FastQCFastQC Report
Wed 25 May 2016
SRR1033097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410933
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.5925540173215585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.584523511131985No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT16950.4124759997371834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.29737207768663015No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT6970.16961402467068842RNA PCR Primer, Index 23 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.15890668308459044No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT4640.1129137839988514RNA PCR Primer, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG452.2005406E-762.6630179
GGTATCA15100.051.5113261
GTATCAA27100.038.6170771
GTACACG753.2730267E-431.3315054
ATCAACG33200.031.288133
TATCAAC33750.031.1249682
TCAACGC33600.030.9118884
CAACGCA34500.030.2417165
CGTGCGC552.9684343E-629.9073510-11
TACTAGG650.006082196728.9884132
AACGCAG36300.028.7421286
ATAGGAC856.8060576E-427.6488133
CGCAGAG40350.025.7407598
CGTGCTC551.0183934E-425.63487218-19
ACGCAGA41450.025.0576467
GTACTAT950.001280653824.8108771
GCAGAGT42650.024.2424329
ATCTAAG1601.9813142E-623.5703351
GTCCTAA1401.8833342E-523.5703351
CGCTCGT601.8381506E-423.4986314-15
Produced by FastQC (version 0.11.5)