##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033104_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2631000
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74554199923983	34.0	31.0	34.0	31.0	34.0
2	32.8533325731661	34.0	31.0	34.0	31.0	34.0
3	32.943049410870394	34.0	31.0	34.0	31.0	34.0
4	36.364616495629036	37.0	37.0	37.0	35.0	37.0
5	36.31013264918282	37.0	37.0	37.0	35.0	37.0
6	36.39435727860129	37.0	37.0	37.0	35.0	37.0
7	36.36382668187001	37.0	37.0	37.0	35.0	37.0
8	36.36345496009122	37.0	37.0	37.0	35.0	37.0
9	38.18312732801216	39.0	39.0	39.0	37.0	39.0
10-11	38.164958380843785	39.0	39.0	39.0	37.0	39.0
12-13	38.12990440896998	39.0	39.0	39.0	37.0	39.0
14-15	39.66489566704675	41.0	40.0	41.0	37.0	41.0
16-17	39.67442949448879	41.0	40.0	41.0	37.0	41.0
18-19	39.68617008741923	41.0	40.0	41.0	37.0	41.0
20-21	39.676288483466365	41.0	40.0	41.0	37.0	41.0
22-23	39.61014006081338	41.0	40.0	41.0	37.0	41.0
24-25	39.56510452299506	41.0	40.0	41.0	37.0	41.0
26-27	39.46438920562524	41.0	39.0	41.0	36.5	41.0
28-29	39.37614291144052	41.0	39.0	41.0	36.0	41.0
30-31	39.28586735081718	41.0	39.0	41.0	36.0	41.0
32-33	39.32734549600912	41.0	39.0	41.0	36.0	41.0
34-35	39.27146997339415	41.0	39.0	41.0	35.0	41.0
36-37	39.18958760927404	41.0	39.0	41.0	35.0	41.0
38-39	39.09968053971873	41.0	39.0	41.0	35.0	41.0
40-41	38.988194222729	41.0	39.0	41.0	35.0	41.0
42-43	38.875392816419605	41.0	39.0	41.0	35.0	41.0
44-45	38.73803781832003	41.0	39.0	41.0	35.0	41.0
46-47	38.5326383504371	41.0	39.0	41.0	35.0	41.0
48-49	38.347428544279744	41.0	38.0	41.0	35.0	41.0
50-51	38.21305454199924	40.5	38.0	41.0	34.0	41.0
52-53	38.07760984416572	40.0	38.0	41.0	34.0	41.0
54-55	37.93790516913721	40.0	37.5	41.0	34.0	41.0
56-57	37.77811231470923	40.0	37.0	41.0	34.0	41.0
58-59	37.5867459141011	40.0	37.0	41.0	33.5	41.0
60-61	37.39308456860509	40.0	36.0	41.0	33.0	41.0
62-63	37.15864652223489	39.0	36.0	41.0	33.0	41.0
64-65	36.904106423413154	39.0	35.0	41.0	33.0	41.0
66-67	36.62336012922843	39.0	35.0	41.0	32.5	41.0
68-69	36.30078848346636	38.0	35.0	40.5	32.0	41.0
70-71	35.96691619156215	37.0	35.0	40.0	32.0	41.0
72-73	35.61256271379703	37.0	35.0	39.0	31.0	41.0
74-75	35.2778821740783	36.0	35.0	39.0	31.0	41.0
76-77	34.94554599011783	36.0	35.0	39.0	31.0	40.5
78-79	34.6080267958951	35.0	35.0	37.5	31.0	39.5
80-81	34.273882554161915	35.0	35.0	37.0	31.0	39.0
82-83	33.9579688331433	35.0	34.5	36.5	30.5	39.0
84-85	33.69260243253515	35.0	34.0	36.0	30.0	37.5
86-87	33.46946180159635	35.0	34.0	36.0	30.0	37.0
88-89	33.2602727099962	35.0	34.0	35.5	30.0	37.0
90-91	33.06608171797795	35.0	34.0	35.0	30.0	36.0
92-93	32.905517673888255	35.0	34.0	35.0	30.0	36.0
94-95	32.761570505511216	35.0	34.0	35.0	29.5	36.0
96-97	32.64368320030407	35.0	34.0	35.0	29.5	36.0
98-99	32.542123907259594	35.0	34.0	35.0	29.5	35.5
100	32.45117179779552	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1270223147743934
1101	2	0.15123497655041973
1101	3	0.1715125073840298
1101	4	0.09501201589161923
1101	5	0.10651465315191899
1101	6	0.12202227844321101
1101	7	0.16484134896212055
1101	8	0.16926594886788138
1101	9	0.20719854178411623
1101	10-11	0.17375305930342932
1101	12-13	0.1911784143544608
1101	14-15	0.18108297476432966
1101	16-17	0.21271116851027472
1101	18-19	0.2577447234504149
1101	20-21	0.2632528512181125
1101	22-23	0.27226361717375624
1101	24-25	0.1881468793587473
1101	26-27	0.25630161696179243
1101	28-29	0.29962036399531655
1101	30-31	0.25383842682106916
1101	32-33	0.25577417487217247
1101	34-35	0.2668446946307199
1101	36-37	0.30539330159629685
1101	38-39	0.24450620803851564
1101	40-41	0.3274605947224245
1101	42-43	0.24210251704781882
1101	44-45	0.25010601122298937
1101	46-47	0.27761567223670625
1101	48-49	0.18010143505951248
1101	50-51	0.19615820589169886
1101	52-53	0.22750071171272168
1101	54-55	0.20443683756381859
1101	56-57	0.20232142657185648
1101	58-59	0.18923520434387342
1101	60-61	0.2083749022083552
1101	62-63	0.24024319982203934
1101	64-65	0.2146438915762019
1101	66-67	0.24998505383094027
1101	68-69	0.23524785497868805
1101	70-71	0.1959504395494207
1101	72-73	0.2423454965458518
1101	74-75	0.22872788335272531
1101	76-77	0.19578467536221922
1101	78-79	0.1808698206283168
1101	80-81	0.17756409981037535
1101	82-83	0.1927518235121468
1101	84-85	0.23950759784308673
1101	86-87	0.16070681265793496
1101	88-89	0.1796094260493888
1101	90-91	0.185579771349758
1101	92-93	0.19350434050024745
1101	94-95	0.17942709271589763
1101	96-97	0.1955297599284478
1101	98-99	0.2039398761794473
1101	100	0.2479925664229654
1102	1	0.0623970543200727
1102	2	0.11789587140766855
1102	3	0.06266820165956233
1102	4	0.04132752068397849
1102	5	0.026165691142111314
1102	6	0.02774368374839753
1102	7	0.0697118829678871
1102	8	0.10572220529950727
1102	9	0.11542493505525186
1102	10-11	0.08321871335206055
1102	12-13	0.09064507194952398
1102	14-15	0.14725379877800293
1102	16-17	0.10259824402289297
1102	18-19	0.10321415355510055
1102	20-21	0.1160296982112996
1102	22-23	0.14854229691487575
1102	24-25	0.14206008109709245
1102	26-27	0.13822447996069798
1102	28-29	0.13958529089801885
1102	30-31	0.1432209395992814
1102	32-33	0.12056187377422845
1102	34-35	0.1207879687434783
1102	36-37	0.1362348007926073
1102	38-39	0.10159313386962765
1102	40-41	0.11481631791369296
1102	42-43	0.18268314519983875
1102	44-45	0.1397513170920348
1102	46-47	0.21482805838095942
1102	48-49	0.08538417150951005
1102	50-51	0.0423166471629699
1102	52-53	0.07243550670860088
1102	54-55	0.03324355282027369
1102	56-57	0.14744988901965428
1102	58-59	0.0817319087994477
1102	60-61	0.05076055319531747
1102	62-63	0.08256622270800307
1102	64-65	0.05494764296884114
1102	66-67	0.06533118379870473
1102	68-69	0.04911509769234357
1102	70-71	0.09189927569070022
1102	72-73	0.13537105296131813
1102	74-75	0.0640185631278527
1102	76-77	0.04481591287104436
1102	78-79	0.04822755091245057
1102	80-81	0.11900770985604225
1102	82-83	0.12958850908744068
1102	84-85	0.07520190739464283
1102	86-87	0.10365887205427526
1102	88-89	0.14668789827752704
1102	90-91	0.120820920155289
1102	92-93	0.1838829836714595
1102	94-95	0.14420179654213428
1102	96-97	0.1245483085281549
1102	98-99	0.040496453235661534
1102	100	0.029186514527708596
1103	1	0.10485603859779502
1103	2	0.1205225378529704
1103	3	0.09660707237827637
1103	4	0.10758827475566335
1103	5	0.033699302285498334
1103	6	0.07647212164788897
1103	7	0.11814061804665954
1103	8	0.12882190147934125
1103	9	0.15856268346558977
1103	10-11	0.1362801580750599
1103	12-13	0.10247599519757244
1103	14-15	0.19832689824175276
1103	16-17	0.1574943640899633
1103	18-19	0.11949373451570722
1103	20-21	0.09200947673802062
1103	22-23	0.10700317384280567
1103	24-25	0.06385916133550751
1103	26-27	0.14229308022964915
1103	28-29	0.10800964299736648
1103	30-31	0.1446949820676906
1103	32-33	0.12905087482016597
1103	34-35	0.1537630488490649
1103	36-37	0.18316279707372018
1103	38-39	0.203182760818315
1103	40-41	0.2457611820247365
1103	42-43	0.19795480691352196
1103	44-45	0.19645007026720407
1103	46-47	0.2192617741181806
1103	48-49	0.19107191742951812
1103	50-51	0.1713987450163117
1103	52-53	0.14647933650289957
1103	54-55	0.11807528957587721
1103	56-57	0.17981935426296047
1103	58-59	0.16987395717823972
1103	60-61	0.15615407501890388
1103	62-63	0.037208727647147555
1103	64-65	0.13610355418030196
1103	66-67	0.1491881392882206
1103	68-69	0.12672949511568277
1103	70-71	0.1543990349588782
1103	72-73	0.14330982406686132
1103	74-75	0.09625697064533512
1103	76-77	0.03186802716055581
1103	78-79	0.06233154706074373
1103	80-81	0.0975873256812676
1103	82-83	0.04352326246183935
1103	84-85	0.07033507359921032
1103	86-87	0.10319845998780153
1103	88-89	0.10294712494728486
1103	90-91	0.09186320594546515
1103	92-93	0.14285411245467827
1103	94-95	0.16366282288981893
1103	96-97	0.1204527462948306
1103	98-99	0.1726552034224369
1103	100	0.23753529343627378
1104	1	0.07124555895635609
1104	2	0.08536818432395421
1104	3	0.08675857647998697
1104	4	0.02954235678166839
1104	5	0.012230794968672853
1104	6	0.03900658044423011
1104	7	0.04652323206021691
1104	8	0.08303949666688482
1104	9	0.08491709908815892
1104	10-11	0.10887089436290154
1104	12-13	0.06820700873938534
1104	14-15	0.07356717216183739
1104	16-17	0.18958818786512666
1104	18-19	0.09165979153342363
1104	20-21	0.06924375071227473
1104	22-23	0.09693621751041093
1104	24-25	0.05457996486358496
1104	26-27	0.005803949726924884
1104	28-29	0.09190796949276603
1104	30-31	0.040133111468158233
1104	32-33	0.09283358593303603
1104	34-35	0.06979812285082687
1104	36-37	0.05894111527609169
1104	38-39	0.02895675539242859
1104	40-41	0.03155776364963003
1104	42-43	0.03615473981914619
1104	44-45	0.12490300835493429
1104	46-47	0.14046215136777107
1104	48-49	0.07549501779395484
1104	50-51	0.0489692852719017
1104	52-53	0.12118051127957585
1104	54-55	0.07767495545838443
1104	56-57	0.06243323758960173
1104	58-59	0.030021382483660375
1104	60-61	0.13626525477557294
1104	62-63	0.11082670331398248
1104	64-65	0.14113263709187152
1104	66-67	0.11633748522224607
1104	68-69	0.10636885242657712
1104	70-71	0.17037054381138006
1104	72-73	0.14725122796458834
1104	74-75	0.060969261834927124
1104	76-77	0.039828972816195574
1104	78-79	0.11557494545339608
1104	80-81	0.15615920521976534
1104	82-83	0.1957203818835751
1104	84-85	0.1634135438057669
1104	86-87	0.13224588393882186
1104	88-89	0.1578628688926429
1104	90-91	0.23235742270197335
1104	92-93	0.1865228230341316
1104	94-95	0.16643834479863528
1104	96-97	0.23173264965392093
1104	98-99	0.22229866036639123
1104	100	0.28209731510234093
1105	1	0.05161377692635227
1105	2	0.06571397020850611
1105	3	0.06724504986963353
1105	4	0.07576263206922107
1105	5	0.06851377704612815
1105	6	0.04087152245006109
1105	7	-0.04486703193715158
1105	8	-0.05701243266840095
1105	9	-0.030222397065834627
1105	10-11	-0.042491612913387655
1105	12-13	-9.030297938750209E-5
1105	14-15	-5.677284935572402E-4
1105	16-17	0.013113652115045227
1105	18-19	0.0037016051920346627
1105	20-21	0.05377780173829905
1105	22-23	0.09953227584740176
1105	24-25	0.026835716525688724
1105	26-27	-0.006904800621178708
1105	28-29	0.07929111529303157
1105	30-31	0.05917858036283263
1105	32-33	0.033833996622419704
1105	34-35	0.11402580688440622
1105	36-37	0.04230188026424031
1105	38-39	0.04641215387171371
1105	40-41	0.09038948932922608
1105	42-43	0.0650915016967133
1105	44-45	0.1568600588913398
1105	46-47	0.22108969555583258
1105	48-49	0.1784331874262648
1105	50-51	0.23206270001589502
1105	52-53	0.19091118299046883
1105	54-55	0.19096826584605964
1105	56-57	0.16033421017444738
1105	58-59	0.16358937093006887
1105	60-61	0.16926750225690057
1105	62-63	0.16919957660147844
1105	64-65	0.08713998978909387
1105	66-67	0.10072761274204822
1105	68-69	0.09139000016516974
1105	70-71	0.188708490907338
1105	72-73	0.1918355947237842
1105	74-75	0.10463812303397191
1105	76-77	0.07239219233947125
1105	78-79	0.10189564610858781
1105	80-81	0.1227117719530284
1105	82-83	0.17885073102485194
1105	84-85	0.16577909204342234
1105	86-87	0.09536367111224564
1105	88-89	0.10152795370980527
1105	90-91	0.06121166969268188
1105	92-93	0.06978709863737009
1105	94-95	0.07766418349472559
1105	96-97	0.10485997894637933
1105	98-99	0.06685203691677799
1105	100	0.08430965910823573
1106	1	0.0012736218727198434
1106	2	0.034534092087533
1106	3	0.033030217591417
1106	4	0.04324344465274521
1106	5	-0.00948096868911108
1106	6	-0.0032382548902987196
1106	7	0.027429684056301085
1106	8	0.04867764342554182
1106	9	0.059971665869980484
1106	10-11	0.004541372867798543
1106	12-13	0.029434093165704667
1106	14-15	-0.004700771771716461
1106	16-17	0.020316833817041413
1106	18-19	-0.010668329290545842
1106	20-21	0.006168510420465623
1106	22-23	0.03617239392568905
1106	24-25	0.022698301776962637
1106	26-27	-0.046432669656780945
1106	28-29	-0.08730152982916906
1106	30-31	-0.033250548688442905
1106	32-33	-0.012434892532262154
1106	34-35	0.015116701293358403
1106	36-37	-0.042029888851679686
1106	38-39	-0.10756184680707293
1106	40-41	-0.03529205025414228
1106	42-43	0.016597807239428164
1106	44-45	0.06652929642794447
1106	46-47	0.051954645651250075
1106	48-49	-0.017370777618971545
1106	50-51	-0.03932811977448125
1106	52-53	-0.0504278105877205
1106	54-55	-0.03825972030852398
1106	56-57	-0.011988552497811611
1106	58-59	-0.0298084334988431
1106	60-61	0.032895106393525
1106	62-63	-0.03783238916991394
1106	64-65	-0.04449773915908395
1106	66-67	-0.0025583973985447983
1106	68-69	-0.0018728351099284168
1106	70-71	0.030595071889138126
1106	72-73	0.0700179494042601
1106	74-75	0.0034465937296417337
1106	76-77	0.001443407152926568
1106	78-79	0.025472444042826226
1106	80-81	0.04996977311024864
1106	82-83	0.04338817783103366
1106	84-85	0.057493728516178066
1106	86-87	0.060401044149863026
1106	88-89	0.08800866489949755
1106	90-91	0.01613364197877587
1106	92-93	-0.02597946604854684
1106	94-95	0.0052040837887687985
1106	96-97	0.04839135608710876
1106	98-99	0.039714372629930494
1106	100	-0.028930371662056587
1107	1	0.010047029313824396
1107	2	0.02094000744167346
1107	3	-0.005309417745287703
1107	4	0.04599168672548615
1107	5	-0.11231805479437895
1107	6	-0.12335846678923446
1107	7	-0.08230762190833474
1107	8	-0.02542977019436421
1107	9	0.001705380166526993
1107	10-11	0.011761786620375858
1107	12-13	-0.013782191116241904
1107	14-15	0.03250367374675278
1107	16-17	-0.009440171831073485
1107	18-19	-0.0051830394931613455
1107	20-21	-0.0038538664298641834
1107	22-23	0.027979374934382406
1107	24-25	-0.01962640302394192
1107	26-27	0.013864827547592995
1107	28-29	-0.013556244485826596
1107	30-31	0.043595689536758186
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2216	9	-0.13715871431490712
2216	10-11	-0.16839270264163275
2216	12-13	-0.11239340274521936
2216	14-15	-0.2509892149491506
2216	16-17	-0.20389852124726815
2216	18-19	-0.15837444829799097
2216	20-21	-0.17147428013460342
2216	22-23	-0.2177437573096057
2216	24-25	-0.2312791714200486
2216	26-27	-0.25770999259243155
2216	28-29	-0.26360583330073695
2216	30-31	-0.21843244608462697
2216	32-33	-0.2484904024345127
2216	34-35	-0.23328475690681927
2216	36-37	-0.28679852807902506
2216	38-39	-0.299641506740727
2216	40-41	-0.3226735912164216
2216	42-43	-0.32170621216716455
2216	44-45	-0.1886170703021861
2216	46-47	-0.26710284481509916
2216	48-49	-0.2971487335009755
2216	50-51	-0.23788508100073358
2216	52-53	-0.23307831711388616
2216	54-55	-0.21835358574514174
2216	56-57	-0.1392820671367332
2216	58-59	-0.14771484085476771
2216	60-61	-0.19289128576718895
2216	62-63	-0.24641909831105835
2216	64-65	-0.16066960297473543
2216	66-67	-0.14392017330060014
2216	68-69	-0.06943092470537238
2216	70-71	-0.05755812254543713
2216	72-73	-0.016666336053980046
2216	74-75	-0.0123169266920371
2216	76-77	-0.03246245236341849
2216	78-79	-0.07203809848744669
2216	80-81	-0.03911796125743905
2216	82-83	-0.07902545007316064
2216	84-85	-0.0864141487632466
2216	86-87	-0.14627577896671085
2216	88-89	-0.1262318952946586
2216	90-91	-0.13008525037074747
2216	92-93	-0.08882089354266043
2216	94-95	-0.17025754996807052
2216	96-97	-0.2389164068899845
2216	98-99	-0.21508001280339784
2216	100	-0.17285729933032457
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17.0
3	9.0
4	13.0
5	16.0
6	48.0
7	135.0
8	279.0
9	519.0
10	978.0
11	1527.0
12	2460.0
13	3696.0
14	4666.0
15	6021.0
16	7540.0
17	10889.0
18	13308.0
19	7027.0
20	4733.0
21	4218.0
22	4503.0
23	4930.0
24	5655.0
25	6855.0
26	8947.0
27	11519.0
28	14652.0
29	19201.0
30	24496.0
31	31972.0
32	40502.0
33	53234.0
34	75343.0
35	117105.0
36	222257.0
37	513000.0
38	940225.0
39	466654.0
40	1851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.186849516858686	19.64020152731929	13.381296825180033	23.791652130641992
2	15.866999623712926	19.99175209143396	39.9670843833263	24.174163901526814
3	18.504028886354998	25.099771949828963	29.90954009882174	26.4866590649943
4	12.411212196768368	16.567515218594355	38.51482634686841	32.50644623776887
5	12.871535968438359	37.681804148092716	35.604868085054605	13.841791798414313
6	31.3898115429246	37.94265063776203	17.329552257186155	13.337985562127209
7	27.59360971845815	32.37670428413414	22.246519212275786	17.783166785131915
8	25.49849087283081	36.00924518555936	20.03632851651716	18.455935425092672
9	25.27325255008474	16.199789356938126	21.007901585372206	37.51905650760493
10-11	24.196556578745323	26.430622260534825	28.67770586562919	20.695115295090666
12-13	25.15197643481566	23.692759407069556	28.91822500950209	22.237039148612695
14-15	22.122255912443855	25.539933048207004	26.39409577546159	25.943715263887558
16-17	21.468129988597493	29.135138730520715	26.888749524895477	22.507981755986318
18-19	21.42304256201025	27.691922053108016	29.463684197223373	21.421351187658363
20-21	22.384055492208287	26.99526795895097	29.23348536678069	21.387191182060054
22-23	21.923509488622734	26.77593592989415	29.315452378626627	21.98510220285649
24-25	21.792005084004277	27.004876858468478	29.278197580612975	21.924920476914263
26-27	21.59918858299635	26.974598177922598	29.305901701778787	22.12031153730226
28-29	21.6819382561632	27.066663144232468	29.36816843368058	21.883230165923756
30-31	21.943723075402183	27.472310201784772	28.949517686110042	21.634449036703003
32-33	21.55142900429436	27.103929364993217	29.439889321051638	21.90475230966078
34-35	21.64192312071886	26.93738476462661	29.491549073279007	21.929143041375525
36-37	22.078381016559945	27.20459796771652	28.848198441808275	21.86882257391526
38-39	21.94878076691603	27.42900907538719	28.938760240748113	21.683449916948668
40-41	21.810361314130873	27.186349518601418	29.074265374319097	21.929023792948612
42-43	21.726845825375005	27.1392253859536	28.995980790520566	22.13794799815083
44-45	21.935824430831357	27.1983260299267	28.80483471389357	22.061014825348373
46-47	22.026135554309935	27.444308794970794	28.499865712723306	22.029689937995965
48-49	22.089025734595356	27.43893260348956	28.273444330406356	22.198597331508722
50-51	22.102886831023874	27.457242621373023	28.13892413411414	22.30094641348896
52-53	22.1443174278697	27.43329951025269	28.138642828458444	22.283740233419167
54-55	22.175938648735045	27.804201099022645	27.978382162946815	22.041478089295495
56-57	21.977895628062726	27.49365005307064	28.098721838591146	22.42973248027549
58-59	21.858121122270706	27.19349274098388	28.462473624266643	22.485912512478766
60-61	22.058212876069256	27.53077131101074	28.154796983559176	22.25621882936083
62-63	21.945563123359555	27.53260174868028	28.081907884796987	22.439927243163176
64-65	21.88598772633968	27.243812379592907	28.314065981139287	22.55613391292813
66-67	21.836278745898873	27.30330173045522	28.17189310159241	22.6885264220535
68-69	22.198158395053376	27.445318563599834	28.127653493051604	22.228869548295187
70-71	22.183491524856823	27.314263691054464	28.080553769154402	22.421691014934314
72-73	22.092084973563114	27.41336603087135	28.108942551130127	22.385606444435407
74-75	22.048928443149617	27.45713107448351	27.97294549379795	22.520994988568923
76-77	22.039297769774027	27.517446861237353	27.833544724056242	22.60971064493238
78-79	22.247523136178145	27.58483138625887	27.88680799323907	22.280837484323918
80-81	22.157945358373183	27.382235399401626	28.00648959826559	22.453329643959595
82-83	22.020316353121114	27.415332058271737	28.12772711654651	22.436624472060643
84-85	21.95246962076267	27.39207393715904	27.919574791399892	22.735881650678394
86-87	22.0555109273277	27.534691716500465	27.867398480917206	22.542398875254634
88-89	21.808437856328393	27.3825351577347	27.974781451919423	22.834245534017484
90-91	21.82077986346631	27.431253299602165	27.959873796987377	22.788093039944144
92-93	21.965078664035982	27.56658212336835	27.813047439394694	22.65529177320098
94-95	21.7576776890916	27.287685290763967	27.936012922843023	23.01862409730141
96-97	21.7500950209046	27.291353097681494	27.957791714177123	23.00076016723679
98-99	21.641182060053215	27.408342835423795	27.853705815279362	23.096769289243635
100	21.757316609654122	27.342493348536678	27.789623717217786	23.11056632459141
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	26.0
2	82.5
3	179.0
4	134.5
5	94.0
6	219.5
7	511.0
8	928.0
9	1157.0
10	968.0
11	797.0
12	841.5
13	951.5
14	1146.0
15	1316.0
16	1506.5
17	1757.0
18	2016.5
19	2427.5
20	3050.5
21	3775.0
22	4830.0
23	6693.5
24	9140.5
25	12032.5
26	15944.5
27	21027.0
28	27100.0
29	33727.5
30	40280.0
31	46810.0
32	53639.0
33	61131.0
34	67867.0
35	73280.0
36	77790.5
37	82079.5
38	85931.0
39	87675.0
40	89506.5
41	91639.5
42	93115.0
43	94292.5
44	92977.0
45	91723.5
46	90462.5
47	86974.5
48	85281.5
49	84505.5
50	82018.0
51	78966.0
52	75117.5
53	71596.5
54	69680.0
55	67478.5
56	62524.5
57	57683.0
58	52982.0
59	45988.5
60	39706.0
61	34848.0
62	30422.5
63	25505.0
64	20656.0
65	17211.5
66	14424.0
67	11710.5
68	9602.0
69	7746.0
70	5905.0
71	4405.0
72	3416.5
73	2709.0
74	2137.5
75	1606.5
76	1087.5
77	806.0
78	628.0
79	431.0
80	320.0
81	181.0
82	73.5
83	33.5
84	15.0
85	4.5
86	6.0
87	6.5
88	1.5
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09399467882934245
2	0.0011402508551881414
3	0.0
4	3.040668947168377E-4
5	0.036944127708095784
6	1.1402508551881414E-4
7	0.0049410870391486126
8	3.4207525655644244E-4
9	3.4207525655644244E-4
10-11	4.5610034207525655E-4
12-13	0.0
14-15	9.502090459901178E-5
16-17	0.0
18-19	1.3302926643861648E-4
20-21	0.0
22-23	2.6605853287723296E-4
24-25	1.1402508551881414E-4
26-27	0.0017863930064614217
28-29	0.009255036107943749
30-31	0.03686811098441657
32-33	0.02620676548840745
34-35	0.0298555682250095
36-37	0.026890916001520337
38-39	0.026567844925883693
40-41	0.03234511592550361
42-43	0.02402128468263018
44-45	0.02305207145572026
46-47	0.016932725199543898
48-49	0.011402508551881414
50-51	0.008589889775750665
52-53	0.005834283542379323
54-55	0.004446978335233751
56-57	0.002755606233371342
58-59	0.0011592550361079438
60-61	7.031546940326871E-4
62-63	0.0016913721018624098
64-65	0.0030976814899277842
66-67	6.081337894336754E-4
68-69	0.0012922843025465602
70-71	4.5610034207525655E-4
72-73	6.461421512732801E-4
74-75	5.701254275940707E-4
76-77	4.1809198023565186E-4
78-79	5.701254275940707E-5
80-81	7.791714177118967E-4
82-83	6.081337894336754E-4
84-85	3.4207525655644244E-4
86-87	1.7103762827822122E-4
88-89	0.0
90-91	3.800836183960471E-5
92-93	3.800836183960471E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2631000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.12712295220797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99952983972366	50.79884970086097
2	13.825358886162626	18.008116960750286
3	3.995397166945018	7.806261676035947
4	1.5846368958693478	4.128113678075528
5	0.7851823354512266	2.556833325041692
6	0.46994918188380946	1.836386288990184
7	0.2812410428129199	1.2821493982138956
8	0.19754037989023043	1.0292189287309528
9	0.13543121658552074	0.7938220944719087
>10	0.669650862469434	7.684154924271438
>50	0.03989491603325243	1.7988185453407908
>100	0.015603327473504935	1.7255222003066082
>500	3.503692196641257E-4	0.1313206933673334
>1k	2.3357947972210284E-4	0.42043158554234833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4383	0.16659064994298747	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3739	0.14211326491828202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.800836183960471E-5	0.0	0.0	0.0
4	0.0	3.800836183960471E-5	0.0	0.0	0.0
5	0.0	1.1402508551881414E-4	0.0	0.0	0.0
6	0.0	1.1402508551881414E-4	0.0	0.0	0.0
7	0.0	1.1402508551881414E-4	0.0	0.0	0.0
8	0.0	1.1402508551881414E-4	0.0	0.0	0.0
9	0.0	1.1402508551881414E-4	0.0	0.0	0.0
10-11	0.0	1.1402508551881414E-4	0.0	3.800836183960471E-5	0.0
12-13	0.0	1.1402508551881414E-4	0.0	3.800836183960471E-5	0.0
14-15	0.0	1.1402508551881414E-4	0.0	3.800836183960471E-5	0.0
16-17	0.0	1.1402508551881414E-4	0.0	3.800836183960471E-5	0.0
18-19	0.0	1.330292664386165E-4	0.0	3.800836183960471E-5	0.0
20-21	0.0	1.5203344735841885E-4	0.0	9.502090459901178E-5	0.0
22-23	0.0	1.5203344735841885E-4	0.0	2.2805017103762827E-4	0.0
24-25	0.0	1.5203344735841885E-4	0.0	3.040668947168377E-4	0.0
26-27	0.0	1.5203344735841885E-4	0.0	4.1809198023565186E-4	0.0
28-29	0.0	1.5203344735841885E-4	0.0	0.0010832383124287344	0.0
30-31	0.0	1.5203344735841885E-4	0.0	0.004009882174078297	0.0
32-33	0.0	1.5203344735841885E-4	0.0	0.011554541999239833	0.0
34-35	0.0	1.5203344735841885E-4	0.0	0.02200684150513113	0.0
36-37	0.0	1.5203344735841885E-4	0.0	0.041885214747244395	0.0
38-39	0.0	3.040668947168377E-4	0.0	0.07563664006081339	0.0
40-41	0.0	3.040668947168377E-4	0.0	0.11499429874572406	0.0
42-43	0.0	3.040668947168377E-4	0.0	0.15682250095020905	0.0
44-45	0.0	3.420752565564424E-4	0.0	0.20370581527936146	0.0
46-47	0.0	4.5610034207525655E-4	0.0	0.2612884834663626	0.0
48-49	0.0	5.32117065754466E-4	0.0	0.32375522614975294	0.0
50-51	0.0	5.32117065754466E-4	0.0	0.3950779171417712	0.0
52-53	0.0	5.32117065754466E-4	0.0	0.4667996959331053	0.0
54-55	0.0	5.511212466742684E-4	0.0	0.5406879513492968	0.0
56-57	0.0	6.081337894336754E-4	0.0	0.6308817939946789	0.0
58-59	0.0	6.461421512732801E-4	0.0	0.7378183200304067	0.0
60-61	0.0	6.461421512732801E-4	0.0	0.8474344355758268	0.0
62-63	0.0	6.461421512732801E-4	0.0	0.9613074876472825	0.0
64-65	0.0	6.461421512732801E-4	0.0	1.0813949068795135	0.0
66-67	3.800836183960471E-5	6.461421512732801E-4	0.0	1.2190041809198022	0.0
68-69	3.800836183960471E-5	6.461421512732801E-4	0.0	1.3774230330672748	0.0
70-71	3.800836183960471E-5	6.651463321930824E-4	0.0	1.5457620676548842	0.0
72-73	3.800836183960471E-5	7.031546940326872E-4	0.0	1.7267578867350817	0.0
74-75	3.800836183960471E-5	7.411630558722919E-4	0.0	1.912200684150513	0.0
76-77	3.800836183960471E-5	7.981755986316989E-4	0.0	2.1065184340554923	0.0
78-79	3.800836183960471E-5	8.361839604713037E-4	0.0	2.3304066894716837	0.0
80-81	3.800836183960471E-5	9.122006841505131E-4	0.0	2.5835993918662106	0.0
82-83	3.800836183960471E-5	9.502090459901178E-4	0.0	2.8477385024705435	0.0
84-85	3.800836183960471E-5	9.502090459901178E-4	0.0	3.103781832003041	0.0
86-87	3.800836183960471E-5	9.502090459901178E-4	0.0	3.3816799695933106	0.0
88	3.800836183960471E-5	9.502090459901178E-4	0.0	3.6083998479665524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3985	0.0	50.41627	1
GTATCAA	6700	0.0	40.379807	1
TCAACGC	7780	0.0	34.265137	4
ATCAACG	7805	0.0	34.215626	3
CAACGCA	8025	0.0	33.219036	5
AACGCAG	8180	0.0	32.57348	6
TATCAAC	8275	0.0	32.38589	2
ACGCAGA	9500	0.0	27.899078	7
CGCAGAG	9805	0.0	26.983307	8
GTACATG	7305	0.0	26.21473	1
TACATGG	7610	0.0	24.77472	2
ACATGGG	7440	0.0	24.26651	3
GCAGAGT	11020	0.0	23.624046	9
GTGGTAT	1820	0.0	22.491533	1
GAGTACT	7225	0.0	21.658707	12-13
CAGAGTA	10785	0.0	20.456982	10-11
GTACTTT	7945	0.0	19.755075	14-15
AGAGTAC	10105	0.0	19.485153	10-11
CATGGGG	5055	0.0	19.25299	4
CGCGCGT	125	0.006418732	18.79725	7
>>END_MODULE