##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033101_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3331266
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73973138140275	34.0	31.0	34.0	31.0	34.0
2	32.73644494315374	34.0	31.0	34.0	31.0	34.0
3	32.83779500045929	34.0	31.0	34.0	31.0	34.0
4	36.316606659450194	37.0	37.0	37.0	35.0	37.0
5	36.30260537585411	37.0	37.0	37.0	35.0	37.0
6	36.37104962497741	37.0	37.0	37.0	35.0	37.0
7	36.3211349679071	37.0	37.0	37.0	35.0	37.0
8	36.33381423158643	37.0	37.0	37.0	35.0	37.0
9	38.144715552585716	39.0	39.0	39.0	37.0	39.0
10-11	38.10473060392055	39.0	39.0	39.0	36.0	39.0
12-13	38.07689028735622	39.0	39.0	39.0	35.0	39.0
14-15	39.57671212686108	41.0	40.0	41.0	37.0	41.0
16-17	39.57815121938626	41.0	40.0	41.0	37.0	41.0
18-19	39.59600764394077	41.0	40.0	41.0	37.0	41.0
20-21	39.5863583094235	41.0	40.0	41.0	37.0	41.0
22-23	39.519257993807756	41.0	39.5	41.0	37.0	41.0
24-25	39.4633766862208	41.0	39.0	41.0	36.0	41.0
26-27	39.355211802359825	41.0	39.0	41.0	36.0	41.0
28-29	39.30135074773375	41.0	39.0	41.0	36.0	41.0
30-31	39.29485757066533	41.0	39.0	41.0	36.0	41.0
32-33	39.250571554478086	41.0	39.0	41.0	35.5	41.0
34-35	39.168156490655505	41.0	39.0	41.0	35.0	41.0
36-37	39.080332822416466	41.0	39.0	41.0	35.0	41.0
38-39	38.983618089939384	41.0	39.0	41.0	35.0	41.0
40-41	38.87755420912049	41.0	39.0	41.0	35.0	41.0
42-43	38.7575432283102	41.0	39.0	41.0	35.0	41.0
44-45	38.58437648029307	41.0	38.5	41.0	35.0	41.0
46-47	38.37320210994859	41.0	38.0	41.0	34.5	41.0
48-49	38.16095727570239	40.0	38.0	41.0	34.0	41.0
50-51	38.01815210793734	40.0	38.0	41.0	34.0	41.0
52-53	37.88276138861322	40.0	38.0	41.0	34.0	41.0
54-55	37.73036722375217	40.0	37.0	41.0	33.0	41.0
56-57	37.57861605767897	40.0	37.0	41.0	33.0	41.0
58-59	37.3983716701098	40.0	37.0	41.0	33.0	41.0
60-61	37.204918490447774	40.0	36.0	41.0	33.0	41.0
62-63	36.96837553650774	39.0	36.0	41.0	32.5	41.0
64-65	36.703357822521525	39.0	35.0	41.0	32.0	41.0
66-67	36.42301815586026	39.0	35.0	41.0	32.0	41.0
68-69	36.117438685472735	38.0	35.0	40.5	31.5	41.0
70-71	35.794349505563346	37.0	35.0	40.0	31.0	41.0
72-73	35.448465388233785	37.0	35.0	39.0	31.0	41.0
74-75	35.10398434108835	36.0	35.0	39.0	31.0	41.0
76-77	34.74896420760156	36.0	35.0	39.0	31.0	40.5
78-79	34.39070506528149	35.0	35.0	37.5	30.0	39.5
80-81	34.06377260176761	35.0	34.0	37.0	30.0	39.0
82-83	33.76696682282351	35.0	34.0	36.5	30.0	39.0
84-85	33.540682281150765	35.0	34.0	36.0	30.0	37.5
86-87	33.332496114089956	35.0	34.0	36.0	30.0	37.0
88-89	33.12518258824123	35.0	34.0	35.5	30.0	37.0
90-91	32.942636373078585	35.0	34.0	35.0	30.0	36.0
92-93	32.771746086923116	35.0	34.0	35.0	29.0	36.0
94-95	32.618999353398976	35.0	34.0	35.0	29.0	36.0
96-97	32.477801382417375	35.0	34.0	35.0	29.0	36.0
98-99	32.35827310097723	35.0	34.0	35.0	29.0	35.5
100	32.25786352695942	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13707982903316918
1101	2	0.04034281802598372
1101	3	0.06878641218206383
1101	4	0.08032554587557428
1101	5	0.10260956556955136
1101	6	0.1335517522072962
1101	7	0.14948164715656986
1101	8	0.1555102226998244
1101	9	0.16296800978596337
1101	10-11	0.22844947643911695
1101	12-13	0.23141073578213422
1101	14-15	0.3307436947871025
1101	16-17	0.32300879866328813
1101	18-19	0.2982661190931921
1101	20-21	0.28616152017484353
1101	22-23	0.2797714613778979
1101	24-25	0.3210918769060669
1101	26-27	0.35924641937430124
1101	28-29	0.31699721391271396
1101	30-31	0.3266716717896756
1101	32-33	0.29300417795567313
1101	34-35	0.26392739344704097
1101	36-37	0.2426195066551884
1101	38-39	0.20341410010244232
1101	40-41	0.2190648597446554
1101	42-43	0.23194788726842575
1101	44-45	0.18175665849620515
1101	46-47	0.1587832485373326
1101	48-49	0.127018161059965
1101	50-51	0.20074347673106274
1101	52-53	0.1569300639684812
1101	54-55	0.12958308481628222
1101	56-57	0.14328728680563074
1101	58-59	0.0744339396982241
1101	60-61	0.08748998034329958
1101	62-63	0.06664034411507913
1101	64-65	0.09655436475920709
1101	66-67	0.14321046773956425
1101	68-69	0.10386502819228127
1101	70-71	0.05943366080519752
1101	72-73	0.06934181781874571
1101	74-75	0.07515893977458177
1101	76-77	0.0383328846502522
1101	78-79	0.08117164578497693
1101	80-81	0.04395805589887658
1101	82-83	0.029820012464035983
1101	84-85	0.07322330747915373
1101	86-87	0.09969020773403514
1101	88-89	0.09176632552198782
1101	90-91	0.07711704089028615
1101	92-93	0.13109816337345137
1101	94-95	0.14176474173507359
1101	96-97	0.1396667273925729
1101	98-99	0.1211404562273799
1101	100	0.09933086223466603
1102	1	0.18375499432333697
1102	2	0.10967164829719422
1102	3	0.12654613877610643
1102	4	0.10362063540313926
1102	5	0.08596424615623732
1102	6	0.14401623621800752
1102	7	0.15723100220771613
1102	8	0.19314553105687082
1102	9	0.23317409133649392
1102	10-11	0.22242673154483583
1102	12-13	0.23274312661409624
1102	14-15	0.31405205586180074
1102	16-17	0.34211554701339963
1102	18-19	0.23994366077571527
1102	20-21	0.2627556162495637
1102	22-23	0.262000426781249
1102	24-25	0.30327643599466114
1102	26-27	0.30594157254084564
1102	28-29	0.35688974257543293
1102	30-31	0.3503100291789423
1102	32-33	0.3407755549139324
1102	34-35	0.3378426106150698
1102	36-37	0.37520971390479474
1102	38-39	0.3061381405333421
1102	40-41	0.3201396738120934
1102	42-43	0.3482603103629316
1102	44-45	0.3424317452445962
1102	46-47	0.34065414397404936
1102	48-49	0.3424558502399293
1102	50-51	0.3989271980406528
1102	52-53	0.39221933939034415
1102	54-55	0.3339680825305109
1102	56-57	0.3521940758363655
1102	58-59	0.39435127950823556
1102	60-61	0.4095101756505315
1102	62-63	0.38392633286547806
1102	64-65	0.4473392287510194
1102	66-67	0.42867661321102446
1102	68-69	0.4308294286264811
1102	70-71	0.3757078703023069
1102	72-73	0.3487088918957326
1102	74-75	0.4096395369337884
1102	76-77	0.37486543619077395
1102	78-79	0.35166828157868224
1102	80-81	0.35354277126548084
1102	82-83	0.34491977059562373
1102	84-85	0.34146252157800916
1102	86-87	0.34096315854876025
1102	88-89	0.3871316052213558
1102	90-91	0.32979677570101273
1102	92-93	0.36798259216323714
1102	94-95	0.41080684415759094
1102	96-97	0.42814520190979266
1102	98-99	0.40836508169384444
1102	100	0.4068511370906194
1103	1	0.14272785478908645
1103	2	0.1152287679107431
1103	3	0.10318775246975065
1103	4	0.1106595759167277
1103	5	0.14284941280283903
1103	6	0.09944647297539433
1103	7	0.12803163594243472
1103	8	0.15060443732082973
1103	9	0.17453168935762875
1103	10-11	0.17142302699258494
1103	12-13	0.21732069504540874
1103	14-15	0.23642926005096143
1103	16-17	0.2849489470079689
1103	18-19	0.21056285299692945
1103	20-21	0.2551687592553762
1103	22-23	0.23268184693727534
1103	24-25	0.23087855388018852
1103	26-27	0.19192907453097519
1103	28-29	0.24878088931686193
1103	30-31	0.21757366981407245
1103	32-33	0.1501722157687766
1103	34-35	0.15560752104956066
1103	36-37	0.14350098917942944
1103	38-39	0.14279306382114498
1103	40-41	0.16983183483974074
1103	42-43	0.18482417311587085
1103	44-45	0.16286750582334975
1103	46-47	0.15133807848813774
1103	48-49	0.11312605771814788
1103	50-51	0.1520693689704018
1103	52-53	0.10051909940991521
1103	54-55	0.10735156387766409
1103	56-57	0.09112337674774551
1103	58-59	0.07627542355862715
1103	60-61	0.10140345814011198
1103	62-63	0.1255518216605651
1103	64-65	0.12039642602813672
1103	66-67	0.04664903099443052
1103	68-69	0.07247758547583061
1103	70-71	0.08606076385274264
1103	72-73	0.07135141425313662
1103	74-75	0.05396810625435222
1103	76-77	0.047642801920176225
1103	78-79	0.07337981190048026
1103	80-81	0.08454854361026065
1103	82-83	0.050508198335506904
1103	84-85	0.07555381898676217
1103	86-87	0.0765640415835449
1103	88-89	0.05085203967136209
1103	90-91	0.027330666141018867
1103	92-93	0.035990686648737835
1103	94-95	0.10461603835922517
1103	96-97	0.10146252360394925
1103	98-99	0.09009351354988837
1103	100	0.11297850146090838
1104	1	0.11445949260894395
1104	2	0.12094859547602255
1104	3	0.11652452381558476
1104	4	0.05186540698014852
1104	5	0.07030179324834052
1104	6	0.08058661204216122
1104	7	0.11082560655368923
1104	8	0.10278856501992806
1104	9	0.10913746081140374
1104	10-11	0.11970171441506494
1104	12-13	0.10126891159276141
1104	14-15	0.15094292040819823
1104	16-17	0.21573013964161447
1104	18-19	0.14399141796938864
1104	20-21	0.14602768906800634
1104	22-23	0.16713429909362532
1104	24-25	0.214851505069376
1104	26-27	0.17150798522817468
1104	28-29	0.20782633569638165
1104	30-31	0.1597767766120768
1104	32-33	0.2026551060074695
1104	34-35	0.08905590454874357
1104	36-37	0.14749688504033287
1104	38-39	0.10287740717569704
1104	40-41	0.09909220648870587
1104	42-43	0.12306835996148635
1104	44-45	0.09961785662761002
1104	46-47	0.11605127335975851
1104	48-49	0.14903342316973323
1104	50-51	0.2331697215861226
1104	52-53	0.18246165770952416
1104	54-55	0.13764844213989846
1104	56-57	0.1687761694185994
1104	58-59	0.187571577615941
1104	60-61	0.2140389851159057
1104	62-63	0.17979265490332352
1104	64-65	0.19924215861612282
1104	66-67	0.10520584447786518
1104	68-69	0.1521387722381533
1104	70-71	0.14551641405168425
1104	72-73	0.15654806259244225
1104	74-75	0.12976010500388924
1104	76-77	0.09856843216333289
1104	78-79	0.07591214807946045
1104	80-81	0.11809425965245879
1104	82-83	0.1047112153081855
1104	84-85	0.12753704424959977
1104	86-87	0.1338009271812055
1104	88-89	0.1415260245799672
1104	90-91	0.1150257093897551
1104	92-93	0.07921620066055368
1104	94-95	0.15267097017975573
1104	96-97	0.21069467964676392
1104	98-99	0.2094576987969532
1104	100	0.24438569809318267
1105	1	-0.012080349421523806
1105	2	0.05130478519442505
1105	3	0.09335081933488709
1105	4	0.04349716520602698
1105	5	0.0032388475219917723
1105	6	0.0761208701764815
1105	7	0.060930434343781315
1105	8	0.07334727327606316
1105	9	0.06851687394766515
1105	10-11	0.07924368764202683
1105	12-13	0.10565274538508618
1105	14-15	0.14788197802507597
1105	16-17	0.16058551272529087
1105	18-19	0.17474746981250888
1105	20-21	0.12897877811484193
1105	22-23	0.15811720522908246
1105	24-25	0.1327572709878737
1105	26-27	0.19221691117203932
1105	28-29	0.1336006314224676
1105	30-31	0.15302732659933582
1105	32-33	0.18512241516661732
1105	34-35	0.20074883402437393
1105	36-37	0.18332746926371613
1105	38-39	0.19591929320593948
1105	40-41	0.22380157826634672
1105	42-43	0.23525928259015672
1105	44-45	0.22355719996416923
1105	46-47	0.27949939566840953
1105	48-49	0.2199871015429693
1105	50-51	0.2827564328837582
1105	52-53	0.31919512143470286
1105	54-55	0.2801876611665861
1105	56-57	0.2279829475727979
1105	58-59	0.1899816185541141
1105	60-61	0.20912516179644314
1105	62-63	0.2647409126677047
1105	64-65	0.25961098690229534
1105	66-67	0.2795326017630231
1105	68-69	0.37811494921881916
1105	70-71	0.3777369082052715
1105	72-73	0.33515241777494253
1105	74-75	0.3016924889968706
1105	76-77	0.24880905944034737
1105	78-79	0.27382572005550543
1105	80-81	0.3434883538357738
1105	82-83	0.31836680501524484
1105	84-85	0.28471367891063437
1105	86-87	0.22417666906407163
1105	88-89	0.21814491219824816
1105	90-91	0.2802947162606415
1105	92-93	0.3188439876339828
1105	94-95	0.315082846281868
1105	96-97	0.2967941457965182
1105	98-99	0.29859629162021406
1105	100	0.3082985875680606
1106	1	0.01687134466949658
1106	2	0.03833713511887282
1106	3	0.02376242786977656
1106	4	-1.9397071623217244E-4
1106	5	0.005913345563826056
1106	6	-0.003865580381649636
1106	7	0.019555974915320462
1106	8	0.012033900090685279
1106	9	-1.8655103062314993E-5
1106	10-11	0.04034636195047625
1106	12-13	0.04180453958018404
1106	14-15	0.08408274991352016
1106	16-17	0.09357385350639191
1106	18-19	0.07612048834882756
1106	20-21	0.11953644761084092
1106	22-23	0.0871602822163311
1106	24-25	0.07183300187725195
1106	26-27	0.06300301262980668
1106	28-29	0.053254147420481956
1106	30-31	0.028104758752597547
1106	32-33	0.08911981650500422
1106	34-35	0.013238209024770242
1106	36-37	0.016327311903125974
1106	38-39	0.01998459342654968
1106	40-41	0.048342941666291495
1106	42-43	0.001063120292613462
1106	44-45	0.017829716905715998
1106	46-47	0.041823478853387996
1106	48-49	0.09524679391250146
1106	50-51	0.054590340994025155
1106	52-53	0.12506321787083152
1106	54-55	0.05665681817517054
1106	56-57	-0.02851184214858904
1106	58-59	0.04886689889295326
1106	60-61	8.809204672317605E-4
1106	62-63	-0.01741161587681006
1106	64-65	-0.00930429306368552
1106	66-67	0.018395801061785733
1106	68-69	-0.017023682932155282
1106	70-71	-0.08982924169933426
1106	72-73	-0.04144098458218792
1106	74-75	0.018011350843757157
1106	76-77	-0.07539268315439074
1106	78-79	-0.05272935330675921
1106	80-81	-0.09497360755969453
1106	82-83	-0.04978290620594805
1106	84-85	-0.09586928835393849
1106	86-87	-0.09962769064580357
1106	88-89	-0.025668130782676712
1106	90-91	-0.08716779052512891
1106	92-93	-0.002511676999631618
1106	94-95	-0.02078633082336978
1106	96-97	-0.08623195520623028
1106	98-99	-0.030588789079217804
1106	100	-0.0716438146645686
1107	1	-0.01563297577011724
1107	2	0.016485392876482763
1107	3	0.020505930768941028
1107	4	0.014546309664766
1107	5	0.028945947071321143
1107	6	0.008596650024749408
1107	7	0.025301337521689504
1107	8	0.06480062558109978
1107	9	0.05835094069378499
1107	10-11	0.06085392683015556
1107	12-13	0.07308963744316799
1107	14-15	0.10017780323852321
1107	16-17	0.06158725508988283
1107	18-19	0.0508092888194156
1107	20-21	0.04896092486504244
1107	22-23	0.09159928272418227
1107	24-25	0.058861087474930684
1107	26-27	0.0400279560895882
1107	28-29	0.06503351088180409
1107	30-31	0.07347321095738835
1107	32-33	0.02997363767351402
1107	34-35	0.05585802130029549
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2216	32-33	-0.32525229950063306
2216	34-35	-0.35428553407893304
2216	36-37	-0.3107274246563918
2216	38-39	-0.31894694745321317
2216	40-41	-0.23347048286546368
2216	42-43	-0.2527383539002557
2216	44-45	-0.3084920571058447
2216	46-47	-0.2698845655941682
2216	48-49	-0.30632661225506297
2216	50-51	-0.4046438879824876
2216	52-53	-0.3768189653664038
2216	54-55	-0.37424627572340796
2216	56-57	-0.37957512661451176
2216	58-59	-0.3078388986652314
2216	60-61	-0.3503174691647857
2216	62-63	-0.3867040305339202
2216	64-65	-0.42258832127916435
2216	66-67	-0.36948942860726675
2216	68-69	-0.4244950764315476
2216	70-71	-0.373842429286384
2216	72-73	-0.37298269112870486
2216	74-75	-0.4793532230059583
2216	76-77	-0.4023534102516777
2216	78-79	-0.40858177060955825
2216	80-81	-0.40907648591251444
2216	82-83	-0.4041494098515557
2216	84-85	-0.45862729155989257
2216	86-87	-0.5028341282270219
2216	88-89	-0.5661602384621744
2216	90-91	-0.5571079610163707
2216	92-93	-0.5364505518819414
2216	94-95	-0.432883738827357
2216	96-97	-0.5969528273494724
2216	98-99	-0.6627175562664647
2216	100	-0.6169142584398095
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10.0
3	6.0
4	8.0
5	30.0
6	79.0
7	175.0
8	359.0
9	758.0
10	1349.0
11	2288.0
12	3559.0
13	5577.0
14	6692.0
15	8989.0
16	10823.0
17	16059.0
18	18764.0
19	9915.0
20	6475.0
21	6086.0
22	6439.0
23	6803.0
24	7873.0
25	9540.0
26	11881.0
27	15524.0
28	19932.0
29	25516.0
30	33091.0
31	42455.0
32	54166.0
33	71474.0
34	99038.0
35	152765.0
36	282565.0
37	643039.0
38	1175212.0
39	573866.0
40	2086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77623612512613	19.90780116284657	13.733091874489453	23.58287083753784
2	15.538609317080834	20.386914703264285	40.31037074887047	23.764105230784416
3	18.03804963714005	25.880957920253362	30.32143312795035	25.75955931465624
4	12.198324501146288	17.07277315863685	39.06883642377327	31.660065916443596
5	12.492120098701392	37.82835923944693	35.90749446155509	13.772026200296583
6	30.377757625261047	38.59872366726974	17.698362452919508	13.3251562545497
7	26.683879341967888	32.760277924368694	22.90300444335577	17.65283829030765
8	25.020150063564632	36.13905269935805	20.446528410463923	18.394268826613395
9	25.17760515071447	16.29440579047125	21.346899346974993	37.181089711839284
10-11	23.834528031094663	26.631783833501387	29.052739109408577	20.480949025995372
12-13	24.921801501291103	24.035651911315398	29.23019356604966	21.81235302134384
14-15	21.935277032605836	25.704391987475006	26.841334336157242	25.518996643761916
16-17	21.103231209812503	29.23937188931515	27.247888186451974	22.40950871442037
18-19	21.00442852114711	27.69522528722348	29.99685553234937	21.303490659280044
20-21	22.13210296989282	27.08073359519582	29.676084010128285	21.111079424783078
22-23	21.634362534731643	26.936776999426044	29.693545017254753	21.73531544858756
24-25	21.52938171037535	27.02443727857225	29.778096446289084	21.66808456476332
26-27	21.324622113648005	27.012170621952446	29.7891983497533	21.874008914646247
28-29	21.399586222133635	27.06732835903335	29.803518552762082	21.729566866070936
30-31	21.588046573934054	27.56510482137028	29.391499469045147	21.455349135650522
32-33	21.28791276349592	27.15701778242866	29.893830153461177	21.66123930061424
34-35	21.402043547408102	26.984065517433915	29.83638952878575	21.77750140637223
36-37	21.826251641267913	27.317737460773174	29.233240455730648	21.62277044222827
38-39	21.677974679896472	27.46233714149516	29.31423068587138	21.545457492736997
40-41	21.506414085712862	27.301275012292635	29.41193157428026	21.780379327714243
42-43	21.529502597510977	27.22260846176799	29.252902650223668	21.99498629049737
44-45	21.70815839983958	27.376558941855738	29.1017739201853	21.81350873811938
46-47	21.824720691773038	27.61706810563912	28.748875052307444	21.809336150280405
48-49	21.8389645257989	27.54870070417673	28.49056484831893	22.121769921705443
50-51	21.88330202391523	27.62740951938392	28.371556039055424	22.117732417645424
52-53	21.980322195825853	27.567387293599488	28.311488785344668	22.140801725229988
54-55	21.992569791784867	28.009366409046894	28.07064941676828	21.927414382399967
56-57	21.754657238419266	27.69041859761424	28.302513218698238	22.252410945268256
58-59	21.675468125331328	27.27832301593448	28.69835371897651	22.347855139757677
60-61	21.844593016588888	27.73100376853725	28.31135370156571	22.11304951330815
62-63	21.791324979752442	27.623957378366065	28.33954118344197	22.245176458439524
64-65	21.78053328674444	27.395605754689058	28.41251644269776	22.41134451586874
66-67	21.627603439653274	27.527357467101094	28.34584509312676	22.499194000118873
68-69	22.0634066769971	27.582733009571758	28.281763225784374	22.072097087646767
70-71	21.99971752476649	27.48794714801327	28.24022882595218	22.272106501268063
72-73	21.859127608325508	27.567800253117742	28.351693775560932	22.221378362995818
74-75	21.89535405731432	27.71822326166829	28.097749155987906	22.28867352502948
76-77	21.981868154629502	27.655341843011033	28.008675980843318	22.354114021516143
78-79	22.06901220136729	27.723709244473422	28.067242303676743	22.14003625048255
80-81	22.015969304162443	27.532715790333164	28.14092300044488	22.310391905059518
82-83	21.849775895808886	27.65703278306156	28.266846538384993	22.22634478274456
84-85	21.840540503220097	27.528543202494188	28.12687428743307	22.50404200685265
86-87	21.97113184441209	27.632717883400172	28.078256545556417	22.317893726631322
88-89	21.70660653209936	27.583130440708146	28.08659833984526	22.62366468734723
90-91	21.626425912584054	27.554980187319885	28.16579610951009	22.652797790585975
92-93	21.87272513866429	27.642066169215312	28.037338570211094	22.447870121909304
94-95	21.637698298400736	27.438421360640014	28.160958271350818	22.762922069608436
96-97	21.644567661140677	27.426571669535704	28.14599947737469	22.78286119194893
98-99	21.561033025922306	27.54795329817949	28.05741487760494	22.83359879829326
100	21.73191813562772	27.402404971563364	27.942860161872396	22.92281673093653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	33.0
2	105.5
3	198.5
4	150.5
5	127.5
6	290.0
7	622.5
8	1134.0
9	1394.0
10	1179.5
11	979.5
12	1099.5
13	1323.0
14	1559.0
15	1852.5
16	2145.5
17	2433.5
18	2838.0
19	3365.0
20	4101.5
21	5072.5
22	6418.5
23	8632.0
24	11654.0
25	15099.5
26	19991.5
27	26805.5
28	34975.0
29	43266.5
30	51919.0
31	61385.0
32	70507.5
33	80954.0
34	90394.5
35	96804.0
36	103212.5
37	109881.0
38	113598.5
39	114999.0
40	117630.0
41	119874.5
42	120891.0
43	120374.0
44	117853.5
45	116570.5
46	115150.0
47	111500.5
48	108725.0
49	106250.5
50	103106.5
51	99419.5
52	92794.0
53	88288.0
54	86742.5
55	82832.5
56	76237.0
57	70093.0
58	63075.0
59	55392.0
60	49071.0
61	41963.5
62	35742.0
63	30172.0
64	24415.0
65	19635.5
66	15651.0
67	11964.0
68	9424.0
69	7602.5
70	5741.0
71	4171.5
72	3197.5
73	2426.0
74	1763.5
75	1191.0
76	705.0
77	441.5
78	290.0
79	171.5
80	107.0
81	54.0
82	25.0
83	12.5
84	6.0
85	2.5
86	1.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.045208038025183216
2	0.24639281282251252
3	0.0027316941967408187
4	0.00237147078618159
5	1.801117052796144E-4
6	9.00558526398072E-5
7	0.0
8	3.302047930126264E-4
9	0.0
10-11	5.703537333854457E-4
12-13	0.0
14-15	1.9512101405291563E-4
16-17	1.801117052796144E-4
18-19	5.553444246121445E-4
20-21	3.00186175466024E-5
22-23	3.00186175466024E-4
24-25	0.0013208191720505056
26-27	1.9512101405291563E-4
28-29	3.00186175466024E-4
30-31	1.0506516141310841E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.200744701864096E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.401489403728192E-4
70-71	1.50093087733012E-5
72-73	3.00186175466024E-4
74-75	1.3508377895971082E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	9.00558526398072E-5
84-85	0.0
86-87	5.253258070655421E-4
88-89	0.0021163125370354694
90-91	0.0019812287580757587
92-93	5.703537333854457E-4
94-95	5.253258070655421E-4
96-97	2.2513963159951803E-4
98-99	1.200744701864096E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3331266.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.97435522891605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.86602546097426	46.25881986263434
2	14.261785336396862	17.392063306000164
3	4.538129338499123	8.301285310812338
4	1.9182755177658408	4.678624513887489
5	0.9958994523102248	3.036216348872329
6	0.5974044687427856	2.185581137547869
7	0.38619242273195764	1.6483483779261872
8	0.26299715728324774	1.2828865673907084
9	0.18721839674805735	1.027396892583377
>10	0.9193871764301222	9.741219693516953
>50	0.04843745886485767	2.021169792525396
>100	0.017361818205652906	1.7195275626312667
>500	6.891072607426056E-4	0.2583798928529102
>1k	9.844389316536017E-5	0.11512896485181727
>5k	9.844389316536017E-5	0.3333517759669277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6077	0.1824231388307028	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5030	0.15099364625941009	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0018617546602403E-5	0.0
3	0.0	0.0	0.0	3.0018617546602403E-5	0.0
4	0.0	0.0	0.0	6.0037235093204806E-5	0.0
5	0.0	0.0	0.0	6.0037235093204806E-5	0.0
6	0.0	0.0	0.0	6.0037235093204806E-5	0.0
7	0.0	0.0	0.0	6.0037235093204806E-5	0.0
8	0.0	0.0	0.0	9.00558526398072E-5	0.0
9	0.0	0.0	0.0	1.801117052796144E-4	0.0
10-11	0.0	0.0	0.0	1.951210140529156E-4	0.0
12-13	0.0	0.0	0.0	2.4014894037281922E-4	0.0
14-15	0.0	0.0	0.0	3.00186175466024E-4	0.0
16-17	0.0	0.0	0.0	3.00186175466024E-4	0.0
18-19	0.0	0.0	0.0	3.00186175466024E-4	0.0
20-21	0.0	0.0	0.0	3.00186175466024E-4	0.0
22-23	0.0	0.0	0.0	3.3020479301262644E-4	0.0
24-25	0.0	0.0	0.0	3.3020479301262644E-4	0.0
26-27	0.0	0.0	0.0	4.5027926319903607E-4	0.0
28-29	0.0	0.0	0.0	8.705399088514696E-4	0.0
30-31	0.0	0.0	0.0	0.0032270013862597583	0.0
32-33	0.0	1.5009308773301202E-5	0.0	0.008465250148141878	0.0
34-35	0.0	3.0018617546602403E-5	0.0	0.015309494948767226	0.0
36-37	0.0	3.0018617546602403E-5	0.0	0.028787854227191705	0.0
38-39	0.0	3.0018617546602403E-5	0.0	0.0525625993241008	0.0
40-41	0.0	3.0018617546602403E-5	0.0	0.08319659853040856	0.0
42-43	0.0	3.0018617546602403E-5	0.0	0.11471614695434108	0.0
44-45	0.0	3.0018617546602403E-5	0.0	0.150093087733012	0.0
46-47	0.0	3.0018617546602403E-5	0.0	0.19301971082465347	0.0
48-49	0.0	3.0018617546602403E-5	0.0	0.24562733807507414	0.0
50-51	0.0	3.0018617546602403E-5	0.0	0.30008111030461093	0.0
52-53	0.0	6.0037235093204806E-5	0.0	0.35860240521171227	0.0
54-55	0.0	6.0037235093204806E-5	0.0	0.4215814648244841	0.0
56-57	0.0	6.0037235093204806E-5	0.0	0.4970632786454159	0.0
58-59	0.0	6.0037235093204806E-5	0.0	0.5839821857516032	0.0
60-61	0.0	6.0037235093204806E-5	0.0	0.6763194533249521	0.0
62-63	0.0	6.0037235093204806E-5	0.0	0.7729043552811454	0.0
64-65	0.0	6.0037235093204806E-5	0.0	0.8788400566031053	0.0
66-67	0.0	6.0037235093204806E-5	0.0	0.9976087169262376	0.0
68-69	0.0	6.0037235093204806E-5	0.0	1.1353191249212762	0.0
70-71	0.0	6.0037235093204806E-5	0.0	1.2855923243595677	0.0
72-73	0.0	6.0037235093204806E-5	0.0	1.4471675333041554	0.0
74-75	0.0	6.0037235093204806E-5	0.0	1.6100335428032466	0.0
76-77	0.0	6.0037235093204806E-5	0.0	1.785057092408712	0.0
78-79	0.0	6.0037235093204806E-5	0.0	1.9885683100659028	0.0
80-81	0.0	6.0037235093204806E-5	0.0	2.216139449686696	0.0
82-83	0.0	6.0037235093204806E-5	0.0	2.4569187810279933	0.0
84-85	0.0	6.0037235093204806E-5	0.0	2.6943060085865254	0.0
86-87	0.0	6.0037235093204806E-5	0.0	2.945531818834041	0.0
88	0.0	6.0037235093204806E-5	0.0	3.152495177509091	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5030	0.0	52.29174	1
GTATCAA	8710	0.0	42.862144	1
TCAACGC	10530	0.0	34.902714	4
ATCAACG	10575	0.0	34.755756	3
CAACGCA	10765	0.0	34.18342	5
TATCAAC	11095	0.0	33.546284	2
AACGCAG	11100	0.0	33.151756	6
ACGCAGA	12820	0.0	28.520643	7
CGCAGAG	12905	0.0	28.151125	8
GTACATG	9135	0.0	25.800514	1
GCAGAGT	14330	0.0	24.95817	9
TACATGG	9410	0.0	24.132488	2
ACATGGG	9155	0.0	23.410294	3
TACACCG	525	0.0	22.379402	5
GAGTACT	9810	0.0	21.678394	12-13
AGAGTAC	13155	0.0	21.41769	10-11
GTGGTAT	2080	0.0	20.849293	1
CAGAGTA	14270	0.0	20.748697	10-11
TAGACCG	210	9.545329E-7	20.14146	5
AGTACTT	10375	0.0	19.682453	12-13
>>END_MODULE