FastQCFastQC Report
Wed 25 May 2016
SRR1033105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589347
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.037.508181
GTATCAA32250.034.329071
TCAACGC31550.033.652284
ATCAACG32250.032.927573
CAACGCA33900.031.5966175
AACGCAG37200.028.9088086
GTACATG75000.027.701371
TACATGG73850.027.0512472
TATCAAC40800.026.613422
ACATGGG76600.025.5179353
ACGCAGA43350.024.4830427
CGCAGAG44150.023.6139328
CATGGGG46400.021.6671224
CTGTCGC7350.018.52929
GAGTACA57200.017.9549581
GCAGAGT55800.017.842179
GTATAGA11650.016.9842851
TATACTG15150.016.7450545
AGTACAT57550.016.2538642
CTACGCT1750.002336567316.1071474
Produced by FastQC (version 0.11.5)