FastQCFastQC Report
Wed 25 May 2016
SRR1033098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4971288
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59400.034.9003831
TCAACGC56200.034.7936554
GGTATCA36600.034.6785351
CAACGCA58000.033.794565
ATCAACG59350.033.1050533
AACGCAG61650.031.7136696
TATCAAC68750.029.1940172
ACGCAGA75150.025.8297637
GTACATG126850.025.607411
CGCAGAG77050.024.7837878
TACATGG127350.024.397272
ACATGGG130400.022.8533763
TACGTCG1656.859723E-519.9412695
GCAGAGT98800.018.3762869
CATGGGG97050.018.1626324
GAGTACA96000.017.6772211
GTATAGA23350.017.3136881
AGAGTAC83900.016.46021310-11
GTACTAG9500.016.329331
AGTACAT94500.016.0660612
Produced by FastQC (version 0.11.5)