FastQCFastQC Report
Wed 25 May 2016
SRR1033103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161281
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.038.610351
TCAACGC26900.033.8822364
GTATCAA28800.033.840481
ATCAACG27250.033.6225743
CAACGCA28700.031.5935235
AACGCAG31000.029.3908336
GTACATG62550.027.248331
TACATGG63600.025.942032
TATCAAC36800.025.5467762
ACGCAGA35950.024.9520577
ACATGGG66350.023.5812153
CGCAGAG37850.023.4513458
CATGGGG41850.020.8804634
TAGCGTG1909.0788035E-619.7814925
GCAGAGT44750.019.4155869
GTATAGG7650.019.0791441
GAGTACA44950.018.2254491
TAGGACG1952.4756519E-416.8649884
CAGAGTA42700.016.55352610-11
AGAGTAC39200.016.23435810-11
Produced by FastQC (version 0.11.5)