##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033100_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66761 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.220053624121867 31.0 22.0 34.0 7.0 34.0 2 26.149458516199577 31.0 24.0 34.0 7.0 34.0 3 26.285301298662393 31.0 23.0 34.0 7.0 34.0 4 28.69939036263687 35.0 23.0 37.0 7.0 37.0 5 29.667844999325954 35.0 30.0 37.0 7.0 37.0 6 29.169200581177634 37.0 27.0 37.0 7.0 37.0 7 29.51891074130106 37.0 30.0 37.0 7.0 37.0 8 28.816404787226077 37.0 26.0 37.0 7.0 37.0 9 29.642171327571486 38.0 21.0 39.0 7.0 39.0 10-11 30.16615239436198 38.0 27.0 39.0 4.5 39.0 12-13 30.124002037117478 38.0 26.5 39.0 2.0 39.0 14-15 31.094306556222946 39.0 27.0 41.0 2.0 41.0 16-17 31.351942002067076 39.0 29.0 41.0 2.0 41.0 18-19 31.425016102215366 39.0 30.0 41.0 2.0 41.0 20-21 31.53925195847875 39.0 30.0 41.0 2.0 41.0 22-23 31.48741780380761 39.0 30.0 41.0 2.0 41.0 24-25 31.422454726561917 38.5 29.5 41.0 2.0 41.0 26-27 30.914238852024386 38.0 29.0 41.0 2.0 41.0 28-29 30.45449439043753 38.0 28.5 41.0 2.0 41.0 30-31 30.084300714488997 38.0 27.0 41.0 2.0 41.0 32-33 29.591101091954883 38.0 26.5 41.0 2.0 41.0 34-35 29.09760189332095 38.0 23.0 41.0 2.0 41.0 36-37 28.6089258698941 38.0 7.5 41.0 2.0 41.0 38-39 28.21274396728629 38.0 2.0 41.0 2.0 41.0 40-41 27.96417069846168 38.0 2.0 41.0 2.0 41.0 42-43 27.674181033837122 38.0 2.0 41.0 2.0 41.0 44-45 27.495626188942644 37.5 2.0 41.0 2.0 41.0 46-47 27.236417968574468 37.0 2.0 41.0 2.0 41.0 48-49 26.981254025553838 37.0 2.0 41.0 2.0 41.0 50-51 26.74943455011159 36.0 2.0 40.5 2.0 41.0 52-53 26.543775557586017 36.0 2.0 40.0 2.0 41.0 54-55 26.35523733916508 35.0 2.0 40.0 2.0 41.0 56-57 26.12229445334851 35.0 2.0 40.0 2.0 41.0 58-59 25.860502389119397 35.0 2.0 40.0 2.0 41.0 60-61 25.580997887988495 35.0 2.0 39.5 2.0 41.0 62-63 25.30996390107997 35.0 2.0 39.0 2.0 41.0 64-65 25.015398211530684 35.0 2.0 39.0 2.0 41.0 66-67 24.694949146957057 34.5 2.0 38.0 2.0 40.5 68-69 24.368501070984557 34.0 2.0 37.0 2.0 40.0 70-71 24.04268959422417 34.0 2.0 37.0 2.0 39.5 72-73 23.74262668324321 34.0 2.0 36.0 2.0 39.0 74-75 23.503392699330448 34.0 2.0 35.5 2.0 39.0 76-77 23.24452899147706 33.0 2.0 35.0 2.0 37.5 78-79 22.96295741525741 33.0 2.0 35.0 2.0 37.0 80-81 22.721214481508667 33.0 2.0 35.0 2.0 36.5 82-83 22.610730815895508 33.0 2.0 35.0 2.0 36.0 84-85 22.492375788259615 33.0 2.0 35.0 2.0 36.0 86-87 22.42828148170339 33.0 2.0 35.0 2.0 35.5 88-89 22.264915145069725 33.0 2.0 35.0 2.0 35.0 90-91 22.09078653705007 33.0 2.0 35.0 2.0 35.0 92-93 21.870350953400937 32.0 2.0 35.0 2.0 35.0 94-95 21.6961998771738 32.0 2.0 35.0 2.0 35.0 96-97 21.50533994397927 32.0 2.0 35.0 2.0 35.0 98-99 21.38039424214736 32.0 2.0 35.0 2.0 35.0 100 21.243390602297747 32.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3954676636642809 1101 2 -0.3343647793475242 1101 3 0.21696059487093322 1101 4 -0.9744007412797515 1101 5 -0.5100445471795467 1101 6 0.12263904132197112 1101 7 0.38142122524895683 1101 8 -1.0343565212553791 1101 9 -1.2181181877884448 1101 10-11 -0.28866272946755345 1101 12-13 -0.7742524477436206 1101 14-15 -0.32390748168496586 1101 16-17 -0.2643161010315431 1101 18-19 -0.6375341324322257 1101 20-21 -0.33275747491248353 1101 22-23 -0.5232550554067288 1101 24-25 -0.24991783149747704 1101 26-27 -0.04814726704368155 1101 28-29 -0.1413640407317054 1101 30-31 -0.4953847998302301 1101 32-33 -1.0531568492259424 1101 34-35 -1.4511144065287986 1101 36-37 -1.4215461904551 1101 38-39 -0.9378157987979172 1101 40-41 -0.5712266506814068 1101 42-43 -0.3534680910708623 1101 44-45 -0.45451430149145366 1101 46-47 -0.7080608833538875 1101 48-49 -0.9441490648060764 1101 50-51 -1.6306322493986407 1101 52-53 -1.726651679314294 1101 54-55 -1.3577886482433321 1101 56-57 -1.7069246317752018 1101 58-59 -1.688620801094423 1101 60-61 -1.8677355225470684 1101 62-63 -1.6132826307465784 1101 64-65 -1.5435140015308058 1101 66-67 -1.5345495302730505 1101 68-69 -1.5809476970798322 1101 70-71 -1.6002404069028522 1101 72-73 -1.4609043176112877 1101 74-75 -1.543883540413102 1101 76-77 -1.1422927675037258 1101 78-79 -1.1305432730861504 1101 80-81 -1.5639006565934395 1101 82-83 -1.6077799204970233 1101 84-85 -1.3236287361435828 1101 86-87 -1.1085068412105912 1101 88-89 -1.2749854349251528 1101 90-91 -1.440562331885829 1101 92-93 -1.4439532072203534 1101 94-95 -1.4944980243670152 1101 96-97 -1.3961457364900838 1101 98-99 -1.5734921660057601 1101 100 -1.2423418990017723 1102 1 1.8708534346825942 1102 2 2.087742253434765 1102 3 2.607406097812934 1102 4 2.095786986375053 1102 5 2.105393950999215 1102 6 2.576547699209332 1102 7 2.2406254841450064 1102 8 3.2011436188398896 1102 9 2.432344126585331 1102 10-11 2.3611691562147072 1102 12-13 2.103654529000565 1102 14-15 2.795944017334371 1102 16-17 2.8429968717899747 1102 18-19 2.6923201684924045 1102 20-21 3.1098735141600926 1102 22-23 3.269544047983576 1102 24-25 3.069989705627762 1102 26-27 2.755649314631853 1102 28-29 2.293911946939911 1102 30-31 2.2368231015427007 1102 32-33 2.6954421981262584 1102 34-35 2.793351830512389 1102 36-37 3.2060105481271357 1102 38-39 3.5399791017344455 1102 40-41 3.538537989273763 1102 42-43 2.7410962126556697 1102 44-45 2.876530809183805 1102 46-47 2.604911938164747 1102 48-49 1.9130938603830536 1102 50-51 2.438925049564652 1102 52-53 2.4591146417840513 1102 54-55 2.0503088580609585 1102 56-57 1.9263059650306253 1102 58-59 2.3884315945346053 1102 60-61 2.2650047408320297 1102 62-63 2.338314455271913 1102 64-65 2.0997334067067968 1102 66-67 2.066809393795314 1102 68-69 2.061809377731038 1102 70-71 1.7517489458570203 1102 72-73 2.065923925594088 1102 74-75 1.843409819071347 1102 76-77 2.1283012364189418 1102 78-79 2.046046808169102 1102 80-81 2.0530088418655126 1102 82-83 1.7095358542297916 1102 84-85 0.9991507539096531 1102 86-87 1.5230426124178784 1102 88-89 1.5915738253718494 1102 90-91 1.4572381724571244 1102 92-93 1.9170022424854487 1102 94-95 2.2597748812087772 1102 96-97 1.9388920892314054 1102 98-99 2.3997496384212518 1102 100 1.443704612626135 1103 1 1.387830433587304 1103 2 0.3471891318575473 1103 3 0.7896350134755856 1103 4 1.4587387936039704 1103 5 1.6294903365413838 1103 6 -0.08322776628902417 1103 7 -0.05277539208719517 1103 8 0.6622608148968432 1103 9 -0.09681797637195189 1103 10-11 1.2023520696867784 1103 12-13 1.4502454380914749 1103 14-15 0.6708071060528766 1103 16-17 0.950403772118559 1103 18-19 1.0281635419863768 1103 20-21 1.6294518274130994 1103 22-23 0.9479394477645151 1103 24-25 0.7553675807646769 1103 26-27 0.33279882175123987 1103 28-29 -0.31089363904037626 1103 30-31 -0.5391215862995757 1103 32-33 -0.39697291687921776 1103 34-35 -0.4097560555774251 1103 36-37 -0.6892523215333242 1103 38-39 -0.9219595620114447 1103 40-41 -0.8826695682289767 1103 42-43 -0.8674744335655795 1103 44-45 -1.1835047877493814 1103 46-47 -1.0790968241572685 1103 48-49 -0.8846881768568124 1103 50-51 -0.6061872176861662 1103 52-53 -0.5480047448534044 1103 54-55 -0.3530317772073879 1103 56-57 -0.38728932522129256 1103 58-59 -0.1821461710732848 1103 60-61 -0.4303676578536191 1103 62-63 -0.35746867725820763 1103 64-65 -0.16957108398323584 1103 66-67 -0.028207035558462934 1103 68-69 -0.19722676684727602 1103 70-71 0.0022965909829792963 1103 72-73 -0.2808038947783089 1103 74-75 -0.3101361831192335 1103 76-77 -0.6154693002732792 1103 78-79 -0.47264158175422466 1103 80-81 -0.15256344729111504 1103 82-83 -0.3186678697570642 1103 84-85 -0.5322703851922128 1103 86-87 -0.3787235431133418 1103 88-89 0.13846593928203532 1103 90-91 0.1672600782621636 1103 92-93 -0.15008428544444996 1103 94-95 0.24334552743002646 1103 96-97 -1.076510429936171 1103 98-99 -0.9733336036378937 1103 100 -0.5676590237375017 1104 1 1.0463928014520008 1104 2 0.8516817322803831 1104 3 1.7402164088244234 1104 4 1.3046690261621094 1104 5 1.1062345225878971 1104 6 1.1807785762056895 1104 7 1.113979364783841 1104 8 0.8609923159539257 1104 9 2.1423469284906282 1104 10-11 1.9438954100673271 1104 12-13 2.18504987783777 1104 14-15 2.362139029942938 1104 16-17 2.1077769222242715 1104 18-19 2.007814704777079 1104 20-21 2.149800664622397 1104 22-23 2.3197682004072284 1104 24-25 2.3140356568746085 1104 26-27 2.515806221328411 1104 28-29 2.428403401128751 1104 30-31 2.2894989210999945 1104 32-33 2.6387036158903356 1104 34-35 2.397722802773526 1104 36-37 2.526128228149549 1104 38-39 2.323812108178828 1104 40-41 2.533424512109292 1104 42-43 2.6697877228826243 1104 44-45 2.2722298845550597 1104 46-47 2.2396135352507685 1104 48-49 2.3465486096125296 1104 50-51 2.28215844827578 1104 52-53 2.2210227392903583 1104 54-55 2.3340718168729495 1104 56-57 2.2581916472945665 1104 58-59 2.4102164082079014 1104 60-61 2.4636598262901437 1104 62-63 2.241368532044117 1104 64-65 2.1076487891668663 1104 66-67 2.145683027866486 1104 68-69 1.8260290471062142 1104 70-71 1.8009223837948163 1104 72-73 1.853049170760805 1104 74-75 1.9212327386566663 1104 76-77 2.3228235115660425 1104 78-79 2.3927125408673398 1104 80-81 2.1860993434065605 1104 82-83 2.328266591130884 1104 84-85 2.10660382199595 1104 86-87 2.391493158789409 1104 88-89 2.3819913092608935 1104 90-91 2.390833016951383 1104 92-93 2.0676746997563953 1104 94-95 2.208990347726008 1104 96-97 2.2375751937424724 1104 98-99 2.5776706246919154 1104 100 2.606495310300552 1105 1 1.207011252768126 1105 2 0.8408205004889169 1105 3 0.3487009475415199 1105 4 0.13868384854902516 1105 5 0.21740242445347135 1105 6 0.11395005588880025 1105 7 1.4855612712494661 1105 8 1.1387842914203183 1105 9 0.38282737827340085 1105 10-11 0.2253290926638023 1105 12-13 0.17508310042913422 1105 14-15 -0.7515205662747917 1105 16-17 0.05423743845222617 1105 18-19 0.17239431121714688 1105 20-21 0.6280782010394788 1105 22-23 0.676336051161119 1105 24-25 1.3150578904549803 1105 26-27 1.6310630575154832 1105 28-29 1.3196633040165686 1105 30-31 1.0801965955186041 1105 32-33 0.7997054047991021 1105 34-35 1.06370798038661 1105 36-37 0.6263071190261158 1105 38-39 0.9219465318946476 1105 40-41 1.49451574645634 1105 42-43 1.5247583336671902 1105 44-45 1.6814427646981684 1105 46-47 2.103720358659917 1105 48-49 1.600701433532798 1105 50-51 1.444949145950197 1105 52-53 1.282165085316425 1105 54-55 1.3844807103050982 1105 56-57 1.5191167686848033 1105 58-59 1.0440651176380058 1105 60-61 0.9396373371513747 1105 62-63 0.013410443620912815 1105 64-65 0.2973370079248525 1105 66-67 0.39561913826770834 1105 68-69 0.363212793592286 1105 70-71 0.27127760996399886 1105 72-73 0.034942858468443916 1105 74-75 0.2190221585391079 1105 76-77 0.31815957085559177 1105 78-79 0.7457025740899326 1105 80-81 0.8646068823792135 1105 82-83 0.838425037335842 1105 84-85 0.32306178263995733 1105 86-87 0.5266835497937521 1105 88-89 0.21907283238892816 1105 90-91 0.3942830552851433 1105 92-93 0.3461694608092927 1105 94-95 0.2563325404170378 1105 96-97 0.6678077518820089 1105 98-99 1.196121940817651 1105 100 1.2678804368019598 1106 1 0.15391827832683447 1106 2 0.31692866199033887 1106 3 0.01014063145311539 1106 4 0.398345535177004 1106 5 0.2643217972155405 1106 6 0.10382534913487973 1106 7 -0.9704209487776971 1106 8 0.026918628992859794 1106 9 -1.1393358517549963 1106 10-11 -0.27109021836225367 1106 12-13 -0.1266825496511217 1106 14-15 0.053084940577903694 1106 16-17 -0.16214730040442404 1106 18-19 0.22338826108750354 1106 20-21 0.10838441168275992 1106 22-23 0.18683117401579707 1106 24-25 0.5753369372508885 1106 26-27 0.9325817635285674 1106 28-29 0.3291089145857704 1106 30-31 0.41727524720399956 1106 32-33 0.19253167964784978 1106 34-35 0.5680912375788552 1106 36-37 1.0718559522153974 1106 38-39 1.184015923177519 1106 40-41 0.2814647525064693 1106 42-43 0.8111517155661865 1106 44-45 0.6131627301259996 1106 46-47 0.3652341257916589 1106 48-49 0.6552760723770987 1106 50-51 0.7033761122423314 1106 52-53 0.7742628751670217 1106 54-55 1.0987046885740206 1106 56-57 1.2418603172710476 1106 58-59 0.9810943805099654 1106 60-61 1.216338164414001 1106 62-63 1.4599470530788707 1106 64-65 0.9633451570790719 1106 66-67 1.0619359675058888 1106 68-69 1.2915372780965448 1106 70-71 1.2793650281637774 1106 72-73 1.4466603544556058 1106 74-75 1.420775461416003 1106 76-77 1.4143442850178332 1106 78-79 1.7216255275409722 1106 80-81 1.652914080798773 1106 82-83 1.3153975030723544 1106 84-85 1.0607454472899143 1106 86-87 1.0890369267538738 1106 88-89 1.1865379515394388 1106 90-91 1.334522591030293 1106 92-93 1.1407737329416534 1106 94-95 1.2402811760510666 1106 96-97 1.0273583136797626 1106 98-99 0.37842839840500275 1106 100 1.0055023654351238 1107 1 1.191007838706458 1107 2 0.45983255639417564 1107 3 0.9389068581357805 1107 4 0.6565543275845513 1107 5 1.2751214045025492 1107 6 1.7683830015838815 1107 7 2.0049254022640817 1107 8 1.204184910218995 1107 9 1.4491882019157778 1107 10-11 1.70191076342023 1107 12-13 1.3706448202627008 1107 14-15 1.6498450583917972 1107 16-17 1.3653971525245616 1107 18-19 1.077920823539774 1107 20-21 0.8418304681897979 1107 22-23 1.0199826957786406 1107 24-25 1.3394364245422565 1107 26-27 1.427072872942766 1107 28-29 2.2075860608713604 1107 30-31 1.4491286343535563 1107 32-33 2.1320429701463723 1107 34-35 1.9633132295064222 1107 36-37 2.306665595501098 1107 38-39 1.8836450275737278 1107 40-41 2.157323812177026 1107 42-43 2.4671460430451866 1107 44-45 2.074273235198543 1107 46-47 2.2527654882875687 1107 48-49 2.1041688399128233 1107 50-51 2.2507743886686455 1107 52-53 2.3668231457026394 1107 54-55 2.3563680462701058 1107 56-57 2.417708468247376 1107 58-59 1.7928648615833822 1107 60-61 1.961401980275241 1107 62-63 1.979909963518491 1107 64-65 1.8590034967758093 1107 66-67 2.047918295421237 1107 68-69 1.9629674079100035 1107 70-71 2.0448826457049556 1107 72-73 1.7984657433505546 1107 74-75 1.9740098892549938 1107 76-77 1.9602698877231894 1107 78-79 1.7491699804699579 1107 80-81 1.8067812129030614 1107 82-83 1.724800797498009 1107 84-85 1.9742940455227043 1107 86-87 1.640307789631585 1107 88-89 1.6013410496086031 1107 90-91 1.5973130350141496 1107 92-93 1.3285123606279186 1107 94-95 1.5482881576153744 1107 96-97 0.9930504703286118 1107 98-99 0.556785549054073 1107 100 0.34661723398535926 1108 1 -2.8187008991576192 1108 2 -2.7149247251592215 1108 3 -2.0902193554564548 1108 4 -4.158979652997971 1108 5 -3.0064319935557045 1108 6 -1.2413257362801957 1108 7 -2.791191102590286 1108 8 -2.6501840218198396 1108 9 -3.308093351482281 1108 10-11 -2.7349824404632663 1108 12-13 -2.8720736263392403 1108 14-15 -3.1899607668509162 1108 16-17 -2.9161562455336885 1108 18-19 -2.5774189822854687 1108 20-21 -2.556227784237386 1108 22-23 -2.868366818784466 1108 24-25 -2.5197868764286184 1108 26-27 -2.45156790375626 1108 28-29 -2.8312488905849484 1108 30-31 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2216 9 0.6604444979957833 2216 10-11 0.6674921927216388 2216 12-13 0.8442560327599651 2216 14-15 0.5093238524643624 2216 16-17 0.7792229792272387 2216 18-19 0.7753439931141948 2216 20-21 0.9612187447063292 2216 22-23 0.9837391743537189 2216 24-25 1.1687305336013196 2216 26-27 1.66084906098569 2216 28-29 1.7997095735365214 2216 30-31 1.8621514827366532 2216 32-33 1.6178083544809994 2216 34-35 1.5960966443542226 2216 36-37 1.5080306586443903 2216 38-39 1.889557867927035 2216 40-41 1.6914503907594067 2216 42-43 0.988899455703045 2216 44-45 1.1860697166935417 2216 46-47 1.3648539618987812 2216 48-49 1.7585961703749007 2216 50-51 1.7193852361757607 2216 52-53 1.408249527190339 2216 54-55 1.3026414968869382 2216 56-57 1.219810811484109 2216 58-59 1.380097506301972 2216 60-61 1.3520144337389972 2216 62-63 1.1744862099598343 2216 64-65 0.7501999344719934 2216 66-67 0.3291067033342223 2216 68-69 0.4456303760098663 2216 70-71 0.26665065796862564 2216 72-73 0.5537398509496505 2216 74-75 0.1846092030735207 2216 76-77 0.13568415153806512 2216 78-79 -0.08136509508300094 2216 80-81 -0.002657432253521108 2216 82-83 -0.11993239470580264 2216 84-85 -0.13731994089965838 2216 86-87 -0.3345078903450549 2216 88-89 -0.09440316529759585 2216 90-91 0.3318192033476066 2216 92-93 0.2776327343778284 2216 94-95 0.04204682613132249 2216 96-97 0.3520182781977965 2216 98-99 0.1605522473532197 2216 100 -0.15779914677236206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6065.0 3 684.0 4 499.0 5 528.0 6 1350.0 7 2767.0 8 3083.0 9 1664.0 10 928.0 11 993.0 12 888.0 13 583.0 14 462.0 15 373.0 16 347.0 17 312.0 18 276.0 19 217.0 20 166.0 21 180.0 22 234.0 23 469.0 24 795.0 25 637.0 26 489.0 27 409.0 28 474.0 29 521.0 30 608.0 31 804.0 32 975.0 33 1391.0 34 1848.0 35 3452.0 36 5887.0 37 10535.0 38 11834.0 39 3026.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.84985988521077 20.897334072619923 13.016439136233535 17.23636690593577 2 17.621673459602015 28.846499640374013 32.16105250539439 21.370774394629585 3 21.557666946409306 35.027274907085484 24.598369500059945 18.81668864644527 4 12.7107846811062 25.154762796972197 29.966274451023008 32.1681780708986 5 12.401996791940876 47.34735485031556 25.98677799931042 14.263870358433147 6 25.09931491447674 42.26842760130121 14.47074519915451 18.161512285067534 7 22.370137171126604 45.77467955925343 15.721460160407766 16.1337231092122 8 21.60431816477997 42.00464802458955 16.632431216732886 19.758602593897592 9 20.614692653673163 30.712143928035985 15.833583208395801 32.839580209895054 10-11 19.865817091454275 39.34632683658171 19.733883058470763 21.053973013493255 12-13 22.11327261141722 39.72712620413059 19.635668503317216 18.523932681134976 14-15 20.779542080878052 39.92772854722384 19.37684614577242 19.91588322612568 16-17 18.60892113072934 41.329176534068765 20.558170208939117 19.503732126262776 18-19 19.335057535889653 39.882304434199185 22.24895985606657 18.5336781738446 20-21 22.863353571696273 36.98073607675587 21.284011693276366 18.871898658271494 22-23 21.52657569558408 37.15576737180496 21.07022158277694 20.24743534983402 24-25 21.697378277153557 37.411235955056185 20.628464419475655 20.26292134831461 26-27 21.183681430800625 36.59224582883207 22.20246669067339 20.02160604969391 28-29 20.654332366671913 38.33749222549439 22.021146655276546 18.987028752557155 30-31 20.46783187432546 38.840088739657034 21.88361913898549 18.80846024703202 32-33 19.705201315878213 39.236550840408555 22.795566779320627 18.262681064392606 34-35 19.60299625468165 38.98576779026217 21.683895131086143 19.72734082397004 36-37 19.019503532814337 39.209368888755684 21.859991158598262 19.911136419831713 38-39 18.83328087294093 38.218145300298275 22.800785407017703 20.147788419743094 40-41 18.831835590565348 38.706220067004935 23.053746355575875 19.40819798685384 42-43 17.657815650036348 38.8125697926269 22.89872668270492 20.63088787463183 44-45 17.999370494162083 38.33765494087142 23.064644254260404 20.598330310706096 46-47 18.632888622395445 38.2401439064608 22.651026832558838 20.47594063858492 48-49 18.004541605150152 36.96536838713062 22.32281369676167 22.707276310957557 50-51 17.781691460261552 36.219882339715966 22.509836249859482 23.488589950163 52-53 18.690559165411337 35.07955422652907 21.62615883865069 24.60372776940891 54-55 18.18202255610926 34.11367979317322 21.86293978792761 25.841357862789916 56-57 20.066543770513913 33.54015856601172 21.11415853603705 25.27913912743732 58-59 20.985424706808047 32.39686762336543 21.640376184945108 24.97733148488141 60-61 20.172349194454853 31.97227426002248 21.959535406519297 25.895841139003373 62-63 20.676708633093526 32.29691247002398 21.541516786570742 25.484862110311752 64-65 21.609489980966085 32.2699818653238 21.63197098451808 24.48855716919203 66-67 22.358107399247636 31.627025163736644 21.223566086656774 24.791301350358946 68-69 23.547968917430367 31.258664228818066 21.93164532300245 23.261721530749114 70-71 24.42677736482436 31.438076969188344 21.726261839108023 22.408883826879272 72-73 24.668609922594474 30.078004990521006 23.366278764808584 21.887106322075937 74-75 24.432754848185116 30.056499715253427 23.586008452477294 21.924736984084163 76-77 25.534433796147134 28.81034624866064 23.42367318802029 22.23154676717194 78-79 26.710973910176676 27.787684884086854 24.382970433531643 21.118370772204823 80-81 27.286690442652922 27.199028980729462 24.33242425150598 21.181856325111635 82-83 26.885756887779948 27.191464173054303 24.88067675200995 21.0421021871558 84-85 26.67730552246522 27.206251451604444 24.820001048901275 21.296441977029062 86-87 26.41768121371043 27.264281700693015 24.77168008990448 21.546356995692076 88-89 26.690042180815592 27.152307957414607 24.02882980078368 22.12882006098612 90-91 27.648001917919057 26.460540313759573 23.644345884715083 22.247111883606287 92-93 27.230296673658977 27.73224453101588 23.620017980221757 21.417440815103387 94-95 25.622199913094292 28.643671616296317 23.648841007506853 22.085287463102535 96-97 24.087088122031258 29.97063098431155 23.670527593388975 22.27175330026822 98-99 22.952440888249573 32.32191555036111 22.40252929365579 22.323114267733526 100 21.97263722596017 33.43173542325386 22.141968741102602 22.453658609683366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 786.0 1 481.5 2 173.5 3 145.5 4 117.5 5 90.0 6 66.0 7 67.5 8 65.5 9 71.5 10 61.5 11 39.5 12 39.5 13 40.5 14 42.0 15 45.0 16 41.5 17 48.5 18 62.5 19 84.0 20 104.5 21 132.5 22 193.5 23 265.0 24 325.5 25 407.5 26 535.0 27 640.5 28 776.0 29 923.5 30 1009.5 31 1145.0 32 1311.0 33 1488.5 34 1633.5 35 1747.0 36 1923.0 37 2010.0 38 1950.0 39 1908.5 40 1881.0 41 1783.0 42 1764.5 43 1697.0 44 1583.0 45 1574.5 46 1759.5 47 2287.5 48 3045.5 49 3339.5 50 2920.5 51 2519.5 52 2236.0 53 2036.0 54 1762.0 55 1514.5 56 1421.5 57 1287.0 58 1173.0 59 995.5 60 889.0 61 792.5 62 634.5 63 516.5 64 467.0 65 426.0 66 339.5 67 264.0 68 231.5 69 220.0 70 179.5 71 133.0 72 97.0 73 78.5 74 69.5 75 59.0 76 44.0 77 31.5 78 28.0 79 22.0 80 12.0 81 11.5 82 14.5 83 7.5 84 1.5 85 1.0 86 1.5 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04493641497281347 2 0.03744701247734456 3 0.04943005647009482 4 0.06890250295831399 5 0.08088554695106424 6 0.08088554695106424 7 0.08388130794925182 8 0.09886011294018963 9 0.09137071044472073 10-11 0.09137071044472073 12-13 0.09511541169245519 14-15 0.1018558739383772 16-17 0.06440886146103264 18-19 0.09511541169245519 20-21 0.08388130794925182 22-23 0.05467263821692305 24-25 0.016476685490031603 26-27 0.1692604963975974 28-29 0.05467263821692305 30-31 0.07339614445559534 32-33 0.05617051871601683 34-35 0.016476685490031603 36-37 0.04418747472326658 38-39 0.06590674196012641 40-41 0.07414508470514222 42-43 0.06815356270876709 44-45 0.06291098096193885 46-47 0.0763919054537829 48-49 0.06665568220967331 50-51 0.06515780171057953 52-53 0.06815356270876709 54-55 0.05766839921511062 56-57 0.056919458965563725 58-59 0.05766839921511062 60-61 0.05392369796737616 62-63 0.061413100462845066 64-65 0.056919458965563725 66-67 0.056919458965563725 68-69 0.05317475771782927 70-71 0.04943005647009482 72-73 0.05167687721873549 74-75 0.05242581746828238 76-77 0.04718323572145414 78-79 0.04493641497281347 80-81 0.04044277347553212 82-83 0.04568535522236036 84-85 0.03669807222779767 86-87 0.03520019172870388 88-89 0.03669807222779767 90-91 0.03295337098006321 92-93 0.03145549048096943 94-95 0.03295337098006321 96-97 0.035949131978250774 98-99 0.034451251479156994 100 0.04194065397462591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 66761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.19849912373991 #Duplication Level Percentage of deduplicated Percentage of total 1 87.7685042828904 59.85680262428663 2 6.356248627278717 8.669732328754812 3 2.1019108280254777 4.300414912898249 4 1.0608390072479685 2.8939051242491876 5 0.6171754886887766 2.104522101226764 6 0.4392708104546453 1.7974565989125386 7 0.29650779705688557 1.4154970716436244 8 0.26795519437733367 1.4619313671155316 9 0.13837030529321326 0.8492982429861745 >10 0.8807379749615638 9.935441350489057 >50 0.048319789150010985 2.2947529246116747 >100 0.021963540522732264 2.640763319902338 >500 0.0 0.0 >1k 0.0021963540522732267 1.7794820329234133 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1188 1.7794820329234133 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 433 0.6485822561076078 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 279 0.41790865924716525 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 210 0.3145549048096943 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 138 0.20670750887494196 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 132 0.19772022588037927 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 125 0.1872350623867228 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 122 0.18274142088944145 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 113 0.1692604963975974 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 106 0.15877533290394094 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 105 0.15727745240484714 No Hit GTATAAATCATTGGCATTCGGAGTTTGTCTGAATTCGGTAATCCGGGATG 96 0.1437965279130031 No Hit CTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCTTTTGACTACGGAT 88 0.13181348392025283 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 85 0.12731984242297148 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.11683467892931501 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 75 0.11234103743203366 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 74 0.11084315693293989 No Hit GGTCTGGGCTGTTTCCCTTTTGACTACGGATCTTATCACTCGCAGTCTGA 73 0.1093452764338461 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 73 0.1093452764338461 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 72 0.10784739593475232 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 71 0.10634951543565854 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 71 0.10634951543565854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.1018558739383772 No Hit GTACATGGGGGAGGCCCGAACCCACGTACGTTAAAAAGTGCGGGGATGAG 67 0.10035799343928341 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 67 0.10035799343928341 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0014978804990937824 0.0 0.0 0.0 0.0 9 0.0014978804990937824 0.0 0.0 0.0014978804990937824 0.0 10-11 0.002995760998187565 0.0 0.0 0.0014978804990937824 0.0 12-13 0.002995760998187565 0.0 0.0 0.002995760998187565 0.0 14-15 0.002995760998187565 0.0 0.0 0.002995760998187565 0.0 16-17 0.003744701247734456 0.0 0.0 0.003744701247734456 0.0 18-19 0.004493641497281347 0.0 0.0 0.004493641497281347 0.0 20-21 0.004493641497281347 0.0 0.0 0.004493641497281347 0.0 22-23 0.004493641497281347 0.0 0.0 0.009736223244109585 0.0 24-25 0.004493641497281347 0.0 0.0 0.02696184898368808 0.0 26-27 0.004493641497281347 0.0 0.0 0.058417339464657506 0.0 28-29 0.004493641497281347 0.0 0.0 0.13855394616617486 0.0 30-31 0.004493641497281347 0.0 0.0 0.30856338281331913 0.0 32-33 0.004493641497281347 0.0 0.0 0.5609562469106215 0.0 34-35 0.004493641497281347 0.0 0.0 0.9316816704363327 0.0 36-37 0.004493641497281347 0.0 0.0 1.4454546816254998 0.0 38-39 0.004493641497281347 0.0 0.0 2.1284881892122645 0.0 40-41 0.004493641497281347 0.0 0.0 2.7665852818262158 0.0 42-43 0.004493641497281347 0.0 0.0 3.2301792962957414 0.0 44-45 0.004493641497281347 0.0 0.0 3.6143856443132965 0.0 46-47 0.004493641497281347 0.0 0.0 4.074983897784635 0.0 48-49 0.004493641497281347 0.0 0.0 4.517607585266847 0.0 50-51 0.004493641497281347 0.0 0.0 4.959482332499514 0.0 52-53 0.004493641497281347 0.0 0.0 5.312982130285645 0.0 54-55 0.004493641497281347 0.0 0.0 5.724899267536436 0.0 56-57 0.004493641497281347 0.0 0.0 6.233429696978775 0.0 58-59 0.004493641497281347 0.0 0.0 6.637108491484549 0.0 60-61 0.004493641497281347 0.0 0.0 7.043783046988512 0.0 62-63 0.004493641497281347 0.0 0.0 7.416006351013316 0.0 64-65 0.004493641497281347 0.0 0.0 7.786731774539027 0.0 66-67 0.004493641497281347 0.0 0.0 8.242836386513083 0.0 68-69 0.004493641497281347 0.0 0.0 8.774583963691377 0.0 70-71 0.004493641497281347 0.0 0.0 9.34228067284792 0.0 72-73 0.004493641497281347 0.0 0.0 9.903236919758541 0.0 74-75 0.004493641497281347 0.0 0.0 10.44097601893321 0.0 76-77 0.004493641497281347 0.0 0.0 10.968978894863767 0.0 78-79 0.004493641497281347 0.0 0.0 11.601084465481343 0.0 80-81 0.004493641497281347 0.0 0.0 12.256407183834874 0.0 82-83 0.004493641497281347 0.0 0.0 12.896751097197466 0.0 84-85 0.004493641497281347 0.0 0.0 13.561810038795105 0.0 86-87 0.004493641497281347 0.0 0.0 14.252332948877338 0.0 88 0.004493641497281347 0.0 0.0 14.737646230583724 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTGCT 20 0.0021554695 70.488205 7 GAGAACA 100 0.0 65.78899 1 TACAAGA 35 2.9198802E-4 53.705296 2 GCGCGTC 40 5.621973E-4 47.027363 8 GTACAAA 90 2.4374458E-10 46.992138 1 GGCGCGT 40 5.642809E-4 46.992134 7 GGAATCA 130 0.0 43.377354 1 CGCGTCT 45 0.0010042135 41.802097 9 TAGAGCT 45 0.0010079286 41.770786 4 GCTGAAA 45 0.0010079286 41.770786 1 TAACAAC 95 2.0691004E-8 39.572323 1 AGAACAA 110 1.7462298E-9 38.448112 2 GTAACAA 50 0.0016919594 37.593708 1 ATCAACA 230 0.0 36.776455 3 TGGAAAC 65 1.400659E-4 36.147793 2 TCAACAC 225 0.0 33.41663 4 GAATCAA 175 5.456968E-12 32.22318 2 ACATGTA 30 0.0041209687 31.375093 28-29 GAGGTAT 30 0.004143731 31.339827 14-15 GGGACCT 60 0.004136608 31.328089 5 >>END_MODULE