FastQCFastQC Report
Wed 25 May 2016
SRR1033103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161281
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41860.19368143244677577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33230.15375140946503485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.053.739561
GTATCAA53600.042.390941
TCAACGC65850.034.0527084
ATCAACG65950.034.0010723
CAACGCA66850.033.544875
AACGCAG69750.032.0731246
TATCAAC71650.031.8866672
ACGCAGA80350.027.607987
CGCAGAG81250.027.2430638
GTACATG62100.026.8922671
TACATGG62250.025.9026262
ACATGGG64900.024.2654973
GCAGAGT91600.023.8564519
GTGGTAT14800.022.5677011
TATACTG14200.021.850675
GAGTACT62900.020.88139212-13
AGAGTAC86150.020.4016210-11
TGGTATC15450.020.3861582
CAGAGTA90600.020.17761410-11
CATGGGG43450.019.1501664
Produced by FastQC (version 0.11.5)