FastQCFastQC Report
Wed 25 May 2016
SRR1033098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4971288
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.11902348043404445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50150.10087928923047709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.049.765091
GTATCAA105350.042.7300031
TCAACGC123700.035.4485634
ATCAACG126050.034.863653
CAACGCA126750.034.6315965
TATCAAC133050.033.360252
AACGCAG132400.033.2243966
ACGCAGA153800.028.2653627
CGCAGAG156050.027.7975888
GTACATG121350.025.5982151
TACATGG121450.024.4419732
GCAGAGT179900.023.511519
ACATGGG125250.022.9280763
AGAGTAC165450.020.70760710-11
GAGTACT118400.019.78712312-13
CAGAGTA177650.019.1135710-11
CATGGGG94200.018.9092794
AGTACTT128300.018.86469512-13
GTGGTAT30550.018.5154421
GTACTTT131250.017.63502714-15
Produced by FastQC (version 0.11.5)