##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4971288 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.700988757843035 34.0 31.0 34.0 31.0 34.0 2 32.705911828081575 34.0 31.0 34.0 31.0 34.0 3 32.80877752405413 34.0 31.0 34.0 31.0 34.0 4 36.29118308977472 37.0 37.0 37.0 35.0 37.0 5 36.273696675791065 37.0 37.0 37.0 35.0 37.0 6 36.336258329833235 37.0 37.0 37.0 35.0 37.0 7 36.285473100733654 37.0 37.0 37.0 35.0 37.0 8 36.294834256232996 37.0 37.0 37.0 35.0 37.0 9 38.101613505393374 39.0 39.0 39.0 37.0 39.0 10-11 38.059366506225345 39.0 39.0 39.0 35.0 39.0 12-13 38.03281201974217 39.0 39.0 39.0 35.0 39.0 14-15 39.52455892718346 41.0 39.0 41.0 37.0 41.0 16-17 39.52540267230545 41.0 39.0 41.0 37.0 41.0 18-19 39.538728393929304 41.0 39.5 41.0 37.0 41.0 20-21 39.52827848638019 41.0 39.5 41.0 37.0 41.0 22-23 39.45662472180248 41.0 39.0 41.0 36.0 41.0 24-25 39.40360908480861 41.0 39.0 41.0 36.0 41.0 26-27 39.295342776358964 41.0 39.0 41.0 36.0 41.0 28-29 39.24603090788544 41.0 39.0 41.0 36.0 41.0 30-31 39.251583995938276 41.0 39.0 41.0 36.0 41.0 32-33 39.22308464124387 41.0 39.0 41.0 35.5 41.0 34-35 39.149446883785444 41.0 39.0 41.0 35.0 41.0 36-37 39.06581030911909 41.0 39.0 41.0 35.0 41.0 38-39 38.97832734695717 41.0 39.0 41.0 35.0 41.0 40-41 38.88344630204486 41.0 39.0 41.0 35.0 41.0 42-43 38.776629819073044 41.0 39.0 41.0 35.0 41.0 44-45 38.62324964878317 41.0 38.5 41.0 35.0 41.0 46-47 38.44705486787328 41.0 38.0 41.0 34.5 41.0 48-49 38.26411666352865 40.0 38.0 41.0 34.0 41.0 50-51 38.13423060583092 40.0 38.0 41.0 34.0 41.0 52-53 37.99881147501412 40.0 38.0 41.0 34.0 41.0 54-55 37.85292584135138 40.0 37.5 41.0 33.5 41.0 56-57 37.7023463537015 40.0 37.0 41.0 33.0 41.0 58-59 37.525329250689154 40.0 37.0 41.0 33.0 41.0 60-61 37.33224920785116 40.0 36.0 41.0 33.0 41.0 62-63 37.10041924748677 39.0 36.0 41.0 33.0 41.0 64-65 36.82962342958203 39.0 35.0 41.0 32.0 41.0 66-67 36.54738349498158 39.0 35.0 41.0 32.0 41.0 68-69 36.23943291959749 38.0 35.0 40.5 31.5 41.0 70-71 35.91152836045708 37.0 35.0 40.0 31.0 41.0 72-73 35.55860060813214 37.0 35.0 39.0 31.0 41.0 74-75 35.20743195727144 36.0 35.0 39.0 31.0 41.0 76-77 34.850841069758985 36.0 35.0 39.0 31.0 40.5 78-79 34.48472116280529 35.0 34.5 37.0 30.5 39.5 80-81 34.15054046355793 35.0 34.0 37.0 30.0 39.0 82-83 33.8497106786008 35.0 34.0 36.5 30.0 39.0 84-85 33.62572627053592 35.0 34.0 36.0 30.0 37.0 86-87 33.415432278314995 35.0 34.0 36.0 30.0 37.0 88-89 33.20867419067252 35.0 34.0 35.5 30.0 37.0 90-91 33.02869356995612 35.0 34.0 35.0 30.0 36.0 92-93 32.85606878941634 35.0 34.0 35.0 29.5 36.0 94-95 32.70243023538367 35.0 34.0 35.0 29.0 36.0 96-97 32.5611401914353 35.0 34.0 35.0 29.0 36.0 98-99 32.438563406505516 35.0 34.0 35.0 29.0 35.5 100 32.33632109022853 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1000948404541333 1101 2 0.0749634379858719 1101 3 0.14173033593417728 1101 4 0.11076814110290911 1101 5 0.08634490128387995 1101 6 0.12821948918126935 1101 7 0.16141141930974356 1101 8 0.15557471329213257 1101 9 0.1877416391838409 1101 10-11 0.21493617492861716 1101 12-13 0.2145910014976451 1101 14-15 0.3078759299531626 1101 16-17 0.2937982709061373 1101 18-19 0.2845181623961537 1101 20-21 0.2851326292366707 1101 22-23 0.2877756341072626 1101 24-25 0.29556417640201005 1101 26-27 0.3193764336292517 1101 28-29 0.33576812313800986 1101 30-31 0.30605228731575096 1101 32-33 0.3384612656705457 1101 34-35 0.34094894964066214 1101 36-37 0.3014394073843434 1101 38-39 0.29367989171647935 1101 40-41 0.32892988012613245 1101 42-43 0.33605039794208125 1101 44-45 0.3456535461096948 1101 46-47 0.32614036424877213 1101 48-49 0.25091726225727484 1101 50-51 0.25873992446918237 1101 52-53 0.24864751309022637 1101 54-55 0.22516780635855582 1101 56-57 0.19799528951001832 1101 58-59 0.18670277382039302 1101 60-61 0.17651437677954362 1101 62-63 0.19681951009993526 1101 64-65 0.19680248486000096 1101 66-67 0.2509371448047517 1101 68-69 0.20735211422755384 1101 70-71 0.22364595739621507 1101 72-73 0.1960021807442729 1101 74-75 0.1634765058136054 1101 76-77 0.19684869936518368 1101 78-79 0.20663484220492734 1101 80-81 0.1886273511248504 1101 82-83 0.20175583812790876 1101 84-85 0.16569176931660223 1101 86-87 0.22610854083914944 1101 88-89 0.2645676092138629 1101 90-91 0.25622985099859363 1101 92-93 0.24579615549301792 1101 94-95 0.2807392960750619 1101 96-97 0.31557341157454033 1101 98-99 0.3055311925651125 1101 100 0.28912885142117517 1102 1 0.06255887508127955 1102 2 -7.438438149378612E-4 1102 3 0.08286568252546544 1102 4 0.06096362012998213 1102 5 0.12265620651240994 1102 6 0.08789495283930648 1102 7 0.13581166515137966 1102 8 0.12468605674672517 1102 9 0.17076838075067968 1102 10-11 0.1557671532434668 1102 12-13 0.20916287831247615 1102 14-15 0.2897678212835615 1102 16-17 0.25159067430742255 1102 18-19 0.24563809447414542 1102 20-21 0.22769279218928773 1102 22-23 0.22967915159554764 1102 24-25 0.2981803005567585 1102 26-27 0.23126022438109572 1102 28-29 0.24046971813839235 1102 30-31 0.2490961435796919 1102 32-33 0.247431161468036 1102 34-35 0.2479931402141915 1102 36-37 0.29043951850270133 1102 38-39 0.23714034354875224 1102 40-41 0.29846605897648715 1102 42-43 0.2677583572335678 1102 44-45 0.3535800214697531 1102 46-47 0.32503617122378614 1102 48-49 0.3687240402972165 1102 50-51 0.38129906158435745 1102 52-53 0.3451697360030792 1102 54-55 0.31823727486975173 1102 56-57 0.3274175431306219 1102 58-59 0.2960311063224452 1102 60-61 0.28275973643720675 1102 62-63 0.2599255269482583 1102 64-65 0.284815240056723 1102 66-67 0.2888665022795678 1102 68-69 0.28838020493629557 1102 70-71 0.24926686559196298 1102 72-73 0.3197525975784785 1102 74-75 0.3180949866432172 1102 76-77 0.23738261435453012 1102 78-79 0.2915192283018442 1102 80-81 0.29860437000750295 1102 82-83 0.21980033027631407 1102 84-85 0.27153558126796895 1102 86-87 0.31092695216784705 1102 88-89 0.2899153959063767 1102 90-91 0.23265936841309554 1102 92-93 0.2892740330864072 1102 94-95 0.35529851864860973 1102 96-97 0.30915168030566065 1102 98-99 0.3035161968091131 1102 100 0.3480666947110578 1103 1 0.11591225679392636 1103 2 0.05957511872264121 1103 3 0.07188596612218845 1103 4 0.05285909043819714 1103 5 0.09259464339343992 1103 6 0.06805448496450595 1103 7 0.08531263206214135 1103 8 0.07038369441952597 1103 9 0.10242232066588741 1103 10-11 0.12566885614820222 1103 12-13 0.1240779393705509 1103 14-15 0.187916332381306 1103 16-17 0.23220783588389793 1103 18-19 0.19972687031054193 1103 20-21 0.22716096453952872 1103 22-23 0.219849833271482 1103 24-25 0.1463203342821231 1103 26-27 0.15942121564190614 1103 28-29 0.15573100702916776 1103 30-31 0.07296726363006201 1103 32-33 0.08486000063604138 1103 34-35 0.16027602996096846 1103 36-37 0.14030221532319587 1103 38-39 0.17229672531493634 1103 40-41 0.16501252716962256 1103 42-43 0.1878275981056774 1103 44-45 0.17433493454453242 1103 46-47 0.16917471827284203 1103 48-49 0.15401222753455812 1103 50-51 0.1895637185517387 1103 52-53 0.18285538700255444 1103 54-55 0.19804481282773878 1103 56-57 0.18203043730659374 1103 58-59 0.16981296815684743 1103 60-61 0.1844737252543709 1103 62-63 0.16735242496621794 1103 64-65 0.17715755304121217 1103 66-67 0.18711023222535772 1103 68-69 0.2092874681679504 1103 70-71 0.21161013232973858 1103 72-73 0.20604453648015664 1103 74-75 0.23488250248466613 1103 76-77 0.2334493535085329 1103 78-79 0.2508402811421959 1103 80-81 0.1898874874084271 1103 82-83 0.1779448917869999 1103 84-85 0.20808649796258294 1103 86-87 0.1942412873719448 1103 88-89 0.19516204049823216 1103 90-91 0.18705467800201347 1103 92-93 0.24386617002295452 1103 94-95 0.2318940309381361 1103 96-97 0.2677289516266228 1103 98-99 0.24687307040112216 1103 100 0.2579106935855364 1104 1 0.10547310723934089 1104 2 0.09975739665658523 1104 3 0.11991570528398654 1104 4 0.0443466301314146 1104 5 0.04673849510255934 1104 6 0.059694263624237465 1104 7 0.11115514795707071 1104 8 0.0916288417416311 1104 9 0.11283234137729892 1104 10-11 0.1423880942525173 1104 12-13 0.15457630053852256 1104 14-15 0.1711286402787806 1104 16-17 0.18949679960523014 1104 18-19 0.15330406016319387 1104 20-21 0.18325592554167258 1104 22-23 0.17781159539858038 1104 24-25 0.171472311648742 1104 26-27 0.1455854774958496 1104 28-29 0.17001226803309066 1104 30-31 0.1869367705738938 1104 32-33 0.19496649247449227 1104 34-35 0.16055466425868303 1104 36-37 0.20130036124921702 1104 38-39 0.2324025114420678 1104 40-41 0.23075956275621223 1104 42-43 0.20001879769080944 1104 44-45 0.21653535217963338 1104 46-47 0.2264795970012159 1104 48-49 0.14527090991311553 1104 50-51 0.2416543031702929 1104 52-53 0.22286068957141225 1104 54-55 0.21371300342527633 1104 56-57 0.2686928096793082 1104 58-59 0.20699316229559628 1104 60-61 0.23402653545769425 1104 62-63 0.18366340907092393 1104 64-65 0.14932247339682192 1104 66-67 0.21578846655510375 1104 68-69 0.22103275553725155 1104 70-71 0.24603251613011423 1104 72-73 0.25093687778453955 1104 74-75 0.21724908836765877 1104 76-77 0.19221367975254822 1104 78-79 0.1611962784351917 1104 80-81 0.14259412150854445 1104 82-83 0.14541849879194046 1104 84-85 0.17169516740715807 1104 86-87 0.17208861962497224 1104 88-89 0.17038085359399702 1104 90-91 0.22781498217819518 1104 92-93 0.1965416554854258 1104 94-95 0.24733037103162303 1104 96-97 0.2937793968452951 1104 98-99 0.2590005612883175 1104 100 0.32213507182344614 1105 1 0.03732712304031338 1105 2 0.07108152611014873 1105 3 0.10366484860335845 1105 4 0.04760615724818251 1105 5 0.06952855004202974 1105 6 0.09421387114699797 1105 7 0.07055003038982477 1105 8 0.0654527552064792 1105 9 0.05821633797877723 1105 10-11 0.12351596270576692 1105 12-13 0.12316813916428515 1105 14-15 0.16144889894597014 1105 16-17 0.1909598825453429 1105 18-19 0.2022304059967155 1105 20-21 0.1623970111613957 1105 22-23 0.20304507584297937 1105 24-25 0.1832161664899985 1105 26-27 0.14890339990375168 1105 28-29 0.19705056361577533 1105 30-31 0.15464247352650773 1105 32-33 0.15979766539841478 1105 34-35 0.1758915417467506 1105 36-37 0.154346473609003 1105 38-39 0.15942724338104597 1105 40-41 0.11232057251800853 1105 42-43 0.12577852960517788 1105 44-45 0.09529515616483764 1105 46-47 0.10157244976674207 1105 48-49 0.09616163394757393 1105 50-51 0.14640554012535745 1105 52-53 0.19486412032274814 1105 54-55 0.15519393844826368 1105 56-57 0.1643995135661669 1105 58-59 0.14036151638995875 1105 60-61 0.1480755158790501 1105 62-63 0.17711798854649174 1105 64-65 0.16324378546224239 1105 66-67 0.10581636826837126 1105 68-69 0.14506851700155465 1105 70-71 0.07595212356334713 1105 72-73 0.1211896360611604 1105 74-75 0.0967580486539461 1105 76-77 0.14644629758502248 1105 78-79 0.1466204646053768 1105 80-81 0.15190614485054255 1105 82-83 0.15688136686245002 1105 84-85 0.1444729344911977 1105 86-87 0.11818699051049464 1105 88-89 0.14246116737523806 1105 90-91 0.1404496729651683 1105 92-93 0.18219787844486746 1105 94-95 0.14215561713565705 1105 96-97 0.15706292668731692 1105 98-99 0.10791882712946688 1105 100 0.1648860204951248 1106 1 0.0522457592289598 1106 2 0.016177594060280853 1106 3 0.044122560014855594 1106 4 0.036741460063502984 1106 5 0.032554574431607364 1106 6 0.06054075626483524 1106 7 0.08600054569244975 1106 8 0.08595891448824489 1106 9 0.06796693106308282 1106 10-11 0.08310365772776862 1106 12-13 0.04459335262541231 1106 14-15 0.10634811329387617 1106 16-17 0.08089873775218592 1106 18-19 0.09084541710879535 1106 20-21 0.07828405454977627 1106 22-23 0.08432266213361572 1106 24-25 0.08163091686258639 1106 26-27 0.07857437370047649 1106 28-29 0.06752521639266007 1106 30-31 0.08539722806742134 1106 32-33 0.07258599687167333 1106 34-35 0.03705295276490972 1106 36-37 -7.663048684136697E-4 1106 38-39 -0.013799541483550115 1106 40-41 0.014298317243003567 1106 42-43 -0.013247977310491876 1106 44-45 0.023651759330064692 1106 46-47 0.03526894217766596 1106 48-49 0.10493190974450783 1106 50-51 0.04405269176108817 1106 52-53 0.08746685961808254 1106 54-55 0.11694967070022955 1106 56-57 0.0974683580096638 1106 58-59 0.13697973822007725 1106 60-61 0.12601688763486862 1106 62-63 0.11025114084427656 1106 64-65 0.1277446915638336 1106 66-67 0.11703187317542074 1106 68-69 0.10962322327331009 1106 70-71 0.09539336628976969 1106 72-73 0.10168857399904851 1106 74-75 0.1841008977570553 1106 76-77 0.15711817462889854 1106 78-79 0.06632971946743993 1106 80-81 0.14833218775680734 1106 82-83 0.12267955826661847 1106 84-85 0.1303521790267652 1106 86-87 0.034130171408222054 1106 88-89 0.07303758086766265 1106 90-91 0.05965513815234402 1106 92-93 0.02190112986156123 1106 94-95 0.042357914876589575 1106 96-97 0.03230972281582467 1106 98-99 0.025629553563732088 1106 100 0.023329064668850208 1107 1 -0.011951087941980632 1107 2 -0.009442013888730116 1107 3 0.023087184114636727 1107 4 0.02828583142115093 1107 5 0.04770927055523089 1107 6 0.053836003118242104 1107 7 0.024464125757098998 1107 8 0.009567282147656897 1107 9 0.04544407681576956 1107 10-11 0.015077747029216937 1107 12-13 0.035147838046782454 1107 14-15 0.028466585231484487 1107 16-17 0.03616579886735849 1107 18-19 0.06161330372843565 1107 20-21 0.061663945875437776 1107 22-23 0.09871886342844505 1107 24-25 0.06504652731700133 1107 26-27 0.05322214673367398 1107 28-29 0.04242594351577367 1107 30-31 0.008404133559366755 1107 32-33 -0.041197142724875846 1107 34-35 -0.02573576396650168 1107 36-37 -0.015311513140524369 1107 38-39 -0.05296596260940589 1107 40-41 -0.04994420864050397 1107 42-43 -0.04479645781837149 1107 44-45 -0.05036363234613361 1107 46-47 -0.07692594355476956 1107 48-49 -0.13908872357363578 1107 50-51 -0.09374424211044641 1107 52-53 -0.10463324491306736 1107 54-55 -0.08294281220816657 1107 56-57 -0.07482917370764142 1107 58-59 -0.10299776820491502 1107 60-61 -0.0682613682072244 1107 62-63 -0.08836061694142217 1107 64-65 -0.10329458153120186 1107 66-67 -0.0979068334184845 1107 68-69 -0.13458882744406964 1107 70-71 -0.10991968296193022 1107 72-73 -0.074142192598039 1107 74-75 -0.07043589573036968 1107 76-77 -0.11549468331370605 1107 78-79 -0.09589260056714011 1107 80-81 -0.07525310074274216 1107 82-83 -0.07916997319200902 1107 84-85 -0.050290207554077426 1107 86-87 -0.03956740317977392 1107 88-89 -0.030670153656167543 1107 90-91 -0.13852342608488044 1107 92-93 -0.06514029512242558 1107 94-95 -0.08402297658467717 1107 96-97 -0.06825656558262949 1107 98-99 -0.032142530469315034 1107 100 -0.03329149808514842 1108 1 -0.05853947099900836 1108 2 -0.014551780481554033 1108 3 0.004660298234014704 1108 4 0.008729923774446036 1108 5 0.02782105195537099 1108 6 0.030211881192066414 1108 7 0.023724493550545844 1108 8 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58-59 -0.23630246990151704 2214 60-61 -0.26872406871925136 2214 62-63 -0.28990729967570417 2214 64-65 -0.27341998743794704 2214 66-67 -0.27507403566100663 2214 68-69 -0.2904062769149718 2214 70-71 -0.21850464827360838 2214 72-73 -0.2108596519063184 2214 74-75 -0.21784252181209496 2214 76-77 -0.23968303176003758 2214 78-79 -0.2101003278011433 2214 80-81 -0.16301375043191513 2214 82-83 -0.16171622560964494 2214 84-85 -0.13790783820881103 2214 86-87 -0.18282097846850576 2214 88-89 -0.1721944444937904 2214 90-91 -0.14565469298666045 2214 92-93 -0.21022861079767807 2214 94-95 -0.20178938820421166 2214 96-97 -0.13042958344792055 2214 98-99 -0.15147572421196287 2214 100 -0.1705237568225968 2215 1 -0.17624063486071861 2215 2 -0.1848961174878525 2215 3 -0.20868889638939692 2215 4 -0.10051623076480354 2215 5 -0.1453463773268595 2215 6 -0.1426296038523347 2215 7 -0.16937963297872471 2215 8 -0.1340570840851143 2215 9 -0.214059629815182 2215 10-11 -0.1848756244943175 2215 12-13 -0.2029986316511767 2215 14-15 -0.2900113343750803 2215 16-17 -0.2714054359468747 2215 18-19 -0.2752123336091614 2215 20-21 -0.2873086602988124 2215 22-23 -0.3015353805520604 2215 24-25 -0.34547803797390486 2215 26-27 -0.3656601009775571 2215 28-29 -0.3444296761212442 2215 30-31 -0.3596162888200425 2215 32-33 -0.31855870366333505 2215 34-35 -0.30751300079757016 2215 36-37 -0.29490203143424054 2215 38-39 -0.2737526343566117 2215 40-41 -0.2329352627794563 2215 42-43 -0.2418037848215775 2215 44-45 -0.2189904605690458 2215 46-47 -0.2509556550215848 2215 48-49 -0.22469918998828575 2215 50-51 -0.21120433910052583 2215 52-53 -0.20805035295231988 2215 54-55 -0.24585184987937936 2215 56-57 -0.22976644625441622 2215 58-59 -0.22239824771345695 2215 60-61 -0.19190806141607197 2215 62-63 -0.1883227595617072 2215 64-65 -0.20351166412029897 2215 66-67 -0.15818829876213414 2215 68-69 -0.196635801393775 2215 70-71 -0.2235925212181371 2215 72-73 -0.19655810994926526 2215 74-75 -0.19793698040348318 2215 76-77 -0.20377667618360107 2215 78-79 -0.1593211250067199 2215 80-81 -0.14748665819709572 2215 82-83 -0.15409251161152326 2215 84-85 -0.15070093381897465 2215 86-87 -0.14027217080380439 2215 88-89 -0.15421348986131989 2215 90-91 -0.16099684741488574 2215 92-93 -0.19807170525556472 2215 94-95 -0.1640674409211158 2215 96-97 -0.1804105874661417 2215 98-99 -0.17897182695173086 2215 100 -0.16929544998347978 2216 1 -0.16961517581112417 2216 2 -0.12475569058437941 2216 3 -0.11652217172214563 2216 4 -0.1321350825828418 2216 5 -0.15249990490851673 2216 6 -0.1368111412602957 2216 7 -0.1504539000746803 2216 8 -0.14552029083412066 2216 9 -0.17938244955343663 2216 10-11 -0.22206513938828465 2216 12-13 -0.2197792620437724 2216 14-15 -0.27830691078632697 2216 16-17 -0.27298890488773964 2216 18-19 -0.2531334042783584 2216 20-21 -0.29323106684520894 2216 22-23 -0.3066368980771301 2216 24-25 -0.2718682180034051 2216 26-27 -0.3302129647987684 2216 28-29 -0.34183797160057594 2216 30-31 -0.289270428014504 2216 32-33 -0.29523100190251483 2216 34-35 -0.29828797957055997 2216 36-37 -0.22727993478174113 2216 38-39 -0.25913622173654716 2216 40-41 -0.29392173831669055 2216 42-43 -0.25811452356370523 2216 44-45 -0.32076377502990283 2216 46-47 -0.26115085130358295 2216 48-49 -0.2241594105255018 2216 50-51 -0.2217966870423993 2216 52-53 -0.23592842183948193 2216 54-55 -0.25855825692201506 2216 56-57 -0.30423936181830413 2216 58-59 -0.27893994338671746 2216 60-61 -0.2432779550914006 2216 62-63 -0.26536263558299567 2216 64-65 -0.37552574322786114 2216 66-67 -0.3099876020448633 2216 68-69 -0.2840990574042408 2216 70-71 -0.3023467830524851 2216 72-73 -0.29415481541512634 2216 74-75 -0.34311913561970897 2216 76-77 -0.3195594276146423 2216 78-79 -0.35767142508608885 2216 80-81 -0.33465726938226226 2216 82-83 -0.34292708549759965 2216 84-85 -0.44044747376782567 2216 86-87 -0.4309812359254863 2216 88-89 -0.5544096328398425 2216 90-91 -0.5194625243391684 2216 92-93 -0.5148988469316862 2216 94-95 -0.40373144903021796 2216 96-97 -0.4422250276934747 2216 98-99 -0.5261749979242154 2216 100 -0.5233150909520354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 8.0 4 20.0 5 38.0 6 101.0 7 248.0 8 620.0 9 1206.0 10 2081.0 11 3162.0 12 4890.0 13 7397.0 14 8867.0 15 11595.0 16 13691.0 17 19299.0 18 22576.0 19 12366.0 20 8813.0 21 8632.0 22 9094.0 23 10040.0 24 11795.0 25 14383.0 26 18589.0 27 23995.0 28 31497.0 29 40635.0 30 52323.0 31 66725.0 32 86321.0 33 111773.0 34 153864.0 35 234674.0 36 423175.0 37 957865.0 38 1763176.0 39 833185.0 40 2546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.435111423709955 19.81952448081592 13.746547409538357 23.99881668593577 2 15.540141792550921 20.368500141437696 40.02592049931237 24.065437566699014 3 17.979217170848518 25.492388741940204 30.379574192793058 26.148819894418224 4 12.259819929569844 17.016611394590324 39.087409271788545 31.636159404051284 5 12.574088076730355 37.81942232572342 36.06651326549969 13.53997633204654 6 30.712679775164496 38.47090457003117 17.657025485795508 13.15939016900882 7 26.825663691180235 32.58978357319069 22.897124447426904 17.687428288202174 8 25.054221557066906 36.05650065275071 20.53923846421538 18.350039325967007 9 25.113451483800574 16.220182777581986 21.321214944698436 37.345150793919004 10-11 23.836160773599968 26.553544917210488 29.216174237010538 20.39412007217901 12-13 24.805513178878392 23.883367851550748 29.232504735191362 22.0786142343795 14-15 21.898094553208182 25.69172898300738 26.64775133139889 25.76242513238555 16-17 21.20563882773486 29.16699647766879 27.10774877664844 22.51961591794792 18-19 21.079085178796994 27.880812666148557 29.62895873717492 21.411143417879526 20-21 22.29695805191733 27.17697103849143 29.18843164990642 21.337639259684813 22-23 21.800927990096717 26.989700744164256 29.295152567803694 21.914218697935333 24-25 21.702889276340702 27.135642052189326 29.26448246434105 21.896986207128926 26-27 21.46474922660779 27.09883026125459 29.35649785236441 22.079922659773203 28-29 21.50021951120161 27.150261829340476 29.42979890963531 21.919719749822605 30-31 21.838149596019697 27.5828951163905 28.911365317839543 21.66758996975026 32-33 21.444100603304413 27.244810600391688 29.42499006293741 21.88609873336648 34-35 21.64813223454364 27.01761595787651 29.419880723064125 21.914371084515725 36-37 21.94590214849753 27.44673010294314 28.76798729021533 21.839380458343992 38-39 21.847778684316822 27.551491685856867 28.900689318341644 21.70004031148467 40-41 21.708842566395838 27.305536619017666 29.03822815961192 21.947392654974582 42-43 21.665170072625042 27.235517234165474 28.895791191337132 22.203521501872352 44-45 21.845646440117733 27.370351506490877 28.77412252116554 22.009879532225852 46-47 21.919963196660504 27.598521751304695 28.501356187772664 21.98015886426214 48-49 21.944403542904777 27.55213538221885 28.30922288147458 22.19423819340179 50-51 21.990769796479302 27.506986117078714 28.184225094180825 22.31801899226116 52-53 22.02851655345657 27.491446884590072 28.167961703284945 22.312074858668417 54-55 22.088923433926983 27.85737820862521 27.994857670688162 22.05884068675965 56-57 21.867964599918572 27.576957923178057 28.12532687705882 22.429750599844546 58-59 21.729449189023047 27.225831615468664 28.509583431899337 22.53513576360895 60-61 21.96517280833458 27.66323335119591 28.09873417110415 22.272859669365364 62-63 21.84762781798198 27.61612282370283 28.122400070162907 22.413849288152285 64-65 21.87915888196379 27.33680889137785 28.252165233637637 22.53186699302072 66-67 21.731279700552452 27.48833903809234 28.09371535103177 22.68666591032344 68-69 22.178140068133448 27.56434543382093 28.0257664359042 22.23174806214142 70-71 22.125783952080006 27.3378960695482 28.06865265848716 22.467667319884633 72-73 21.98134701810071 27.513284081661638 28.08983701245728 22.415531887780375 74-75 22.039444404157443 27.565641855897947 27.942486792373504 22.4524269475711 76-77 22.026424540280104 27.53772261836369 27.852047598127488 22.583805243228717 78-79 22.118312195954047 27.636167930725396 27.929995204462106 22.31552466885845 80-81 22.072730447320694 27.379504064137905 28.026801102651866 22.520964385889535 82-83 21.862419926298653 27.45590948969979 28.154723547529365 22.5269470364722 84-85 21.914652701674093 27.407736184264518 27.95850894174709 22.719102172314297 86-87 21.997880415995265 27.518373868508345 27.851207093736928 22.63253862175946 88-89 21.76494585470074 27.385373356445303 27.95882088711269 22.890859901741266 90-91 21.70954546154034 27.302843690415735 28.003906932451528 22.983703915592397 92-93 21.811706034380098 27.46700627287152 27.88934488884884 22.831942803899544 94-95 21.59851417771049 27.242472422451158 28.0426327039415 23.11638069589685 96-97 21.579346292055636 27.250802209173024 28.060926861374714 23.108924637396626 98-99 21.583936766360683 27.3161649781002 27.91522622552993 23.184672030009185 100 21.690093191140807 27.1925505020027 27.83469796962075 23.28265833723574 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23.0 1 18.0 2 75.5 3 134.5 4 92.0 5 77.5 6 202.5 7 482.0 8 838.0 9 999.0 10 897.0 11 850.5 12 956.0 13 1215.0 14 1536.0 15 1794.5 16 2096.0 17 2544.0 18 2977.0 19 3481.5 20 4261.0 21 5496.0 22 7301.0 23 10185.5 24 14073.0 25 18806.0 26 25116.0 27 33362.0 28 44530.5 29 57511.0 30 70525.5 31 84586.5 32 99815.5 33 116296.0 34 132723.0 35 145115.5 36 155421.5 37 165906.0 38 174572.5 39 179641.5 40 183828.0 41 187751.5 42 188276.0 43 186359.0 44 183588.0 45 181592.0 46 177584.5 47 170483.5 48 165315.0 49 160761.0 50 155593.0 51 149901.5 52 140000.5 53 132156.0 54 127395.5 55 121154.0 56 112675.5 57 103538.0 58 94471.5 59 84137.5 60 73550.0 61 62930.0 62 53311.0 63 44472.5 64 36390.0 65 29813.5 66 23946.0 67 18211.0 68 14271.0 69 11532.0 70 8739.5 71 6317.0 72 4546.5 73 3348.0 74 2438.5 75 1608.0 76 1009.0 77 668.0 78 450.5 79 271.5 80 167.5 81 95.5 82 49.0 83 22.5 84 13.0 85 8.0 86 7.5 87 6.0 88 4.5 89 2.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04411331630756456 2 0.23146918866901295 3 0.003319059366506225 4 0.0024540923800833907 5 1.8103960180943047E-4 6 1.8103960180943047E-4 7 0.0 8 3.6207920361886094E-4 9 0.0 10-11 7.342161628938013E-4 12-13 0.0 14-15 2.6150164705806623E-4 16-17 1.3075082352903311E-4 18-19 4.3248349321141724E-4 20-21 0.0 22-23 1.3075082352903311E-4 24-25 0.001216988434385616 26-27 1.408085791851126E-4 28-29 3.117904253384636E-4 30-31 1.2069306787295365E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.0057755656079471E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.9167491402630464E-4 70-71 4.023102262431789E-5 72-73 3.721369592749404E-4 74-75 2.5144389140198674E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.70981846153351E-4 84-85 0.0 86-87 5.934075837086888E-4 88-89 0.0025546699366441857 90-91 0.002504381158363788 92-93 8.046204524863577E-4 94-95 6.83927384613404E-4 96-97 3.218481809945431E-4 98-99 2.0115511312158941E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4971288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.66126522799992 #Duplication Level Percentage of deduplicated Percentage of total 1 68.42598869733841 36.71825127976001 2 16.796628614034425 18.026566859878283 3 6.302607164565212 10.146176240568792 4 2.9950623822697175 6.428753474775224 5 1.6797211149786149 4.50679801299696 6 1.0438363671179482 3.360814809032887 7 0.6504412257144916 2.443244938980349 8 0.4539598149210175 1.9488046425064383 9 0.31932164891229275 1.5421683325792332 >10 1.2664277814788392 11.25587207743936 >50 0.04985685507215618 1.8094702335923567 >100 0.015585483305271505 1.369917599132781 >500 4.127573373351915E-4 0.14394879229708776 >1k 7.504647717317388E-5 0.07909202631056787 >5k 7.504647717317181E-5 0.22012068014946817 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5917 0.11902348043404445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5015 0.10087928923047709 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.011551131215894E-5 0.0 10-11 0.0 0.0 0.0 4.023102262431788E-5 0.0 12-13 0.0 0.0 0.0 4.023102262431788E-5 0.0 14-15 0.0 0.0 0.0 4.023102262431788E-5 0.0 16-17 0.0 0.0 0.0 4.023102262431788E-5 0.0 18-19 0.0 0.0 0.0 5.0288778280397354E-5 0.0 20-21 0.0 0.0 0.0 7.04042895925563E-5 0.0 22-23 0.0 0.0 0.0 8.046204524863576E-5 0.0 24-25 0.0 0.0 0.0 8.046204524863576E-5 0.0 26-27 0.0 0.0 0.0 1.1063531221687418E-4 0.0 28-29 0.0 0.0 0.0 5.230032941161325E-4 0.0 30-31 0.0 0.0 0.0 0.0026552474932049802 0.0 32-33 0.0 0.0 0.0 0.00893128702259857 0.0 34-35 0.0 0.0 0.0 0.0171484733936155 0.0 36-37 0.0 0.0 0.0 0.031842854407147604 0.0 38-39 0.0 0.0 0.0 0.05926029632562024 0.0 40-41 0.0 0.0 0.0 0.09238048570108995 0.0 42-43 0.0 0.0 0.0 0.12579234999058594 0.0 44-45 0.0 0.0 0.0 0.1663753940628666 0.0 46-47 0.0 0.0 0.0 0.21573885882290464 0.0 48-49 0.0 0.0 0.0 0.27095593737478096 0.0 50-51 0.0 0.0 0.0 0.3318556478723421 0.0 52-53 0.0 0.0 0.0 0.3947769672567753 0.0 54-55 0.0 0.0 0.0 0.4607759598719688 0.0 56-57 0.0 0.0 0.0 0.5410670232744512 0.0 58-59 0.0 0.0 0.0 0.6334173357085728 0.0 60-61 0.0 0.0 0.0 0.7324258823870192 0.0 62-63 0.0 0.0 0.0 0.833647135309803 0.0 64-65 0.0 0.0 0.0 0.9440712346578995 0.0 66-67 0.0 0.0 0.0 1.0733837991281132 0.0 68-69 0.0 0.0 0.0 1.218647964068869 0.0 70-71 0.0 0.0 0.0 1.3784958747109402 0.0 72-73 0.0 0.0 0.0 1.5481702126290007 0.0 74-75 0.0 0.0 0.0 1.721948114854742 0.0 76-77 0.0 0.0 0.0 1.9080266522478682 0.0 78-79 0.0 0.0 0.0 2.1233732586001857 0.0 80-81 0.0 0.0 0.0 2.361178833332529 0.0 82-83 0.0 0.0 0.0 2.613708962345372 0.0 84-85 0.0 0.0 0.0 2.8675264840821937 0.0 86-87 0.0 0.0 0.0 3.135404748226214 0.0 88 0.0 0.0 0.0 3.3582041515196868 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5740 0.0 49.76509 1 GTATCAA 10535 0.0 42.730003 1 TCAACGC 12370 0.0 35.448563 4 ATCAACG 12605 0.0 34.86365 3 CAACGCA 12675 0.0 34.631596 5 TATCAAC 13305 0.0 33.36025 2 AACGCAG 13240 0.0 33.224396 6 ACGCAGA 15380 0.0 28.265362 7 CGCAGAG 15605 0.0 27.797588 8 GTACATG 12135 0.0 25.598215 1 TACATGG 12145 0.0 24.441973 2 GCAGAGT 17990 0.0 23.51151 9 ACATGGG 12525 0.0 22.928076 3 AGAGTAC 16545 0.0 20.707607 10-11 GAGTACT 11840 0.0 19.787123 12-13 CAGAGTA 17765 0.0 19.11357 10-11 CATGGGG 9420 0.0 18.909279 4 AGTACTT 12830 0.0 18.864695 12-13 GTGGTAT 3055 0.0 18.515442 1 GTACTTT 13125 0.0 17.635027 14-15 >>END_MODULE