FastQCFastQC Report
Wed 25 May 2016
SRR1033105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589347
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51500.19889184415993683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.16386370772244896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35050.054.0950161
GTATCAA66500.044.0057031
TCAACGC78700.036.4363174
ATCAACG79900.035.947923
CAACGCA81350.035.2500725
AACGCAG85150.033.7204486
TATCAAC86400.033.406732
ACGCAGA99300.028.7260687
CGCAGAG101800.027.881598
GCAGAGT112600.024.8317289
GTACATG71700.023.6879921
TACATGG73550.021.7947082
GTGGTAT16450.021.1644061
AGAGTAC105600.020.8039610-11
ACATGGG75800.020.5275963
GAGTACT78350.020.4519812-13
CAGAGTA111100.020.36622810-11
TATACCG1857.17295E-620.328535
AGTACTT82500.018.99598712-13
GTACTTT84450.018.94686914-15
Produced by FastQC (version 0.11.5)