FastQCFastQC Report
Wed 25 May 2016
SRR1033102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727758
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44560.2579064892189763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.2125876424823384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.11639361530955145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.058.197151
GTATCAA54550.043.390371
TCAACGC65150.035.428024
ATCAACG65450.035.3374563
CAACGCA66600.034.656695
AACGCAG68250.034.0116846
TATCAAC69950.033.3329542
ACGCAGA79050.029.3649277
CGCAGAG80300.028.8492988
CCGATCG1005.3329215E-528.1938959
GTGGTAT12950.026.162721
GCAGAGT88350.026.0079549
GTACATG61350.025.1581921
TGGTATC12850.024.5104642
TACATGG61950.024.2822252
ACATGGG63200.022.9093883
GAGTACT60050.022.30158412-13
CAGAGTA87200.022.01300810-11
GTATAGA6450.021.1572111
AGAGTAC84200.020.8998710-11
Produced by FastQC (version 0.11.5)