##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033105_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2589347
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673785321163983	33.0	31.0	34.0	30.0	34.0
2	31.72099915538551	34.0	31.0	34.0	30.0	34.0
3	31.89771745540478	34.0	31.0	34.0	30.0	34.0
4	35.273642736952596	37.0	35.0	37.0	33.0	37.0
5	35.337415186145385	37.0	35.0	37.0	33.0	37.0
6	35.42182218142258	37.0	36.0	37.0	35.0	37.0
7	35.317718328211704	37.0	36.0	37.0	33.0	37.0
8	35.32207695608198	37.0	36.0	37.0	33.0	37.0
9	36.98686541433033	39.0	38.0	39.0	34.0	39.0
10-11	37.002560491119965	39.0	38.0	39.0	34.5	39.0
12-13	36.975627252739784	39.0	38.0	39.0	34.0	39.0
14-15	38.43142749890223	41.0	39.0	41.0	34.5	41.0
16-17	38.4378177200661	41.0	39.0	41.0	34.5	41.0
18-19	38.42027642490558	41.0	39.0	41.0	34.0	41.0
20-21	38.46003393905877	41.0	39.0	41.0	34.0	41.0
22-23	38.418522121600546	41.0	39.0	41.0	34.0	41.0
24-25	38.41557948780137	41.0	39.0	41.0	34.0	41.0
26-27	38.38594363752715	40.0	39.0	41.0	34.0	41.0
28-29	38.36341710863781	40.0	39.0	41.0	34.0	41.0
30-31	38.36473211199581	40.0	39.0	41.0	34.0	41.0
32-33	38.436423159970445	41.0	39.0	41.0	34.0	41.0
34-35	38.40967896539166	41.0	39.0	41.0	34.0	41.0
36-37	38.32908393506162	41.0	39.0	41.0	34.0	41.0
38-39	38.247128716236176	41.0	39.0	41.0	33.0	41.0
40-41	38.18576285835772	41.0	38.0	41.0	33.0	41.0
42-43	38.0633613416819	40.0	38.0	41.0	33.0	41.0
44-45	37.930598332320855	40.0	38.0	41.0	33.0	41.0
46-47	37.80501280824856	40.0	38.0	41.0	33.0	41.0
48-49	37.681007991590164	40.0	38.0	41.0	32.5	41.0
50-51	37.55912089032486	40.0	37.5	41.0	32.0	41.0
52-53	37.412441051740075	40.0	37.0	41.0	32.0	41.0
54-55	37.26141726079973	40.0	37.0	41.0	31.5	41.0
56-57	37.10635210344539	40.0	36.5	41.0	31.0	41.0
58-59	36.90693000976694	40.0	36.0	41.0	31.0	41.0
60-61	36.68447585433702	39.0	35.5	41.0	31.0	41.0
62-63	36.41872738570767	39.0	35.0	41.0	31.0	41.0
64-65	36.15248033577578	39.0	35.0	41.0	30.0	41.0
66-67	35.85888893995281	38.0	35.0	40.0	30.0	41.0
68-69	35.549793442130394	37.5	35.0	40.0	30.0	41.0
70-71	35.20418178791796	37.0	35.0	40.0	29.5	41.0
72-73	34.837522935319214	36.5	34.0	39.0	29.0	41.0
74-75	34.46962187763942	36.0	34.0	39.0	29.0	41.0
76-77	34.13866198697973	35.5	34.0	38.5	29.0	40.0
78-79	33.77206975349384	35.0	34.0	37.0	29.0	39.5
80-81	33.41331038288804	35.0	34.0	37.0	28.0	39.0
82-83	33.189341752959336	35.0	34.0	36.5	28.0	39.0
84-85	32.917373762574115	35.0	34.0	36.0	28.0	37.5
86-87	32.661720310178595	35.0	34.0	36.0	28.0	37.0
88-89	32.3015945719133	35.0	34.0	35.5	27.0	37.0
90-91	32.11157562118943	35.0	33.5	35.0	27.0	36.0
92-93	31.931529262010848	35.0	33.0	35.0	26.0	36.0
94-95	31.75922674712968	35.0	33.0	35.0	26.0	36.0
96-97	31.578188632114582	35.0	33.0	35.0	25.0	36.0
98-99	31.383263425102932	35.0	33.0	35.0	25.0	35.0
100	31.216239461145996	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1795960385090929
1101	2	0.25621811383937043
1101	3	0.16321397451633857
1101	4	0.13570566405237372
1101	5	0.208502468943486
1101	6	0.11979622919460553
1101	7	0.09305811582133572
1101	8	0.06282323048922223
1101	9	0.15828611041413865
1101	10-11	0.1327461631402258
1101	12-13	0.18563200402981295
1101	14-15	0.238529115436954
1101	16-17	0.22352781308828185
1101	18-19	0.1624569543310983
1101	20-21	0.14877281685121346
1101	22-23	0.15776674764848053
1101	24-25	0.23165104861915609
1101	26-27	0.1892191427071097
1101	28-29	0.20881836369397888
1101	30-31	0.14167977813798416
1101	32-33	0.19785714595326453
1101	34-35	0.1655360085784494
1101	36-37	0.1657900086005455
1101	38-39	0.1386135271892428
1101	40-41	0.1267950200283181
1101	42-43	0.12561303675761337
1101	44-45	0.12636364138815992
1101	46-47	0.10934408998060263
1101	48-49	0.05187917104368722
1101	50-51	0.02037851372230648
1101	52-53	0.0337001242835413
1101	54-55	-0.05055271527148619
1101	56-57	-0.04739959097831559
1101	58-59	0.011365343410041362
1101	60-61	-0.04743836739294238
1101	62-63	-0.03703876058997935
1101	64-65	0.04768336084555358
1101	66-67	0.010102962388366166
1101	68-69	0.09916412748381731
1101	70-71	0.09851291652863381
1101	72-73	0.10462237802575203
1101	74-75	0.05381587222993289
1101	76-77	0.01945983232991466
1101	78-79	-0.005837415271194857
1101	80-81	-0.01067023079161089
1101	82-83	-0.06846364122782944
1101	84-85	-0.11275679022714513
1101	86-87	-0.007202504749585614
1101	88-89	0.1809619337364694
1101	90-91	0.12219062699573868
1101	92-93	0.18792423913786038
1101	94-95	0.126778773765448
1101	96-97	0.10666545703778851
1101	98-99	0.14156524930659486
1101	100	0.11532853826771472
1102	1	0.12940267417229734
1102	2	0.11083573049874573
1102	3	0.1942220083157551
1102	4	0.16156158735515902
1102	5	0.040602140987083146
1102	6	0.09837773130885807
1102	7	-0.023200403475158282
1102	8	0.006472785643950374
1102	9	0.04831103796212233
1102	10-11	0.024560634708393536
1102	12-13	0.03395902103324033
1102	14-15	0.07241430804428717
1102	16-17	0.0834239065802933
1102	18-19	0.14608387083752206
1102	20-21	0.12356601118951716
1102	22-23	0.09518411777428781
1102	24-25	0.07962847196187539
1102	26-27	0.11991391316743005
1102	28-29	0.09662472755034202
1102	30-31	0.051628564007842215
1102	32-33	0.0657320516255595
1102	34-35	0.08400379291164484
1102	36-37	0.0587224049950521
1102	38-39	0.0405913987748292
1102	40-41	0.10124651860193268
1102	42-43	0.11677573818376885
1102	44-45	0.1034014773765719
1102	46-47	0.050044898605939636
1102	48-49	0.10219130185429748
1102	50-51	0.05241178383272427
1102	52-53	-0.08951673942321747
1102	54-55	0.013449002595400827
1102	56-57	-0.05442022445514283
1102	58-59	0.0162176318924665
1102	60-61	0.035397746198469804
1102	62-63	0.15459623501701003
1102	64-65	0.14212684827031552
1102	66-67	0.05816866334601656
1102	68-69	0.06472974797232922
1102	70-71	0.0712760535610002
1102	72-73	0.09915032823265335
1102	74-75	0.0754145315546424
1102	76-77	0.07069095039779683
1102	78-79	0.04649851413531536
1102	80-81	0.054367452603493405
1102	82-83	0.06658680397507766
1102	84-85	0.10269811070045165
1102	86-87	0.10079094089083185
1102	88-89	0.1720540011493341
1102	90-91	0.16774941654929165
1102	92-93	0.2007051211033577
1102	94-95	0.2612440295797889
1102	96-97	0.2652687692663527
1102	98-99	0.3143275518442934
1102	100	0.32301041936176134
1103	1	0.06690726440244887
1103	2	0.05461430468145423
1103	3	0.0626226918010282
1103	4	0.04547294542065572
1103	5	0.04099776144849443
1103	6	0.04143363113167453
1103	7	-0.04421160822696635
1103	8	-0.030256514713165927
1103	9	0.02327764403774779
1103	10-11	0.0390103923442382
1103	12-13	0.08632594452328135
1103	14-15	0.08378014824001667
1103	16-17	0.06695602556823843
1103	18-19	0.029719528204388723
1103	20-21	0.028620973457826437
1103	22-23	0.05242468944155121
1103	24-25	0.04847242823534259
1103	26-27	0.08566907488123121
1103	28-29	0.12509935964339292
1103	30-31	0.1477581680086928
1103	32-33	0.14939129129984252
1103	34-35	0.10377577896652213
1103	36-37	0.19990956474423882
1103	38-39	0.13971569761717006
1103	40-41	0.14729455995072982
1103	42-43	0.132184853921828
1103	44-45	-0.03291688262599024
1103	46-47	0.009904483465518865
1103	48-49	0.08540110061860418
1103	50-51	0.061913560251802835
1103	52-53	-0.04400274266391335
1103	54-55	0.006467262530954088
1103	56-57	0.029945767218180208
1103	58-59	0.007247174613745244
1103	60-61	0.0738833833683259
1103	62-63	0.10207453470395933
1103	64-65	0.0769503137563774
1103	66-67	0.136437394927718
1103	68-69	0.1065922166440103
1103	70-71	0.03630179901437458
1103	72-73	0.04928292850622995
1103	74-75	0.06207503583732432
1103	76-77	0.08972749551516301
1103	78-79	0.10234870508889315
1103	80-81	0.09553980886042979
1103	82-83	0.07516594413316824
1103	84-85	0.09724491727732953
1103	86-87	0.09029451897524154
1103	88-89	0.1471205881755111
1103	90-91	0.16199326793600477
1103	92-93	0.11946679750130684
1103	94-95	0.13077362279359406
1103	96-97	0.1314594003770999
1103	98-99	0.22085067053846785
1103	100	0.2711267610401329
1104	1	0.01722076872080791
1104	2	0.02798486998163341
1104	3	-0.049133832273458466
1104	4	-0.034073694198802684
1104	5	-0.0792369476818493
1104	6	-0.131578813725433
1104	7	-0.06325468977004789
1104	8	-0.09726326193835888
1104	9	-0.05249029156028229
1104	10-11	-0.08990813129516084
1104	12-13	-0.05072513988822891
1104	14-15	-0.02867894313290975
1104	16-17	-0.036276302633744706
1104	18-19	-0.11843495095737921
1104	20-21	-0.14969897503808482
1104	22-23	-0.12061151423785788
1104	24-25	-0.07111143634244854
1104	26-27	-0.028800370292096034
1104	28-29	0.05564447253074434
1104	30-31	0.020352044091637822
1104	32-33	0.017833295664175353
1104	34-35	-0.06246971176318539
1104	36-37	-0.028963095257061866
1104	38-39	-0.05541936462954311
1104	40-41	-0.03450346286671646
1104	42-43	-0.03971625274868984
1104	44-45	-0.0935639748264876
1104	46-47	-0.08785010458032616
1104	48-49	-0.10059718762482106
1104	50-51	-0.10656886915295871
1104	52-53	-0.15853165497802735
1104	54-55	-0.142902981499482
1104	56-57	-0.18516194925483376
1104	58-59	-0.18443087386042123
1104	60-61	-0.19157877408413526
1104	62-63	-0.222329616459902
1104	64-65	-0.13313321777739873
1104	66-67	-0.10153591024825914
1104	68-69	-0.15087930220494172
1104	70-71	-0.09521691396671628
1104	72-73	-0.058468516927256076
1104	74-75	-0.0709846715549105
1104	76-77	-0.11697979523338375
1104	78-79	-0.08132920826135148
1104	80-81	-0.08654058650879648
1104	82-83	-0.12130395743381683
1104	84-85	-0.08141049317419657
1104	86-87	-0.009120873826077514
1104	88-89	0.00884210478173486
1104	90-91	-0.010149281633736962
1104	92-93	-0.08986948753279123
1104	94-95	-0.0633212375634038
1104	96-97	-0.015286965574365041
1104	98-99	0.00151546060859431
1104	100	0.011649093492078322
1105	1	5.128613144975702E-4
1105	2	0.019927777051378825
1105	3	0.037657401714771055
1105	4	0.04850821660108551
1105	5	0.0020918868571371263
1105	6	0.006772951309699238
1105	7	-0.012591122977447355
1105	8	-0.02329730009266484
1105	9	-0.09316303893519517
1105	10-11	-0.015542022087203122
1105	12-13	-0.03822171883486192
1105	14-15	0.05000998694969638
1105	16-17	-9.123180057528657E-4
1105	18-19	-0.09345266250812756
1105	20-21	-0.08378560517858347
1105	22-23	0.04415215999966904
1105	24-25	0.043325637302260134
1105	26-27	0.06861198322737039
1105	28-29	0.05734979290189557
1105	30-31	0.08231828754864523
1105	32-33	0.06586102228570212
1105	34-35	0.024298077008985786
1105	36-37	0.014611551825261415
1105	38-39	0.006299158958690043
1105	40-41	0.024460852439595726
1105	42-43	-0.06412678694626095
1105	44-45	0.005367413722815684
1105	46-47	0.00287387966072572
1105	48-49	-0.004388030501168316
1105	50-51	0.0028673197539461626
1105	52-53	-0.03740970532491161
1105	54-55	-0.12064487264569124
1105	56-57	-0.07112962468382733
1105	58-59	0.012357655530671252
1105	60-61	-0.07803772419794797
1105	62-63	-0.062476304242046865
1105	64-65	-0.06940428370709384
1105	66-67	-0.10016543996140825
1105	68-69	-0.24135805217319728
1105	70-71	-0.2486130509206319
1105	72-73	-0.1542168647113087
1105	74-75	-0.18214613277900327
1105	76-77	-0.13755720455178988
1105	78-79	-0.1860057495961982
1105	80-81	-0.21970867473482514
1105	82-83	-0.21473814819027837
1105	84-85	-0.23965787527062332
1105	86-87	-0.17370145369008583
1105	88-89	-0.17063451738201962
1105	90-91	-0.12998849581995842
1105	92-93	-0.14789246733779393
1105	94-95	-0.14193798459543316
1105	96-97	-0.1490908625320344
1105	98-99	-0.14794708636413034
1105	100	-0.08131548757146945
1106	1	0.1623421657584032
1106	2	0.10899183504582766
1106	3	0.07875476878836807
1106	4	0.06034425831828116
1106	5	0.0674191835936
1106	6	0.04583566184949461
1106	7	0.024329134655886264
1106	8	0.004056029467797373
1106	9	0.03035543466816648
1106	10-11	-0.05484323210149
1106	12-13	-0.006687237470160312
1106	14-15	0.05179132200776593
1106	16-17	0.052328310447101956
1106	18-19	0.09833055898646847
1106	20-21	0.09033113829299566
1106	22-23	0.06079457248247877
1106	24-25	0.07227974299660644
1106	26-27	0.08816489777179726
1106	28-29	0.10157460223705783
1106	30-31	0.07666436780304764
1106	32-33	0.073734199853277
1106	34-35	0.04653512123245207
1106	36-37	0.09998627896569445
1106	38-39	0.08143457815183552
1106	40-41	0.03221680145191641
1106	42-43	0.06351759282036085
1106	44-45	0.05390273050940664
1106	46-47	0.09459377078638909
1106	48-49	0.09421600647299044
1106	50-51	0.1596531014123883
1106	52-53	0.1336483160726658
1106	54-55	0.15884821491190593
1106	56-57	0.1509861486204045
1106	58-59	0.11357710199630588
1106	60-61	0.1663501271508494
1106	62-63	0.17071689380684063
1106	64-65	0.09577883538094767
1106	66-67	0.15318298486409532
1106	68-69	0.08916583819789281
1106	70-71	0.15943394829784552
1106	72-73	0.21002573102534683
1106	74-75	0.20802283803512722
1106	76-77	0.22092367506661503
1106	78-79	0.23436102756173938
1106	80-81	0.24077991232422136
1106	82-83	0.16639883405026268
1106	84-85	0.11575754500048419
1106	86-87	0.1706351943817026
1106	88-89	0.3316494182635523
1106	90-91	0.26770466180528985
1106	92-93	0.30241475232426396
1106	94-95	0.3264695934895663
1106	96-97	0.2608290986612687
1106	98-99	0.2344403337991814
1106	100	0.2690361980943088
1107	1	0.14485615736437296
1107	2	0.11169983363500435
1107	3	0.1366284179149062
1107	4	0.16114036623513073
1107	5	0.12755630972986154
1107	6	0.1032101106296679
1107	7	0.09087114119867579
1107	8	0.09424986849575134
1107	9	0.11084351264419467
1107	10-11	0.06635720010336854
1107	12-13	0.07092647290406262
1107	14-15	0.006099840885006813
1107	16-17	0.017557653350159796
1107	18-19	0.09090553701232551
1107	20-21	0.04253303170858658
1107	22-23	0.013428764067185739
1107	24-25	0.08977106997376438
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2215	88-89	-0.14358482423652674
2215	90-91	-0.1291756133675861
2215	92-93	-0.10033975676939022
2215	94-95	-0.1524640718941832
2215	96-97	-0.16525215042022978
2215	98-99	-0.2816637139588849
2215	100	-0.22289092557858226
2216	1	0.21395557427970857
2216	2	0.1983088776523445
2216	3	0.14877356578085
2216	4	0.2744106743583785
2216	5	0.19708710339309476
2216	6	0.2667786443056315
2216	7	0.2935960519491161
2216	8	0.31749587909185806
2216	9	0.3346630787784477
2216	10-11	0.30765676789851426
2216	12-13	0.24780837656493304
2216	14-15	0.30618511900025425
2216	16-17	0.3013179721012378
2216	18-19	0.2734167118686699
2216	20-21	0.3026744966889865
2216	22-23	0.2770618030589276
2216	24-25	0.20236276304673595
2216	26-27	0.21416740403746104
2216	28-29	0.26192078018192433
2216	30-31	0.22850490665016565
2216	32-33	0.23510638030440134
2216	34-35	0.1627161989266881
2216	36-37	0.17873784538173254
2216	38-39	0.18166954055785567
2216	40-41	0.23767576135166024
2216	42-43	0.21639979833414458
2216	44-45	0.2206477179780748
2216	46-47	0.28817929710217527
2216	48-49	0.36343593128501794
2216	50-51	0.3433623745953511
2216	52-53	0.3927100328646489
2216	54-55	0.3425809843354628
2216	56-57	0.23825901829458473
2216	58-59	0.2560974654048209
2216	60-61	0.412394926649597
2216	62-63	0.37655179355621726
2216	64-65	0.48356079026816445
2216	66-67	0.38152728310971185
2216	68-69	0.431388582558796
2216	70-71	0.40245556483919387
2216	72-73	0.45964979117572113
2216	74-75	0.35495266259653135
2216	76-77	0.2941066324600925
2216	78-79	0.32954429823843867
2216	80-81	0.4119970175873746
2216	82-83	0.43054357089237527
2216	84-85	0.32690259512579445
2216	86-87	0.2534829387426072
2216	88-89	0.3182174007196892
2216	90-91	0.26106305779526195
2216	92-93	0.292608987913237
2216	94-95	0.35699121003091605
2216	96-97	0.2869020936487381
2216	98-99	0.22903619846083245
2216	100	0.23308694997400536
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25227.0
3	4492.0
4	2984.0
5	2412.0
6	1961.0
7	2259.0
8	2768.0
9	3032.0
10	3328.0
11	3624.0
12	3995.0
13	4465.0
14	5030.0
15	5287.0
16	5254.0
17	5260.0
18	5192.0
19	5232.0
20	5289.0
21	5557.0
22	6289.0
23	8375.0
24	13396.0
25	18710.0
26	20110.0
27	17125.0
28	17544.0
29	21908.0
30	27135.0
31	32556.0
32	43455.0
33	59813.0
34	84819.0
35	131757.0
36	248901.0
37	528930.0
38	842878.0
39	362198.0
40	800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03626145569689	19.877077446943055	13.199876627976002	23.886784469384054
2	15.416491218708881	20.13666960916575	40.72407130514334	23.72276786698203
3	18.159295017581663	25.39770810379655	29.956149215559048	26.48684766306274
4	11.94128860319543	16.602416546318423	39.444551334803016	32.011743515683136
5	12.51802880668135	38.4379760754403	35.97663103469992	13.06736408317843
6	30.85471003185648	38.4774007458928	17.82730913147056	12.84058009078016
7	27.32269393294636	32.5698744120245	22.69443503563482	17.41299661939432
8	24.722465884963118	37.12717186547035	20.365776077739294	17.784586171827232
9	24.869423004572607	17.060837562294942	21.10924587874204	36.9604935543904
10-11	23.698004685541456	27.13361132410002	29.132981692395944	20.03540229796258
12-13	24.28341273526056	24.524708587073153	29.03491702002906	22.156961657637225
14-15	21.59417460014883	26.57488142990599	26.016413728077065	25.814530241868123
16-17	21.37286280835567	29.865253067671283	26.59811120986785	22.163772914105202
18-19	21.26650995643054	28.934128974803336	28.469444690692352	21.329916378073776
20-21	22.483682171935072	28.2159264783197	27.917363436090163	21.38302791365506
22-23	22.04625032107549	28.00937057615911	27.989381816215424	21.95499728654998
24-25	21.975786786510117	28.155658966114594	27.900783034642153	21.967771212733133
26-27	21.705289964921036	28.094033949515108	28.031864052067434	22.168812033496422
28-29	21.74685244373663	28.072055278096826	28.14496343679983	22.03612884136672
30-31	21.942583044530934	28.571486512168327	27.726865035158443	21.7590654081423
32-33	21.607299547245276	28.196144911642236	28.2446633137721	21.951892227340387
34-35	21.762331879269226	28.007140345093507	28.26751612284592	21.96301165279135
36-37	22.130021764277615	28.322567064680655	27.690225000531072	21.85718617051066
38-39	21.87369821716707	28.54931300628911	27.829343211030523	21.74764556551329
40-41	21.603760376423878	28.190888541492658	28.183974948848107	22.02137613323536
42-43	21.643099185419942	28.031279287156995	28.179147746990246	22.146473780432814
44-45	21.74914642813002	28.243437924854774	28.11488073167717	21.89253491533803
46-47	21.780422409877552	28.44131110513745	28.02053497217613	21.757731512808867
48-49	21.73944192472034	28.24148876204336	27.99587600755975	22.023193305676553
50-51	21.71608307936043	28.138832768741693	27.977044531739114	22.168039620158762
52-53	21.72857639708948	28.14086965287384	28.07349257548637	22.05706137455031
54-55	21.85813538404617	28.532832601823248	27.863959014065117	21.745073000065464
56-57	21.71679341007648	28.231927984794265	27.883820504219987	22.167458100909265
58-59	21.570443383618194	27.90656404102837	28.303276410849882	22.21971616450355
60-61	21.924352903918496	28.30561627646509	27.871264376694498	21.898766442921914
62-63	21.66634707981619	28.27032451679144	27.95015835480165	22.113170048590717
64-65	21.679237365786005	28.0649104956825	28.047087066582186	22.2087650719493
66-67	21.546291976531144	28.060957693650497	27.944092312065326	22.448658017753033
68-69	21.974281895773146	28.253108844960085	27.858344383764432	21.914264875502337
70-71	21.944576291020432	28.01548996655066	27.936027998419643	22.103905744009264
72-73	21.67485515898188	28.164242560116048	28.03003663725143	22.13086564365064
74-75	21.774375127689495	28.158077371819058	27.942052818360562	22.125494682130885
76-77	21.775326020544913	28.150424794168515	27.787887939924012	22.286361245362563
78-79	21.85913214855307	28.335219009781383	27.818352488446006	21.98729635321954
80-81	21.778836340730507	28.058848599085177	27.95630943192336	22.206005628260954
82-83	21.671475272194236	28.126277502079244	28.09138365218305	22.110863573543472
84-85	21.656425357200305	28.045797490681245	27.950172027350582	22.347605124767863
86-87	21.859010999196688	28.11026617438052	27.87684221096212	22.15388061546067
88-89	21.654692973952898	28.07320354785482	27.92156224345209	22.350541234740195
90-91	21.6384780764692	27.8749972314604	28.071592685460097	22.414932006610304
92-93	21.785287987653344	28.12357692493002	27.863829297822708	22.22730578959393
94-95	21.59968593463874	27.823125723464358	28.103605049987635	22.473583291909264
96-97	21.66364707027278	27.902177897253694	28.030537453193343	22.40363757928019
98-99	21.64664277733663	27.89931362204614	28.001660391314687	22.452383209302543
100	21.721173752529555	27.87578732349444	27.916151431838077	22.48688749213793
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	328.0
1	342.0
2	344.0
3	302.0
4	246.0
5	218.5
6	204.0
7	191.5
8	179.0
9	188.0
10	200.5
11	204.5
12	233.5
13	245.5
14	270.5
15	330.5
16	409.5
17	567.0
18	827.0
19	1186.0
20	1748.5
21	2703.5
22	4051.5
23	6190.5
24	8907.5
25	12106.0
26	16429.5
27	21953.5
28	29151.5
29	36856.5
30	43862.0
31	51315.0
32	58653.5
33	65141.0
34	71870.0
35	77563.0
36	81934.0
37	86680.5
38	90237.5
39	91636.0
40	92871.0
41	93575.0
42	92467.0
43	92124.5
44	91727.5
45	90180.0
46	89421.5
47	87276.5
48	84952.5
49	83510.5
50	81344.5
51	78259.5
52	72666.0
53	67377.5
54	63846.5
55	60491.0
56	56179.5
57	50737.0
58	45875.0
59	41872.5
60	37544.5
61	32508.5
62	27863.0
63	23528.0
64	19499.0
65	15739.0
66	12386.5
67	9390.0
68	7070.0
69	5406.0
70	4008.0
71	2974.0
72	2239.5
73	1782.5
74	1370.0
75	1013.5
76	691.5
77	520.0
78	385.0
79	270.0
80	196.0
81	107.0
82	65.0
83	44.5
84	31.5
85	28.5
86	20.5
87	11.5
88	10.0
89	10.5
90	7.0
91	4.5
92	5.0
93	4.5
94	2.5
95	2.0
96	1.5
97	2.0
98	1.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32961978444758466
2	0.12010750200726282
3	0.06596257666508197
4	0.039585269954162186
5	0.04356310683736093
6	0.009075647257783526
7	0.006410882743796022
8	0.014907233368104006
9	0.017494758330961434
10-11	0.0205457206005993
12-13	0.01579548820609984
14-15	0.018440942832304824
16-17	0.019078169129127923
18-19	0.01902023946578037
20-21	0.023712542196932276
22-23	0.015138952021494224
24-25	0.024581487147145593
26-27	0.01828646373004468
28-29	0.01828646373004468
30-31	0.01911678890469296
32-33	0.024658726698275665
34-35	0.015312741011536886
36-37	0.00934598568673878
38-39	0.013497611559980182
40-41	0.009442535125651371
42-43	0.008554280287655536
44-45	0.008766689053263235
46-47	0.007839814439702365
48-49	0.004190245648806436
50-51	0.006179164090405804
52-53	0.007241207918444303
54-55	0.002645454626204985
56-57	0.002336496421684695
58-59	0.0025295952995098765
60-61	0.0033599204741581565
62-63	0.0025682150750749124
64-65	0.0022592568705546226
66-67	0.0016606503492965603
68-69	0.0031475117085504566
70-71	0.002239946982772104
72-73	0.001641340461514042
74-75	0.002239946982772104
76-77	0.003591639127548374
78-79	0.0034950896886357836
80-81	0.003572329239765856
82-83	0.0025295952995098765
84-85	0.003031652381855348
86-87	0.002587524962857431
88-89	0.5989927190137128
90-91	0.6097290166207928
92-93	0.6061373774932445
94-95	0.5932190625667397
96-97	0.5919446099730936
98-99	0.5891446762446285
100	0.5900329310826243
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2589347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.88533624219531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40821448686313	53.11245126346205
2	12.773130893768359	17.086703093905385
3	3.7666525009299137	7.558014570966096
4	1.4962341442016331	4.003044953279184
5	0.7576986248842512	2.533946364781608
6	0.42918476383581733	1.7223700343511499
7	0.28919717092036085	1.3540135012109973
8	0.1857106121342642	0.9937053389075344
9	0.1456267493010304	0.876626468357157
>10	0.7052548640326461	8.267822884682225
>50	0.03375064258788834	1.5413964104120397
>100	0.009286811903310738	0.9296703283587738
>500	5.773463739043261E-5	0.02023478732572481
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.861977556503628E-5	0.0
6	0.0	0.0	0.0	3.861977556503628E-5	0.0
7	0.0	0.0	0.0	3.861977556503628E-5	0.0
8	0.0	0.0	0.0	3.861977556503628E-5	0.0
9	0.0	0.0	0.0	1.1585932669510885E-4	0.0
10-11	0.0	0.0	0.0	1.1585932669510885E-4	0.0
12-13	0.0	0.0	1.930988778251814E-5	1.1585932669510885E-4	0.0
14-15	0.0	0.0	3.861977556503628E-5	1.35169214477627E-4	0.0
16-17	0.0	0.0	3.861977556503628E-5	1.9309887782518141E-4	0.0
18-19	0.0	0.0	3.861977556503628E-5	1.9309887782518141E-4	0.0
20-21	0.0	0.0	3.861977556503628E-5	1.9309887782518141E-4	0.0
22-23	0.0	0.0	3.861977556503628E-5	2.896483167377721E-4	0.0
24-25	0.0	0.0	3.861977556503628E-5	4.4412741899791725E-4	0.0
26-27	0.0	0.0	3.861977556503628E-5	6.565361846056168E-4	0.0
28-29	0.0	0.0	3.861977556503628E-5	0.001641340461514042	0.0
30-31	0.0	0.0	3.861977556503628E-5	0.005406768579105079	0.0
32-33	0.0	0.0	3.861977556503628E-5	0.0128796951509396	0.0
34-35	0.0	0.0	3.861977556503628E-5	0.024060120177017603	0.0
36-37	0.0	0.0	3.861977556503628E-5	0.046903717423736564	0.0
38-39	0.0	0.0	3.861977556503628E-5	0.08299389768926296	0.0
40-41	0.0	0.0	3.861977556503628E-5	0.1266535539655365	0.0
42-43	1.930988778251814E-5	0.0	3.861977556503628E-5	0.17344141206257796	0.0
44-45	3.861977556503628E-5	0.0	3.861977556503628E-5	0.22519191131972655	0.0
46-47	3.861977556503628E-5	0.0	3.861977556503628E-5	0.28982210572781475	0.0
48-49	3.861977556503628E-5	0.0	3.861977556503628E-5	0.36034181590957104	0.0
50-51	3.861977556503628E-5	0.0	3.861977556503628E-5	0.43908753828668	0.0
52-53	3.861977556503628E-5	0.0	3.861977556503628E-5	0.5182966979705694	0.0
54-55	3.861977556503628E-5	0.0	3.861977556503628E-5	0.6048822347873808	0.0
56-57	5.7929663347554424E-5	0.0	3.861977556503628E-5	0.7020688999967946	0.0
58-59	7.723955113007257E-5	0.0	3.861977556503628E-5	0.8164413653326494	0.0
60-61	7.723955113007257E-5	0.0	3.861977556503628E-5	0.935679922389699	0.0
62-63	7.723955113007257E-5	0.0	3.861977556503628E-5	1.060112839260246	0.0
64-65	7.723955113007257E-5	0.0	3.861977556503628E-5	1.1906863004456336	0.0
66-67	7.723955113007257E-5	0.0	3.861977556503628E-5	1.3387352100742003	0.0
68-69	7.723955113007257E-5	0.0	3.861977556503628E-5	1.5088360115504025	0.0
70-71	7.723955113007257E-5	0.0	3.861977556503628E-5	1.6942109342625766	0.0
72-73	7.723955113007257E-5	0.0	3.861977556503628E-5	1.8898973370506154	0.0
74-75	7.723955113007257E-5	0.0	3.861977556503628E-5	2.0918787632557554	0.0
76-77	7.723955113007257E-5	0.0	3.861977556503628E-5	2.3055426715693184	0.0
78-79	7.723955113007257E-5	0.0	3.861977556503628E-5	2.5470321281774906	0.0
80-81	7.723955113007257E-5	0.0	3.861977556503628E-5	2.8126782544015922	0.0
82-83	7.723955113007257E-5	0.0	3.861977556503628E-5	3.090199961611943	0.0
84-85	7.723955113007257E-5	0.0	3.861977556503628E-5	3.3693823191715904	0.0
86-87	7.723955113007257E-5	0.0	3.861977556503628E-5	3.661096793902092	0.0
88	7.723955113007257E-5	0.0	3.861977556503628E-5	3.900481472741969	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2085	0.0	37.50818	1
GTATCAA	3225	0.0	34.32907	1
TCAACGC	3155	0.0	33.65228	4
ATCAACG	3225	0.0	32.92757	3
CAACGCA	3390	0.0	31.596617	5
AACGCAG	3720	0.0	28.908808	6
GTACATG	7500	0.0	27.70137	1
TACATGG	7385	0.0	27.051247	2
TATCAAC	4080	0.0	26.61342	2
ACATGGG	7660	0.0	25.517935	3
ACGCAGA	4335	0.0	24.483042	7
CGCAGAG	4415	0.0	23.613932	8
CATGGGG	4640	0.0	21.667122	4
CTGTCGC	735	0.0	18.5292	9
GAGTACA	5720	0.0	17.954958	1
GCAGAGT	5580	0.0	17.84217	9
GTATAGA	1165	0.0	16.984285	1
TATACTG	1515	0.0	16.745054	5
AGTACAT	5755	0.0	16.253864	2
CTACGCT	175	0.0023365673	16.107147	4
>>END_MODULE