FastQCFastQC Report
Wed 25 May 2016
SRR1033097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410933
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21700.5280666191325594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG558.7027547E-751.3528569
GCTATCC802.4468318E-741.1390763
CGGATTC608.733434E-539.2278759
GCGGATT608.752289E-539.213528
TTAGCGG608.790098E-539.184855
TAGCGGA651.410078E-436.1618166
GTCCTAA1052.0477855E-631.3593431
GCCGTAT1401.900844E-830.1878494
AGCGGAT804.7694327E-429.3814727
GTACATG17250.027.2689931
GCCTTAC700.0087938626.8794381
TCTCGCT1406.3325933E-726.8663372
CGTGCGC901.3123832E-426.142358
CTATCCC1101.0144117E-425.6451384
TACATGG17100.025.0202562
GTATCAA13300.024.7573761
TAAGGTA1151.3707772E-424.5331215
CTCGCTC1401.9222993E-523.5080453
ACACGTG1201.832505E-423.5051777
GGTATCA10250.023.404781
Produced by FastQC (version 0.11.5)