FastQCFastQC Report
Wed 25 May 2016
SRR1033104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631000
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC28550.036.034954
ATCAACG28850.035.5048333
CAACGCA29500.034.715925
GTATCAA31600.033.982721
AACGCAG31300.032.7101446
GGTATCA20800.029.4367851
GTACATG72050.028.2397021
TATCAAC37450.027.866632
TACATGG73300.026.6150552
ACGCAGA38000.026.448477
ACATGGG73750.025.4207083
CGCAGAG39000.025.2886148
GTATCGT1452.549691E-522.672556
CATGGGG49850.021.203294
GAGTACA51250.020.3099461
GCAGAGT50200.018.617439
AGTACAT52350.018.0493952
GTACGAC1851.6542262E-417.7788283
CTGTCGC7000.017.4439839
TCTGTCG7000.016.773068
Produced by FastQC (version 0.11.5)