FastQCFastQC Report
Wed 25 May 2016
SRR1033100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66761
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11881.7794820329234133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4330.6485822561076078No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2790.41790865924716525No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2100.3145549048096943No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1380.20670750887494196No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1320.19772022588037927No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1250.1872350623867228No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1220.18274142088944145No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1130.1692604963975974No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1060.15877533290394094No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1050.15727745240484714No Hit
GTATAAATCATTGGCATTCGGAGTTTGTCTGAATTCGGTAATCCGGGATG960.1437965279130031No Hit
CTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCTTTTGACTACGGAT880.13181348392025283No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC850.12731984242297148No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.11683467892931501No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG750.11234103743203366No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA740.11084315693293989No Hit
GGTCTGGGCTGTTTCCCTTTTGACTACGGATCTTATCACTCGCAGTCTGA730.1093452764338461No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA730.1093452764338461No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT720.10784739593475232No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC710.10634951543565854No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC710.10634951543565854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.1018558739383772No Hit
GTACATGGGGGAGGCCCGAACCCACGTACGTTAAAAAGTGCGGGGATGAG670.10035799343928341No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC670.10035799343928341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCT200.002155469570.4882057
GAGAACA1000.065.788991
TACAAGA352.9198802E-453.7052962
GCGCGTC405.621973E-447.0273638
GTACAAA902.4374458E-1046.9921381
GGCGCGT405.642809E-446.9921347
GGAATCA1300.043.3773541
CGCGTCT450.001004213541.8020979
TAGAGCT450.001007928641.7707864
GCTGAAA450.001007928641.7707861
TAACAAC952.0691004E-839.5723231
AGAACAA1101.7462298E-938.4481122
GTAACAA500.001691959437.5937081
ATCAACA2300.036.7764553
TGGAAAC651.400659E-436.1477932
TCAACAC2250.033.416634
GAATCAA1755.456968E-1232.223182
ACATGTA300.004120968731.37509328-29
GAGGTAT300.00414373131.33982714-15
GGGACCT600.00413660831.3280895
Produced by FastQC (version 0.11.5)