##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2631000 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.282236031927024 33.0 31.0 34.0 29.0 34.0 2 31.412093500570126 33.0 31.0 34.0 28.0 34.0 3 31.61796883314329 34.0 31.0 34.0 29.0 34.0 4 34.97568263017864 37.0 35.0 37.0 33.0 37.0 5 35.087055112124666 37.0 35.0 37.0 33.0 37.0 6 35.1573842645382 37.0 35.0 37.0 33.0 37.0 7 35.06559064994299 37.0 35.0 37.0 33.0 37.0 8 35.083118586088936 37.0 35.0 37.0 33.0 37.0 9 36.71488445458001 39.0 38.0 39.0 33.0 39.0 10-11 36.69981736982136 39.0 38.0 39.0 33.0 39.0 12-13 36.64491904218929 39.0 38.0 39.0 33.0 39.0 14-15 38.05249144811859 41.0 39.0 41.0 33.0 41.0 16-17 38.04997966552642 41.0 38.5 41.0 33.0 41.0 18-19 38.0307727099962 41.0 38.5 41.0 33.0 41.0 20-21 38.050839224629414 40.5 39.0 41.0 33.0 41.0 22-23 38.01046883314329 40.0 38.5 41.0 33.0 41.0 24-25 37.997854808057774 40.0 38.0 41.0 33.0 41.0 26-27 37.949679969593305 40.0 38.0 41.0 33.0 41.0 28-29 37.91376054732041 40.0 38.0 41.0 32.5 41.0 30-31 37.911476814899274 40.0 38.0 41.0 33.0 41.0 32-33 37.97753496769289 40.0 38.0 41.0 33.0 41.0 34-35 37.9409606613455 40.0 38.0 41.0 33.0 41.0 36-37 37.86141391106044 40.0 38.0 41.0 32.5 41.0 38-39 37.77489452679589 40.0 38.0 41.0 32.0 41.0 40-41 37.70662979855568 40.0 38.0 41.0 32.0 41.0 42-43 37.58244450779171 40.0 38.0 41.0 32.0 41.0 44-45 37.45128905359179 40.0 38.0 41.0 31.5 41.0 46-47 37.318823071075634 40.0 38.0 41.0 31.0 41.0 48-49 37.19086354998099 40.0 37.0 41.0 31.0 41.0 50-51 37.06359958190802 40.0 37.0 41.0 31.0 41.0 52-53 36.90603534777651 40.0 37.0 41.0 30.0 41.0 54-55 36.743536107943754 40.0 36.0 41.0 30.0 41.0 56-57 36.58114861269479 40.0 36.0 41.0 30.0 41.0 58-59 36.37833599391867 39.5 35.0 41.0 30.0 41.0 60-61 36.14901254275941 39.0 35.0 41.0 30.0 41.0 62-63 35.876825921702775 39.0 35.0 41.0 29.0 41.0 64-65 35.603591790193846 38.5 35.0 40.5 29.0 41.0 66-67 35.31396313188901 38.0 35.0 40.0 29.0 41.0 68-69 35.001068034967695 37.0 34.5 40.0 29.0 41.0 70-71 34.657370581527935 37.0 34.0 39.5 28.0 41.0 72-73 34.29044355758267 36.0 34.0 39.0 28.0 41.0 74-75 33.919037438236415 36.0 34.0 39.0 27.5 40.5 76-77 33.59166343595591 35.0 34.0 38.5 27.0 40.0 78-79 33.22764500190041 35.0 34.0 37.0 27.0 39.0 80-81 32.8687107563664 35.0 33.0 37.0 26.0 39.0 82-83 32.651494108703915 35.0 33.0 36.0 26.0 39.0 84-85 32.37776339794755 35.0 33.0 36.0 26.0 37.0 86-87 32.12060927404029 35.0 33.0 36.0 26.0 37.0 88-89 31.76203534777651 35.0 33.0 35.0 25.0 37.0 90-91 31.56671854808058 35.0 33.0 35.0 25.0 36.0 92-93 31.382089889775752 35.0 33.0 35.0 24.0 36.0 94-95 31.199577537058154 35.0 33.0 35.0 23.5 36.0 96-97 31.012193272519955 35.0 33.0 35.0 22.0 36.0 98-99 30.79911383504371 35.0 33.0 35.0 19.5 35.0 100 30.624969973394148 35.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1894844732658285 1101 2 0.17714521025350294 1101 3 0.1456401158944729 1101 4 0.07137548106690872 1101 5 0.12040924473726733 1101 6 0.1972851379476097 1101 7 0.15262104155155498 1101 8 0.13237677284889315 1101 9 0.1749519252809293 1101 10-11 0.17368632247208637 1101 12-13 0.19279191072367752 1101 14-15 0.26308205088948 1101 16-17 0.15599099843115027 1101 18-19 0.1112184670565668 1101 20-21 0.1375147600623876 1101 22-23 0.1771607428192823 1101 24-25 0.1231990972228445 1101 26-27 0.1773256452258707 1101 28-29 0.2098052371965906 1101 30-31 0.24291057298504626 1101 32-33 0.257164581643039 1101 34-35 0.2870753096034093 1101 36-37 0.2915730707085231 1101 38-39 0.2508968197601149 1101 40-41 0.29097203779124925 1101 42-43 0.3484739965591217 1101 44-45 0.2061506269613389 1101 46-47 0.2409939844160931 1101 48-49 0.24569043984514138 1101 50-51 0.22868825192447417 1101 52-53 0.17954767541428396 1101 54-55 0.19407018082043948 1101 56-57 0.11139994539470166 1101 58-59 0.08598937515381522 1101 60-61 0.09334734325995697 1101 62-63 0.053562883236345726 1101 64-65 -0.002870181280137274 1101 66-67 0.08877828464473225 1101 68-69 0.06912330155198987 1101 70-71 0.1191821493496974 1101 72-73 0.14602354197479883 1101 74-75 0.08599250246322043 1101 76-77 0.06825032020770294 1101 78-79 0.09531058442289009 1101 80-81 0.1573725198878293 1101 82-83 0.13389048761318634 1101 84-85 0.12262953988619074 1101 86-87 0.09698003938041211 1101 88-89 0.2939222917377009 1101 90-91 0.41667780867697957 1101 92-93 0.4773498284150399 1101 94-95 0.44656500793947984 1101 96-97 0.39413441255879533 1101 98-99 0.4078724361387991 1101 100 0.43305801272431665 1102 1 0.01113250061672133 1102 2 0.03039739806976982 1102 3 0.12075223244193367 1102 4 0.02841131107634709 1102 5 -0.05440731888162276 1102 6 0.06533558741734424 1102 7 -0.06514438372250453 1102 8 -0.0038123701394709997 1102 9 0.013068387694715966 1102 10-11 0.018264127086695225 1102 12-13 0.0789556588845528 1102 14-15 0.044920718043741203 1102 16-17 0.04973519614680555 1102 18-19 -0.03783784210377661 1102 20-21 0.04198744744066829 1102 22-23 0.061778485179992515 1102 24-25 0.04576678132684009 1102 26-27 0.19091550090229248 1102 28-29 0.10431577018179894 1102 30-31 0.10563110231326789 1102 32-33 0.14254270603176877 1102 34-35 0.16015560182252386 1102 36-37 0.12105699270405523 1102 38-39 0.12368152503213992 1102 40-41 0.19489879591105108 1102 42-43 0.16766190637601142 1102 44-45 0.07492243921310404 1102 46-47 0.12381258914636817 1102 48-49 0.18664088336163331 1102 50-51 0.17268206055327084 1102 52-53 0.09409712558990435 1102 54-55 0.17850935995347328 1102 56-57 0.0780264135065849 1102 58-59 0.11112470934328655 1102 60-61 0.09056367555288602 1102 62-63 0.045668680843142795 1102 64-65 0.08194820232973399 1102 66-67 -0.013082868753286903 1102 68-69 -0.0429483754761506 1102 70-71 0.057196488157174485 1102 72-73 0.05937224034105526 1102 74-75 0.006401200999576417 1102 76-77 -0.057976294183823995 1102 78-79 -0.022938584249452276 1102 80-81 -0.0560893240862157 1102 82-83 -0.01393853528978184 1102 84-85 -0.053126343192147374 1102 86-87 -0.08606870065854366 1102 88-89 0.05627504681284279 1102 90-91 0.14146173152296626 1102 92-93 0.16722145951817424 1102 94-95 0.08977448550001199 1102 96-97 0.07132754421898468 1102 98-99 0.16312532133478896 1102 100 0.1631296727431959 1103 1 0.09344053773923022 1103 2 -0.01689481716108432 1103 3 0.13105376150407366 1103 4 0.08017124333939307 1103 5 0.0482316995966201 1103 6 0.05601717392308103 1103 7 0.058153675480916434 1103 8 0.040514294645618065 1103 9 0.09058157813849022 1103 10-11 0.1287336039515452 1103 12-13 0.17487663243725393 1103 14-15 0.16307046984093176 1103 16-17 0.12387936960653434 1103 18-19 0.13952154560728047 1103 20-21 0.10055232051688989 1103 22-23 0.15245705008627652 1103 24-25 0.14373507113474915 1103 26-27 0.18682329231231876 1103 28-29 0.18224926178939427 1103 30-31 0.20109508385162655 1103 32-33 0.21944048631832658 1103 34-35 0.16504660841358998 1103 36-37 0.2640831079564876 1103 38-39 0.21585950056049796 1103 40-41 0.26066971882705303 1103 42-43 0.11269748487949727 1103 44-45 0.09109138314749288 1103 46-47 0.14344949914587346 1103 48-49 0.13877134340845743 1103 50-51 0.06164950364924948 1103 52-53 0.024407453815300073 1103 54-55 0.07778115448471112 1103 56-57 0.12664859735928502 1103 58-59 0.17772627327003931 1103 60-61 0.1429338380883749 1103 62-63 0.11899516300407953 1103 64-65 0.11902588399924952 1103 66-67 0.12788454113830028 1103 68-69 0.10684366543165424 1103 70-71 0.06614440707205915 1103 72-73 0.13047064154969235 1103 74-75 0.028520039510034678 1103 76-77 0.08362472187049264 1103 78-79 0.06927734389625329 1103 80-81 0.15148267133157844 1103 82-83 0.04511846004500342 1103 84-85 0.13355046188148378 1103 86-87 0.13334042722816264 1103 88-89 0.1367928028601888 1103 90-91 0.1776600281377796 1103 92-93 0.18238381238122514 1103 94-95 0.19548896095366786 1103 96-97 0.23023296151515282 1103 98-99 0.271701185063975 1103 100 0.30088113788688986 1104 1 0.05666469338151359 1104 2 0.025730687869952362 1104 3 0.03781250408879089 1104 4 0.0337184156865078 1104 5 -0.01847954037828714 1104 6 0.050791183603841716 1104 7 -0.014801821361267287 1104 8 0.11379237062062231 1104 9 0.04584009985352111 1104 10-11 0.07253809208604878 1104 12-13 0.09356657046872385 1104 14-15 0.019088113890681768 1104 16-17 0.07487937794123667 1104 18-19 0.05402199203492586 1104 20-21 0.04643625245568472 1104 22-23 0.09382259514473645 1104 24-25 0.06308292994901166 1104 26-27 0.15338608337584247 1104 28-29 0.17780940345853935 1104 30-31 0.051939610867528074 1104 32-33 0.006731324289575014 1104 34-35 0.010495976642943106 1104 36-37 0.03935061292084896 1104 38-39 -0.01689912012915329 1104 40-41 -0.02483788294490097 1104 42-43 -0.03113419523035077 1104 44-45 -0.12071834699559503 1104 46-47 -0.09767950958681126 1104 48-49 -0.2088923215489089 1104 50-51 -0.21466804392628802 1104 52-53 -0.18391361621497992 1104 54-55 -0.22673068183834744 1104 56-57 -0.14132564996474883 1104 58-59 -0.2156223195191984 1104 60-61 -0.13955445165098013 1104 62-63 -0.15027591233957338 1104 64-65 -0.2025692326970372 1104 66-67 -0.1719655904458648 1104 68-69 -0.2534384825677094 1104 70-71 -0.23761593838063533 1104 72-73 -0.12027889587416496 1104 74-75 -0.1112160446579864 1104 76-77 -0.18845938155485698 1104 78-79 -0.2262640707324053 1104 80-81 -0.19035718356120412 1104 82-83 -0.16058114989080252 1104 84-85 -0.18749313175163707 1104 86-87 -0.11634018426831005 1104 88-89 -0.053607544280275476 1104 90-91 -0.054093414095419234 1104 92-93 0.04043662211449117 1104 94-95 0.029588544530962935 1104 96-97 0.02541285831066631 1104 98-99 0.00864167891608858 1104 100 0.05485926657890161 1105 1 0.10287805682201423 1105 2 0.09247412211792749 1105 3 0.10756453969058555 1105 4 0.12498992158445077 1105 5 0.09558013676053889 1105 6 0.1382044964747564 1105 7 0.06494820100797938 1105 8 0.11033801796662601 1105 9 0.13510534789080708 1105 10-11 0.1642497883890428 1105 12-13 0.17701722716959267 1105 14-15 0.17280184303072588 1105 16-17 0.22018437387456657 1105 18-19 0.23358514947724274 1105 20-21 0.2052181562849995 1105 22-23 0.2674874336488031 1105 24-25 0.26439616203929006 1105 26-27 0.2142883470937207 1105 28-29 0.31661302179790596 1105 30-31 0.3146272172471356 1105 32-33 0.21983610462353198 1105 34-35 0.29307613019151546 1105 36-37 0.3259584882542512 1105 38-39 0.27370182350134087 1105 40-41 0.22134624338254838 1105 42-43 0.25052429493069184 1105 44-45 0.33185953318363914 1105 46-47 0.3405196551604561 1105 48-49 0.307931334109405 1105 50-51 0.30710826432299143 1105 52-53 0.25589729701344055 1105 54-55 0.23929608183880902 1105 56-57 0.27619474501086927 1105 58-59 0.21860781130824591 1105 60-61 0.2727337693552059 1105 62-63 0.3199626481765421 1105 64-65 0.3492743956858746 1105 66-67 0.38591710721537 1105 68-69 0.20926880372552858 1105 70-71 0.212077973614214 1105 72-73 0.2498893146058947 1105 74-75 0.3035653109990193 1105 76-77 0.1869219010627674 1105 78-79 0.24358887155291598 1105 80-81 0.14551497592098883 1105 82-83 0.18278615370466866 1105 84-85 0.1600881364893567 1105 86-87 0.2188239193179129 1105 88-89 0.3744136115011969 1105 90-91 0.29896269678166476 1105 92-93 0.3195676068943527 1105 94-95 0.3020593049045175 1105 96-97 0.3009701982398276 1105 98-99 0.29359064812091873 1105 100 0.3341781279287588 1106 1 0.01979091216337281 1106 2 -0.02201809211084438 1106 3 0.00712308187801014 1106 4 -0.014933747682228216 1106 5 -0.027470809693198817 1106 6 -0.0012223430188100792 1106 7 -0.016326969099900168 1106 8 -0.0020011862921620605 1106 9 -0.012881923844048515 1106 10-11 -0.006015863448830316 1106 12-13 0.014897294660251248 1106 14-15 0.04174081426585019 1106 16-17 -2.933550007639951E-4 1106 18-19 -0.008186923261597201 1106 20-21 -0.1083600563056919 1106 22-23 -0.06357083258954077 1106 24-25 -0.03156092590558757 1106 26-27 -2.6030160579182393E-4 1106 28-29 0.05946475669493623 1106 30-31 0.03630907376211212 1106 32-33 0.04340570784579256 1106 34-35 0.020933265676134738 1106 36-37 0.07493316590382904 1106 38-39 0.07539916837940552 1106 40-41 0.010967886533336468 1106 42-43 0.004298391903269305 1106 44-45 -0.06243050660280858 1106 46-47 -0.046893302485457866 1106 48-49 -0.03783170911410849 1106 50-51 0.020401754535441796 1106 52-53 -0.0913223869899582 1106 54-55 -0.04412696783263215 1106 56-57 -0.03416855455083123 1106 58-59 -0.019527865780943898 1106 60-61 -0.1309911789309055 1106 62-63 0.02500948059074659 1106 64-65 -0.009727008649043967 1106 66-67 -0.0017278723024674036 1106 68-69 -0.04721038352095519 1106 70-71 0.01855227331373044 1106 72-73 0.022808631223618647 1106 74-75 0.03247518159741247 1106 76-77 0.013378071302405203 1106 78-79 0.035007310537203296 1106 80-81 -7.030948883368637E-4 1106 82-83 -0.07247312003622142 1106 84-85 -0.05516893330177908 1106 86-87 -0.10718156444906413 1106 88-89 0.025218812094013998 1106 90-91 0.05586081662431752 1106 92-93 0.08963742292223031 1106 94-95 0.09395068236096549 1106 96-97 0.022569755749863418 1106 98-99 0.030196856944854744 1106 100 0.08146645009156828 1107 1 0.003266159031326765 1107 2 0.032848073238561426 1107 3 -0.0026174224717969707 1107 4 -0.031133450113948413 1107 5 -0.0324426402119542 1107 6 -0.03298349235259934 1107 7 -0.060024728808777184 1107 8 -0.005307368889155839 1107 9 -0.009224748089231127 1107 10-11 -0.010916855658990698 1107 12-13 0.028259141005086974 1107 14-15 -0.03793090111103936 1107 16-17 -0.06972751328055438 1107 18-19 8.060625831518564E-5 1107 20-21 -0.0058780501837460974 1107 22-23 0.018578091331534097 1107 24-25 -0.07663878881569275 1107 26-27 -0.029591625879405115 1107 28-29 0.0018544360982843955 1107 30-31 -0.042411595861281626 1107 32-33 -0.017469453258051715 1107 34-35 -0.013015816031945349 1107 36-37 -0.023834448906349337 1107 38-39 0.018590220956113512 1107 40-41 0.02774122526838596 1107 42-43 -0.042757198400174445 1107 44-45 -0.012008793595093437 1107 46-47 -0.056514992749100657 1107 48-49 -0.06359742621575748 1107 50-51 0.020824533671557788 1107 52-53 0.015675738993252253 1107 54-55 -0.01695710041160936 1107 56-57 -0.05490635093452312 1107 58-59 -0.061667301159346266 1107 60-61 -0.041524408968243165 1107 62-63 0.021510328755120156 1107 64-65 0.03638837487287816 1107 66-67 0.06368194745076039 1107 68-69 0.09055768853985313 1107 70-71 0.06767301353851707 1107 72-73 0.15145869945583001 1107 74-75 0.284729095473196 1107 76-77 0.1937232244458258 1107 78-79 0.12848467781606843 1107 80-81 0.10206712127512674 1107 82-83 0.11212641844864635 1107 84-85 0.13129698138899926 1107 86-87 0.10926778971405327 1107 88-89 0.17376923285934964 1107 90-91 0.12337339693932137 1107 92-93 0.2212865695456827 1107 94-95 0.13605558702539255 1107 96-97 0.1889749248141328 1107 98-99 0.21903461115724454 1107 100 0.4000362743936101 1108 1 -0.009838685240239897 1108 2 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-0.06974478152012153 2214 24-25 -0.17923770499325542 2214 26-27 -0.14506616435381403 2214 28-29 -0.097659768513644 2214 30-31 -0.16301507757394518 2214 32-33 -0.11265913343437006 2214 34-35 -0.20193386993014428 2214 36-37 -0.29808358115545275 2214 38-39 -0.2555222917102995 2214 40-41 -0.11526080923286486 2214 42-43 -0.14248083200186556 2214 44-45 -0.0647519393304492 2214 46-47 -0.10006182384972817 2214 48-49 -0.17797878304788384 2214 50-51 -0.05244040325283095 2214 52-53 -0.05152576887297755 2214 54-55 -0.16244039176353198 2214 56-57 -0.12386293391733716 2214 58-59 -0.07175920806049163 2214 60-61 -0.0017909459559888319 2214 62-63 -0.06515558872553129 2214 64-65 -0.1487812178939052 2214 66-67 -0.11819038315221064 2214 68-69 -0.0555454530018622 2214 70-71 0.06311363728424624 2214 72-73 0.13047389780789587 2214 74-75 0.126544823160053 2214 76-77 -0.013250184400057208 2214 78-79 -0.085370274595995 2214 80-81 0.018427898439753676 2214 82-83 -0.0981239199224575 2214 84-85 -0.018846843203384367 2214 86-87 -0.053742629821115884 2214 88-89 -0.09375885149224672 2214 90-91 -0.16574508109265196 2214 92-93 -0.16171810623000482 2214 94-95 -0.17849097882288234 2214 96-97 -0.2050874441345556 2214 98-99 -0.24813017422008343 2214 100 -0.23914257723246024 2215 1 0.033805226945858635 2215 2 0.061944732386685075 2215 3 0.031851230818279674 2215 4 0.016004760116615557 2215 5 0.05424246870892091 2215 6 0.04081447621562262 2215 7 0.12283453102456576 2215 8 0.07678827783912112 2215 9 0.0586156775279747 2215 10-11 0.035300299712943684 2215 12-13 -0.05202827510169783 2215 14-15 -0.015329813906639345 2215 16-17 0.031027831877558754 2215 18-19 0.05374077905116792 2215 20-21 0.06470017940439021 2215 22-23 0.034781680589283326 2215 24-25 0.15866606291579188 2215 26-27 0.031140202676446904 2215 28-29 0.10523269695428894 2215 30-31 0.11984232553966478 2215 32-33 0.050183426370438156 2215 34-35 0.08948367619919395 2215 36-37 -0.05845128496292773 2215 38-39 0.10152193250242192 2215 40-41 0.1295082664814018 2215 42-43 0.10476791834211951 2215 44-45 0.16116755898534763 2215 46-47 0.2231021851606414 2215 48-49 0.23547999339195513 2215 50-51 0.19394326116714922 2215 52-53 0.0036687034993221346 2215 54-55 0.1105503145727269 2215 56-57 0.13565587948029645 2215 58-59 0.17560958119328518 2215 60-61 0.17474442747494834 2215 62-63 0.3636339094520551 2215 64-65 0.1610297631129427 2215 66-67 0.20226977542935742 2215 68-69 0.3037792436844384 2215 70-71 0.25406424566193664 2215 72-73 0.32330889561496434 2215 74-75 0.34282358814083835 2215 76-77 0.2603830596169274 2215 78-79 0.2515985558963081 2215 80-81 0.26715700415867616 2215 82-83 0.27217856052060085 2215 84-85 0.31537424621629384 2215 86-87 0.23436887023728303 2215 88-89 0.3173350330228999 2215 90-91 0.32004747747680895 2215 92-93 0.32360870457122104 2215 94-95 0.3014054787459095 2215 96-97 0.1859003899125966 2215 98-99 0.16080994704189422 2215 100 0.18073992177752984 2216 1 0.06608245525614365 2216 2 0.05896094006176611 2216 3 0.038596578729059416 2216 4 -0.05138243957431854 2216 5 0.0700524143089396 2216 6 0.047567506392937275 2216 7 0.05074663190830364 2216 8 0.050953822100154866 2216 9 -0.05922870179204409 2216 10-11 -0.03861857491659748 2216 12-13 1.7495125951683121E-4 2216 14-15 -0.08777218339738369 2216 16-17 -0.13084589518412315 2216 18-19 -0.18163676832218556 2216 20-21 -0.08666476332987116 2216 22-23 -0.07139964061009607 2216 24-25 -0.08376902378626738 2216 26-27 -0.1816121690511565 2216 28-29 -0.09735150625002831 2216 30-31 -0.16572323073749828 2216 32-33 -0.13626465279272537 2216 34-35 -0.20425642096187602 2216 36-37 -0.114395854601824 2216 38-39 -0.10933803207014137 2216 40-41 -0.05839705917305338 2216 42-43 -0.05965224166306626 2216 44-45 -0.12910004773643635 2216 46-47 -0.027281716241873255 2216 48-49 0.020493141723598285 2216 50-51 0.061900490333613334 2216 52-53 -0.0050669607290458885 2216 54-55 -0.011890497272240452 2216 56-57 -0.03514034983016501 2216 58-59 -0.07163163741975609 2216 60-61 -0.007704502141606895 2216 62-63 0.09058520250467694 2216 64-65 0.10404326620635374 2216 66-67 0.018448236706333887 2216 68-69 -0.0015900566433302288 2216 70-71 -0.02943210536741958 2216 72-73 0.10865182510585214 2216 74-75 0.027066618391266672 2216 76-77 -0.026979670200724115 2216 78-79 0.06225060654755765 2216 80-81 0.05120290215153034 2216 82-83 0.010075652904561139 2216 84-85 0.044515382591995944 2216 86-87 -0.023443438533881533 2216 88-89 0.04985181029831409 2216 90-91 0.0016010256826106684 2216 92-93 0.03919593861512993 2216 94-95 0.10085808281704445 2216 96-97 -0.007123206089616474 2216 98-99 -0.09645914601303218 2216 100 -0.09991748738708495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36278.0 3 6113.0 4 4038.0 5 3199.0 6 2699.0 7 3014.0 8 3474.0 9 3787.0 10 4225.0 11 4604.0 12 5023.0 13 5569.0 14 5881.0 15 6170.0 16 6376.0 17 6304.0 18 6227.0 19 6181.0 20 6471.0 21 6791.0 22 7651.0 23 10279.0 24 15367.0 25 20483.0 26 21368.0 27 19213.0 28 20521.0 29 25375.0 30 31081.0 31 38000.0 32 49591.0 33 66719.0 34 92647.0 35 142690.0 36 261752.0 37 534284.0 38 813706.0 39 327205.0 40 644.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31144732868208 19.59562625649411 13.202073234349307 23.8908531804745 2 15.496352006539105 19.830182698392584 40.52923969598342 24.14422559908489 3 18.4663809375662 25.151704048648742 29.501231698054653 26.8806833157304 4 12.217465548334994 16.42835768514621 38.91936430152369 32.4348124649951 5 13.076860190767349 37.97722731429462 35.576722353088805 13.36919014184923 6 31.47918314657268 37.87653536339167 17.638570905631102 13.00571058440455 7 27.720655704803598 32.19797036511077 22.329062671762166 17.752311258323456 8 25.102696217631586 36.72415221924949 20.24759615201853 17.92555541110039 9 24.917439995498974 17.065847662298314 20.85971128803491 37.15700105416781 10-11 23.951936229243824 26.985879071875157 28.74662316540844 20.315561533472575 12-13 24.59690686162609 24.174278942789655 28.697623646939658 22.5311905486446 14-15 21.855026933138642 26.190426860642795 25.66366298668825 26.290883219530315 16-17 21.828996361669205 29.530259769709875 26.256161221715786 22.384582646905137 18-19 21.8383717040236 28.603144673215535 27.95276907636629 21.605714546394577 20-21 22.86077082677925 27.89913538368834 27.45290326505047 21.78719052448194 22-23 22.403942414772764 27.634841947782814 27.59171442088323 22.3695012165612 24-25 22.31227436274107 27.81290014021065 27.561657709608838 22.313167787439436 26-27 22.049057336836377 27.76704037638858 27.595246178158835 22.588656108616206 28-29 22.136519622471802 27.822480240290837 27.692560936620286 22.348439200617072 30-31 22.384226580199858 28.287473951705234 27.220622475123555 22.107676992971356 32-33 21.98095645791866 27.901596770456145 27.800374134071237 22.31707263755396 34-35 22.080390059355867 27.661434348549026 27.91417386471546 22.344001727379652 36-37 22.453123313207122 27.954785968316042 27.29143447527152 22.300656243205314 38-39 22.28664692364026 28.089336655980397 27.516965020022745 22.107051400356607 40-41 22.05482917413369 27.839316358226192 27.756316724473717 22.349537743166405 42-43 22.011901098748634 27.741298112659603 27.765625632539276 22.481175156052487 44-45 22.18153482117827 27.81450244938492 27.701569411910043 22.30239331752676 46-47 22.174356954861782 27.98557757366301 27.574727341563136 22.26533812991207 48-49 22.1392038241255 27.853213150296707 27.64216177425314 22.365421251324655 50-51 22.157928986615623 27.80797528382699 27.598459496401894 22.435636233155492 52-53 22.225681247408108 27.737865948709878 27.630123015859443 22.406329788022575 54-55 22.258341986872974 28.044082119177105 27.523558406451308 22.174017487498613 56-57 22.036028447832525 27.864082577926137 27.537694974497523 22.562193999743815 58-59 22.000071457865896 27.554020056094426 27.869346894110464 22.576561591929213 60-61 22.238853624235052 27.896860389980617 27.550267969136037 22.314018016648298 62-63 22.100028051002653 27.946020444543773 27.48880430510673 22.465147199346845 64-65 22.007398121886567 27.676383860052955 27.683510595840723 22.632707422219756 66-67 21.98097227598923 27.70283971515238 27.528891174501386 22.78729683435701 68-69 22.32264334859225 27.837899320789056 27.53844139342105 22.301015937197647 70-71 22.25591110695543 27.633196842037645 27.611341510648106 22.499550540358815 72-73 22.124908279206743 27.783689254038425 27.636555893477883 22.454846573276956 74-75 22.124848913316153 27.787710856195886 27.57778588641322 22.509654344074743 76-77 22.156205736535497 27.786751230611024 27.445747353053136 22.611295679800342 78-79 22.278073059331224 27.843738104192965 27.54131497521871 22.336873861257097 80-81 22.220532900377037 27.64706966748508 27.632055819586004 22.500341612551882 82-83 22.093982991208254 27.689639437864926 27.813663857334287 22.402713713592533 84-85 22.042194673393325 27.633613203267522 27.612593975995892 22.711598147343263 86-87 22.178015318879797 27.755915051541535 27.585139360071974 22.480930269506693 88-89 22.03626055043907 27.57278913957666 27.663639346092637 22.727310963891632 90-91 21.983702193620726 27.619387961328705 27.70122307161203 22.695686773438542 92-93 22.2112050439217 27.726751629378217 27.543049847272083 22.518993479428 94-95 21.9940022561794 27.488970968465292 27.70367314494624 22.81335363040906 96-97 22.030303065060707 27.49034509891184 27.730221742519912 22.749130093507542 98-99 21.94329891093551 27.58695110689027 27.635582516931628 22.83416746524259 100 22.152030855484202 27.50383476044026 27.473554299137316 22.870580084938226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 400.0 1 373.5 2 301.5 3 227.5 4 176.5 5 151.0 6 144.0 7 140.5 8 140.0 9 147.5 10 152.0 11 158.5 12 181.5 13 209.5 14 236.5 15 285.5 16 382.5 17 533.0 18 769.5 19 1053.5 20 1572.0 21 2447.5 22 3682.0 23 5486.0 24 8029.5 25 11301.0 26 15496.0 27 20899.5 28 27139.5 29 33852.5 30 40231.5 31 46961.0 32 54205.0 33 61564.0 34 68809.5 35 74327.0 36 78903.0 37 83297.5 38 86598.0 39 88487.5 40 90560.5 41 92128.0 42 93114.5 43 94194.0 44 93102.0 45 91945.0 46 89950.5 47 86536.0 48 85418.5 49 85144.5 50 82745.5 51 79294.0 52 75527.5 53 72362.0 54 70302.0 55 67785.5 56 63133.5 57 58305.0 58 52956.5 59 46113.0 60 40176.5 61 35144.0 62 30681.5 63 25772.0 64 21071.5 65 17546.0 66 14630.5 67 12001.0 68 9881.0 69 8044.5 70 6396.0 71 4810.0 72 3650.0 73 2910.0 74 2322.0 75 1751.0 76 1177.5 77 902.0 78 727.0 79 525.0 80 393.5 81 218.5 82 111.5 83 79.0 84 55.5 85 38.0 86 26.5 87 14.0 88 10.5 89 13.0 90 9.5 91 7.5 92 8.5 93 7.0 94 3.5 95 2.0 96 3.5 97 2.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32778411250475104 2 0.11847206385404789 3 0.06472824021284682 4 0.03842645381984036 5 0.04161915621436716 6 0.010300266058532877 7 0.006917521854808058 8 0.01619156214367161 9 0.018586088939566703 10-11 0.021968833143291525 12-13 0.016552641581147852 14-15 0.01917521854808058 16-17 0.020353477765108322 18-19 0.01976434815659445 20-21 0.024420372481946027 22-23 0.016324591410110226 24-25 0.025541619156214366 26-27 0.020030406689471685 28-29 0.019802356518434053 30-31 0.02088559483086279 32-33 0.02506651463321931 34-35 0.01619156214367161 36-37 0.010186240973014063 38-39 0.013930064614215129 40-41 0.010091220068415051 42-43 0.008912960851387305 44-45 0.00902698593690612 46-47 0.008969973394146713 48-49 0.0047130368681109844 50-51 0.00653743823641201 52-53 0.007639680729760548 54-55 0.0034967692892436337 56-57 0.0030026605853287723 58-59 0.0030026605853287723 60-61 0.003800836183960471 62-63 0.0029646522234891676 64-65 0.0023565184340554922 66-67 0.0018814139110604332 68-69 0.0030216647662485744 70-71 0.002394526795895097 72-73 0.002033447358418852 74-75 0.002280501710376283 76-77 0.003477765108323831 78-79 0.0031736982136069936 80-81 0.0036297985556822503 82-83 0.002527556062333713 84-85 0.0028696313188901557 86-87 0.0024135309768148995 88-89 0.5969593310528316 90-91 0.6075826681870011 92-93 0.6040288863549981 94-95 0.5897567464842266 96-97 0.5887115165336374 98-99 0.58557582668187 100 0.587305207145572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2631000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.71422820297406 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86745210147964 54.08162877589693 2 12.636498772121673 17.11341523084097 3 3.6543923461406758 7.423630718093145 4 1.4589119695023895 3.9515639212374056 5 0.7543665811459386 2.5540675412206717 6 0.4008600144131996 1.628635589605372 7 0.26669072706654035 1.2641129726540548 8 0.17818374227122186 0.9652459668970745 9 0.12717683700321655 0.7750513226671416 >10 0.606941340830705 7.14193654813548 >50 0.03605137806470255 1.6582622629680182 >100 0.01213725672266051 1.3032765876227763 >500 3.369332373784729E-4 0.13917256216096197 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.800836183960471E-5 0.0 9 0.0 0.0 0.0 3.800836183960471E-5 0.0 10-11 0.0 0.0 0.0 7.601672367920942E-5 0.0 12-13 0.0 0.0 0.0 7.601672367920942E-5 0.0 14-15 0.0 0.0 0.0 7.601672367920942E-5 0.0 16-17 0.0 3.800836183960471E-5 0.0 7.601672367920942E-5 0.0 18-19 0.0 3.800836183960471E-5 0.0 7.601672367920942E-5 0.0 20-21 0.0 3.800836183960471E-5 0.0 1.1402508551881414E-4 0.0 22-23 0.0 7.601672367920942E-5 0.0 2.470543519574306E-4 0.0 24-25 0.0 7.601672367920942E-5 0.0 3.4207525655644244E-4 0.0 26-27 0.0 7.601672367920942E-5 0.0 4.5610034207525655E-4 0.0 28-29 0.0 1.5203344735841885E-4 0.0 0.0011402508551881414 0.0 30-31 0.0 1.5203344735841885E-4 0.0 0.004142911440516914 0.0 32-33 0.0 1.5203344735841885E-4 0.0 0.011668567084758646 0.0 34-35 0.0 1.5203344735841885E-4 0.0 0.02210186240973014 0.0 36-37 0.0 1.5203344735841885E-4 0.0 0.04180919802356518 0.0 38-39 0.0 1.9004180919802356E-4 0.0 0.07537058152793615 0.0 40-41 0.0 1.9004180919802356E-4 0.0 0.11440516913721019 0.0 42-43 0.0 1.9004180919802356E-4 0.0 0.15589129608513874 0.0 44-45 0.0 1.9004180919802356E-4 0.0 0.2022805017103763 0.0 46-47 0.0 1.9004180919802356E-4 0.0 0.25923603192702394 0.0 48-49 0.0 2.2805017103762827E-4 0.0 0.32120866590649944 0.0 50-51 0.0 2.2805017103762827E-4 0.0 0.39220828582288103 0.0 52-53 0.0 2.4705435195743067E-4 0.0 0.46356898517673883 0.0 54-55 0.0 2.66058532877233E-4 0.0 0.5365450399087799 0.0 56-57 0.0 3.800836183960472E-4 0.0 0.6259597111364501 0.0 58-59 0.0 4.1809198023565186E-4 0.0 0.7316419612314708 0.0 60-61 0.0 4.1809198023565186E-4 0.0 0.8405169137210187 0.0 62-63 0.0 4.1809198023565186E-4 0.0 0.9536868110984417 0.0 64-65 0.0 4.1809198023565186E-4 0.0 1.0728430254656025 0.0 66-67 0.0 4.1809198023565186E-4 0.0 1.209236031927024 0.0 68-69 0.0 5.32117065754466E-4 0.0 1.3657164576206766 0.0 70-71 0.0 5.32117065754466E-4 0.0 1.532288103382744 0.0 72-73 0.0 5.32117065754466E-4 0.0 1.7110034207525655 0.0 74-75 0.0 5.32117065754466E-4 0.0 1.8945077917141773 0.0 76-77 0.0 5.32117065754466E-4 0.0 2.086830102622577 0.0 78-79 0.0 5.32117065754466E-4 0.0 2.306936526035728 0.0 80-81 0.0 5.32117065754466E-4 0.0 2.5563854047890535 0.0 82-83 0.0 5.32117065754466E-4 0.0 2.8160965412390726 0.0 84-85 0.0 5.701254275940707E-4 0.0 3.068187001140251 0.0 86-87 0.0 6.841505131128848E-4 0.0 3.3415621436716076 0.0 88 0.0 7.221588749524896E-4 0.0 3.5648422652983656 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 2855 0.0 36.03495 4 ATCAACG 2885 0.0 35.504833 3 CAACGCA 2950 0.0 34.71592 5 GTATCAA 3160 0.0 33.98272 1 AACGCAG 3130 0.0 32.710144 6 GGTATCA 2080 0.0 29.436785 1 GTACATG 7205 0.0 28.239702 1 TATCAAC 3745 0.0 27.86663 2 TACATGG 7330 0.0 26.615055 2 ACGCAGA 3800 0.0 26.44847 7 ACATGGG 7375 0.0 25.420708 3 CGCAGAG 3900 0.0 25.288614 8 GTATCGT 145 2.549691E-5 22.67255 6 CATGGGG 4985 0.0 21.20329 4 GAGTACA 5125 0.0 20.309946 1 GCAGAGT 5020 0.0 18.61743 9 AGTACAT 5235 0.0 18.049395 2 GTACGAC 185 1.6542262E-4 17.778828 3 CTGTCGC 700 0.0 17.443983 9 TCTGTCG 700 0.0 16.77306 8 >>END_MODULE