FastQCFastQC Report
Wed 25 May 2016
SRR1033099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118272
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29192.468039772727273No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17501.4796401515151516No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11760.9943181818181818No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8550.7229099025974026No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5390.4557291666666667No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5340.4515016233766234No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5150.4354369588744589No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4810.4066896645021645No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4630.3914705086580087No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4580.3872429653679654No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4350.36779626623376627No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3800.32129329004329005No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3720.3145292207792208No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3660.30945616883116883No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3170.2680262445887446No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT3060.25872564935064934No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2970.25111607142857145No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2940.24857954545454544No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.22236877705627706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.2012310606060606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.18347537878787878No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC2140.18093885281385283No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2060.17417478354978355No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG2010.16994724025974026No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.16571969696969696No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1930.163183170995671No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.1530370670995671No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1460.12344426406926408No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.10399756493506493No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAAT250.005200274856.46613
TGCTATA250.005200274856.46612
CGCAGAA1450.055.168032
TACATGA708.442839E-847.0550844
GAGAACA2500.043.309031
GGAAACA2200.042.7954831
GTAACAA450.001002356641.844471
TAACAAC1600.041.190651
TGGACAG608.71567E-539.212575
CCGTATC500.00170375137.5644894
GCAGAAA1800.036.5983963
TGTGTAC550.002691682434.221888
AGAACAA2400.033.3306852
ACAACAC4550.033.0936855
CACAAAG1151.102926E-732.7617349
ACATGGA1308.938514E-932.5765953
GGGTATC300.004130598631.37005622-23
ATCAACC600.004122305731.3700568
ACAGCAG600.004122305731.3700568
CGCCGTA902.5911311E-531.30373294
Produced by FastQC (version 0.11.5)