
7	484.0
8	859.0
9	1041.0
10	833.5
11	602.0
12	625.0
13	736.0
14	844.0
15	971.5
16	1153.0
17	1306.5
18	1515.0
19	1827.5
20	2197.5
21	2776.0
22	3699.0
23	5098.5
24	6832.0
25	8720.5
26	11371.5
27	14913.0
28	19267.5
29	24149.5
30	28867.0
31	33768.5
32	38742.0
33	43994.0
34	48677.0
35	52130.0
36	54986.5
37	57700.5
38	59689.5
39	60578.5
40	61464.0
41	61734.5
42	60559.5
43	59312.5
44	57975.0
45	56541.5
46	55631.5
47	54490.5
48	53512.0
49	52277.0
50	50549.0
51	48511.5
52	45545.5
53	43077.5
54	41281.0
55	39226.5
56	36595.0
57	33613.0
58	30891.0
59	28187.5
60	25289.5
61	22033.0
62	18820.5
63	15676.5
64	12815.0
65	10156.5
66	7910.5
67	6044.5
68	4498.0
69	3486.0
70	2592.0
71	1885.0
72	1411.0
73	1053.5
74	822.5
75	600.0
76	379.5
77	287.5
78	203.5
79	132.0
80	91.5
81	41.0
82	22.5
83	12.0
84	5.0
85	2.5
86	2.0
87	0.5
88	0.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09758968774831513
2	0.001236057564966597
3	0.0
4	5.297389564142558E-4
5	0.038670943818240676
6	2.354395361841137E-4
7	0.0034138732746696486
8	2.942994202301421E-4
9	2.354395361841137E-4
10-11	3.825892462991848E-4
12-13	0.0
14-15	8.828982606904265E-5
16-17	0.0
18-19	8.828982606904265E-5
20-21	0.0
22-23	2.060095941610995E-4
24-25	1.1771976809205685E-4
26-27	0.0014420671591276965
28-29	0.00991789046175579
30-31	0.03734659642720504
32-33	0.026604667588804854
34-35	0.029900821095382443
36-37	0.027723005385679393
38-39	0.026692957414873892
40-41	0.03254951587745372
42-43	0.02472115129933194
44-45	0.023279084140204243
46-47	0.01745195561964743
48-49	0.011771976809205687
50-51	0.008181523882397951
52-53	0.005856558462579828
54-55	0.004090761941198976
56-57	0.0025309750139792223
58-59	0.0011477677388975543
60-61	7.063186085523412E-4
62-63	0.0014714971011507109
64-65	0.0028252744342093647
66-67	3.531593042761706E-4
68-69	9.711880867594691E-4
70-71	3.825892462991848E-4
72-73	5.297389564142558E-4
74-75	5.885988404602842E-4
76-77	3.531593042761706E-4
78-79	1.1771976809205685E-4
80-81	7.946084346213837E-4
82-83	8.828982606904264E-4
84-85	3.825892462991848E-4
86-87	1.1771976809205685E-4
88-89	0.0
90-91	1.1771976809205685E-4
92-93	8.828982606904265E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1698950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.57724067002279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.21946324429517	62.06277938799916
2	11.61355006930189	17.32213037103374
3	2.690013794173387	6.018414184012496
4	0.9868701460446583	2.9439220956653207
5	0.4551179685383301	1.6970721136467453
6	0.26242417920745326	1.174252270223246
7	0.1699065373418392	0.886981250872679
8	0.11308312024637013	0.6746741659464538
9	0.08211885738201079	0.5511778011472946
>10	0.38779732656317234	4.893243377277928
>50	0.014993208774819168	0.7199917717219123
>100	0.004345599156832466	0.5620781233807526
>500	7.898724351661346E-5	0.05195553890587357
>1k	2.3696173054984037E-4	0.44132754816644537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3262	0.19200094175814475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2810	0.16539627416933989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.9429942023014216E-5	0.0
22-23	0.0	0.0	0.0	8.828982606904265E-5	0.0
24-25	0.0	0.0	0.0	1.765796521380853E-4	0.0
26-27	0.0	0.0	0.0	3.237293622531564E-4	0.0
28-29	0.0	0.0	0.0	0.0010006180287824833	0.0
30-31	0.0	0.0	0.0	0.0037964625209688336	0.0
32-33	0.0	0.0	0.0	0.009770740751640718	0.0
34-35	0.0	0.0	0.0	0.018275993996291828	0.0
36-37	0.0	0.0	0.0	0.033667853674328264	0.0
38-39	0.0	0.0	0.0	0.06247976691485918	0.0
40-41	0.0	0.0	0.0	0.09982636334206421	0.0
42-43	0.0	0.0	0.0	0.13737896936343036	0.0
44-45	0.0	0.0	0.0	0.17775684981900586	0.0
46-47	0.0	0.0	0.0	0.2287883692869125	0.0
48-49	0.0	0.0	0.0	0.2864416257099973	0.0
50-51	0.0	0.0	0.0	0.3568380470290473	0.0
52-53	0.0	0.0	0.0	0.42229023808823096	0.0
54-55	0.0	0.0	0.0	0.4939815768562936	0.0
56-57	0.0	0.0	0.0	0.5763854145207334	0.0
58-59	0.0	0.0	0.0	0.6753288796021072	0.0
60-61	0.0	0.0	0.0	0.7817475499573265	0.0
62-63	0.0	0.0	0.0	0.8875776214720856	0.0
64-65	0.0	0.0	0.0	1.0034727331587157	0.0
66-67	0.0	0.0	0.0	1.1340828158568528	0.0
68-69	0.0	0.0	0.0	1.2758174166396894	0.0
70-71	0.0	0.0	0.0	1.4338856352452987	0.0
72-73	0.0	0.0	0.0	1.6058742164277935	0.0
74-75	0.0	0.0	0.0	1.7772741987698284	0.0
76-77	0.0	0.0	0.0	1.9598281291385857	0.0
78-79	0.0	0.0	0.0	2.172959769269254	0.0
80-81	0.0	0.0	0.0	2.4103122516848643	0.0
82-83	0.0	0.0	0.0	2.660643338532623	0.0
84-85	0.0	0.0	0.0	2.90714853291739	0.0
86-87	0.0	0.0	0.0	3.16510197474911	0.0
88	0.0	0.0	0.0	3.3784984843579857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2930	0.0	42.557747	1
GTATCAA	5165	0.0	38.080826	1
ATCAACG	5830	0.0	32.977688	3
TCAACGC	5830	0.0	32.977688	4
CAACGCA	5930	0.0	32.42157	5
AACGCAG	6125	0.0	31.379213	6
TATCAAC	6290	0.0	30.790167	2
ACGCAGA	7150	0.0	26.815073	7
CGCAGAG	7300	0.0	26.199703	8
GTACATG	4580	0.0	24.14364	1
GCAGAGT	7905	0.0	23.897308	9
TACATGG	4625	0.0	22.970062	2
GAGTACT	5195	0.0	21.076342	12-13
ACATGGG	4920	0.0	20.63736	3
GGACCGT	160	5.4218304E-5	20.559042	6
AGAGTAC	7020	0.0	20.550676	10-11
TATACCG	120	0.0050605526	19.58638	5
CAGAGTA	7790	0.0	19.394043	10-11
AGTACTT	5590	0.0	19.124691	12-13
CATGGGG	2835	0.0	18.7366	4
>>END_MODULE