##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033103_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2161281
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515252297133042	33.0	31.0	34.0	30.0	34.0
2	31.6823522716389	34.0	31.0	34.0	30.0	34.0
3	31.7648302094915	34.0	31.0	34.0	30.0	34.0
4	35.13551546513387	37.0	35.0	37.0	33.0	37.0
5	35.23363458985666	37.0	35.0	37.0	33.0	37.0
6	35.26555408574822	37.0	36.0	37.0	33.0	37.0
7	35.23204571733153	37.0	36.0	37.0	33.0	37.0
8	35.236626796793196	37.0	36.0	37.0	33.0	37.0
9	36.87193289535234	39.0	38.0	39.0	34.0	39.0
10-11	36.87198795529133	39.0	38.0	39.0	34.0	39.0
12-13	36.83130166785347	39.0	38.0	39.0	34.0	39.0
14-15	38.275972444119944	41.0	39.0	41.0	34.0	41.0
16-17	38.273766113707566	41.0	39.0	41.0	34.0	41.0
18-19	38.25459391906929	41.0	39.0	41.0	34.0	41.0
20-21	38.28587120323549	41.0	39.0	41.0	34.0	41.0
22-23	38.242976271942425	40.5	39.0	41.0	34.0	41.0
24-25	38.23385899380969	40.5	39.0	41.0	34.0	41.0
26-27	38.19893896258746	40.0	39.0	41.0	33.0	41.0
28-29	38.17047898908101	40.0	38.5	41.0	33.0	41.0
30-31	38.169670209473	40.0	38.0	41.0	33.0	41.0
32-33	38.236819969268225	41.0	39.0	41.0	33.5	41.0
34-35	38.20637436779391	41.0	39.0	41.0	33.0	41.0
36-37	38.12293264966471	41.0	38.0	41.0	33.0	41.0
38-39	38.037193682820515	41.0	38.0	41.0	33.0	41.0
40-41	37.96649834056747	40.0	38.0	41.0	33.0	41.0
42-43	37.84512842152409	40.0	38.0	41.0	33.0	41.0
44-45	37.70435588893808	40.0	38.0	41.0	32.0	41.0
46-47	37.577272691519525	40.0	38.0	41.0	32.0	41.0
48-49	37.45426254152052	40.0	38.0	41.0	32.0	41.0
50-51	37.32306928159735	40.0	37.0	41.0	31.0	41.0
52-53	37.17630562615412	40.0	37.0	41.0	31.0	41.0
54-55	37.019073410630085	40.0	36.5	41.0	31.0	41.0
56-57	36.86278184095451	40.0	36.0	41.0	31.0	41.0
58-59	36.66679945828423	40.0	36.0	41.0	30.5	41.0
60-61	36.44473971686236	39.0	35.0	41.0	30.5	41.0
62-63	36.18550387478537	39.0	35.0	41.0	30.0	41.0
64-65	35.915421224727375	39.0	35.0	41.0	30.0	41.0
66-67	35.62598824493437	38.0	35.0	40.0	30.0	41.0
68-69	35.32107139238257	37.0	35.0	40.0	29.0	41.0
70-71	34.979775882913884	37.0	34.5	40.0	29.0	41.0
72-73	34.62046096736148	36.0	34.0	39.0	29.0	41.0
74-75	34.25035245301282	36.0	34.0	39.0	28.5	41.0
76-77	33.92306090693436	35.5	34.0	38.5	28.0	40.0
78-79	33.55677188667276	35.0	34.0	37.0	28.0	39.0
80-81	33.19388756020157	35.0	34.0	37.0	27.0	39.0
82-83	32.97437144915446	35.0	34.0	36.5	27.5	39.0
84-85	32.69942386020142	35.0	34.0	36.0	27.0	37.5
86-87	32.443494390595205	35.0	34.0	36.0	27.0	37.0
88-89	32.08436964004218	35.0	33.0	35.0	26.0	37.0
90-91	31.891288314661537	35.0	33.0	35.0	26.0	36.0
92-93	31.710336601302654	35.0	33.0	35.0	25.5	36.0
94-95	31.53550163074584	35.0	33.0	35.0	25.0	36.0
96-97	31.354268140052127	35.0	33.0	35.0	25.0	36.0
98-99	31.15715610325543	35.0	33.0	35.0	24.0	35.0
100	30.98711504889924	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2613682054582682
1101	2	0.2626485546322357
1101	3	0.27851050847145586
1101	4	0.2114449110169616
1101	5	0.20509414307483098
1101	6	0.2730169850181099
1101	7	0.26159721121234725
1101	8	0.1963852948082021
1101	9	0.2443258985943899
1101	10-11	0.2767068870681513
1101	12-13	0.2765878025496704
1101	14-15	0.36307425246525327
1101	16-17	0.25599476147576894
1101	18-19	0.28597960486636964
1101	20-21	0.2566327726699882
1101	22-23	0.23183899197920965
1101	24-25	0.17671753466149198
1101	26-27	0.24994743472807812
1101	28-29	0.3192128364281146
1101	30-31	0.3590363405481938
1101	32-33	0.38063922646381343
1101	34-35	0.41128928068424386
1101	36-37	0.3150182439465752
1101	38-39	0.31624600529860203
1101	40-41	0.2903419647163261
1101	42-43	0.3077267641854391
1101	44-45	0.24670705840185292
1101	46-47	0.29668392381300634
1101	48-49	0.24943580656336906
1101	50-51	0.18101772865916388
1101	52-53	0.17080246148186973
1101	54-55	0.15900313751389916
1101	56-57	0.1988094998699097
1101	58-59	0.20775263548481604
1101	60-61	0.22266127657368884
1101	62-63	0.16278257852538047
1101	64-65	0.15578306338129266
1101	66-67	0.1876822387687227
1101	68-69	0.14029297388355388
1101	70-71	0.217254188051335
1101	72-73	0.22406006658130195
1101	74-75	0.21208720864053987
1101	76-77	0.05805449590347678
1101	78-79	0.0864064778524849
1101	80-81	0.18132741054790813
1101	82-83	0.20951685569802692
1101	84-85	0.20963285476874916
1101	86-87	0.16167778555205814
1101	88-89	0.3957149396368109
1101	90-91	0.37201480700634804
1101	92-93	0.35931631414302956
1101	94-95	0.40926345473241454
1101	96-97	0.4934367066396419
1101	98-99	0.5142974340964415
1101	100	0.525187124516421
1102	1	0.06125893452260556
1102	2	0.010623904840176834
1102	3	0.013122131436961837
1102	4	-0.006176055154483606
1102	5	-0.057315681355987635
1102	6	-0.074116870796189
1102	7	-0.0597929531051733
1102	8	-0.04702242210001373
1102	9	0.06648809993852467
1102	10-11	0.017204426849261267
1102	12-13	0.0370660738618227
1102	14-15	0.12651279243245028
1102	16-17	0.12707258401976418
1102	18-19	0.05961198714587823
1102	20-21	0.023331589514597795
1102	22-23	0.07641352774002286
1102	24-25	-0.03594420796109432
1102	26-27	0.012105881912347627
1102	28-29	0.02163075339951348
1102	30-31	-0.026234045513596982
1102	32-33	0.02505023448278365
1102	34-35	0.043354133525504324
1102	36-37	-0.021353506595445992
1102	38-39	-0.05669375071347105
1102	40-41	0.004539883591853311
1102	42-43	0.056046371935238426
1102	44-45	-0.024652867077527674
1102	46-47	-0.07805025570290525
1102	48-49	-0.04144942866125234
1102	50-51	-0.05750768711133958
1102	52-53	-0.1512187695363636
1102	54-55	-0.12662313641211398
1102	56-57	-0.10894067647823391
1102	58-59	-0.047885528417261014
1102	60-61	-0.10552299749945604
1102	62-63	-0.0770744821080882
1102	64-65	-0.0055471966618938495
1102	66-67	-0.07860562198764853
1102	68-69	-0.09049119113502968
1102	70-71	-0.06903009164764029
1102	72-73	0.08006780426181592
1102	74-75	-0.06134897280547591
1102	76-77	-0.13681896618924583
1102	78-79	-0.06773878541473977
1102	80-81	0.02407019758426543
1102	82-83	0.024813272044092116
1102	84-85	-0.04289135793957399
1102	86-87	-0.08330792267506126
1102	88-89	0.05085840912966688
1102	90-91	0.06785564144681189
1102	92-93	0.09517503223266743
1102	94-95	0.11188249513886106
1102	96-97	0.13877695036792304
1102	98-99	0.16449655603414826
1102	100	0.10337805221584873
1103	1	0.22269840821776654
1103	2	0.18869744936678146
1103	3	0.23920330755017716
1103	4	0.238376764662668
1103	5	0.21134144652094022
1103	6	0.19881700404412328
1103	7	0.1620842965276026
1103	8	0.17981931194293566
1103	9	0.1977656416854714
1103	10-11	0.24301471076592662
1103	12-13	0.337697999609901
1103	14-15	0.34019893407036506
1103	16-17	0.31168270315407653
1103	18-19	0.31019704771171064
1103	20-21	0.26065161889245303
1103	22-23	0.2585739658280488
1103	24-25	0.23114150590849647
1103	26-27	0.2516965579777164
1103	28-29	0.2752077995510618
1103	30-31	0.18456720866318221
1103	32-33	0.20112157983080436
1103	34-35	0.25651362702784297
1103	36-37	0.21874717292369183
1103	38-39	0.28667688057770846
1103	40-41	0.32107923578083586
1103	42-43	0.3180525970462398
1103	44-45	0.20925708311325764
1103	46-47	0.23993785999427075
1103	48-49	0.22590114370773762
1103	50-51	0.12245976213278453
1103	52-53	0.11223616706819683
1103	54-55	0.15006624677759106
1103	56-57	0.17520271537502907
1103	58-59	0.1781517473476626
1103	60-61	0.20792481310071054
1103	62-63	0.2235178605060355
1103	64-65	0.2161046735761687
1103	66-67	0.23694633646722707
1103	68-69	0.19061631478422925
1103	70-71	0.1831460444559312
1103	72-73	0.30127063219325123
1103	74-75	0.29828356463465866
1103	76-77	0.23866955522206013
1103	78-79	0.24824770326480206
1103	80-81	0.30256911776052675
1103	82-83	0.25697225372245924
1103	84-85	0.2232302127358281
1103	86-87	0.305614053298342
1103	88-89	0.39635586665452394
1103	90-91	0.29603004234229857
1103	92-93	0.3620474341018678
1103	94-95	0.41628002007467657
1103	96-97	0.36708461274719895
1103	98-99	0.4523390203625155
1103	100	0.4924865006402186
1104	1	-0.08451670941167677
1104	2	-0.07724507226392774
1104	3	-0.14824242468679927
1104	4	-0.07939141392225224
1104	5	-0.16301093825521207
1104	6	-0.24293557176786607
1104	7	-0.14660246873905436
1104	8	-0.15466818364095758
1104	9	-0.15886202208298528
1104	10-11	-0.14414868337070885
1104	12-13	-0.10920700700756925
1104	14-15	-0.16398828266494547
1104	16-17	-0.1716383210650818
1104	18-19	-0.23467262342768436
1104	20-21	-0.16039708737980618
1104	22-23	-0.1725849556777277
1104	24-25	-0.2194872977010789
1104	26-27	-0.24349295346694078
1104	28-29	-0.18274863334530522
1104	30-31	-0.19151238536700532
1104	32-33	-0.19344619276009922
1104	34-35	-0.300172390312639
1104	36-37	-0.15638379736228103
1104	38-39	-0.30193205034269965
1104	40-41	-0.3673543419496781
1104	42-43	-0.30368877802654026
1104	44-45	-0.32300000703232
1104	46-47	-0.31658432157408356
1104	48-49	-0.32173064021017694
1104	50-51	-0.38211745332875324
1104	52-53	-0.44982032536172056
1104	54-55	-0.48957780450134436
1104	56-57	-0.3804138562729449
1104	58-59	-0.4523870388677764
1104	60-61	-0.48161603021430466
1104	62-63	-0.4647548061906832
1104	64-65	-0.49639818938965874
1104	66-67	-0.44476826010495785
1104	68-69	-0.4688497974327319
1104	70-71	-0.3914423465315622
1104	72-73	-0.32626137175574854
1104	74-75	-0.47089644060364577
1104	76-77	-0.6601515432753686
1104	78-79	-0.47888903742901334
1104	80-81	-0.4836458745085821
1104	82-83	-0.4113543532170212
1104	84-85	-0.37027225922273743
1104	86-87	-0.254879721194456
1104	88-89	-0.17808618656422226
1104	90-91	-0.2487965645467547
1104	92-93	-0.30257594986675684
1104	94-95	-0.25340003473290196
1104	96-97	-0.19841278388271633
1104	98-99	-0.2202884142678201
1104	100	-0.1795508932485106
1105	1	-0.08134074935687607
1105	2	-0.014784689075320046
1105	3	-0.044076128270706505
1105	4	-0.09436553192614383
1105	5	-0.02588898383769589
1105	6	-0.07804719392350279
1105	7	-0.07133990677885294
1105	8	-0.009740162327169344
1105	9	-0.10147821856104144
1105	10-11	-0.02094227588339237
1105	12-13	-0.04587910204131873
1105	14-15	0.015155877703513454
1105	16-17	-0.03326447540763411
1105	18-19	-0.018862894308924183
1105	20-21	-0.006440974809414968
1105	22-23	0.07051071035665046
1105	24-25	0.005614437283242069
1105	26-27	0.11165105129754238
1105	28-29	0.03414114138042379
1105	30-31	0.014431844430838225
1105	32-33	0.07375051475458605
1105	34-35	-0.01789503136230053
1105	36-37	0.035974704716458916
1105	38-39	0.037541123571593005
1105	40-41	-0.07008227906355557
1105	42-43	-0.10910867377187827
1105	44-45	-0.014140434090194276
1105	46-47	-0.03318599198669858
1105	48-49	-0.0028800776860080646
1105	50-51	-0.08929022389701657
1105	52-53	-0.11001066772600865
1105	54-55	-0.09451999801976996
1105	56-57	-0.06219415135968376
1105	58-59	-0.10625661153739685
1105	60-61	-0.1741551652062867
1105	62-63	-0.07098794004287612
1105	64-65	-0.07351738519356843
1105	66-67	-0.09993968315860258
1105	68-69	-0.12759033728374192
1105	70-71	-0.09772760179119189
1105	72-73	-0.22724777852428701
1105	74-75	-0.2777234859961695
1105	76-77	-0.26099526040945165
1105	78-79	-0.2452188649337117
1105	80-81	-0.14970972387445158
1105	82-83	-0.13891149127289282
1105	84-85	-0.17654373128767276
1105	86-87	-0.11649141108126315
1105	88-89	0.0580242827792361
1105	90-91	0.02822753116312171
1105	92-93	0.0588509109336357
1105	94-95	4.100905045980596E-4
1105	96-97	-0.010221526061542363
1105	98-99	0.037220366101124114
1105	100	0.1720426059807778
1106	1	0.1817715797720929
1106	2	0.13974215501105647
1106	3	0.1094789325306067
1106	4	0.2213837534579497
1106	5	0.13428035418741757
1106	6	0.17826180316143336
1106	7	0.16909631552093884
1106	8	0.18486753005309708
1106	9	0.1318298634381847
1106	10-11	0.10650722930996182
1106	12-13	0.18203360632935528
1106	14-15	0.21986720118159297
1106	16-17	0.19066110076617093
1106	18-19	0.27759558249789507
1106	20-21	0.1848028813806195
1106	22-23	0.19821120374822243
1106	24-25	0.16696249060349544
1106	26-27	0.200771295446188
1106	28-29	0.27101216146513707
1106	30-31	0.22402911238114598
1106	32-33	0.20546889476987218
1106	34-35	0.20362070905523666
1106	36-37	0.18698894043121328
1106	38-39	0.22163844340820305
1106	40-41	0.22018069015371822
1106	42-43	0.18063283810065656
1106	44-45	0.19215677065316328
1106	46-47	0.1853058618637391
1106	48-49	0.22150511679328844
1106	50-51	0.18650632519214838
1106	52-53	0.12772859888468702
1106	54-55	0.16396298798979814
1106	56-57	0.1562148259141125
1106	58-59	0.12471870945014274
1106	60-61	0.16559969559692433
1106	62-63	0.21345866895137533
1106	64-65	0.18124535289381072
1106	66-67	0.16969239578406103
1106	68-69	0.18372446163325407
1106	70-71	0.17613566364969557
1106	72-73	0.21433984800754047
1106	74-75	0.17028294790539888
1106	76-77	0.1954195847335214
1106	78-79	0.1804327798620946
1106	80-81	0.1399792884933504
1106	82-83	0.11140930758749334
1106	84-85	0.15307070631197206
1106	86-87	0.15784552913628147
1106	88-89	0.22843701473458822
1106	90-91	0.23485666673731131
1106	92-93	0.256162728746677
1106	94-95	0.25092555733981925
1106	96-97	0.1850363488641662
1106	98-99	0.22719819649894646
1106	100	0.2361770952780482
1107	1	0.1990246222882952
1107	2	0.1396789338952047
1107	3	0.1255650938799242
1107	4	0.22233297335453983
1107	5	0.153236754196449
1107	6	0.0850910384117185
1107	7	0.12343215511993577
1107	8	0.10014130006370436
1107	9	0.08524809369852449
1107	10-11	0.08729139693623011
1107	12-13	0.05856728286303792
1107	14-15	0.03066288408254536
1107	16-17	0.06380477075195046
1107	18-19	0.17060219813608768
1107	20-21	0.10807818676119041
1107	22-23	0.1610299623564586
1107	24-25	0.13585047633358727
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2216	7	0.03281131845593421
2216	8	0.04869849034751894
2216	9	-0.028319430660367573
2216	10-11	-0.014338650377268891
2216	12-13	0.031160019484545387
2216	14-15	-0.0743806486588312
2216	16-17	-0.027202783564952426
2216	18-19	-0.008082601771732811
2216	20-21	-0.06044645298646856
2216	22-23	-0.05146598683014503
2216	24-25	-0.017294755228917325
2216	26-27	-0.08084309224166475
2216	28-29	-0.026713687930531194
2216	30-31	0.02341671007241075
2216	32-33	0.051705749897294595
2216	34-35	0.05451420979349564
2216	36-37	0.08156182347816099
2216	38-39	0.05964573181307031
2216	40-41	0.11695207505081129
2216	42-43	0.10370919405848866
2216	44-45	0.09997379522899053
2216	46-47	0.14412473157999273
2216	48-49	0.17832899102723587
2216	50-51	0.1844747875150219
2216	52-53	0.19441329623958126
2216	54-55	0.1408247667372322
2216	56-57	0.13468983037169124
2216	58-59	0.08177109260626736
2216	60-61	0.23053417407929544
2216	62-63	0.1970156829892744
2216	64-65	0.18884624962004182
2216	66-67	0.21204151398323035
2216	68-69	0.23915716809965915
2216	70-71	0.23550727413073957
2216	72-73	0.2916073172401781
2216	74-75	0.25193568085687446
2216	76-77	0.1972811130547214
2216	78-79	0.25226503836606895
2216	80-81	0.2091924893612287
2216	82-83	0.28972255673755143
2216	84-85	0.2047676144496151
2216	86-87	0.23089634161912898
2216	88-89	0.2895959472377072
2216	90-91	0.33867340282212766
2216	92-93	0.349879234239026
2216	94-95	0.33543767696742677
2216	96-97	0.37735323219960293
2216	98-99	0.33991271661217937
2216	100	0.3891801464711797
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26011.0
3	4272.0
4	2772.0
5	2217.0
6	2003.0
7	2196.0
8	2529.0
9	2691.0
10	3029.0
11	3243.0
12	3585.0
13	4092.0
14	4578.0
15	4889.0
16	4657.0
17	4686.0
18	4669.0
19	4693.0
20	4617.0
21	4924.0
22	5760.0
23	7678.0
24	11887.0
25	16161.0
26	16898.0
27	14644.0
28	15186.0
29	19138.0
30	23632.0
31	28991.0
32	37640.0
33	51938.0
34	72592.0
35	111621.0
36	209201.0
37	433631.0
38	691510.0
39	296225.0
40	595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78101751313973	19.901966154694602	13.454841204482133	23.862175127683535
2	15.267846720265041	20.023050141469316	40.89255372962036	23.816549408645287
3	18.164077962518768	25.458607817455963	29.757389138044623	26.619925081980643
4	12.173964562225782	16.710769867384908	39.200301046581224	31.91496452380809
5	12.885680997719367	38.05978336088266	35.82220057980889	13.232335061589072
6	30.732678380303103	38.428399622224354	17.847539483894792	12.99138251357776
7	27.298799383103077	32.54751314006179	22.555328463370472	17.59835901346466
8	24.89231617858022	36.85308724471947	20.40356212713262	17.851034449567692
9	24.853602530059263	17.266062535112717	21.181147837611956	36.69918709721606
10-11	23.70541653043369	27.10044633508429	28.99898441355957	20.195152720922447
12-13	24.290214323991385	24.396256433560673	28.976174191345038	22.337355051102904
14-15	21.658513694840757	26.489858128803302	25.945701924943602	25.905926251412335
16-17	21.49563980360547	29.80281643211054	26.4606663706418	22.24087739364219
18-19	21.314052170299355	28.897194202970983	28.333068393197074	21.45568523353258
20-21	22.578991760953095	28.137184006923533	27.83876875942238	21.445055472700997
22-23	22.212321748081806	27.81295646848159	27.899469284710925	22.07525249872568
24-25	22.04538338876613	27.988112320659113	27.83024860411404	22.13625568646072
26-27	21.7491173681675	27.984785736465966	27.960212266650373	22.305884628716157
28-29	21.816779599992966	27.9590274621887	28.011460241329615	22.212732696488715
30-31	21.995194004190495	28.477596122808578	27.60504294042717	21.922166932573752
32-33	21.718356322626388	28.124172727929075	28.12079421821285	22.036676731231683
34-35	21.881339774914853	27.852320265067377	28.195477935732267	22.070862024285503
36-37	22.21275936106021	28.24166620393507	27.635325855590725	21.910248579413995
38-39	21.992749632807865	28.326455325651068	27.756115935537075	21.924679106003996
40-41	21.77780564443193	28.051435262510438	28.066034407245805	22.10472468581183
42-43	21.797720522194854	27.954235533700523	28.039932275512246	22.208111668592377
44-45	21.88471296700327	28.124911792319907	27.96279475247016	22.027580488206663
46-47	21.87983185519872	28.30307154159187	27.899876614532275	21.917219988677132
48-49	21.90996626858351	28.10983763574697	27.859836478976863	22.120359616692657
50-51	21.88525282344871	28.028068486923	27.887355877735963	22.199322811892323
52-53	21.92940892304603	27.99606963601794	27.935800044698983	22.13872139623705
54-55	21.96462212478707	28.353660299938532	27.79170644955984	21.890011125714558
56-57	21.80539232470549	28.101211352847006	27.821739573018945	22.271656749428566
58-59	21.68860141757863	27.72447327794642	28.199436383284354	22.387488921190595
60-61	21.995897183900507	28.178068789146394	27.79827951944257	22.027754507510533
62-63	21.80368537300353	28.188969237044176	27.796852656567278	22.210492733385017
64-65	21.772253575151638	27.887478328383235	27.999566914688206	22.340701181776918
66-67	21.687865545611924	27.94305209721185	27.840240676815633	22.52884168036059
68-69	22.071820711960633	28.139611283467865	27.74180264342048	22.046765361151017
70-71	22.035592645494493	27.866708494641717	27.83098814426765	22.266710715596144
72-73	21.785245308475975	27.978015378223038	27.961959765652832	22.274779547648155
74-75	21.90722257670029	28.011273173650093	27.835424805331392	22.24607944431823
76-77	21.900208866024983	28.058887818492927	27.678935810482542	22.36196750499955
78-79	22.013645599836572	28.16582566382389	27.747032390890382	22.073496345449158
80-81	21.99284427802513	27.880085554024774	27.834902479285304	22.292167688664787
82-83	21.788020591018974	27.997345034675803	27.986864908396065	22.227769465909166
84-85	21.873599604297482	27.874077575183826	27.887657870938142	22.364664949580547
86-87	22.03623258313605	27.986543783023706	27.668646325023488	22.308577308816755
88-89	21.767366928817918	27.93522549887365	27.84664442752679	22.45076314478164
90-91	21.788116634983933	27.824882263355338	27.927437031581793	22.459564070078937
92-93	21.917226663091625	28.01948862038853	27.735999158376114	22.327285558143735
94-95	21.68625434983406	27.769075329945082	27.957578805506998	22.587091514713862
96-97	21.707884842147937	27.795651618431343	27.930370601706784	22.56609293771394
98-99	21.735307943274954	27.80425484382927	27.88514434913735	22.57529286375843
100	21.90197597920463	27.722259844407056	27.76400883384414	22.611755342544175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	299.0
1	282.5
2	246.0
3	210.0
4	179.0
5	143.0
6	123.0
7	139.0
8	139.5
9	137.5
10	158.5
11	166.0
12	177.5
13	179.0
14	205.5
15	272.0
16	339.5
17	452.5
18	648.5
19	945.5
20	1449.0
21	2207.5
22	3293.0
23	5198.0
24	7667.5
25	10386.5
26	14170.5
27	18650.5
28	24430.0
29	30824.0
30	36417.5
31	42375.0
32	48288.5
33	53587.5
34	59020.0
35	64217.0
36	68158.0
37	71376.5
38	74180.5
39	75654.5
40	77013.5
41	78036.5
42	77305.0
43	76449.5
44	75490.5
45	73682.0
46	72640.5
47	70846.0
48	68598.5
49	67532.5
50	65524.0
51	63034.0
52	59646.0
53	56178.5
54	53443.5
55	50962.5
56	47838.5
57	43631.5
58	39946.5
59	36448.0
60	32750.0
61	28898.5
62	24970.0
63	21053.0
64	17431.0
65	14316.5
66	11524.0
67	8957.5
68	6893.0
69	5492.0
70	4201.5
71	3137.0
72	2471.5
73	1945.5
74	1589.0
75	1198.5
76	876.5
77	725.5
78	571.0
79	408.5
80	275.0
81	154.0
82	88.0
83	62.0
84	44.0
85	29.0
86	24.0
87	23.0
88	21.0
89	15.5
90	7.0
91	6.0
92	3.5
93	3.0
94	4.5
95	3.5
96	2.0
97	1.5
98	2.0
99	2.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32975813880749427
2	0.11608856044170102
3	0.06107489030810895
4	0.037847924448509936
5	0.04228973465273604
6	0.009716459821744605
7	0.006570177593751113
8	0.015037378295557126
9	0.0177209719606104
10-11	0.021306808323397097
12-13	0.01605529313402561
14-15	0.019085903221284044
16-17	0.01973366720940035
18-19	0.01922470979016611
20-21	0.0246844348328607
22-23	0.015245588148880225
24-25	0.0257486185276232
26-27	0.019155306505725077
28-29	0.019386650787195186
30-31	0.020288893484928613
32-33	0.026141903806122385
34-35	0.014852302870381036
36-37	0.009300040115098407
38-39	0.013279161756384291
40-41	0.009045561405481286
42-43	0.007958243282571772
44-45	0.008698544983276121
46-47	0.007750033429248672
48-49	0.004210465922755995
50-51	0.006408236596722037
52-53	0.007310479294455464
54-55	0.0033082232250225677
56-57	0.0028224002339353373
58-59	0.0027992658057883267
60-61	0.0038171806442568092
62-63	0.0028455346620823484
64-65	0.0020358296769369647
66-67	0.001966426392495932
68-69	0.003053744515405447
70-71	0.0022209051021130524
72-73	0.001989560820642943
74-75	0.0020358296769369647
76-77	0.004025390497579907
78-79	0.0033082232250225677
80-81	0.0034007609376106116
82-83	0.0021977706739660418
84-85	0.0028455346620823484
86-87	0.002290308386554085
88-89	0.5999451251364353
90-91	0.6089212832574755
92-93	0.6039473812058682
94-95	0.5912928490094532
96-97	0.5892107504762222
98-99	0.5862726781015518
100	0.588817465197723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2161281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.24647731837693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51629520477296	56.559861048618245
2	12.353162661283823	17.355323213921896
3	3.552544255887032	7.48661158481096
4	1.407828621380085	3.95580005279752
5	0.6818506199278547	2.3948802053641645
6	0.39379897695150845	1.659779454145451
7	0.2539156823681408	1.2485677555577663
8	0.16688709109467986	0.9378584207449867
9	0.11055503205073698	0.6989491396246077
>10	0.5396989689230969	6.402119020941993
>50	0.019697887107671716	0.9157626471938536
>100	0.0037649982523986145	0.38448745627850783
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	9.253771258804385E-5	0.0	4.626885629402193E-5	0.0
8	0.0	9.253771258804385E-5	0.0	4.626885629402193E-5	0.0
9	0.0	9.253771258804385E-5	0.0	9.253771258804385E-5	0.0
10-11	0.0	9.253771258804385E-5	0.0	9.253771258804385E-5	0.0
12-13	0.0	1.3880656888206578E-4	0.0	9.253771258804385E-5	0.0
14-15	0.0	1.3880656888206578E-4	0.0	1.3880656888206578E-4	0.0
16-17	0.0	1.3880656888206578E-4	0.0	1.850754251760877E-4	0.0
18-19	0.0	1.3880656888206578E-4	0.0	1.850754251760877E-4	0.0
20-21	0.0	1.3880656888206578E-4	0.0	1.850754251760877E-4	0.0
22-23	0.0	1.3880656888206578E-4	0.0	2.0820985332309865E-4	0.0
24-25	0.0	1.6194099702907674E-4	0.0	2.7761313776413155E-4	0.0
26-27	0.0	1.850754251760877E-4	0.0	5.320918473812521E-4	0.0
28-29	0.0	1.850754251760877E-4	0.0	0.0016194099702907675	0.0
30-31	0.0	1.850754251760877E-4	0.0	0.004973902051607357	0.0
32-33	0.0	1.850754251760877E-4	0.0	0.01274706990900304	0.0
34-35	0.0	2.7761313776413155E-4	0.0	0.02331950357218705	0.0
36-37	0.0	2.7761313776413155E-4	0.0	0.04356212820082164	0.0
38-39	0.0	2.7761313776413155E-4	0.0	0.0770145113013995	0.0
40-41	0.0	2.7761313776413155E-4	0.0	0.11694453428314042	0.0
42-43	0.0	2.7761313776413155E-4	0.0	0.16110815761578434	0.0
44-45	0.0	3.701508503521754E-4	0.0	0.2101994141437416	0.0
46-47	0.0	3.701508503521754E-4	0.0	0.2671795106698296	0.0
48-49	0.0	3.701508503521754E-4	0.0	0.33302009317622283	0.0
50-51	0.0	4.164197066461973E-4	0.0	0.4058241385548663	0.0
52-53	0.0	5.089574192342412E-4	0.0	0.47798041994539353	0.0
54-55	0.0	5.089574192342412E-4	0.0	0.5580024069059044	0.0
56-57	0.0	5.552262755282631E-4	0.0	0.6531080410182665	0.0
58-59	0.0	5.783607036752741E-4	0.0	0.7588092432219595	0.0
60-61	0.0	6.01495131822285E-4	0.0	0.875614970936218	0.0
62-63	0.0	6.01495131822285E-4	0.0	0.9947110070370304	0.0
64-65	0.0	6.01495131822285E-4	0.0	1.1169533253658361	0.0
66-67	0.0	6.01495131822285E-4	0.0	1.257448707502634	0.0
68-69	0.0	6.01495131822285E-4	0.0	1.420130006232415	0.0
70-71	0.0	6.01495131822285E-4	0.0	1.595350165017876	0.0
72-73	0.0	6.940328444103289E-4	0.0	1.7752434782890332	0.0
74-75	0.0	6.940328444103289E-4	0.0	1.9593703919110936	0.0
76-77	0.0	6.940328444103289E-4	0.0	2.159899615089384	0.0
78-79	0.0	6.940328444103289E-4	0.0	2.3927244999609028	0.0
80-81	0.0	6.940328444103289E-4	0.0	2.6434091633619134	0.0
82-83	0.0	6.940328444103289E-4	0.0	2.916094667930732	0.0
84-85	0.0	6.940328444103289E-4	0.0	3.1813771554925063	0.0
86-87	0.0	6.940328444103289E-4	0.0	3.460725375367664	0.0
88	0.0	6.940328444103289E-4	0.0	3.6984547590063483	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	38.61035	1
TCAACGC	2690	0.0	33.882236	4
GTATCAA	2880	0.0	33.84048	1
ATCAACG	2725	0.0	33.622574	3
CAACGCA	2870	0.0	31.593523	5
AACGCAG	3100	0.0	29.390833	6
GTACATG	6255	0.0	27.24833	1
TACATGG	6360	0.0	25.94203	2
TATCAAC	3680	0.0	25.546776	2
ACGCAGA	3595	0.0	24.952057	7
ACATGGG	6635	0.0	23.581215	3
CGCAGAG	3785	0.0	23.451345	8
CATGGGG	4185	0.0	20.880463	4
TAGCGTG	190	9.0788035E-6	19.781492	5
GCAGAGT	4475	0.0	19.415586	9
GTATAGG	765	0.0	19.079144	1
GAGTACA	4495	0.0	18.225449	1
TAGGACG	195	2.4756519E-4	16.864988	4
CAGAGTA	4270	0.0	16.553526	10-11
AGAGTAC	3920	0.0	16.234358	10-11
>>END_MODULE