FastQCFastQC Report
Wed 25 May 2016
SRR1033101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331266
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60770.1824231388307028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50300.15099364625941009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50300.052.291741
GTATCAA87100.042.8621441
TCAACGC105300.034.9027144
ATCAACG105750.034.7557563
CAACGCA107650.034.183425
TATCAAC110950.033.5462842
AACGCAG111000.033.1517566
ACGCAGA128200.028.5206437
CGCAGAG129050.028.1511258
GTACATG91350.025.8005141
GCAGAGT143300.024.958179
TACATGG94100.024.1324882
ACATGGG91550.023.4102943
TACACCG5250.022.3794025
GAGTACT98100.021.67839412-13
AGAGTAC131550.021.4176910-11
GTGGTAT20800.020.8492931
CAGAGTA142700.020.74869710-11
TAGACCG2109.545329E-720.141465
AGTACTT103750.019.68245312-13
Produced by FastQC (version 0.11.5)