FastQCFastQC Report
Wed 25 May 2016
SRR1033101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331266
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36900.034.0197871
TCAACGC37200.032.352744
ATCAACG38700.031.46323
GGTATCA26450.031.4625781
CAACGCA41500.029.1138135
AACGCAG42050.028.7252466
GTACATG95050.026.7108881
TATCAAC46450.026.517322
TACATGG95000.025.5847382
ACATGGG93000.024.6184163
ACGCAGA52150.022.5314357
CGCAGAG53800.021.6865438
ACACCGT6600.020.6514576
CATGGGG69750.020.3552674
TACACCG5550.019.482695
GAGTACA65050.019.2979281
TCTATAC11800.017.9285263
GTATATA24750.017.666661
ACCGTTC4600.017.3864968
AGTACAT66700.016.8455872
Produced by FastQC (version 0.11.5)