##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033097_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410933
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77645260906279	33.0	31.0	34.0	26.0	34.0
2	30.783052225058587	33.0	31.0	34.0	26.0	34.0
3	30.89804907369328	34.0	31.0	34.0	26.0	34.0
4	34.013556954540036	37.0	35.0	37.0	28.0	37.0
5	34.29031253269998	37.0	35.0	37.0	31.0	37.0
6	34.21395215278403	37.0	35.0	37.0	31.0	37.0
7	34.22133291801826	37.0	35.0	37.0	31.0	37.0
8	34.093852282488875	37.0	35.0	37.0	30.0	37.0
9	35.50319151783867	39.0	37.0	39.0	28.0	39.0
10-11	35.63427249697639	39.0	37.0	39.0	30.0	39.0
12-13	35.59707543565496	39.0	37.0	39.0	30.0	39.0
14-15	36.94491924474306	41.0	38.0	41.0	30.0	41.0
16-17	37.03130924019244	41.0	38.0	41.0	30.0	41.0
18-19	37.083996661256215	41.0	38.0	41.0	30.0	41.0
20-21	37.163351446586184	40.5	38.0	41.0	30.0	41.0
22-23	37.14188079321933	40.0	38.0	41.0	30.0	41.0
24-25	37.1588069101289	40.0	38.0	41.0	30.0	41.0
26-27	36.98550371958446	40.0	38.0	41.0	30.0	41.0
28-29	36.89644905617217	40.0	38.0	41.0	30.0	41.0
30-31	36.90292091411496	40.0	38.0	41.0	30.0	41.0
32-33	36.77813171490243	40.0	38.0	41.0	30.0	41.0
34-35	36.6199672452687	40.0	38.0	41.0	30.0	41.0
36-37	36.45982313418489	40.0	38.0	41.0	29.0	41.0
38-39	36.3024458975064	40.0	38.0	41.0	27.5	41.0
40-41	36.18216959942375	40.0	38.0	41.0	27.0	41.0
42-43	36.02830388408816	40.0	37.0	41.0	27.0	41.0
44-45	35.897687700914744	40.0	37.0	41.0	26.5	41.0
46-47	35.75793620857901	40.0	37.0	41.0	26.0	41.0
48-49	35.63010515096135	40.0	37.0	41.0	26.0	41.0
50-51	35.50413692743099	40.0	36.0	41.0	25.0	41.0
52-53	35.36333173534372	40.0	36.0	41.0	24.5	41.0
54-55	35.21616540895961	40.0	35.0	41.0	24.0	41.0
56-57	35.04763306913779	40.0	35.0	41.0	23.5	41.0
58-59	34.853533301049076	39.5	35.0	41.0	22.5	41.0
60-61	34.64625620234929	39.0	35.0	41.0	22.0	41.0
62-63	34.39788603008276	39.0	35.0	41.0	20.5	41.0
64-65	34.1185850248094	38.5	34.5	41.0	18.5	41.0
66-67	33.833734453061695	38.0	34.0	40.0	16.5	41.0
68-69	33.514963509866575	37.0	34.0	40.0	12.0	41.0
70-71	33.16772442222941	37.0	34.0	40.0	7.5	41.0
72-73	32.81017830157228	36.0	34.0	39.0	4.5	41.0
74-75	32.4576646314606	36.0	33.5	39.0	2.0	41.0
76-77	32.078712344834805	35.0	33.0	38.5	2.0	40.0
78-79	31.692727281576317	35.0	33.0	37.0	2.0	39.0
80-81	31.345480893478985	35.0	33.0	37.0	2.0	39.0
82-83	31.122080728488587	35.0	33.0	36.5	2.0	39.0
84-85	30.896272628384676	35.0	33.0	36.0	2.0	37.0
86-87	30.727349713943635	35.0	33.0	36.0	2.0	37.0
88-89	30.509828852878695	35.0	33.0	35.0	2.0	37.0
90-91	30.291322429690485	35.0	32.5	35.0	2.0	36.0
92-93	30.0694675287699	35.0	32.0	35.0	2.0	36.0
94-95	29.874595128646277	35.0	32.0	35.0	2.0	36.0
96-97	29.706219748718162	35.0	32.0	35.0	2.0	35.5
98-99	29.530015841998576	35.0	32.0	35.0	2.0	35.0
100	29.394969496243913	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2384598087414922
1101	2	0.3304060675488998
1101	3	0.3037083162630694
1101	4	0.36363110646794894
1101	5	0.08558309911007456
1101	6	0.31864552753349074
1101	7	0.43128094713026144
1101	8	0.4488731991260053
1101	9	0.7144235035389386
1101	10-11	0.43572775174443734
1101	12-13	0.3210192774025842
1101	14-15	0.6174806190793163
1101	16-17	0.49972845486670536
1101	18-19	0.4521953969240897
1101	20-21	0.5817287435104745
1101	22-23	0.5081568604431439
1101	24-25	0.6237171025931332
1101	26-27	0.7287176590518669
1101	28-29	0.7909327915368181
1101	30-31	0.7096466850077121
1101	32-33	0.6248539300882214
1101	34-35	0.5411734591267887
1101	36-37	0.6041137199970876
1101	38-39	0.8221539718731421
1101	40-41	0.8094710439653596
1101	42-43	0.6654940624009598
1101	44-45	0.7834504886824902
1101	46-47	0.594171525549001
1101	48-49	0.704614177948784
1101	50-51	0.661328373790532
1101	52-53	0.6056788404324749
1101	54-55	0.8193080986581833
1101	56-57	0.6725373994315618
1101	58-59	0.7879708678493316
1101	60-61	0.7888067326833408
1101	62-63	0.7363978187389719
1101	64-65	0.7489886838396913
1101	66-67	0.6715041229758043
1101	68-69	0.7239756849330163
1101	70-71	0.7971860589691673
1101	72-73	0.6632595411178528
1101	74-75	0.6396474701186889
1101	76-77	0.5861399949333332
1101	78-79	0.6349479580048332
1101	80-81	0.6311101330272137
1101	82-83	0.8514105016667379
1101	84-85	0.7774864698676005
1101	86-87	0.7303667801125933
1101	88-89	0.5032192652300793
1101	90-91	0.5279026089355625
1101	92-93	0.6718417784408679
1101	94-95	0.7132607808031963
1101	96-97	0.4507071846186079
1101	98-99	0.5664463205871151
1101	100	0.669609308680883
1102	1	0.08378310840203795
1102	2	-0.05475200369562927
1102	3	-0.01088822375574594
1102	4	0.16017112528108868
1102	5	-0.06952303151852846
1102	6	0.20082212562763857
1102	7	0.1789845175363851
1102	8	0.25085266826837227
1102	9	0.019102248784527376
1102	10-11	-0.015515551187959886
1102	12-13	0.033206917989879514
1102	14-15	0.3042746152348883
1102	16-17	0.037665553553878794
1102	18-19	-0.048908852390866286
1102	20-21	0.245501186355348
1102	22-23	0.06518520284222973
1102	24-25	0.13754680280988651
1102	26-27	-0.056839168889737834
1102	28-29	-0.04255459139230311
1102	30-31	0.11492254284583936
1102	32-33	0.07596840393386373
1102	34-35	0.0159270479370619
1102	36-37	-0.13794999927819873
1102	38-39	-0.034185237157160486
1102	40-41	-0.09053100094078559
1102	42-43	0.1255754496652841
1102	44-45	-0.030906797318081658
1102	46-47	-0.1821770900495423
1102	48-49	-0.18911613982962905
1102	50-51	-0.2338967703753454
1102	52-53	-0.12478208141121172
1102	54-55	-0.1208579477202889
1102	56-57	-0.18783027469220315
1102	58-59	-0.29559422151306336
1102	60-61	-0.23696930919633985
1102	62-63	0.0678648344029611
1102	64-65	0.045142215392594665
1102	66-67	-0.06162335647288586
1102	68-69	0.09553922016674932
1102	70-71	-0.10995884307983061
1102	72-73	-0.06434260194377828
1102	74-75	0.07915710162659906
1102	76-77	0.10663608916260614
1102	78-79	0.4601330220103925
1102	80-81	0.38571117183953163
1102	82-83	0.35844293387809856
1102	84-85	0.3156505531361802
1102	86-87	0.18713337452591006
1102	88-89	0.2094194206429485
1102	90-91	0.19826373935967823
1102	92-93	0.35896295957865476
1102	94-95	0.2025393379174254
1102	96-97	0.05709956120852411
1102	98-99	0.16937462617788768
1102	100	0.08055773614806228
1103	1	0.37249179991634307
1103	2	0.33095763952904633
1103	3	0.37673644643405524
1103	4	0.261369661349363
1103	5	0.19837956904940057
1103	6	0.648926829243365
1103	7	0.45957658971162374
1103	8	0.4798163872120469
1103	9	0.5925812531252603
1103	10-11	0.4818943264824469
1103	12-13	0.5068162989138898
1103	14-15	0.612737100050083
1103	16-17	0.5329882452693582
1103	18-19	0.5241755403328057
1103	20-21	0.468628908982069
1103	22-23	0.5461877484740327
1103	24-25	0.6853276927751537
1103	26-27	0.36539719573139706
1103	28-29	0.38274194763168623
1103	30-31	0.3516488912956319
1103	32-33	0.25494218160503834
1103	34-35	0.3057073808035753
1103	36-37	0.3464744480721009
1103	38-39	0.47835915664975914
1103	40-41	0.6013353572582361
1103	42-43	0.1983677524175036
1103	44-45	0.23364353887554046
1103	46-47	0.23399502251535864
1103	48-49	0.1470852204198252
1103	50-51	0.11916069590856893
1103	52-53	0.16069814545178218
1103	54-55	0.47278300213309166
1103	56-57	0.39603436578566686
1103	58-59	0.09919535764525023
1103	60-61	0.07824412926359514
1103	62-63	0.12931011879413035
1103	64-65	-0.08609129409742877
1103	66-67	-0.038975744646919
1103	68-69	0.0423981890698073
1103	70-71	-0.07446314125146358
1103	72-73	-0.035058164342714804
1103	74-75	0.07847813752078281
1103	76-77	0.04074562096752743
1103	78-79	0.10781061658177649
1103	80-81	0.2645905522219181
1103	82-83	0.029071836470929924
1103	84-85	-0.04314232218976244
1103	86-87	0.042666835269788095
1103	88-89	0.06425070483295059
1103	90-91	-0.010431643684402303
1103	92-93	-0.12964746590552068
1103	94-95	0.029476997019415307
1103	96-97	0.08305688125402355
1103	98-99	0.19088095930746718
1103	100	-0.05223294173279314
1104	1	0.21805164547618716
1104	2	0.21630588202014422
1104	3	0.5118715815691885
1104	4	0.4003658003455044
1104	5	0.3091452512436561
1104	6	0.632003598034423
1104	7	0.6240453256089253
1104	8	0.5041607686992862
1104	9	0.5442936334090689
1104	10-11	0.6098464901488896
1104	12-13	0.614711299666034
1104	14-15	0.6354769085041738
1104	16-17	0.7481514604325739
1104	18-19	0.5755720203007115
1104	20-21	0.46132058024516454
1104	22-23	0.3654852463429563
1104	24-25	0.39616608218496907
1104	26-27	0.5759347276975006
1104	28-29	0.3597639603679852
1104	30-31	0.177364681297135
1104	32-33	0.15509511747226412
1104	34-35	0.3905241084774431
1104	36-37	0.4013307886427242
1104	38-39	0.5398719681625721
1104	40-41	0.626632453983909
1104	42-43	0.7574235614733098
1104	44-45	0.7265859246750708
1104	46-47	0.6591065904840647
1104	48-49	0.5740019330508233
1104	50-51	0.6903636242543527
1104	52-53	0.6539163172413822
1104	54-55	0.7645771153557774
1104	56-57	0.7956357296727532
1104	58-59	0.8524421108920066
1104	60-61	0.8792520017000385
1104	62-63	0.908568505195376
1104	64-65	0.7893411884779056
1104	66-67	0.9826358483932438
1104	68-69	0.8685025866027729
1104	70-71	0.6916201962604518
1104	72-73	0.7339459975185889
1104	74-75	0.8994804942374302
1104	76-77	0.9198134643210878
1104	78-79	0.8666733754445382
1104	80-81	1.0221119883147836
1104	82-83	1.0954735814441001
1104	84-85	0.9590263585503465
1104	86-87	0.7889938673111097
1104	88-89	0.8412526604063331
1104	90-91	0.9332829428873239
1104	92-93	0.9950328730605342
1104	94-95	1.1625186657753694
1104	96-97	1.0603546799803922
1104	98-99	1.2534593076000995
1104	100	1.2074571751001812
1105	1	-0.5258080036466204
1105	2	-0.4390890989387479
1105	3	-0.40968230314258847
1105	4	-0.728205628225929
1105	5	-0.5410907283156305
1105	6	-0.5368145512348548
1105	7	-0.515049163861498
1105	8	-0.413890853863613
1105	9	-0.4232420890138755
1105	10-11	-0.3317445003142865
1105	12-13	-0.3792671529590379
1105	14-15	-0.5571165882246589
1105	16-17	-0.5591508863434598
1105	18-19	-0.20902909287182325
1105	20-21	-0.18712195466353165
1105	22-23	-0.2939734653713941
1105	24-25	-0.3133040216460401
1105	26-27	-0.7330617895696889
1105	28-29	-0.6379787730890314
1105	30-31	-0.37941525555798705
1105	32-33	-0.5551675521888981
1105	34-35	-0.5697108946147083
1105	36-37	-0.603816820640219
1105	38-39	-0.5421854481053714
1105	40-41	-0.5552622177603865
1105	42-43	-0.5442624717916686
1105	44-45	-0.5298327193375627
1105	46-47	-0.4439058106486371
1105	48-49	-0.6565387042567323
1105	50-51	-0.5856283036172698
1105	52-53	-0.6276544929008594
1105	54-55	-0.7205415254019627
1105	56-57	-0.6917662167517591
1105	58-59	-0.7464186774424704
1105	60-61	-0.7941327589027694
1105	62-63	-0.9367672367569071
1105	64-65	-0.8555046924581973
1105	66-67	-0.6608446059894604
1105	68-69	-0.6672881890376274
1105	70-71	-0.7343391827064494
1105	72-73	-0.6387633733110789
1105	74-75	-0.7998799197846438
1105	76-77	-0.9248338682961688
1105	78-79	-0.726867294411921
1105	80-81	-0.5813316858055835
1105	82-83	-0.6914287392928067
1105	84-85	-0.646000819785101
1105	86-87	-0.6277964851935316
1105	88-89	-0.6272855682822716
1105	90-91	-0.596694598368412
1105	92-93	-0.6471879387091519
1105	94-95	-0.5113006227055763
1105	96-97	-0.4106605203581175
1105	98-99	-0.6258737653419963
1105	100	-0.6309635520423527
1106	1	-0.42568733159612293
1106	2	-0.3824939072558351
1106	3	-0.3699626335983588
1106	4	-0.5308511308714259
1106	5	-0.3201866161834488
1106	6	-0.6073055936557239
1106	7	-0.4619163317813033
1106	8	-0.7172139764849774
1106	9	-0.7085293813187121
1106	10-11	-0.5523422910875126
1106	12-13	-0.5051837964195371
1106	14-15	-0.4535196328733164
1106	16-17	-0.546782001167557
1106	18-19	-0.5005634844062214
1106	20-21	-0.4614559377694505
1106	22-23	-0.22323643761681922
1106	24-25	-0.4243176668702091
1106	26-27	-0.38953630920210713
1106	28-29	-0.4724584909020848
1106	30-31	-0.40118727820720324
1106	32-33	-0.34924533924914414
1106	34-35	-0.470592728829871
1106	36-37	-0.3936942920966402
1106	38-39	-0.5186497570258197
1106	40-41	-0.5580144687582518
1106	42-43	-0.6350728310230807
1106	44-45	-0.7076429857443145
1106	46-47	-0.6149491597621619
1106	48-49	-0.5417703017690343
1106	50-51	-0.5850435082956338
1106	52-53	-0.6862082847879805
1106	54-55	-0.6401275274441076
1106	56-57	-0.8528216330703273
1106	58-59	-0.6838734254235348
1106	60-61	-0.7364515187653922
1106	62-63	-0.7162854768014668
1106	64-65	-0.46410025543972466
1106	66-67	-0.5248425638470735
1106	68-69	-0.6352079885363722
1106	70-71	-0.7202994537544427
1106	72-73	-0.6782370575215992
1106	74-75	-0.6568881811788643
1106	76-77	-0.5305518669966247
1106	78-79	-0.539050530279372
1106	80-81	-0.7368036991723343
1106	82-83	-0.6158230584239668
1106	84-85	-0.5324530615005045
1106	86-87	-0.45857249930287836
1106	88-89	-0.2766497194008082
1106	90-91	-0.3333974666502293
1106	92-93	-0.4013000708914589
1106	94-95	-0.32210077122502057
1106	96-97	-0.3514848032876614
1106	98-99	-0.32992556149905283
1106	100	-0.18226773843425903
1107	1	0.11234694748960905
1107	2	0.2547999861901822
1107	3	0.3500786764876942
1107	4	0.24192859996199445
1107	5	0.35400523306432774
1107	6	0.5259253522150473
1107	7	0.5010111210790384
1107	8	0.20444777795082558
1107	9	0.35847226398271204
1107	10-11	0.48372117730258424
1107	12-13	0.33676369597537814
1107	14-15	0.5582490069713799
1107	16-17	0.6545839541133844
1107	18-19	0.4773803031013273
1107	20-21	0.48722456600054187
1107	22-23	0.44327191317564996
1107	24-25	0.3450087064830072
1107	26-27	0.12641660440181113
1107	28-29	0.1768422559937477
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2216	40-41	-0.40032330281626116
2216	42-43	-0.44216068266538855
2216	44-45	-0.231723171540672
2216	46-47	-0.2987810350131781
2216	48-49	-0.24600659841456718
2216	50-51	-0.15215021896670322
2216	52-53	-0.1583475622077941
2216	54-55	-0.12505776649975786
2216	56-57	-0.10982828611658135
2216	58-59	-0.12192675456596902
2216	60-61	-0.14088164431763062
2216	62-63	-0.30308939528854495
2216	64-65	-0.17307750237076647
2216	66-67	-0.10780482832748817
2216	68-69	-0.165539593125402
2216	70-71	0.08299913007220283
2216	72-73	-0.030822316047455445
2216	74-75	-0.12784876930117406
2216	76-77	-0.0821959202908431
2216	78-79	-0.28309160105311193
2216	80-81	-7.51522526098114E-4
2216	82-83	-0.30268460172115397
2216	84-85	-0.1268791707780963
2216	86-87	-0.23159186473148452
2216	88-89	-0.2747987406521375
2216	90-91	-0.2701935272086651
2216	92-93	-0.35211816857423983
2216	94-95	-0.37788333360824566
2216	96-97	-0.387500309600199
2216	98-99	-0.48527681090970987
2216	100	-0.4223711138511632
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9359.0
3	1408.0
4	808.0
5	921.0
6	1905.0
7	3887.0
8	4384.0
9	2888.0
10	1812.0
11	1792.0
12	1748.0
13	1329.0
14	1236.0
15	1279.0
16	1260.0
17	1194.0
18	1106.0
19	952.0
20	876.0
21	796.0
22	1118.0
23	1755.0
24	2873.0
25	3098.0
26	3100.0
27	2551.0
28	2655.0
29	3299.0
30	3865.0
31	4941.0
32	6349.0
33	8680.0
34	12324.0
35	19450.0
36	33817.0
37	72747.0
38	129347.0
39	57935.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47742790077042	21.678955109037606	13.616795394999562	22.226821595192412
2	15.82878301365461	23.63983377842479	38.22122465473968	22.310158553180923
3	17.88409847879862	28.11203521551976	30.620264505950413	23.38360179973121
4	12.127919661815985	19.983733660608184	37.913233202813004	29.97511347476282
5	12.083603259572437	40.68376984262285	34.408898067734036	12.823728830070676
6	27.905991232342913	40.79566488066245	17.48222113979542	13.81612274719922
7	24.186331984831178	36.86403834584818	22.138979343804724	16.81065032551592
8	23.090263822614553	37.650807105745834	21.07804092648481	18.180888145154807
9	23.576821901792673	19.75910950896337	21.383476227591583	35.28059236165238
10-11	21.56964954480407	30.475778704746826	28.272934871966633	19.68163687848248
12-13	22.31446228347742	29.2722941223756	27.800634799999024	20.612608794147953
14-15	20.699155561564346	30.8450839749141	25.4855246955308	22.970235767990754
16-17	19.36716829363625	33.53391918038236	26.423146843695243	20.675765682286144
18-19	19.597999741807882	32.18608344477003	28.320630768218987	19.895286045203108
20-21	21.695110100077695	31.217591656701117	27.36000935260034	19.727288890620855
22-23	21.07381373821286	31.1749037672592	27.281647241571573	20.469635252956373
24-25	21.18427073496052	31.30080905046099	26.967375113179443	20.547545101399045
26-27	20.39542321155215	31.02044149335517	27.473771706594125	21.11036358849855
28-29	20.379909734304253	31.237482544046223	27.77380736419055	20.60880035745897
30-31	21.139833913742297	32.04161897572024	26.850936366071647	19.967610744465823
32-33	20.336426066627702	31.65047243327489	27.623222287161504	20.389879212935906
34-35	20.478168327615645	31.440674975265264	27.460969017389157	20.62018767972993
36-37	20.838937979843966	31.63966495068114	26.98641859740399	20.534978472070904
38-39	20.69256479097036	31.378493720283213	27.531033307155163	20.39790818159127
40-41	20.33027782107232	31.157457917705734	27.91751091022444	20.594753350997504
42-43	20.193888211099992	30.875638318856254	27.94807731176747	20.98239615827629
44-45	20.438172164111233	31.18293578272076	27.775863517431066	20.603028535736943
46-47	20.349138781265296	31.498591220025375	27.43650749198201	20.71576250672732
48-49	20.181663216851334	30.893826859856325	27.596128089614027	21.328381833678314
50-51	20.240047629657496	30.468857484808304	27.272970778133704	22.018124107400496
52-53	20.217093527982904	30.237853172694674	27.493075045202453	22.051978254119966
54-55	20.43195860599604	30.78557297215036	27.250159793029983	21.532308628823618
56-57	20.623238429181047	30.083703149064654	27.123716298068434	22.169342123685862
58-59	20.34013241034057	29.752759360398155	27.562071912596515	22.34503631666476
60-61	20.816608777485186	29.812502358786315	27.041248020141296	22.329640843587207
62-63	20.652072013061407	29.70009167822283	27.118734864875066	22.529101443840695
64-65	20.66699781587617	29.389005359260366	27.413919467434155	22.53007735742931
66-67	20.784593507416897	29.38901929464016	27.26670627538448	22.559680922558464
68-69	21.702574241996277	29.750367679286267	26.819038482141988	21.728019596575468
70-71	21.561005658411165	29.28069517622883	27.036808889841158	22.121490275518852
72-73	21.33579891921635	29.311904324230227	27.417793852510407	21.93450290404302
74-75	21.63295666761624	29.384917301544398	27.29450779018721	21.68761824065214
76-77	21.713873208831583	29.111911581925376	27.39801402622888	21.776201183014166
78-79	22.18662316873505	28.74990109495493	27.74332162703364	21.32015410927638
80-81	22.36655837397859	28.548979415462927	27.528455739776202	21.556006470782275
82-83	22.04898665715548	28.349983992481942	27.974446401595156	21.62658294876742
84-85	21.943055508220827	28.26130880799248	27.825193653143	21.970442030643692
86-87	22.22636057464675	28.336516213212786	27.609991930212775	21.82713128192769
88-89	22.00224936280226	28.114357215366752	27.687732939289113	22.195660482541875
90-91	22.2759659880671	27.76992042298264	27.594773087437346	22.35934050151291
92-93	22.320936261890417	28.265688046594	27.366306987274285	22.047068704241298
94-95	21.718793361149384	28.153892218484206	27.648894579765038	22.478419840601372
96-97	21.536839875896	28.5392949344486	27.50493263499475	22.418932554660657
98-99	21.43057110862262	28.72437801256147	27.256560689420127	22.58849018939578
100	21.49009621377376	29.30819569959489	26.923788563415396	22.277919523215957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1567.0
1	1040.0
2	460.0
3	348.0
4	257.0
5	201.5
6	166.0
7	163.5
8	210.0
9	229.5
10	156.0
11	97.5
12	95.0
13	100.5
14	103.5
15	105.5
16	124.5
17	159.5
18	200.5
19	232.0
20	309.5
21	439.5
22	634.0
23	1035.0
24	1488.5
25	1925.5
26	2675.5
27	3769.0
28	5343.0
29	6816.5
30	7687.5
31	8780.5
32	10234.0
33	11869.0
34	13481.5
35	14670.5
36	15304.0
37	15586.0
38	15855.5
39	16005.0
40	15931.5
41	15416.5
42	14577.5
43	13986.5
44	13689.5
45	13347.0
46	13060.0
47	12902.5
48	12764.5
49	12480.5
50	12284.0
51	11871.0
52	10866.5
53	10263.0
54	9756.0
55	9001.5
56	8230.5
57	7246.0
58	6268.5
59	5382.0
60	4388.0
61	3437.0
62	2790.0
63	2248.0
64	1749.5
65	1349.0
66	1023.0
67	776.0
68	646.5
69	564.5
70	444.0
71	363.0
72	403.5
73	402.5
74	290.5
75	286.0
76	331.5
77	220.5
78	126.0
79	165.0
80	143.0
81	116.5
82	114.5
83	53.5
84	9.0
85	6.0
86	3.5
87	2.5
88	2.0
89	2.0
90	0.5
91	0.0
92	0.0
93	1.0
94	1.0
95	1.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060593819430418104
2	0.037962392896165556
3	0.04988647784432012
4	0.06546079287864445
5	0.07957501587850087
6	0.08103510791296878
7	0.08614543003360645
8	0.09539267958523652
9	0.09076905480942148
10-11	0.09624439993867615
12-13	0.09977295568864024
14-15	0.10305816276619303
16-17	0.07361297340442359
18-19	0.09393258755076862
20-21	0.08565873268878382
22-23	0.051103221206376705
24-25	0.01971124246531673
26-27	0.16766723529139788
28-29	0.06241893447350298
30-31	0.07373464774062925
32-33	0.07130116101651607
34-35	0.01788612742223185
36-37	0.04562787607712206
38-39	0.0694760459734312
40-41	0.07519473977509716
42-43	0.06911102296481422
44-45	0.06631251323208406
46-47	0.0715445096889274
48-49	0.0688676742924029
50-51	0.06436572385279352
52-53	0.0682593026113746
54-55	0.059742099076978486
56-57	0.062175585801091664
58-59	0.05840368137871624
60-61	0.05706526368045399
62-63	0.0626622831459143
64-65	0.058890378723538875
66-67	0.060593819430418104
68-69	0.060593819430418104
70-71	0.052806661913255934
72-73	0.051711592887405
74-75	0.05451010262013515
76-77	0.04927810616329183
78-79	0.045141178732299427
80-81	0.039787507939250434
82-83	0.04587122474953338
84-85	0.0357722548444637
86-87	0.03443383714620145
88-89	0.03650230086169765
90-91	0.0330954194479392
92-93	0.03662397519790331
94-95	0.033825465465173156
96-97	0.03613727785308067
98-99	0.0372323468789316
100	0.0440461097064485
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	410933.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.73521137819841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98953213166766	71.84588687097168
2	8.478366450969476	13.690054151216932
3	1.414725612576079	3.426545141204429
4	0.40995296330546793	1.323905565903431
5	0.15911566432501625	0.6423118396431325
6	0.09117682621977952	0.44167082025882975
7	0.05534507422406428	0.3127807386355337
8	0.057511008715302	0.3714530756162655
9	0.04024512506027335	0.2924278810814879
>10	0.2636475173580763	4.079617846885878
>50	0.02742334288225472	1.500430677641183
>100	0.012656927285084572	1.5449545460653962
>500	0.0	0.0
>1k	3.013554115496324E-4	0.5279608448758909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2170	0.5280666191325594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.4334867241131765E-4	0.0
14-15	0.0	0.0	0.0	2.4334867241131765E-4	0.0
16-17	0.0	0.0	0.0	6.083716810282942E-4	0.0
18-19	0.0	0.0	0.0	9.733946896452706E-4	0.0
20-21	0.0	0.0	0.0	0.0013384176982622472	0.0
22-23	0.0	0.0	0.0	0.002433486724113177	0.0
24-25	0.0	0.0	0.0	0.005718693801665965	0.0
26-27	0.0	0.0	0.0	0.0124107822929772	0.0
28-29	0.0	0.0	0.0	0.0289584920169468	0.0
30-31	0.0	0.0	0.0	0.06911102296481421	0.0
32-33	0.0	0.0	0.0	0.14150725300718123	0.0
34-35	0.0	0.0	0.0	0.2668318192990098	0.0
36-37	0.0	0.0	0.0	0.44435467582306604	0.0
38-39	0.0	0.0	0.0	0.6858782331912988	0.0
40-41	0.0	0.0	0.0	0.9525883781541029	0.0
42-43	0.0	0.0	0.0	1.1807277585397133	0.0
44-45	0.0	0.0	0.0	1.4339320521836894	0.0
46-47	0.0	0.0	0.0	1.7426198431374458	0.0
48-49	0.0	0.0	0.0	2.04717070666021	0.0
50-51	0.0	0.0	0.0	2.3529383135450304	0.0
52-53	0.0	0.0	0.0	2.652987226628185	0.0
54-55	0.0	0.0	0.0	2.983333049426549	0.0
56-57	0.0	0.0	0.0	3.3792613394397626	0.0
58-59	0.0	0.0	0.0	3.792102362185563	0.0
60-61	0.0	0.0	0.0	4.221612768991539	0.0
62-63	0.0	0.0	0.0	4.620339568737483	0.0
64-65	0.0	0.0	0.0	5.046321419793495	0.0
66-67	0.0	0.0	0.0	5.544821175228078	0.0
68-69	0.0	0.0	0.0	6.127276222644568	0.0
70-71	0.0	0.0	0.0	6.736377949690095	0.0
72-73	0.0	0.0	0.0	7.347426466114914	0.0
74-75	0.0	0.0	0.0	7.9761177612895535	0.0
76-77	0.0	0.0	0.0	8.654938882980925	0.0
78-79	0.0	0.0	0.0	9.40871139577498	0.0
80-81	0.0	0.0	0.0	10.19351086430148	0.0
82-83	0.0	0.0	0.0	11.018341189439642	0.0
84-85	0.0	0.0	0.0	11.83903458714681	0.0
86-87	0.0	1.2167433620565883E-4	0.0	12.704504140577662	0.0
88	0.0	2.4334867241131765E-4	0.0	13.393667580846513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGCG	55	8.7027547E-7	51.352856	9
GCTATCC	80	2.4468318E-7	41.139076	3
CGGATTC	60	8.733434E-5	39.227875	9
GCGGATT	60	8.752289E-5	39.21352	8
TTAGCGG	60	8.790098E-5	39.18485	5
TAGCGGA	65	1.410078E-4	36.161816	6
GTCCTAA	105	2.0477855E-6	31.359343	1
GCCGTAT	140	1.900844E-8	30.18784	94
AGCGGAT	80	4.7694327E-4	29.381472	7
GTACATG	1725	0.0	27.268993	1
GCCTTAC	70	0.00879386	26.879438	1
TCTCGCT	140	6.3325933E-7	26.866337	2
CGTGCGC	90	1.3123832E-4	26.14235	8
CTATCCC	110	1.0144117E-4	25.645138	4
TACATGG	1710	0.0	25.020256	2
GTATCAA	1330	0.0	24.757376	1
TAAGGTA	115	1.3707772E-4	24.533121	5
CTCGCTC	140	1.9222993E-5	23.508045	3
ACACGTG	120	1.832505E-4	23.505177	7
GGTATCA	1025	0.0	23.40478	1
>>END_MODULE