##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033100_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66761
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33395245727296	33.0	31.0	34.0	30.0	34.0
2	32.29204176090831	33.0	31.0	34.0	30.0	34.0
3	32.29376432348227	34.0	31.0	34.0	30.0	34.0
4	35.95479396653735	37.0	35.0	37.0	35.0	37.0
5	35.986938482047904	37.0	35.0	37.0	35.0	37.0
6	36.11935111816779	37.0	35.0	37.0	35.0	37.0
7	36.05778822965504	37.0	35.0	37.0	35.0	37.0
8	36.10230523808811	37.0	36.0	37.0	35.0	37.0
9	37.795389523823786	39.0	38.0	39.0	35.0	39.0
10-11	37.75381585057144	39.0	38.0	39.0	35.0	39.0
12-13	37.69787750333278	39.0	38.0	39.0	35.0	39.0
14-15	38.857064753373976	40.0	38.0	41.0	35.0	41.0
16-17	38.89875825706625	40.0	38.0	41.0	35.5	41.0
18-19	39.150686778208836	40.5	39.0	41.0	36.0	41.0
20-21	39.193338925420534	41.0	39.0	41.0	36.0	41.0
22-23	39.00257635445844	41.0	38.5	41.0	35.0	41.0
24-25	38.832649301238746	41.0	38.0	41.0	35.0	41.0
26-27	38.47220682733932	40.0	38.0	41.0	34.5	41.0
28-29	38.21805395365558	40.0	37.5	41.0	34.0	41.0
30-31	37.64817782837285	39.5	36.5	41.0	33.5	41.0
32-33	36.879180958943095	39.0	35.0	41.0	33.0	41.0
34-35	36.265566723086835	39.0	35.0	41.0	31.5	41.0
36-37	35.749314719671666	39.0	35.0	41.0	30.0	41.0
38-39	35.11804047273108	38.5	35.0	41.0	27.0	41.0
40-41	34.69369092733781	38.5	35.0	41.0	23.5	41.0
42-43	34.04653165770435	38.5	35.0	41.0	10.0	41.0
44-45	32.94880244454097	38.0	34.0	41.0	2.0	41.0
46-47	31.319677656116596	38.0	32.0	41.0	2.0	41.0
48-49	29.900166264735397	38.0	26.0	41.0	2.0	41.0
50-51	29.115434160662662	38.0	18.5	41.0	2.0	41.0
52-53	28.8175207081979	37.0	10.5	41.0	2.0	41.0
54-55	28.577889785952877	37.0	6.5	40.5	2.0	41.0
56-57	28.317498239990414	36.5	2.0	40.0	2.0	41.0
58-59	28.032009706265633	36.0	2.0	40.0	2.0	41.0
60-61	27.721970911160707	35.0	2.0	40.0	2.0	41.0
62-63	27.421855574362276	35.0	2.0	39.0	2.0	41.0
64-65	26.966769521127603	35.0	2.0	39.0	2.0	41.0
66-67	26.326126031665193	35.0	2.0	38.5	2.0	41.0
68-69	25.631678674675335	35.0	2.0	37.0	2.0	40.0
70-71	24.867287787780292	35.0	2.0	37.0	2.0	39.5
72-73	24.359993109749702	34.5	2.0	36.0	2.0	39.0
74-75	24.102994263117687	34.0	2.0	36.0	2.0	39.0
76-77	23.839943979269336	34.0	2.0	35.0	2.0	37.5
78-79	23.564026901933765	34.0	2.0	35.0	2.0	37.0
80-81	23.354525845928013	34.0	2.0	35.0	2.0	36.5
82-83	23.139265439403246	34.0	2.0	35.0	2.0	36.0
84-85	23.019142912778417	34.0	2.0	35.0	2.0	36.0
86-87	22.938077620167462	34.0	2.0	35.0	2.0	35.5
88-89	22.82800587169156	34.0	2.0	35.0	2.0	35.0
90-91	22.649713155884424	34.0	2.0	35.0	2.0	35.0
92-93	22.502321714773593	33.0	2.0	35.0	2.0	35.0
94-95	22.353050433636405	33.0	2.0	35.0	2.0	35.0
96-97	22.238477554260722	33.0	2.0	35.0	2.0	35.0
98-99	22.132405146717396	33.0	2.0	35.0	2.0	35.0
100	22.008987282994564	33.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.11004680659690536
1101	2	0.18475638159207364
1101	3	0.3222606989145831
1101	4	0.19301870660253684
1101	5	0.4258611650519839
1101	6	0.2637466142227609
1101	7	0.173169111221803
1101	8	0.20191128583909546
1101	9	0.43952044229771303
1101	10-11	0.5425010539615869
1101	12-13	0.43111966597254536
1101	14-15	0.21111926127875336
1101	16-17	0.08323486857452167
1101	18-19	0.5193920241518555
1101	20-21	0.3266153331464565
1101	22-23	0.44371394183899326
1101	24-25	0.30146934598079156
1101	26-27	0.28877173294474545
1101	28-29	0.3140246059711487
1101	30-31	0.1150355973232422
1101	32-33	-0.36669444017641695
1101	34-35	-1.2559118863614032
1101	36-37	-1.434335797160422
1101	38-39	-1.0654603092808017
1101	40-41	-0.7845585615278239
1101	42-43	-1.3373877317625826
1101	44-45	-1.9942776119610635
1101	46-47	-1.8906240288467018
1101	48-49	-2.2786631626378906
1101	50-51	-2.861817553006027
1101	52-53	-2.6279260310023
1101	54-55	-2.6683739012677954
1101	56-57	-2.7668866126972986
1101	58-59	-2.4919287869741886
1101	60-61	-2.3038224672069703
1101	62-63	-2.2276577461056313
1101	64-65	-2.377129454093012
1101	66-67	-2.2066123090918524
1101	68-69	-2.0216727904044056
1101	70-71	-1.8526105244780524
1101	72-73	-1.5537761284654223
1101	74-75	-1.3921132594806807
1101	76-77	-1.5303307791952534
1101	78-79	-1.4478400239475882
1101	80-81	-1.971849905241438
1101	82-83	-1.7195419069275246
1101	84-85	-1.7578127984838403
1101	86-87	-1.989824425787976
1101	88-89	-2.4193331322444855
1101	90-91	-2.100193206608118
1101	92-93	-2.047088664458517
1101	94-95	-2.148959321636653
1101	96-97	-1.7629959456730262
1101	98-99	-1.9036798951933385
1101	100	-1.658791256413668
1102	1	0.10276185282834405
1102	2	-0.03603375570128975
1102	3	0.20205896173330018
1102	4	-0.20947498911054652
1102	5	0.2258051269460708
1102	6	0.1708634536735829
1102	7	0.24307664834704212
1102	8	0.4313873295488193
1102	9	0.18538763198669983
1102	10-11	0.01420181047602398
1102	12-13	0.2693796827839776
1102	14-15	0.45257345012716854
1102	16-17	0.43473388790766876
1102	18-19	0.3240291774160795
1102	20-21	0.324163666012808
1102	22-23	0.1379560264565285
1102	24-25	0.14078007727807318
1102	26-27	0.24716343930506923
1102	28-29	0.02472788420034533
1102	30-31	0.382547505420753
1102	32-33	0.019968490616520285
1102	34-35	0.32828536777140727
1102	36-37	0.5893403025874022
1102	38-39	0.011732181613005821
1102	40-41	-0.2479236497878432
1102	42-43	-0.451635420190712
1102	44-45	-0.4484664603779862
1102	46-47	0.6614213620190839
1102	48-49	0.629924677093129
1102	50-51	1.0326907126145954
1102	52-53	1.2690339017519747
1102	54-55	1.0606118090151995
1102	56-57	1.6559909440412817
1102	58-59	1.3094161275676974
1102	60-61	1.5413022175786821
1102	62-63	1.266528300405998
1102	64-65	1.8307719188405862
1102	66-67	2.279658354959704
1102	68-69	2.4030960524087064
1102	70-71	2.7200288703104043
1102	72-73	2.234189688289973
1102	74-75	2.2252865724050004
1102	76-77	2.0661248106058103
1102	78-79	2.6782457143544605
1102	80-81	2.5783041995498195
1102	82-83	2.541665714254883
1102	84-85	2.559643071507754
1102	86-87	3.0615905363863014
1102	88-89	2.307621196699195
1102	90-91	2.575906541220405
1102	92-93	2.408571184238596
1102	94-95	2.370163682005824
1102	96-97	2.500943533172588
1102	98-99	2.5437843805141434
1102	100	2.6680509963181898
1103	1	0.25856689116350395
1103	2	0.3766168467083517
1103	3	0.44329663971796407
1103	4	0.13170792436152112
1103	5	0.5061994314367624
1103	6	0.4698776924468575
1103	7	0.5307272507566836
1103	8	0.4439831674458645
1103	9	0.6599221336296353
1103	10-11	0.5735528510017645
1103	12-13	0.7960773826744543
1103	14-15	1.11043215768467
1103	16-17	1.03645897005444
1103	18-19	0.8486732080842074
1103	20-21	0.8982326692986788
1103	22-23	0.1643799037840239
1103	24-25	0.01803911342265252
1103	26-27	0.2751617963696873
1103	28-29	0.2307793628421848
1103	30-31	0.02637280662557373
1103	32-33	-0.30300522241742556
1103	34-35	0.39947923414599273
1103	36-37	0.4125193825435929
1103	38-39	0.5583504729602069
1103	40-41	0.30225433487809283
1103	42-43	0.1908892238399531
1103	44-45	0.12464416393746092
1103	46-47	0.3077110662907181
1103	48-49	-0.13595703155966632
1103	50-51	0.2045143708840378
1103	52-53	0.38951582946281604
1103	54-55	0.18006690211486998
1103	56-57	0.21910704480798415
1103	58-59	0.5231346379729551
1103	60-61	0.060606708268714726
1103	62-63	0.10175557313327133
1103	64-65	0.3804022583805633
1103	66-67	0.01035386426967122
1103	68-69	0.16199528570552957
1103	70-71	-0.4172246893829161
1103	72-73	-0.6477243314252519
1103	74-75	-0.5019176992269792
1103	76-77	-0.17620815763077147
1103	78-79	-0.5100757533344797
1103	80-81	-0.033689228708666974
1103	82-83	-0.07696791115585455
1103	84-85	-0.4877282318876439
1103	86-87	-0.46154747018543674
1103	88-89	-0.22113017241361987
1103	90-91	0.20953195195424712
1103	92-93	0.5603373397698128
1103	94-95	0.2821981836487595
1103	96-97	0.12786029111344277
1103	98-99	0.3850357496269581
1103	100	0.7682700543685748
1104	1	0.3309770391550444
1104	2	-0.2222203625939727
1104	3	0.2757490710076027
1104	4	0.33255359032347087
1104	5	0.2630704673775668
1104	6	0.3916535909669463
1104	7	0.12665748331482263
1104	8	0.3414461695600295
1104	9	0.2767297446232888
1104	10-11	0.17622198419414303
1104	12-13	0.2625150148097575
1104	14-15	0.554142517092707
1104	16-17	0.2867232406675413
1104	18-19	0.37985714043092855
1104	20-21	0.6405688215185563
1104	22-23	0.6355744069552713
1104	24-25	0.17356236923660617
1104	26-27	0.6434228957354406
1104	28-29	1.000071117599063
1104	30-31	0.25457048104418334
1104	32-33	-0.23878746343223156
1104	34-35	0.24990206712697471
1104	36-37	0.35636187725818047
1104	38-39	0.7775629465331448
1104	40-41	0.5061391128907786
1104	42-43	0.6684262217257881
1104	44-45	0.6045595973412645
1104	46-47	0.1558875990602715
1104	48-49	1.035290325734202
1104	50-51	1.237019656296301
1104	52-53	0.45346931783490874
1104	54-55	0.8083702847787215
1104	56-57	0.7447412942794429
1104	58-59	0.8859781897699968
1104	60-61	0.9229217188395396
1104	62-63	0.9932724864525078
1104	64-65	1.2100798482325708
1104	66-67	1.2585039699779124
1104	68-69	1.7166993026188493
1104	70-71	2.478784824359156
1104	72-73	2.3415727087438825
1104	74-75	2.462537903310018
1104	76-77	2.7371110812698625
1104	78-79	2.4940204411686935
1104	80-81	2.4351268389446084
1104	82-83	2.4083650698166608
1104	84-85	2.224745341051044
1104	86-87	2.2659895277003983
1104	88-89	2.2725273328717925
1104	90-91	2.539341677112809
1104	92-93	2.2959345913554365
1104	94-95	2.130110445805208
1104	96-97	2.202120333396742
1104	98-99	2.1021340582950323
1104	100	2.399348278470054
1105	1	-0.2341903515937389
1105	2	0.16507838516989182
1105	3	-0.20930076287943677
1105	4	0.1601794528330558
1105	5	0.048656973894303235
1105	6	-0.22143099886183393
1105	7	-0.5118551668257325
1105	8	-0.23483801137531657
1105	9	0.027304751012252382
1105	10-11	0.04889001383892122
1105	12-13	-0.12337816178109051
1105	14-15	-0.07350890125638898
1105	16-17	-0.23314642455095935
1105	18-19	-0.6048732454622439
1105	20-21	-0.4920020959360869
1105	22-23	-0.6299507655466527
1105	24-25	-0.5018784689949314
1105	26-27	-0.4446808609729658
1105	28-29	-0.42019466313183784
1105	30-31	-0.8355777428249738
1105	32-33	-0.5533798477920584
1105	34-35	-0.3362850016182364
1105	36-37	-0.2743562418320309
1105	38-39	0.8025437796535115
1105	40-41	0.6505934957172528
1105	42-43	0.3820730400237764
1105	44-45	0.08992887922217108
1105	46-47	-0.7023413812617321
1105	48-49	-0.7056373512399858
1105	50-51	-1.3168392754696079
1105	52-53	-1.2643303243833373
1105	54-55	-1.1171983326503643
1105	56-57	-1.2540448033438665
1105	58-59	-1.0324347925964759
1105	60-61	-1.2057519330899247
1105	62-63	-1.4862189523412574
1105	64-65	-1.3451846547063475
1105	66-67	-1.13262815371235
1105	68-69	-1.0611565624463175
1105	70-71	-1.0417876264458528
1105	72-73	-0.8413432125441318
1105	74-75	-0.9075745423838235
1105	76-77	-1.2758585072811215
1105	78-79	-1.738409919168646
1105	80-81	-1.472999918019358
1105	82-83	-1.1660663127542534
1105	84-85	-1.275190769350182
1105	86-87	-1.1742098078477774
1105	88-89	-1.0720292733127188
1105	90-91	-1.0162422738199766
1105	92-93	-0.8917730498405767
1105	94-95	-0.8098972209466453
1105	96-97	-0.933453395200246
1105	98-99	-0.9831585560673481
1105	100	-0.8296320435335218
1106	1	0.5100990668529803
1106	2	0.6378528017645309
1106	3	0.6703140043757756
1106	4	0.25377125429002234
1106	5	0.36523926800468587
1106	6	0.37283989877985135
1106	7	0.34392949794769834
1106	8	0.21262463833452472
1106	9	0.5416892429248321
1106	10-11	0.4677688877739712
1106	12-13	0.5201580772607599
1106	14-15	0.9297631076336046
1106	16-17	0.6823987044773219
1106	18-19	0.6678764767256808
1106	20-21	0.6996882362036843
1106	22-23	0.7232409865215104
1106	24-25	0.8726739448833243
1106	26-27	0.8051030629682643
1106	28-29	0.8815709869484181
1106	30-31	0.8655581998839992
1106	32-33	0.9243951861627551
1106	34-35	0.7779266294473288
1106	36-37	1.1840336828500284
1106	38-39	1.0479444464025036
1106	40-41	1.293635846624774
1106	42-43	1.4096073035130914
1106	44-45	1.4349097410569698
1106	46-47	2.3585920673121663
1106	48-49	2.2391706497477912
1106	50-51	2.259034289168003
1106	52-53	2.0973809980021407
1106	54-55	2.730437177906495
1106	56-57	2.6982949916925634
1106	58-59	2.485468652273262
1106	60-61	2.2057803548468584
1106	62-63	2.0811350419790315
1106	64-65	1.9514569059801552
1106	66-67	1.6039442626353484
1106	68-69	2.1552281764103327
1106	70-71	1.49537745566305
1106	72-73	1.2900310311896632
1106	74-75	0.964693638873122
1106	76-77	1.3010645696419552
1106	78-79	0.9739002425797167
1106	80-81	1.1780063790543558
1106	82-83	1.3627026710709096
1106	84-85	1.131526109276809
1106	86-87	1.2936874634202802
1106	88-89	1.3015971003136535
1106	90-91	1.6344553431697761
1106	92-93	1.7302173193408024
1106	94-95	1.512982146876542
1106	96-97	1.6481616189990405
1106	98-99	1.8925051061131697
1106	100	1.7892097887914566
1107	1	-0.07351607441461283
1107	2	-0.40250936688387995
1107	3	-0.3521579057365827
1107	4	0.07301419091051287
1107	5	-0.22372113343526223
1107	6	0.10535871627740079
1107	7	0.30378550318386743
1107	8	0.32880223187657975
1107	9	0.13719486090236188
1107	10-11	-0.07908395394087364
1107	12-13	-0.10054662438645323
1107	14-15	-0.35746281142388625
1107	16-17	-0.20791437504706778
1107	18-19	0.16553110746637856
1107	20-21	-0.04892090528208115
1107	22-23	0.08262791790582469
1107	24-25	0.14364590953915268
1107	26-27	-0.050356738493505304
1107	28-29	0.11239895684042267
1107	30-31	0.40804756390712527
1107	32-33	0.6006796849082576
1107	34-35	1.0861517848962166
1107	36-37	0.15010764379690045
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2216	68-69	0.4168654155756606
2216	70-71	0.6915842648207757
2216	72-73	0.7034803849110425
2216	74-75	0.7445365044640653
2216	76-77	0.8273803591156401
2216	78-79	0.6239382589690052
2216	80-81	0.897445426110199
2216	82-83	0.7116086438680682
2216	84-85	0.7537276806209015
2216	86-87	0.19421124478380491
2216	88-89	0.12114597249410153
2216	90-91	0.001397980662382281
2216	92-93	0.15333973211430774
2216	94-95	0.40467767783878017
2216	96-97	0.23918975112711394
2216	98-99	0.20770321374178735
2216	100	0.40634250823050166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	7.0
6	33.0
7	62.0
8	104.0
9	161.0
10	298.0
11	470.0
12	819.0
13	1173.0
14	1199.0
15	1662.0
16	2270.0
17	3384.0
18	3772.0
19	1463.0
20	539.0
21	448.0
22	457.0
23	567.0
24	738.0
25	1086.0
26	1200.0
27	1353.0
28	636.0
29	685.0
30	716.0
31	889.0
32	1133.0
33	1394.0
34	1890.0
35	3171.0
36	5670.0
37	11062.0
38	12893.0
39	3346.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.97320585634862	18.980683640287126	14.402601489562572	18.643509013801683
2	16.632391131392886	21.922332137442393	39.86519957368239	21.580077157482325
3	19.696220734283017	29.621474258152457	33.796192273700925	16.886112733863598
4	11.659501804946002	20.938871496831982	34.599541648567275	32.802085049654735
5	9.438145024789922	38.85951378799	34.23106304579021	17.471278141429877
6	24.58021899012897	39.298392774224475	15.378739084195864	20.7426491514507
7	19.336139362801635	39.425712616647445	20.502988271595694	20.73515974895523
8	25.160275614140204	30.78490113840623	20.573696824445776	23.48112642300779
9	24.505324965174278	13.991701742035021	21.624900765416935	39.87807252737377
10-11	23.04489147855784	26.612093887149683	25.679663276463806	24.663351357828674
12-13	28.625245277931725	24.92847620616827	28.139931996225343	18.30634651967466
14-15	24.46937583319603	23.137760069501656	31.670436332589386	20.72242776471293
16-17	17.178442503857042	27.442668623897188	32.593130720031155	22.785758152214616
18-19	17.339464657509623	20.598852623537695	42.16608498973952	19.89559772921316
20-21	18.6583484369617	18.52279025179371	43.61303755186412	19.20582375938048
22-23	17.153727475621995	20.36068962418178	44.28109225445994	18.204490645736282
24-25	18.563981965518792	21.52753853297584	43.662467608334204	16.246011893171165
26-27	18.042719551834153	21.11562139572505	44.85328260511376	15.988376447327033
28-29	17.182936145354326	20.752385374694807	44.94090861431075	17.12376986564012
30-31	19.085244379203427	19.994457842153356	44.85103578436512	16.069261994278097
32-33	17.89742514342206	20.46254549812016	45.90779047647578	15.732238881981994
34-35	17.57463189586735	18.501070984556854	45.306391456089635	18.617905663486166
36-37	16.539596470993544	20.8624795913782	44.836056979374185	17.76186695825407
38-39	17.2608259313072	17.87046329443837	45.94823324995132	18.920477524303113
40-41	17.46828238043169	18.279384670690973	45.29291053159779	18.95942241727955
42-43	18.498075223558665	18.30634651967466	43.60704602986773	19.588532226898938
44-45	17.877203756684292	19.387067299770823	42.46641002980782	20.26931891373706
46-47	20.55915879031171	19.394556702266293	38.44310300924192	21.603181498180074
48-49	20.60933778703135	19.65069426761133	35.98957475172631	23.750393193631012
50-51	19.443986758736386	20.32773625320172	36.18729497760669	24.040982010455206
52-53	19.628226060124923	21.341052410838664	36.109405191653806	22.921316337382603
54-55	18.688306046943577	21.896766076002454	33.95395515345786	25.460972723596107
56-57	20.35469810218541	22.323662018244185	31.32592381779782	25.99571606177259
58-59	18.736238222914576	23.211156213957253	33.5682509249412	24.484354638186964
60-61	19.14815536016537	25.319423016431745	30.897529995056992	24.63489162834589
62-63	17.38664789323108	26.75738829556178	30.969428259013497	24.886535552193646
64-65	17.349200880753735	27.500337023112298	30.951453693024373	24.1990084031096
66-67	17.447312053444374	26.70196671709531	30.135857761267804	25.714863468192505
68-69	17.785217306640902	26.090277933808164	30.816126302229613	25.308378457321318
70-71	18.844834559098874	25.379338236395498	30.34705891164003	25.428768292865595
72-73	19.071157346035456	24.310033627669057	31.195841852592476	25.422967173703015
74-75	19.02397375693711	24.556436815182632	30.330809385789504	26.088780042090757
76-77	19.74955438055152	23.887449259298094	30.111891673282305	26.251104686868082
78-79	20.085828552598073	24.22746813259238	30.168811132247868	25.517892182561674
80-81	19.856652836236723	24.330072946780305	29.77711538173485	26.036158835248123
82-83	19.641854090367808	24.454580178398906	29.99977531624239	25.9037904149909
84-85	19.624481358877187	25.232545947484308	28.9937238807088	26.149248812929706
86-87	19.773668561499687	25.722545517184564	28.551314025509093	25.95247189580665
88-89	19.446067196932248	25.801764556089818	27.961772944471907	26.79039530250603
90-91	20.240716607498616	25.272996899294476	27.579801974265646	26.906484518941266
92-93	20.435140802875974	25.77216896345117	27.45880766926303	26.333882564409826
94-95	20.14230077890953	25.873277411623725	27.453565008987418	26.53085680047933
96-97	19.796136937260805	25.5555305906936	27.379213756637533	27.269118715408062
98-99	20.104702631046802	25.697081357988633	26.66771519086885	27.530500820095718
100	20.10305417833765	25.1029792843127	26.738664789323106	28.05530174802654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	35.0
2	80.0
3	139.0
4	86.5
5	44.5
6	95.0
7	169.0
8	251.5
9	291.0
10	205.0
11	105.0
12	86.0
13	84.5
14	89.0
15	88.0
16	93.0
17	96.5
18	103.0
19	127.0
20	143.0
21	174.0
22	235.0
23	310.5
24	375.5
25	452.5
26	592.5
27	795.0
28	1029.0
29	1202.5
30	1365.5
31	1578.0
32	1719.5
33	1839.0
34	1902.5
35	1897.0
36	1976.5
37	1943.5
38	1762.5
39	1671.0
40	1617.0
41	1598.0
42	1640.0
43	1671.0
44	1697.0
45	1685.0
46	1867.5
47	2437.0
48	3300.0
49	3592.5
50	3178.5
51	2667.5
52	1963.0
53	1538.0
54	1366.0
55	1237.0
56	1178.5
57	1094.0
58	1006.0
59	807.0
60	732.5
61	686.0
62	534.0
63	433.0
64	371.0
65	338.5
66	267.5
67	185.5
68	169.0
69	161.0
70	112.0
71	83.0
72	65.5
73	49.0
74	45.0
75	31.5
76	24.5
77	16.5
78	11.0
79	11.0
80	9.0
81	6.5
82	9.5
83	9.0
84	2.5
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04493641497281347
2	0.21569479186950463
3	0.002995760998187565
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0014978804990937824
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	7.489402495468912E-4
70-71	0.0
72-73	7.489402495468912E-4
74-75	7.489402495468912E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	7.489402495468912E-4
84-85	0.0
86-87	7.489402495468912E-4
88-89	0.002995760998187565
90-91	0.002995760998187565
92-93	0.0014978804990937824
94-95	0.0014978804990937824
96-97	7.489402495468912E-4
98-99	7.489402495468912E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	66761.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.291008223363946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.27126897737038	43.54338610865625
2	8.275565740475509	8.654753523763874
3	2.8186765969636207	4.421743233324845
4	1.6069894013176738	3.3612438399664475
5	0.9109137782870237	2.381629993559114
6	0.6531079919793755	2.0491005227602943
7	0.4096247493554856	1.4993783795928761
8	0.28358636493841305	1.1863213552822756
9	0.2148381552563735	1.011069336888303
>10	1.3577771412202808	12.558230104402272
>50	0.10025780578630766	3.6009047198214525
>100	0.08307075336579776	7.670646035859259
>500	0.005729017473503294	1.9772022588037927
>1k	0.008593526210254943	6.084390587318944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1533	2.2962508051107684	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1281	1.918784919339135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1248	1.8693548628690402	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	708	1.060499393358398	RNA PCR Primer, Index 33 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	612	0.9167028654453948	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT	454	0.6800377465885772	RNA PCR Primer, Index 33 (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	439	0.6575695391021704	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.5661988286574497	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	281	0.4209044202453528	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.36098920028160153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	215	0.3220443073051632	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	211	0.31605278530878805	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	207	0.31006126331241296	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	202	0.30257186081694404	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	176	0.26362696784050565	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	170	0.25463968484594296	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	147	0.220188433366786	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	139	0.20820538937403574	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT	135	0.20221386737766062	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	130	0.1947244648821917	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	129	0.19322658438309792	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	125	0.1872350623867228	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	125	0.1872350623867228	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	121	0.18124354039034768	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	117	0.17525201839397253	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	115	0.17225625739578498	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG	114	0.17075837689669118	RNA PCR Primer, Index 15 (95% over 24bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	111	0.16626473539940984	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	111	0.16626473539940984	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.16476685490031606	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	106	0.15877533290394094	No Hit
GTACATGGGGGAGGCCCGAACCCACGTACGTTAAAAAGTGCGGGGATGAG	105	0.15727745240484714	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	105	0.15727745240484714	No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	102	0.1527838109075658	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	92	0.13780500591662798	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	88	0.13181348392025283	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	86	0.12881772292206528	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	84	0.1258219619238777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	82	0.12282620092569013	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	81	0.12132832042659636	No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	80	0.11983043992750259	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	78	0.11683467892931501	No Hit
CTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCTTTTGACTACGGAT	77	0.11533679843022124	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	77	0.11533679843022124	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	76	0.11383891793112745	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76	0.11383891793112745	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	76	0.11383891793112745	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT	74	0.11084315693293989	RNA PCR Primer, Index 33 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	73	0.1093452764338461	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT	72	0.10784739593475232	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	70	0.10485163493656476	No Hit
GTACAGGGCGCGTCTGTAGTCCCAGCTGCTTGAGAGGCTGAGCAGGAGAA	68	0.1018558739383772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0014978804990937824	0.0
14-15	0.0	0.0	0.0	0.0014978804990937824	0.0
16-17	0.0	0.0	0.0	0.002246820748640674	0.0
18-19	0.0	0.0	0.0	0.002995760998187565	0.0
20-21	0.0	0.0	0.0	0.002995760998187565	0.0
22-23	0.0	0.0	0.0	0.008987282994562694	0.0
24-25	0.0	0.0	0.0	0.02696184898368808	0.0
26-27	0.0	0.0	0.0	0.0591662797142044	0.0
28-29	0.0	0.0	0.0	0.14005182666526866	0.0
30-31	0.0	0.0	0.0	0.3123080840610536	0.0
32-33	0.0	0.0	0.0	0.5647009481583559	0.0
34-35	0.0	0.0	0.0	0.9369242521831609	0.0
36-37	0.0	0.0	0.0	1.4559398451191563	0.0
38-39	0.0	0.0	0.0	2.1389733527059214	0.0
40-41	0.0	0.0	0.0	2.777819385569419	0.0
42-43	0.0	0.0	0.0	3.2489028025344138	0.0
44-45	0.0	0.0	0.0	3.639100672548344	0.0
46-47	0.0	0.0	0.0	4.105690448016057	0.0
48-49	0.0	0.0	0.0	4.562544000239661	0.0
50-51	0.0	0.0	0.0	5.014903910965983	0.0
52-53	0.0	0.0	0.0	5.380386752744866	0.0
54-55	0.0	0.0	0.0	5.801291172990219	0.0
56-57	0.0	0.0	0.0	6.318808885427121	0.0
58-59	0.0	0.0	0.0	6.726981321430177	0.0
60-61	0.0	0.0	0.0	7.148634681925076	0.0
62-63	0.0	0.0	0.0	7.541828312937194	0.0
64-65	0.0	0.0	0.0	7.926783601204296	0.0
66-67	0.0	0.0	0.0	8.391875496172915	0.0
68-69	0.0	0.0	0.0	8.943095519839428	0.0
70-71	0.0	0.0	0.0	9.528017854735548	0.0
72-73	0.0	0.0	0.0	10.112191249382125	0.0
74-75	0.0	0.0	0.0	10.669402795045011	0.0
76-77	0.0	0.0	0.0	11.210137655217867	0.0
78-79	0.0	0.0	0.0	11.857970971075927	0.0
80-81	0.0	0.0	0.0	12.520034151675379	0.0
82-83	0.0	0.0	0.0	13.162624885786613	0.0
84-85	0.0	0.0	0.0	13.84041581162655	0.0
86-87	0.0	0.0	0.0	14.54367070595108	0.0
88	0.0	0.0	0.0	15.038720210901575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCTCG	20	0.0021541764	70.49887	6
GAACATT	20	0.0021541764	70.49887	6
ATTGAAC	20	0.0021541764	70.49887	3
TATTGAA	25	0.005199391	56.441383	2
TTAATAG	25	0.0052148504	56.3991	5
ACTTGTT	25	0.0052148504	56.3991	7
ACATTCT	45	0.0010071778	41.777115	8
CTACTGA	25	0.0016967775	37.5994	46-47
GCTAGTC	25	0.0016967775	37.5994	74-75
GGTATCA	1350	0.0	29.614305	1
ATGGGGA	65	0.0061137723	28.922617	5
CTCACTC	65	0.0061137723	28.922617	3
CGCATCG	45	9.474412E-4	26.110695	16-17
GTATCAA	1845	0.0	24.983086	1
GTGTTTA	50	0.0017507457	23.499626	48-49
ATGGGGG	100	0.0017416173	23.499626	5
GAGTACT	1565	0.0	22.073132	12-13
TATCAAC	2145	0.0	21.488947	2
CAACGCA	2170	0.0	21.225468	5
ATCAACG	2175	0.0	21.176674	3
>>END_MODULE