##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033101_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3331266
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675130716070107	33.0	31.0	34.0	30.0	34.0
2	31.800405911746466	34.0	31.0	34.0	30.0	34.0
3	31.868863969433843	34.0	31.0	34.0	30.0	34.0
4	35.248715953634445	37.0	35.0	37.0	33.0	37.0
5	35.28659824823355	37.0	35.0	37.0	33.0	37.0
6	35.360875114746165	37.0	36.0	37.0	33.0	37.0
7	35.264208562150245	37.0	36.0	37.0	33.0	37.0
8	35.24660894686885	37.0	35.0	37.0	33.0	37.0
9	36.904217795877	39.0	38.0	39.0	34.0	39.0
10-11	36.889325109432875	39.0	38.0	39.0	34.0	39.0
12-13	36.8238866244845	39.0	38.0	39.0	33.5	39.0
14-15	38.229479573231316	41.0	39.0	41.0	34.0	41.0
16-17	38.23491219254181	41.0	39.0	41.0	34.0	41.0
18-19	38.246045197231325	41.0	39.0	41.0	34.0	41.0
20-21	38.26689747981698	41.0	39.0	41.0	34.0	41.0
22-23	38.23544202114151	40.5	39.0	41.0	34.0	41.0
24-25	38.250949939152264	40.5	39.0	41.0	34.0	41.0
26-27	38.16103877624903	40.0	38.0	41.0	33.0	41.0
28-29	38.16993419318661	40.0	38.0	41.0	33.0	41.0
30-31	38.318266689000524	41.0	39.0	41.0	34.0	41.0
32-33	38.30439403518062	41.0	39.0	41.0	33.5	41.0
34-35	38.2585036739786	41.0	39.0	41.0	33.0	41.0
36-37	38.187367505326804	41.0	38.0	41.0	33.0	41.0
38-39	38.100933999266346	41.0	38.0	41.0	33.0	41.0
40-41	38.03235256506085	40.0	38.0	41.0	33.0	41.0
42-43	37.9183744258189	40.0	38.0	41.0	33.0	41.0
44-45	37.79372526841147	40.0	38.0	41.0	32.5	41.0
46-47	37.670638279861166	40.0	38.0	41.0	32.0	41.0
48-49	37.55710981951006	40.0	38.0	41.0	32.0	41.0
50-51	37.43489502189257	40.0	37.0	41.0	32.0	41.0
52-53	37.282215830257925	40.0	37.0	41.0	31.0	41.0
54-55	37.12523121840165	40.0	37.0	41.0	31.0	41.0
56-57	36.95375812078652	40.0	36.0	41.0	31.0	41.0
58-59	36.75850907732976	40.0	36.0	41.0	31.0	41.0
60-61	36.53903215774423	39.0	35.0	41.0	31.0	41.0
62-63	36.284523061202556	39.0	35.0	41.0	30.0	41.0
64-65	36.00832746469361	39.0	35.0	41.0	30.0	41.0
66-67	35.70765828967126	38.0	35.0	40.0	30.0	41.0
68-69	35.38889074003697	37.0	35.0	40.0	29.5	41.0
70-71	35.05159074658103	37.0	34.5	40.0	29.0	41.0
72-73	34.69935183800993	36.5	34.0	39.0	29.0	41.0
74-75	34.34378146326352	36.0	34.0	39.0	29.0	41.0
76-77	33.975752161490554	35.5	34.0	38.5	28.5	40.0
78-79	33.590830332972516	35.0	34.0	37.0	28.0	39.0
80-81	33.240692877722765	35.0	34.0	37.0	27.5	39.0
82-83	33.02025821414441	35.0	34.0	36.5	27.5	39.0
84-85	32.79714739081178	35.0	34.0	36.0	27.5	37.0
86-87	32.62257847316906	35.0	34.0	36.0	27.5	37.0
88-89	32.4100969421235	35.0	34.0	35.5	27.0	37.0
90-91	32.20680606111911	35.0	33.5	35.0	27.0	36.0
92-93	32.00292006102185	35.0	33.0	35.0	27.0	36.0
94-95	31.818942107895317	35.0	33.0	35.0	26.0	36.0
96-97	31.65830963363478	35.0	33.0	35.0	26.0	36.0
98-99	31.495373080384454	35.0	33.0	35.0	25.0	35.0
100	31.364809354761825	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06300947299755322
1101	2	0.11273346452265187
1101	3	0.1474749136996678
1101	4	0.09559892657076574
1101	5	0.09596688646394114
1101	6	0.18710768699536828
1101	7	0.14531294167471742
1101	8	0.20136006078954694
1101	9	0.23573665228331464
1101	10-11	0.20300173917549102
1101	12-13	0.20365035405872334
1101	14-15	0.2907748251984543
1101	16-17	0.2839339217600525
1101	18-19	0.306873712459911
1101	20-21	0.26696842963601597
1101	22-23	0.2903657672168194
1101	24-25	0.26396014452083705
1101	26-27	0.2591278423124521
1101	28-29	0.20113566247611914
1101	30-31	0.2928287972346908
1101	32-33	0.2641745578848216
1101	34-35	0.3343004948381889
1101	36-37	0.3361398561776383
1101	38-39	0.3404539072496604
1101	40-41	0.32045047140105254
1101	42-43	0.32855617682913874
1101	44-45	0.29670508488737113
1101	46-47	0.2618185225915397
1101	48-49	0.19970880722212314
1101	50-51	0.22227335556917183
1101	52-53	0.21294421786251405
1101	54-55	0.1994178639453139
1101	56-57	0.3050915833163117
1101	58-59	0.29371129550310116
1101	60-61	0.29226874013050264
1101	62-63	0.25102819801090703
1101	64-65	0.2210212598010486
1101	66-67	0.29192405708711533
1101	68-69	0.3425407926334998
1101	70-71	0.25648701409055974
1101	72-73	0.1668355195738087
1101	74-75	0.17939007226206627
1101	76-77	0.18199602896260103
1101	78-79	0.22581001116492416
1101	80-81	0.2279803948393777
1101	82-83	0.18071261240822167
1101	84-85	0.1994958687478956
1101	86-87	0.17394533334103812
1101	88-89	0.2138869714947873
1101	90-91	0.27814782775213587
1101	92-93	0.22525520860860837
1101	94-95	0.21988408908049806
1101	96-97	0.2789914532390938
1101	98-99	0.2818876458095012
1101	100	0.24497455858719874
1102	1	0.05149192495112942
1102	2	0.10755987711672788
1102	3	0.09858335976059607
1102	4	-0.014013681197624805
1102	5	0.10062602520296338
1102	6	0.19100718573768916
1102	7	0.2297874468111445
1102	8	0.2686503290600797
1102	9	0.3327437069463812
1102	10-11	0.2578925258217737
1102	12-13	0.24908385434369507
1102	14-15	0.33503830467991236
1102	16-17	0.3395669785860491
1102	18-19	0.2858961089509151
1102	20-21	0.33540747215128874
1102	22-23	0.23305045620144682
1102	24-25	0.29442932089950347
1102	26-27	0.18707542473600824
1102	28-29	0.20282692883143483
1102	30-31	0.27546708615164306
1102	32-33	0.2362770206731355
1102	34-35	0.27212903611592765
1102	36-37	0.33054501294904526
1102	38-39	0.37521423398346343
1102	40-41	0.2638533142107562
1102	42-43	0.3432915212348462
1102	44-45	0.2788232358930074
1102	46-47	0.26574873318420345
1102	48-49	0.26101706156054405
1102	50-51	0.3046378055451058
1102	52-53	0.3158822846471949
1102	54-55	0.329539946067797
1102	56-57	0.2771389158158897
1102	58-59	0.28674096415748807
1102	60-61	0.28852249855611944
1102	62-63	0.24344579459212667
1102	64-65	0.24620261075995842
1102	66-67	0.3126084882935771
1102	68-69	0.2728803750731714
1102	70-71	0.30073206646181205
1102	72-73	0.28089097409912256
1102	74-75	0.26429625511136123
1102	76-77	0.2757695789101362
1102	78-79	0.24730402688535946
1102	80-81	0.26460674339169543
1102	82-83	0.13674391634275906
1102	84-85	0.1836984984878356
1102	86-87	0.17120070086623684
1102	88-89	0.1403325933946462
1102	90-91	0.23644279644618393
1102	92-93	0.2333109773010449
1102	94-95	0.21811322365076435
1102	96-97	0.1706525313559446
1102	98-99	0.16346254949512584
1102	100	0.02360132602901288
1103	1	-0.015413104153296331
1103	2	0.045376890098193456
1103	3	-0.00641380551912718
1103	4	-0.07447234282133053
1103	5	-0.06417072923648703
1103	6	0.007495258555053397
1103	7	3.478088787076672E-4
1103	8	-0.06662689105012731
1103	9	0.03191163888202908
1103	10-11	0.022989061638270414
1103	12-13	0.025300211456311672
1103	14-15	0.08923548665730152
1103	16-17	0.12079831505957372
1103	18-19	0.1265671610204535
1103	20-21	0.1380491499173715
1103	22-23	0.1570868940065182
1103	24-25	0.17297077965432095
1103	26-27	0.10780475360627406
1103	28-29	0.2001276829446894
1103	30-31	0.20801108256358702
1103	32-33	0.12192755985247317
1103	34-35	0.09299820795045122
1103	36-37	0.11696510101953095
1103	38-39	0.1820119320352731
1103	40-41	0.1867217404750221
1103	42-43	0.13651236536018274
1103	44-45	0.06758633676633252
1103	46-47	0.13758253508773777
1103	48-49	0.11695725672910129
1103	50-51	0.08128238016232103
1103	52-53	0.10665541577048288
1103	54-55	0.19580061024664275
1103	56-57	0.2229245993135507
1103	58-59	0.17395380311042175
1103	60-61	0.13067110002940296
1103	62-63	0.07313099502238174
1103	64-65	0.08535669916440014
1103	66-67	0.10541543413489052
1103	68-69	0.12499248437687527
1103	70-71	0.04998363235353054
1103	72-73	0.037215555395405886
1103	74-75	0.025695810875745906
1103	76-77	0.054752506516045685
1103	78-79	0.11809975810727735
1103	80-81	0.19932599929703798
1103	82-83	0.09609194038740299
1103	84-85	0.08737800423688924
1103	86-87	0.05138222004330828
1103	88-89	0.05598631678032007
1103	90-91	0.09778172608687186
1103	92-93	0.1528470054307931
1103	94-95	0.10045854694478251
1103	96-97	0.18219668697820524
1103	98-99	0.0831983671565979
1103	100	0.020528663993683693
1104	1	-0.024564174643195713
1104	2	0.0399942342643449
1104	3	0.011816671294550218
1104	4	0.0478686598383149
1104	5	-0.03894013402520358
1104	6	0.00829943787849885
1104	7	0.03468530891138499
1104	8	0.032915942925427544
1104	9	0.12290939642389986
1104	10-11	0.10721527912608764
1104	12-13	0.18600659043547552
1104	14-15	0.20302139857471246
1104	16-17	0.1809841227347988
1104	18-19	0.23859053128860808
1104	20-21	0.21932002589905863
1104	22-23	0.22195045757276688
1104	24-25	0.1505002119172616
1104	26-27	0.07921142828065797
1104	28-29	0.05694405628782562
1104	30-31	0.14983782410086377
1104	32-33	0.11621667280042658
1104	34-35	0.1261113478811069
1104	36-37	0.2074457794266067
1104	38-39	0.2168148523103568
1104	40-41	0.25772809024694965
1104	42-43	0.19097419034910246
1104	44-45	0.16710543052790428
1104	46-47	0.17921491033420267
1104	48-49	0.16781775895157836
1104	50-51	0.1434591210833318
1104	52-53	0.04612050789838662
1104	54-55	0.09202363790872425
1104	56-57	0.16734957538191964
1104	58-59	0.16230113966850013
1104	60-61	0.15842833563578296
1104	62-63	0.1303522639011021
1104	64-65	0.11447391989077715
1104	66-67	0.0889726164436695
1104	68-69	0.18617419052745987
1104	70-71	0.12242048758612611
1104	72-73	0.1447559002200336
1104	74-75	0.19841424792276996
1104	76-77	0.1928812650331082
1104	78-79	0.26033447650585373
1104	80-81	0.15910216339387517
1104	82-83	0.1875065872917574
1104	84-85	0.18030070329893277
1104	86-87	0.1306740212145172
1104	88-89	0.08121371394900478
1104	90-91	0.11860730941918263
1104	92-93	0.15024923776723398
1104	94-95	0.2539125823423056
1104	96-97	0.2001891313808173
1104	98-99	0.14718720771535843
1104	100	0.1743413937158529
1105	1	0.02722182461612732
1105	2	0.06817061150000825
1105	3	0.06657582123286332
1105	4	0.034533575959557083
1105	5	-0.010131497860065508
1105	6	0.13649343956320337
1105	7	0.0684673980022481
1105	8	0.1506623351689811
1105	9	0.18390598828118954
1105	10-11	0.173829350100128
1105	12-13	0.24217814230060242
1105	14-15	0.23183704221020207
1105	16-17	0.18286675236605987
1105	18-19	0.21904874580896205
1105	20-21	0.19118311526532494
1105	22-23	0.17098287796492428
1105	24-25	0.1251059326309587
1105	26-27	0.056212778342541014
1105	28-29	0.06309258266308859
1105	30-31	0.13156716730124174
1105	32-33	0.06764677189530488
1105	34-35	-0.014339050004380738
1105	36-37	0.09090705198057947
1105	38-39	0.09954873621931881
1105	40-41	0.15597486082779
1105	42-43	0.09992546687882253
1105	44-45	0.06421804864178426
1105	46-47	0.01739523158573064
1105	48-49	0.054158110200361875
1105	50-51	0.032186904262154314
1105	52-53	0.1202939866856525
1105	54-55	0.0746903294320802
1105	56-57	0.10597153914748247
1105	58-59	0.03857175600990104
1105	60-61	0.00912789933481406
1105	62-63	0.039200676054598205
1105	64-65	0.020747020578738784
1105	66-67	-0.008279767649788994
1105	68-69	0.06325965667694788
1105	70-71	0.08240254317334461
1105	72-73	0.05519970182822931
1105	74-75	0.02269095468142268
1105	76-77	0.031809336258625365
1105	78-79	0.05099324086141621
1105	80-81	0.09673719767825872
1105	82-83	0.10350387526850824
1105	84-85	0.031059830496026564
1105	86-87	-0.038893077099103834
1105	88-89	-0.04204392151630998
1105	90-91	-0.03336693958162584
1105	92-93	-0.054335908749060025
1105	94-95	0.027154279117958424
1105	96-97	0.026279186365663065
1105	98-99	0.004327033324543095
1105	100	0.04130298233318186
1106	1	-0.19133825647829283
1106	2	-0.08736611134249017
1106	3	-5.930089839090158E-4
1106	4	-0.08707140093245158
1106	5	-0.1258834192075824
1106	6	-0.08170742964865951
1106	7	0.006982760882721095
1106	8	0.023218838047917245
1106	9	-0.028830866263909627
1106	10-11	-0.018004393697879095
1106	12-13	0.027774655847210283
1106	14-15	0.03756819069603523
1106	16-17	0.03231918175444548
1106	18-19	0.06155683344806562
1106	20-21	-0.017724315630310628
1106	22-23	-0.004445108078634519
1106	24-25	0.042677296290008826
1106	26-27	-0.058892195073639186
1106	28-29	-0.024959324844274988
1106	30-31	0.03030411158611912
1106	32-33	-0.054348636208949586
1106	34-35	-0.026051923174904346
1106	36-37	0.014593486044283566
1106	38-39	0.029518407967657367
1106	40-41	0.018471661478464796
1106	42-43	-0.04654305183981933
1106	44-45	-0.012163833277099911
1106	46-47	-0.019375387865174787
1106	48-49	-0.06812895216814496
1106	50-51	-0.027762613955339077
1106	52-53	-0.0051715067914699375
1106	54-55	-0.06761780282412388
1106	56-57	-0.08995357996310815
1106	58-59	-0.05967768643436244
1106	60-61	-0.04001189437746433
1106	62-63	-0.029708663391154744
1106	64-65	-0.07857481216022677
1106	66-67	-0.0734555533309873
1106	68-69	0.027444702618026895
1106	70-71	0.011767798587271727
1106	72-73	-0.00407221790143808
1106	74-75	-0.06691256615116004
1106	76-77	-0.0799454365386012
1106	78-79	-0.09928289853351657
1106	80-81	-0.07957042773781353
1106	82-83	-0.06895505140587943
1106	84-85	-0.08460740683977264
1106	86-87	-0.07639033592820965
1106	88-89	-0.053925720331108096
1106	90-91	-0.07603914989120852
1106	92-93	-0.06608960719687218
1106	94-95	-0.11898612065377634
1106	96-97	-0.16056363568478815
1106	98-99	-0.15796519856050395
1106	100	-0.12434829775413192
1107	1	-0.09169375649838685
1107	2	-0.06132655694148781
1107	3	-0.08216290876432453
1107	4	-0.05860947743254741
1107	5	-0.08277146044996897
1107	6	-0.07637712153646703
1107	7	-0.03356104819221173
1107	8	0.010490622014977191
1107	9	-0.02153489514234508
1107	10-11	-0.057725583764337784
1107	12-13	-0.002034550616826891
1107	14-15	-0.0049367143273713054
1107	16-17	-0.00398913504612608
1107	18-19	0.035878624905102185
1107	20-21	7.152840759374612E-4
1107	22-23	-0.035851872443871
1107	24-25	0.007975306147557149
1107	26-27	0.0060563432842002385
1107	28-29	-0.013195124025124016
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2216	40-41	-0.6049614979397973
2216	42-43	-0.7179401987766596
2216	44-45	-0.5845860383801593
2216	46-47	-0.546097468076205
2216	48-49	-0.5851593036555656
2216	50-51	-0.6415769956036002
2216	52-53	-0.617169020622967
2216	54-55	-0.6643785373137732
2216	56-57	-0.777693150894784
2216	58-59	-0.7183659018400448
2216	60-61	-0.6025807114437072
2216	62-63	-0.5553347284750814
2216	64-65	-0.6223237908468775
2216	66-67	-0.6543403122232263
2216	68-69	-0.6430658470625445
2216	70-71	-0.6048974576942925
2216	72-73	-0.6462481794922752
2216	74-75	-0.7555305151983234
2216	76-77	-0.5659772742801081
2216	78-79	-0.630672818462557
2216	80-81	-0.5421459562917974
2216	82-83	-0.7280983834941779
2216	84-85	-0.5895883520506899
2216	86-87	-0.5544047179655394
2216	88-89	-0.6598899471288107
2216	90-91	-0.6374820905733287
2216	92-93	-0.8082999043698784
2216	94-95	-0.732690686799863
2216	96-97	-0.6988511655460456
2216	98-99	-0.6916664965038706
2216	100	-0.6657792842066534
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33640.0
3	7196.0
4	3961.0
5	3213.0
6	2891.0
7	3134.0
8	3584.0
9	4063.0
10	4565.0
11	4942.0
12	5692.0
13	6447.0
14	7001.0
15	7243.0
16	7364.0
17	7264.0
18	7121.0
19	6746.0
20	6938.0
21	7278.0
22	8513.0
23	11801.0
24	18685.0
25	25663.0
26	26475.0
27	22358.0
28	24194.0
29	30107.0
30	36581.0
31	44422.0
32	57111.0
33	77036.0
34	110597.0
35	171906.0
36	313639.0
37	661043.0
38	1081392.0
39	468479.0
40	981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.834790606527825	19.802965490124695	13.539619417853677	23.8226244854938
2	15.277393624609026	20.148746109041436	40.66240519425136	23.911455072098168
3	17.96398930239711	25.69623325195594	30.009755675592547	26.3300217700544
4	12.054390067461853	16.88499039864259	39.43625371616994	31.624365817725618
5	12.794551894133779	38.049789140083334	35.846906927189934	13.308752038592953
6	30.580388395837577	38.46767704628313	18.095734279895705	12.856200277983593
7	26.9688838945009	32.71346501691615	22.967327488296906	17.350323600286043
8	24.722006195135965	36.93245811311592	20.525805253484915	17.8197304382632
9	24.74858290544049	17.23170157118406	21.11023168714783	36.90948383622762
10-11	23.58181941387874	27.25316455506011	29.112358964750594	20.05265706631055
12-13	24.33882190166256	24.550026250284365	28.95526572890894	22.155886119144135
14-15	21.686785546860914	26.42698303943841	25.951973122738693	25.93425829096198
16-17	21.495911932302946	29.75760594488198	26.497846614546404	22.248635508268663
18-19	21.49704130010014	28.775390468005703	28.26014022366975	21.467428008224413
20-21	22.684278977977552	28.108426768033006	27.70162131757912	21.50567293641032
22-23	22.256198384342632	27.909902847299506	27.706466384024246	22.12743238433362
24-25	22.14910621422515	27.930325063232765	27.803168964183715	22.117399758358374
26-27	21.891184580873393	27.897529620762217	27.852117684881467	22.359168113482923
28-29	21.924753145023196	27.95430319857484	27.871801676734915	22.249141979667048
30-31	22.20518847377013	28.412367522800285	27.47363374016053	21.90881026326905
32-33	21.870885352689204	28.01428378738462	27.98253068890405	22.13230017102213
34-35	21.952732854681862	27.804506029432186	28.03832714689446	22.20443396899149
36-37	22.339354662957568	28.0823266289119	27.476524413273644	22.101794294856884
38-39	22.139632081653083	28.233961291454506	27.657055373414618	21.969351253477793
40-41	21.886333684399172	28.00360710009252	27.933303799938443	22.17675541556987
42-43	21.87039319697522	27.884025803955193	27.89840105724956	22.34717994182003
44-45	22.02575783679954	28.006889705382033	27.841872038758275	22.125480419060157
46-47	22.023745144977696	28.196112857637285	27.76054284917705	22.01959914820797
48-49	21.989157984026132	28.006448309203996	27.743712154975725	22.260681551794143
50-51	21.990106575193618	27.979153902342198	27.7898050529977	22.240934469466485
52-53	22.085076404734043	27.863731747507625	27.792306502175805	22.258885345582524
54-55	22.132640147408647	28.33715883287869	27.5557776458053	21.97442337390736
56-57	21.935170387637886	28.036937348297325	27.700052269832497	22.327839994232292
58-59	21.82259649814237	27.62857106810022	28.06831590254781	22.480516531209595
60-61	22.02379584576721	28.14972648099244	27.690564792006754	22.13591288123359
62-63	21.956369219483335	28.05473816830389	27.65892764366719	22.32996496854559
64-65	21.917405162106025	27.87135332596678	27.78177713220704	22.429464379720155
66-67	21.797723039553997	27.9639063964658	27.67769049481136	22.560680069168843
68-69	22.223146769393537	28.021689676376116	27.68913806937128	22.066025484859072
70-71	22.15052910779994	27.806886053303813	27.748193917734593	22.294390921161654
72-73	21.95691802524329	27.950464379546215	27.861255928832684	22.231361666377815
74-75	21.98631025757477	28.103485874117805	27.640417781045823	22.269786087261593
76-77	22.02508647570053	28.00184777843839	27.611117126536328	22.361948619324753
78-79	22.139647645430692	28.04178265576478	27.678621709374834	22.139947989429693
80-81	22.131935774606266	27.804455101358645	27.82224943846478	22.24135968557031
82-83	21.948242785113326	27.90172661708446	27.94360993996824	22.206420657833974
84-85	21.986562983742573	27.866905577654606	27.689133819534113	22.45739761906871
86-87	22.112355825379897	27.975784880220232	27.666241328951624	22.245617965448243
88-89	21.922925321094443	27.866560454799018	27.713068789702756	22.497445434403783
90-91	21.870423162891182	27.825022440297097	27.828445918208306	22.47610847860341
92-93	22.103166660385128	27.934199724943763	27.70055658420936	22.26207703046175
94-95	21.884393554839853	27.724837566906846	27.818503776153676	22.572265102099625
96-97	21.96024185775248	27.66381309916171	27.852908057625537	22.523036985460276
98-99	21.88928577649455	27.865248882965272	27.706395402964386	22.539069937575793
100	22.05037286032177	27.656988326584138	27.667260043531655	22.62537876956244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	466.0
1	449.0
2	419.0
3	385.0
4	408.0
5	430.5
6	398.5
7	378.0
8	340.5
9	299.5
10	275.5
11	271.5
12	279.0
13	300.0
14	341.5
15	410.5
16	512.5
17	673.0
18	931.0
19	1265.0
20	1816.5
21	2741.0
22	4103.5
23	6377.0
24	9490.5
25	13284.5
26	18602.0
27	25506.0
28	34111.5
29	43333.5
30	52220.0
31	61351.5
32	70798.5
33	81598.5
34	91373.0
35	97820.5
36	104014.0
37	110679.0
38	114871.0
39	116563.0
40	118557.0
41	120712.5
42	121035.0
43	120363.0
44	118448.5
45	117180.0
46	115436.5
47	111949.0
48	109884.0
49	107355.5
50	103898.0
51	100239.0
52	94013.0
53	89930.5
54	88013.5
55	83643.5
56	76972.0
57	70644.0
58	63935.5
59	56333.5
60	49813.0
61	42713.5
62	36362.0
63	30473.0
64	24665.0
65	20178.0
66	16340.0
67	12534.0
68	9832.0
69	7814.0
70	6007.5
71	4504.0
72	3452.0
73	2672.5
74	1976.5
75	1330.5
76	790.5
77	492.0
78	350.5
79	238.0
80	163.0
81	105.5
82	64.5
83	37.0
84	22.5
85	20.5
86	15.5
87	12.0
88	12.0
89	13.0
90	7.5
91	5.5
92	5.0
93	3.0
94	6.5
95	5.0
96	2.5
97	2.0
98	0.0
99	0.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06312915270050486
2	0.04295664170918804
3	0.05769578292456982
4	0.07714784709476817
5	0.08975566646434117
6	0.09041607605036643
7	0.09263745374881502
8	0.10488504970782879
9	0.099961996430186
10-11	0.10529030104470792
12-13	0.11055856842413665
14-15	0.10644601782025212
16-17	0.07666754921402254
18-19	0.1011177132057302
20-21	0.09424344978755825
22-23	0.059166695184353336
24-25	0.021433292928274117
26-27	0.1845844792940582
28-29	0.06713663814297627
30-31	0.0774480332702342
32-33	0.0737557433120021
34-35	0.021868562882699852
36-37	0.053928446422471216
38-39	0.07945928064585656
40-41	0.0849676969656581
42-43	0.07891894553001771
44-45	0.07599213031922399
46-47	0.08267127272334303
48-49	0.07929417824935024
50-51	0.07473134838226668
52-53	0.07837861041417887
54-55	0.06871261556417291
56-57	0.07162442146619333
58-59	0.0687426341817195
60-61	0.06470513012170148
62-63	0.07205969142061906
64-65	0.06773701049390832
66-67	0.06935801584142484
68-69	0.06931298791510496
70-71	0.06092278431082958
72-73	0.05958695583000577
74-75	0.06177831491090774
76-77	0.05590967518054698
78-79	0.0525475900153275
80-81	0.046678950284966735
82-83	0.053388111306632374
84-85	0.043061706870601145
86-87	0.040915375716019074
88-89	0.04333187442852057
90-91	0.039594556543968575
92-93	0.04207109249156327
94-95	0.04072025470196616
96-97	0.043752135074173
98-99	0.044067330558412325
100	0.05208230144335517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3331266.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.94617749925828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17951903603014	48.58155704549337
2	13.549122782676507	17.05730975275199
3	4.254269018923513	8.033699183842645
4	1.7825155808099287	4.48810168579421
5	0.9526586045249615	2.998310880831196
6	0.5735084380034778	2.1660098361533566
7	0.3619978725427618	1.595046763760139
8	0.24644555217397984	1.2410244376836843
9	0.1791179757814561	1.0147312707162643
>10	0.8638139960775137	9.472565955840013
>50	0.04263187409538267	1.834544240232225
>100	0.014065603014793473	1.3914412496115005
>500	3.3366534530648856E-4	0.12565769728944262
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0018617546602403E-5	0.0
5	0.0	0.0	0.0	3.0018617546602403E-5	0.0
6	0.0	0.0	0.0	3.0018617546602403E-5	0.0
7	0.0	0.0	0.0	3.0018617546602403E-5	0.0
8	0.0	0.0	0.0	3.0018617546602403E-5	0.0
9	0.0	0.0	0.0	9.00558526398072E-5	0.0
10-11	0.0	0.0	0.0	1.801117052796144E-4	0.0
12-13	0.0	0.0	0.0	2.1013032282621682E-4	0.0
14-15	0.0	0.0	0.0	2.7016755791942163E-4	0.0
16-17	0.0	0.0	0.0	3.00186175466024E-4	0.0
18-19	0.0	0.0	0.0	3.00186175466024E-4	0.0
20-21	0.0	0.0	0.0	3.3020479301262644E-4	0.0
22-23	0.0	0.0	0.0	3.602234105592288E-4	0.0
24-25	0.0	0.0	0.0	3.902420281058312E-4	0.0
26-27	0.0	0.0	0.0	5.403351158388433E-4	0.0
28-29	0.0	0.0	0.0	9.455864527179757E-4	0.0
30-31	0.0	0.0	0.0	0.0033320665476728665	0.0
32-33	0.0	0.0	0.0	0.00837519429550207	0.0
34-35	0.0	0.0	0.0	0.015309494948767226	0.0
36-37	0.0	0.0	0.0	0.0287128076833252	0.0
38-39	0.0	0.0	0.0	0.05254759001532751	0.0
40-41	0.0	0.0	0.0	0.08315157060408865	0.0
42-43	0.0	0.0	0.0	0.11462609110170127	0.0
44-45	0.0	0.0	0.0	0.1498979667189591	0.0
46-47	0.0	0.0	0.0	0.19271952464918743	0.0
48-49	0.0	0.0	0.0	0.24511702157678195	0.0
50-51	0.0	0.0	0.0	0.29939068210103903	0.0
52-53	0.0	0.0	0.0	0.357641809450221	0.0
54-55	0.0	0.0	0.0	0.4206658969893128	0.0
56-57	0.0	0.0	0.0	0.4961477108102445	0.0
58-59	0.0	0.0	0.0	0.5827364131234192	0.0
60-61	0.0	0.0	0.0	0.6747584852125288	0.0
62-63	0.0	0.0	0.0	0.7710281916844828	0.0
64-65	0.0	0.0	0.0	0.8769038557713494	0.0
66-67	0.0	0.0	0.0	0.9956575067857085	0.0
68-69	0.0	0.0	0.0	1.1322872445490693	0.0
70-71	0.0	0.0	0.0	1.2812246155065372	0.0
72-73	0.0	0.0	0.0	1.44186924730718	0.0
74-75	0.0	0.0	0.0	1.6037596517360067	0.0
76-77	0.0	0.0	0.0	1.7777175404185677	0.0
78-79	0.0	0.0	0.0	1.980148087844081	0.0
80-81	0.0	0.0	0.0	2.2060531941910373	0.0
82-83	0.0	0.0	0.0	2.445766864609431	0.0
84-85	0.0	0.0	0.0	2.68123290064498	0.0
86-87	0.0	0.0	0.0	2.931378040660818	0.0
88	0.0	0.0	0.0	3.1378160735288025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3690	0.0	34.019787	1
TCAACGC	3720	0.0	32.35274	4
ATCAACG	3870	0.0	31.4632	3
GGTATCA	2645	0.0	31.462578	1
CAACGCA	4150	0.0	29.113813	5
AACGCAG	4205	0.0	28.725246	6
GTACATG	9505	0.0	26.710888	1
TATCAAC	4645	0.0	26.51732	2
TACATGG	9500	0.0	25.584738	2
ACATGGG	9300	0.0	24.618416	3
ACGCAGA	5215	0.0	22.531435	7
CGCAGAG	5380	0.0	21.686543	8
ACACCGT	660	0.0	20.651457	6
CATGGGG	6975	0.0	20.355267	4
TACACCG	555	0.0	19.48269	5
GAGTACA	6505	0.0	19.297928	1
TCTATAC	1180	0.0	17.928526	3
GTATATA	2475	0.0	17.66666	1
ACCGTTC	460	0.0	17.386496	8
AGTACAT	6670	0.0	16.845587	2
>>END_MODULE