##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033099_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	118272
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.727822307900432	31.0	26.0	34.0	12.0	34.0
2	27.6835091991342	31.0	27.0	34.0	7.0	34.0
3	27.92167207792208	31.0	27.0	34.0	10.0	34.0
4	30.66329308712121	37.0	31.0	37.0	7.0	37.0
5	31.378491950757574	37.0	31.0	37.0	10.0	37.0
6	31.104099025974026	37.0	31.0	37.0	8.0	37.0
7	31.470305735930737	37.0	32.0	37.0	8.0	37.0
8	30.88655810335498	37.0	31.0	37.0	8.0	37.0
9	31.851283482142858	39.0	31.0	39.0	8.0	39.0
10-11	32.221844561688314	39.0	31.0	39.0	7.5	39.0
12-13	32.298616747835496	39.0	31.0	39.0	7.5	39.0
14-15	33.39442978896104	40.0	32.0	41.0	7.0	41.0
16-17	33.68318790584415	40.0	32.0	41.0	8.0	41.0
18-19	33.73655641233766	40.0	32.0	41.0	8.0	41.0
20-21	33.757199506222946	40.0	32.0	41.0	8.0	41.0
22-23	33.69049310064935	40.0	31.0	41.0	8.0	41.0
24-25	33.54258404356061	40.0	31.0	41.0	5.0	41.0
26-27	32.95416920319264	39.0	30.5	41.0	2.0	41.0
28-29	32.55214251893939	39.0	30.0	41.0	2.0	41.0
30-31	32.27644751082251	40.0	30.0	41.0	2.0	41.0
32-33	31.859548329274894	40.0	30.0	41.0	2.0	41.0
34-35	31.491654829545453	39.5	30.0	41.0	2.0	41.0
36-37	31.139043898809526	39.0	30.0	41.0	2.0	41.0
38-39	30.799001454274894	39.0	28.5	41.0	2.0	41.0
40-41	30.614798938041126	39.0	27.5	41.0	2.0	41.0
42-43	30.354944534632033	39.0	27.0	41.0	2.0	41.0
44-45	30.163589015151516	39.0	26.5	41.0	2.0	41.0
46-47	29.919528713474023	38.5	25.0	41.0	2.0	41.0
48-49	29.67758641098485	38.0	25.0	41.0	2.0	41.0
50-51	29.48248528814935	38.0	24.5	41.0	2.0	41.0
52-53	29.34182224025974	38.0	23.5	41.0	2.0	41.0
54-55	29.167279660443725	38.0	23.0	41.0	2.0	41.0
56-57	28.949983935335496	37.5	22.5	40.5	2.0	41.0
58-59	28.725391470508658	37.0	20.0	40.0	2.0	41.0
60-61	28.449768330627705	36.0	16.5	40.0	2.0	41.0
62-63	28.230777360660174	36.0	11.5	40.0	2.0	41.0
64-65	27.963211918290042	35.5	7.5	39.5	2.0	41.0
66-67	27.624370096049784	35.0	2.0	39.0	2.0	41.0
68-69	27.27938565340909	35.0	2.0	39.0	2.0	41.0
70-71	26.9004751758658	35.0	2.0	37.5	2.0	40.0
72-73	26.563366646374458	35.0	2.0	37.0	2.0	40.0
74-75	26.288559422348484	35.0	2.0	37.0	2.0	39.0
76-77	25.98903375270563	34.0	2.0	36.0	2.0	39.0
78-79	25.635192606872295	34.0	2.0	36.0	2.0	38.5
80-81	25.32339860660173	34.0	2.0	35.0	2.0	37.0
82-83	25.13492627164502	34.0	2.0	35.0	2.0	37.0
84-85	24.99126166801948	34.0	2.0	35.0	2.0	36.5
86-87	24.914768499729437	34.0	2.0	35.0	2.0	36.0
88-89	24.719231939935064	34.0	2.0	35.0	2.0	36.0
90-91	24.49798346185065	34.0	2.0	35.0	2.0	36.0
92-93	24.263731060606062	34.0	2.0	35.0	2.0	35.0
94-95	24.052172111742426	34.0	2.0	35.0	2.0	35.0
96-97	23.857971455627705	33.5	2.0	35.0	2.0	35.0
98-99	23.67773860254329	33.0	2.0	35.0	2.0	35.0
100	23.54300257034632	33.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17717139670307347
1101	2	0.05414944325955062
1101	3	0.2183985114602045
1101	4	-0.16500886744060494
1101	5	0.3901101843221255
1101	6	0.44284674495302667
1101	7	-0.7993390838667054
1101	8	0.2157029902902572
1101	9	0.2111316161435397
1101	10-11	0.920271240829571
1101	12-13	0.5597261138416556
1101	14-15	0.3606645677145437
1101	16-17	0.09833128211106157
1101	18-19	0.4160130582594235
1101	20-21	0.12269550393136797
1101	22-23	0.6245922098419427
1101	24-25	0.6813480784626904
1101	26-27	1.2482448141631934
1101	28-29	1.5270608503605558
1101	30-31	1.788937306768318
1101	32-33	2.0090278579891425
1101	34-35	1.8148883395125672
1101	36-37	1.8342786851872503
1101	38-39	2.0037141685912054
1101	40-41	1.5749341341063499
1101	42-43	1.75805116571523
1101	44-45	1.755909115917163
1101	46-47	1.9259091153158714
1101	48-49	2.0511699088144475
1101	50-51	1.7464919693447527
1101	52-53	2.048658820771724
1101	54-55	1.84144348394166
1101	56-57	2.0558869656723893
1101	58-59	2.1326061473462197
1101	60-61	2.120442798889435
1101	62-63	2.264095079342507
1101	64-65	2.0201898722850373
1101	66-67	1.7517704649921733
1101	68-69	1.7865348864473738
1101	70-71	1.8425211395920975
1101	72-73	1.7645111382262684
1101	74-75	1.6020272068419814
1101	76-77	1.5837318794030821
1101	78-79	1.773770556012412
1101	80-81	1.7630393516231955
1101	82-83	1.182601548042161
1101	84-85	1.1023257069648444
1101	86-87	0.9723163886178767
1101	88-89	0.9664345341892648
1101	90-91	1.3190573531215328
1101	92-93	1.2278295121569833
1101	94-95	1.3283359874471046
1101	96-97	0.8780030002135106
1101	98-99	1.012552771444728
1101	100	0.730773753110455
1102	1	-0.6949374468343414
1102	2	0.025918150742544555
1102	3	0.865677423024831
1102	4	0.15165779922606148
1102	5	0.4649401162949154
1102	6	0.454411370803367
1102	7	-0.03879486617963224
1102	8	0.29529482702495
1102	9	0.040383316823813686
1102	10-11	0.2971419891288818
1102	12-13	0.17588257642668026
1102	14-15	0.39280742485739495
1102	16-17	-0.12190681312703333
1102	18-19	-0.22242231589022765
1102	20-21	-0.23070585661284326
1102	22-23	0.141428944535825
1102	24-25	0.5852596430885413
1102	26-27	0.3882108005577507
1102	28-29	0.6935574490000107
1102	30-31	0.9552638373805706
1102	32-33	1.0287557491456063
1102	34-35	1.580024393934334
1102	36-37	1.705707256615824
1102	38-39	1.4982719917204612
1102	40-41	1.3892198483920666
1102	42-43	1.0369627303410809
1102	44-45	0.9365213608151208
1102	46-47	0.9378138772206341
1102	48-49	1.0202175278620658
1102	50-51	0.7901994523379514
1102	52-53	0.7319921541050576
1102	54-55	0.5584502866627474
1102	56-57	0.7363291425431377
1102	58-59	0.7803952629924744
1102	60-61	0.8258849757601823
1102	62-63	0.6894352153969265
1102	64-65	0.4189993960945593
1102	66-67	0.7427568595499991
1102	68-69	0.7377253626378497
1102	70-71	0.8022150171431193
1102	72-73	0.9424022946888542
1102	74-75	1.078217683032456
1102	76-77	1.0568611311037586
1102	78-79	1.0603351818627509
1102	80-81	0.7191618006027873
1102	82-83	1.0475675344367161
1102	84-85	0.96133931240702
1102	86-87	0.7116021029035906
1102	88-89	0.48837330969947246
1102	90-91	0.704771638835819
1102	92-93	0.763713865898481
1102	94-95	1.010648912617171
1102	96-97	1.0368465376284774
1102	98-99	1.2412942680433687
1102	100	0.7953996034505906
1103	1	1.3110649541260422
1103	2	1.0504613013361173
1103	3	0.6655973910120281
1103	4	1.074160133493102
1103	5	0.7215761040233417
1103	6	1.0237057552238014
1103	7	0.8098112429306781
1103	8	0.6761138212893236
1103	9	1.0399831567597886
1103	10-11	1.0726521932105229
1103	12-13	0.8827653295279205
1103	14-15	0.8942079850814935
1103	16-17	0.8953667629700703
1103	18-19	0.6083099770269342
1103	20-21	0.876803813922038
1103	22-23	1.1392280641836834
1103	24-25	1.5010259496111544
1103	26-27	1.7141411727066114
1103	28-29	1.5174203274847429
1103	30-31	1.7915983711940981
1103	32-33	1.8822304723682244
1103	34-35	1.7865736803155592
1103	36-37	1.4461367617512124
1103	38-39	1.651800069618286
1103	40-41	1.761768867999912
1103	42-43	1.1376029864435253
1103	44-45	1.3863012727799067
1103	46-47	1.3593825046716148
1103	48-49	1.0617674811767266
1103	50-51	1.2665900085604385
1103	52-53	1.2305915938809662
1103	54-55	1.4251969853422253
1103	56-57	1.4553500842531548
1103	58-59	1.4682037197084945
1103	60-61	1.3656342087867301
1103	62-63	1.3885815405936732
1103	64-65	1.3583084530506753
1103	66-67	1.5279375985122456
1103	68-69	1.2718056614240325
1103	70-71	1.2505276755398107
1103	72-73	1.4227277582075928
1103	74-75	1.5884884580091132
1103	76-77	1.539520861662652
1103	78-79	1.3471832544251114
1103	80-81	1.2776518632945333
1103	82-83	1.3636939850169476
1103	84-85	1.391377994546545
1103	86-87	1.507120310186501
1103	88-89	1.464497092545944
1103	90-91	1.6641553923372179
1103	92-93	1.7190293254156224
1103	94-95	1.5280558754022877
1103	96-97	1.5512522999333989
1103	98-99	1.3754145828359512
1103	100	1.4259184776669436
1104	1	0.3563921759238511
1104	2	0.14960398871409808
1104	3	0.4930738361355296
1104	4	-0.372151724583464
1104	5	-0.1163833221713837
1104	6	0.503885705991987
1104	7	-0.1331053176329391
1104	8	0.16635234093960705
1104	9	0.35269005770198447
1104	10-11	0.5001413706996942
1104	12-13	0.13212871124424197
1104	14-15	0.24670352875349977
1104	16-17	0.3791754379552188
1104	18-19	0.7199091621555311
1104	20-21	0.8019162831521527
1104	22-23	1.1112805215302544
1104	24-25	0.984919507034121
1104	26-27	1.1453227362411198
1104	28-29	0.6046582529579538
1104	30-31	0.34672951456053624
1104	32-33	0.1375992865605724
1104	34-35	-0.6140077643835333
1104	36-37	-0.4566304057218389
1104	38-39	-0.5131689482919057
1104	40-41	-0.8542866451144278
1104	42-43	-0.4757150680510023
1104	44-45	-0.4187662087581643
1104	46-47	-0.3630519236451626
1104	48-49	-0.5598690522245136
1104	50-51	-0.5012353033825221
1104	52-53	-0.6467957246828249
1104	54-55	-0.8286863861882097
1104	56-57	-0.8139182291328062
1104	58-59	-0.7053808656407945
1104	60-61	-0.977609149162511
1104	62-63	-0.8427231024756772
1104	64-65	-0.728186751091588
1104	66-67	-0.8173853791636709
1104	68-69	-0.6572962823837969
1104	70-71	-0.5168944448234853
1104	72-73	-0.7952291215139908
1104	74-75	-0.44342733861256534
1104	76-77	-0.39613825046704676
1104	78-79	-0.3122684050265505
1104	80-81	-0.8921554535716112
1104	82-83	-0.4875283220877087
1104	84-85	-0.3908561112169764
1104	86-87	-0.8306056893042033
1104	88-89	-0.9527213099665737
1104	90-91	-1.1588647248005444
1104	92-93	-1.515352306024834
1104	94-95	-1.4697159606048409
1104	96-97	-1.2862827140722004
1104	98-99	-1.0053043714124144
1104	100	-1.139356117019414
1105	1	-0.5183588399314587
1105	2	-0.33800656024614284
1105	3	-0.19750858757572587
1105	4	-0.7805653968008173
1105	5	0.2157017706499076
1105	6	-0.29852047678229354
1105	7	-1.3625292679157859
1105	8	-0.42224617710676426
1105	9	-0.6497798650133397
1105	10-11	-0.21978134462179355
1105	12-13	-0.5036174444405574
1105	14-15	-0.33988319740377904
1105	16-17	-0.44472305618867836
1105	18-19	-0.6011210346415368
1105	20-21	-0.6436059859897512
1105	22-23	-1.0297241792903904
1105	24-25	-1.0223109925276646
1105	26-27	-0.9145728896054273
1105	28-29	-0.4528471250995665
1105	30-31	-0.41145708411686854
1105	32-33	-1.10999054691883
1105	34-35	-1.1238276990500964
1105	36-37	-0.8070008941291356
1105	38-39	-0.8209133511283362
1105	40-41	-0.7641543847367664
1105	42-43	-0.9054019455906399
1105	44-45	-0.8180172644509369
1105	46-47	-0.9292617874010425
1105	48-49	-0.918724920282834
1105	50-51	-0.4631706073423629
1105	52-53	-0.8257057716910303
1105	54-55	-0.6268299604054022
1105	56-57	-0.5579824471234041
1105	58-59	-0.6871353075179378
1105	60-61	-0.6134748172367708
1105	62-63	-0.5421494430063127
1105	64-65	-0.6122597333240769
1105	66-67	-0.4401445218614626
1105	68-69	-0.47860534142291655
1105	70-71	-0.6809451180766111
1105	72-73	-0.952447669836836
1105	74-75	-0.8142742830791434
1105	76-77	-0.6317370075381987
1105	78-79	-0.8709489882470081
1105	80-81	-0.6886258543364896
1105	82-83	-0.905522904192722
1105	84-85	-0.7996681580658311
1105	86-87	-1.0885731819342723
1105	88-89	-0.9619835201490332
1105	90-91	-0.6975070640563779
1105	92-93	-0.47696891027947785
1105	94-95	-0.7238988942356279
1105	96-97	-0.39539752564100183
1105	98-99	-0.4178810585289803
1105	100	-0.3318905764250424
1106	1	-0.13457839196979293
1106	2	-0.10168318564999979
1106	3	-0.7077637877790153
1106	4	-0.8472151227237852
1106	5	-0.2650039323304547
1106	6	-0.14100786197850468
1106	7	0.044447898381815065
1106	8	-0.040171904046175655
1106	9	-0.12859788512442094
1106	10-11	-0.2596949468289296
1106	12-13	-0.3720786198861603
1106	14-15	-0.1439634965289045
1106	16-17	-0.4531268751163253
1106	18-19	-0.24387705163067608
1106	20-21	0.37370987417651236
1106	22-23	0.17258460206510762
1106	24-25	0.15672846730462453
1106	26-27	0.3587055073161949
1106	28-29	0.5408182468102609
1106	30-31	0.9090133845367063
1106	32-33	0.8451225325453571
1106	34-35	0.6976229126317541
1106	36-37	1.059046225339408
1106	38-39	1.3678730865962798
1106	40-41	1.4010541679186943
1106	42-43	1.041652180085478
1106	44-45	1.2106428437278112
1106	46-47	1.2021136799819772
1106	48-49	1.2912797989243359
1106	50-51	1.0836221468595468
1106	52-53	1.180991872335543
1106	54-55	0.8389920891825753
1106	56-57	0.4318168050637645
1106	58-59	0.21842609662770585
1106	60-61	0.4824884455504659
1106	62-63	0.2735836676772472
1106	64-65	0.29248488496466507
1106	66-67	0.6558913441130514
1106	68-69	0.7137749540720577
1106	70-71	0.5704797194737559
1106	72-73	0.6354747899591473
1106	74-75	0.4249350682113793
1106	76-77	0.39476315581897126
1106	78-79	0.3537578763843463
1106	80-81	0.21016530259529986
1106	82-83	0.36421608734900346
1106	84-85	0.42140854720152987
1106	86-87	0.5476122466060431
1106	88-89	0.3933364783989042
1106	90-91	0.4841461105179867
1106	92-93	0.3461515747098183
1106	94-95	0.21734697645809575
1106	96-97	-0.020855833260707612
1106	98-99	-0.1695055548443669
1106	100	-0.2950081572868406
1107	1	0.5143142538459315
1107	2	0.8727208718309818
1107	3	0.6855413686030616
1107	4	0.19356256113082182
1107	5	0.35582447003640993
1107	6	0.8057038878101679
1107	7	0.5549466304190069
1107	8	1.0642744188616824
1107	9	0.8597030447149692
1107	10-11	0.960271240829563
1107	12-13	1.0275832566987972
1107	14-15	0.7728074248574046
1107	16-17	0.9247598535396335
1107	18-19	0.8802987725451459
1107	20-21	0.17269550393137223
1107	22-23	0.21244935269908893
1107	24-25	0.8092052213198357
1107	26-27	0.5875305284489158
1107	28-29	0.8977751360748414
1107	30-31	0.6446515924826031
1107	32-33	0.7404564294177227
1107	34-35	0.7027454823697106
1107	36-37	0.9828501137586798
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2216	100	-0.24389898451049064
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6926.0
3	884.0
4	647.0
5	775.0
6	2050.0
7	4122.0
8	4125.0
9	2385.0
10	1398.0
11	1354.0
12	1248.0
13	760.0
14	612.0
15	530.0
16	540.0
17	572.0
18	489.0
19	392.0
20	347.0
21	349.0
22	424.0
23	748.0
24	1282.0
25	950.0
26	1034.0
27	849.0
28	898.0
29	1123.0
30	1137.0
31	1459.0
32	1835.0
33	2581.0
34	3712.0
35	6983.0
36	10468.0
37	19074.0
38	24789.0
39	8396.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.38192400094745	22.217710553920075	12.900551551449938	18.499813893682536
2	15.496519584210839	31.364339905102632	30.945675065337085	22.193465445349435
3	21.955387127062945	33.87499259835726	26.15952020436993	18.01010007020987
4	12.251165387771676	24.875424065786245	32.86012808690282	30.01328245953926
5	10.839693364696327	46.945492697950684	28.033777266342884	14.181036671010103
6	24.59218898064167	42.992757547042096	14.37660755381075	18.038445918505484
7	20.614788428507367	43.91938199632786	18.040901283559105	17.424928291605678
8	20.920353383204144	40.135565108485935	17.23166232271604	21.71241918559388
9	20.03587656431128	29.428090334481272	18.22766387720738	32.30836922400007
10-11	19.796320741944218	36.825582182507446	21.83861359328459	21.53948348226374
12-13	22.327673384418144	37.351854751014436	21.327415280592707	18.993056583974717
14-15	20.25939404197698	38.636171293161816	22.108158429248476	18.996276235612726
16-17	17.815700524986568	40.77043153811335	22.22711062791101	19.186757308989073
18-19	17.694318931817026	40.46132236719636	23.35846406390144	18.485894637085174
20-21	20.422201539893393	37.3309924697521	22.58989762247229	19.656908367882224
22-23	20.22212447767759	37.25152678858419	22.863341848386934	19.66300688535129
24-25	21.78363933123039	37.38022951956498	21.600970849154734	19.235160300049895
26-27	19.85986155507102	35.91396938969919	24.823980185863377	19.402188869366412
28-29	18.99184474561359	38.9992893761738	23.314383364634622	18.694482513577988
30-31	19.926897059383446	37.9526100034267	22.633567279665286	19.486925657524566
32-33	18.906948016786245	38.04360701231894	23.957205225395967	19.092239745498848
34-35	19.590043676246125	37.70955507731076	23.109934760457143	19.59046648598597
36-37	18.845660972649263	37.586435860890596	24.59790143245632	18.970001734003812
38-39	18.85971962537754	37.74228208360476	24.50909060144333	18.88890768957436
40-41	17.991226474557198	38.1174569466947	24.61832626177594	19.272990316972162
42-43	17.45869254393015	38.28416484065009	23.71889779101346	20.5382448244063
44-45	17.97147304659741	37.20982369462963	24.035142634767013	20.783560624005958
46-47	19.28485012563558	37.792197903535566	23.79884770598736	19.124104264841495
48-49	18.43104906937394	35.671319796954315	23.957698815566836	21.93993231810491
50-51	16.69959011365992	35.47906787870072	25.17311247128892	22.64822953635045
52-53	18.334369699200096	35.65775392021252	22.356315275186862	23.651561105400525
54-55	18.011741914744224	34.13064995051138	23.144599818964714	24.71300831577968
56-57	19.970982983169282	32.91908651385499	23.059645620164712	24.050284882811013
58-59	21.028009711612484	32.43492458273059	22.968640289656456	23.568425416000473
60-61	19.86566166704735	33.354059335583585	23.949530069622448	22.83074892774662
62-63	19.488188143730305	32.87777848275278	23.658397309815367	23.97563606370154
64-65	19.965399680221985	33.74715541380798	23.08281235459829	23.204632551371745
66-67	21.242819799165872	32.30688537903847	22.789259519317806	23.661035302477856
68-69	21.319803395737985	32.891030142038964	23.188136066392005	22.601030395831042
70-71	23.384483940006596	32.5615626030978	22.549127422533903	21.504826034361706
72-73	22.5428245146555	30.92120289303388	25.516643403967347	21.01932918834327
74-75	21.73778798529779	32.989041014774116	23.981186581904772	21.291984418023322
76-77	23.231391921097263	30.826034181599795	24.27012007122224	21.672453826080705
78-79	24.178793915018336	30.52767803900174	24.724784416221542	20.568743629758384
80-81	25.26622063960619	29.37731013541517	24.52190240973027	20.834566815248373
82-83	24.082015885502575	29.00584498523951	26.260562844164742	20.651576285093174
84-85	24.11744307994181	29.93250786562468	25.471937480970265	20.47811157346324
86-87	24.062248911066945	29.827462257368797	25.171903412695055	20.938385418869203
88-89	23.887476899525094	29.411714953883628	24.405519586242477	22.2952885603488
90-91	24.775774797975313	28.69025418532567	24.703041681995593	21.830929334703423
92-93	25.491605700511695	29.71497441535924	24.431006047278725	20.36241383685034
94-95	23.8527182922291	30.359196860516256	24.560624513684495	21.227460333570146
96-97	21.662677301215417	31.675702649897236	25.51995669494464	21.141663353942704
98-99	21.57199404560525	33.781463563163946	23.302912916976794	21.34362947425401
100	20.963949180355602	33.012468068549	23.7054017018829	22.318181049212498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1998.0
1	1299.5
2	582.5
3	504.5
4	380.5
5	269.5
6	230.5
7	249.0
8	276.5
9	292.5
10	224.5
11	157.5
12	136.0
13	117.5
14	114.0
15	108.5
16	110.5
17	121.0
18	136.0
19	158.0
20	195.0
21	243.5
22	329.5
23	432.5
24	577.5
25	740.5
26	903.0
27	1171.0
28	1435.0
29	1670.0
30	1863.5
31	2038.0
32	2438.0
33	3013.0
34	3320.5
35	3452.0
36	3614.5
37	3622.5
38	3427.0
39	3200.0
40	3025.5
41	2786.5
42	2602.5
43	2493.0
44	2616.5
45	2887.0
46	3528.0
47	5087.0
48	7492.5
49	7609.5
50	5525.5
51	4015.0
52	3024.5
53	2705.0
54	2673.0
55	2316.0
56	2125.5
57	1980.0
58	1451.0
59	1209.0
60	1089.0
61	901.5
62	741.5
63	606.0
64	513.5
65	472.5
66	444.0
67	398.5
68	306.5
69	285.0
70	343.5
71	252.5
72	118.5
73	95.0
74	81.5
75	81.0
76	74.5
77	51.5
78	33.0
79	22.5
80	17.0
81	9.0
82	7.5
83	6.0
84	5.5
85	4.5
86	1.0
87	1.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05073051948051947
2	0.032974837662337664
3	0.046502976190476185
4	0.06003111471861471
5	0.07271374458874459
6	0.06764069264069264
7	0.07186823593073592
8	0.0845508658008658
9	0.07525027056277056
10-11	0.08116883116883117
12-13	0.08666463744588745
14-15	0.0946969696969697
16-17	0.06637242965367965
18-19	0.07609577922077923
20-21	0.06933170995670997
22-23	0.0422754329004329
24-25	0.02113771645021645
26-27	0.14754126082251082
28-29	0.055803571428571425
30-31	0.06975446428571429
32-33	0.06764069264069264
34-35	0.0131053841991342
36-37	0.04100716991341991
38-39	0.06172213203463204
40-41	0.06383590367965368
42-43	0.06172213203463204
44-45	0.05749458874458875
46-47	0.06172213203463204
48-49	0.060876623376623376
50-51	0.05791734307359307
52-53	0.059608360389610385
54-55	0.05326704545454545
56-57	0.0553808170995671
58-59	0.05326704545454545
60-61	0.05326704545454545
62-63	0.05453530844155844
64-65	0.054958062770562775
66-67	0.054958062770562775
68-69	0.054958062770562775
70-71	0.049885010822510824
72-73	0.048193993506493504
74-75	0.04946225649350649
76-77	0.04269818722943723
78-79	0.0393161525974026
80-81	0.036356872294372296
82-83	0.04312094155844156
84-85	0.030438311688311688
86-87	0.031283820346320344
88-89	0.03255208333333333
90-91	0.026633522727272724
92-93	0.031283820346320344
94-95	0.030438311688311688
96-97	0.03466585497835498
98-99	0.03382034632034632
100	0.0422754329004329
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	118272.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.08661390692641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.86309848258401	39.365192099567096
2	7.481581769908392	7.19527867965368
3	2.996149315140752	4.32224025974026
4	1.7706117138185078	3.4057088744588744
5	1.1780634044274085	2.8324540043290045
6	0.9055263481792766	2.6126217532467533
7	0.5960649165684947	2.0063920454545454
8	0.46243384382747527	1.7789502164502164
9	0.31121973519947954	1.3468952922077921
>10	2.284036361718214	19.338474025974026
>50	0.0931900902009741	3.064123376623377
>100	0.04571589330613824	5.7240936147186146
>500	0.0070332143547904985	2.0655776515151514
>1k	0.005274910766092874	4.941998106060606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2919	2.468039772727273	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1750	1.4796401515151516	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1176	0.9943181818181818	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	855	0.7229099025974026	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	539	0.4557291666666667	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	534	0.4515016233766234	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	515	0.4354369588744589	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	481	0.4066896645021645	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	463	0.3914705086580087	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	458	0.3872429653679654	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	435	0.36779626623376627	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	380	0.32129329004329005	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	372	0.3145292207792208	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	366	0.30945616883116883	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	317	0.2680262445887446	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	306	0.25872564935064934	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	297	0.25111607142857145	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	294	0.24857954545454544	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.22236877705627706	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	238	0.2012310606060606	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	217	0.18347537878787878	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	214	0.18093885281385283	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	206	0.17417478354978355	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	201	0.16994724025974026	No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	196	0.16571969696969696	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	193	0.163183170995671	No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	181	0.1530370670995671	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	146	0.12344426406926408	No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.10399756493506493	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	4.22754329004329E-4	0.0
18-19	0.0	0.0	0.0	8.45508658008658E-4	0.0
20-21	0.0	0.0	0.0	0.001268262987012987	0.0
22-23	0.0	0.0	0.0	0.005495806277056277	0.0
24-25	0.0	0.0	0.0	0.02071496212121212	0.0
26-27	0.0	0.0	0.0	0.05453530844155845	0.0
28-29	0.0	0.0	0.0	0.10230654761904762	0.0
30-31	0.0	0.0	0.0	0.18262987012987014	0.0
32-33	0.0	0.0	0.0	0.3086106601731602	0.0
34-35	0.0	0.0	0.0	0.5584584686147186	0.0
36-37	0.0	0.0	0.0	0.8991984577922078	0.0
38-39	0.0	0.0	0.0	1.2991240530303032	0.0
40-41	0.0	0.0	0.0	1.7070819805194803	0.0
42-43	0.0	0.0	0.0	2.0423261634199132	0.0
44-45	0.0	0.0	0.0	2.3699607683982684	0.0
46-47	0.0	0.0	0.0	2.701822916666667	0.0
48-49	0.0	0.0	0.0	3.0383353625541125	0.0
50-51	0.0	0.0	0.0	3.372734036796537	0.0
52-53	0.0	0.0	0.0	3.6750033820346317	0.0
54-55	0.0	0.0	0.0	4.022507440476191	0.0
56-57	0.0	0.0	0.0	4.4439935064935066	0.0
58-59	0.0	0.0	0.0	4.792343073593074	0.0
60-61	0.0	0.0	0.0	5.158448322510823	0.0
62-63	0.0	0.0	0.0	5.541463744588745	0.0
64-65	0.0	0.0	0.0	5.935470779220779	0.0
66-67	0.0	0.0	0.0	6.409378382034632	0.0
68-69	0.0	0.0	0.0	6.951349431818182	0.0
70-71	0.0	0.0	0.0	7.473873782467532	0.0
72-73	0.0	0.0	0.0	7.971878382034632	0.0
74-75	0.0	0.0	0.0	8.538791937229437	0.0
76-77	0.0	0.0	0.0	9.10655100108225	0.0
78-79	0.0	0.0	0.0	9.779575892857142	0.0
80-81	0.0	0.0	0.0	10.49910376082251	0.0
82-83	0.0	0.0	0.0	11.1425358495671	0.0
84-85	0.0	0.0	0.0	11.829511634199134	0.0
86-87	0.0	0.0	0.0	12.532552083333332	0.0
88	0.0	0.0	0.0	13.089319534632034	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAAAT	25	0.0052002748	56.4661	3
TGCTATA	25	0.0052002748	56.4661	2
CGCAGAA	145	0.0	55.16803	2
TACATGA	70	8.442839E-8	47.055084	4
GAGAACA	250	0.0	43.30903	1
GGAAACA	220	0.0	42.795483	1
GTAACAA	45	0.0010023566	41.84447	1
TAACAAC	160	0.0	41.19065	1
TGGACAG	60	8.71567E-5	39.21257	5
CCGTATC	50	0.001703751	37.56448	94
GCAGAAA	180	0.0	36.598396	3
TGTGTAC	55	0.0026916824	34.22188	8
AGAACAA	240	0.0	33.330685	2
ACAACAC	455	0.0	33.093685	5
CACAAAG	115	1.102926E-7	32.761734	9
ACATGGA	130	8.938514E-9	32.576595	3
GGGTATC	30	0.0041305986	31.370056	22-23
ATCAACC	60	0.0041223057	31.370056	8
ACAGCAG	60	0.0041223057	31.370056	8
CGCCGTA	90	2.5911311E-5	31.303732	94
>>END_MODULE