FastQCFastQC Report
Wed 25 May 2016
SRR1033102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727758
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.036.857741
GTATCAA24050.035.444391
TCAACGC24250.033.1253434
ATCAACG24650.032.5944183
CAACGCA26200.030.659025
AACGCAG27000.029.3924316
GTACATG58650.027.8641261
TATCAAC29950.027.6252572
TACATGG59150.026.3860172
ACATGGG60900.024.4571593
ACGCAGA32650.024.1623217
CGCAGAG33900.023.132868
GTATAGA6500.021.012061
CATGGGG41250.020.8402614
GAGTACA41450.019.3156071
GCAGAGT40000.019.252919
ACCGTAC1508.342325E-418.7833298
TCTGTCG4600.018.3749968
TAGACTG6300.017.89515
TCTATAC7000.017.4517653
Produced by FastQC (version 0.11.5)