##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033097_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 410933 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.600492051015614 34.0 31.0 34.0 31.0 34.0 2 32.59344223997586 34.0 31.0 34.0 31.0 34.0 3 32.667692786902 34.0 31.0 34.0 31.0 34.0 4 36.208087449778915 37.0 35.0 37.0 35.0 37.0 5 36.20405029530362 37.0 35.0 37.0 35.0 37.0 6 36.29359773977753 37.0 37.0 37.0 35.0 37.0 7 36.23040252303903 37.0 37.0 37.0 35.0 37.0 8 36.24769487970058 37.0 37.0 37.0 35.0 37.0 9 38.025899599204735 39.0 39.0 39.0 35.0 39.0 10-11 37.99517439582608 39.0 38.5 39.0 35.0 39.0 12-13 37.969950089187286 39.0 38.0 39.0 35.0 39.0 14-15 39.37190125884268 41.0 39.0 41.0 36.0 41.0 16-17 39.38123368042966 41.0 39.0 41.0 36.0 41.0 18-19 39.45778022208 41.0 39.0 41.0 36.0 41.0 20-21 39.47321217814097 41.0 39.0 41.0 36.0 41.0 22-23 39.365512139448526 41.0 39.0 41.0 36.0 41.0 24-25 39.28485787220788 41.0 39.0 41.0 36.0 41.0 26-27 39.108105214232005 41.0 39.0 41.0 35.0 41.0 28-29 38.99478138772014 41.0 39.0 41.0 35.0 41.0 30-31 38.82563945947393 40.5 38.5 41.0 35.0 41.0 32-33 38.578853487064805 41.0 38.0 41.0 35.0 41.0 34-35 38.36885331672073 41.0 38.0 41.0 35.0 41.0 36-37 38.162805128816615 40.5 38.0 41.0 34.0 41.0 38-39 37.92497925452568 40.0 38.0 41.0 34.0 41.0 40-41 37.71157463625457 40.0 38.0 41.0 33.0 41.0 42-43 37.44173259387783 40.0 38.0 41.0 33.0 41.0 44-45 37.04383317961809 40.0 37.0 41.0 32.5 41.0 46-47 36.524783845541734 40.0 37.0 41.0 30.5 41.0 48-49 36.06655099493105 40.0 37.0 41.0 28.0 41.0 50-51 35.79045975864679 40.0 37.0 41.0 26.0 41.0 52-53 35.632656661791586 40.0 36.0 41.0 24.5 41.0 54-55 35.47308685357467 40.0 36.0 41.0 24.0 41.0 56-57 35.306792104795676 40.0 35.5 41.0 23.5 41.0 58-59 35.12396424721305 40.0 35.0 41.0 23.0 41.0 60-61 34.924356281924304 39.0 35.0 41.0 22.5 41.0 62-63 34.67699722339165 39.0 35.0 41.0 21.5 41.0 64-65 34.39138132006921 39.0 35.0 41.0 19.5 41.0 66-67 34.06691358445294 38.0 35.0 41.0 16.5 41.0 68-69 33.71053675416674 37.0 35.0 40.0 10.0 41.0 70-71 33.299770278853245 37.0 34.0 40.0 4.5 41.0 72-73 32.91296513056873 36.0 34.0 39.0 2.0 41.0 74-75 32.56683692962113 36.0 34.0 39.0 2.0 41.0 76-77 32.213149102165076 35.0 34.0 38.5 2.0 40.0 78-79 31.84190853496799 35.0 34.0 37.0 2.0 39.0 80-81 31.51492579082235 35.0 33.0 37.0 2.0 39.0 82-83 31.20880046138908 35.0 33.0 36.5 2.0 39.0 84-85 30.993003725668174 35.0 33.0 36.0 2.0 37.0 86-87 30.7912482083454 35.0 33.0 36.0 2.0 37.0 88-89 30.57831933672886 35.0 33.0 35.0 2.0 37.0 90-91 30.3842305193304 35.0 33.0 35.0 2.0 36.0 92-93 30.208104484185988 35.0 33.0 35.0 2.0 36.0 94-95 30.052114334940246 35.0 33.0 35.0 2.0 36.0 96-97 29.907245220023704 35.0 33.0 35.0 2.0 35.5 98-99 29.769857860040446 35.0 33.0 35.0 2.0 35.0 100 29.653160004185597 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1123224260356892 1101 2 0.15698913636422418 1101 3 0.11051165300893473 1101 4 0.22097524435105242 1101 5 0.2561968085206985 1101 6 0.18400125045342008 1101 7 0.20181753082932374 1101 8 0.2621762965124077 1101 9 0.21866997791131837 1101 10-11 0.2695003882916822 1101 12-13 0.3073623261917007 1101 14-15 0.35021707453408624 1101 16-17 0.3726290689583962 1101 18-19 0.24710581978617796 1101 20-21 0.26994679855103954 1101 22-23 0.39573156187949365 1101 24-25 0.514773575187931 1101 26-27 0.39267798509738583 1101 28-29 0.5329692597770972 1101 30-31 0.4779488683834927 1101 32-33 0.5017653447499839 1101 34-35 0.6603893523868436 1101 36-37 0.7848684239819192 1101 38-39 0.5515877675233298 1101 40-41 0.7396193424662982 1101 42-43 0.9986585803283248 1101 44-45 0.9094053401832198 1101 46-47 1.0255271063940796 1101 48-49 1.0050267631359304 1101 50-51 1.1478267492021104 1101 52-53 1.2192004191522443 1101 54-55 1.1663222646367046 1101 56-57 1.2739397523733516 1101 58-59 1.170449067696289 1101 60-61 1.2549942702152919 1101 62-63 1.0716388778706545 1101 64-65 1.169880220787519 1101 66-67 1.3279128411725694 1101 68-69 1.167772611119183 1101 70-71 1.1233841259590918 1101 72-73 1.1955436559542818 1101 74-75 0.9754196981268777 1101 76-77 0.9081139362014454 1101 78-79 0.8079497415027888 1101 80-81 0.8457297494588012 1101 82-83 0.8636847577371292 1101 84-85 0.9120765389437082 1101 86-87 0.7988583898196637 1101 88-89 1.00445932366301 1101 90-91 0.9669978036253966 1101 92-93 0.8150701611949955 1101 94-95 0.8414443442747199 1101 96-97 0.8580082782023339 1101 98-99 1.0141760232765655 1101 100 1.1258513129027534 1102 1 0.18294530444557466 1102 2 0.14292836175377488 1102 3 0.18429595630941265 1102 4 0.04249992636814426 1102 5 0.07731659912231237 1102 6 0.197027302557629 1102 7 0.11953459761596008 1102 8 0.13210799664737038 1102 9 0.15313891578707484 1102 10-11 0.12404825954438792 1102 12-13 0.1664601136032644 1102 14-15 0.028835535946711843 1102 16-17 0.14393699092477874 1102 18-19 0.10153508648283349 1102 20-21 0.07446808602394128 1102 22-23 0.10896005969144085 1102 24-25 0.18824296294303622 1102 26-27 0.0964794656094341 1102 28-29 0.09721612084616993 1102 30-31 -0.07829831987056934 1102 32-33 0.09782685552664105 1102 34-35 0.2044120917431016 1102 36-37 0.17570519957392605 1102 38-39 0.1331079507469255 1102 40-41 -0.07150699183495135 1102 42-43 -0.1674164268288436 1102 44-45 0.1522849770078878 1102 46-47 0.4188443531324566 1102 48-49 0.1299623485925565 1102 50-51 0.045585532073985746 1102 52-53 -0.012223653482820396 1102 54-55 -0.04617006696527426 1102 56-57 -0.06095248107314433 1102 58-59 0.017991090517448072 1102 60-61 0.11736595235507963 1102 62-63 0.19938416436200157 1102 64-65 0.3653241699102594 1102 66-67 0.20289341456425092 1102 68-69 0.2635253410688705 1102 70-71 0.44273916256291557 1102 72-73 0.4196224257387442 1102 74-75 0.2175079562758242 1102 76-77 0.14872986193045534 1102 78-79 0.17923925931391693 1102 80-81 0.13421692789730955 1102 82-83 0.12128976366735955 1102 84-85 0.27965659701904144 1102 86-87 0.1790105308363934 1102 88-89 0.1564674214913211 1102 90-91 0.037939687392935184 1102 92-93 0.21280031538092103 1102 94-95 0.10876878908270271 1102 96-97 0.14361009407898706 1102 98-99 -0.0013754879090761563 1102 100 0.1084246227878296 1103 1 0.17597383254423704 1103 2 0.3001772224094523 1103 3 0.24884590562890452 1103 4 0.08655715279009968 1103 5 0.016318154356326886 1103 6 0.1164336828858552 1103 7 0.12531449718343168 1103 8 0.16901578906618653 1103 9 0.2333417925831327 1103 10-11 0.23469619634463612 1103 12-13 0.18237611549120913 1103 14-15 0.3099247413846058 1103 16-17 0.255557916172954 1103 18-19 0.34525805365269235 1103 20-21 0.21895396898677433 1103 22-23 0.2855274802468415 1103 24-25 0.2268530015530743 1103 26-27 0.14278829949341798 1103 28-29 0.09402827797897118 1103 30-31 0.39871555343589193 1103 32-33 0.43648680090001335 1103 34-35 0.3048736325854051 1103 36-37 0.37383698437905366 1103 38-39 0.19830591424148025 1103 40-41 -0.048466702762596015 1103 42-43 0.056380588050338076 1103 44-45 0.030172025235614797 1103 46-47 0.04331530275371165 1103 48-49 0.16796112607028846 1103 50-51 0.10331489040454045 1103 52-53 0.1847823275913001 1103 54-55 -0.09964574418845018 1103 56-57 -0.045475581327700354 1103 58-59 0.08247333686342273 1103 60-61 -0.015799993436111492 1103 62-63 0.028064691439325884 1103 64-65 0.025037418801865385 1103 66-67 0.19454273637389008 1103 68-69 0.20721000769943032 1103 70-71 0.20758158872798305 1103 72-73 0.36024304922510453 1103 74-75 0.27431655416658884 1103 76-77 0.11669088049268339 1103 78-79 0.1923678330637344 1103 80-81 0.20571320229939616 1103 82-83 0.14559319678842897 1103 84-85 0.26499987043846573 1103 86-87 0.22731950399506218 1103 88-89 0.14910907545561614 1103 90-91 0.15483564146323658 1103 92-93 0.2848440166831345 1103 94-95 0.3380245979978298 1103 96-97 0.44819029695578294 1103 98-99 0.32374579713205165 1103 100 0.2881237894609434 1104 1 0.22030016258485574 1104 2 0.15847336641988363 1104 3 0.26060441738741247 1104 4 0.2968565059466002 1104 5 0.25397046343721286 1104 6 0.21275820778180332 1104 7 0.28883051784230673 1104 8 0.2534564449354164 1104 9 0.2637534658519485 1104 10-11 0.36309964617665713 1104 12-13 0.2901081517946693 1104 14-15 0.45708163854150996 1104 16-17 0.38329697248343564 1104 18-19 0.4611132409364487 1104 20-21 0.4835831621874007 1104 22-23 0.40101913145277024 1104 24-25 0.38212051396344293 1104 26-27 0.5714349424257748 1104 28-29 0.5517076642297951 1104 30-31 0.6389878294224545 1104 32-33 0.6171642315774406 1104 34-35 0.7718921353181969 1104 36-37 0.7656661976368326 1104 38-39 0.7886007545363114 1104 40-41 0.6039050567520121 1104 42-43 0.5880834411817659 1104 44-45 0.6428932808140146 1104 46-47 0.7704250655777543 1104 48-49 0.7567892863270202 1104 50-51 0.8897562482744661 1104 52-53 0.8927625712858216 1104 54-55 0.9940588138018214 1104 56-57 0.9686521828000565 1104 58-59 0.8787050973808945 1104 60-61 0.9946046598256828 1104 62-63 0.8963142025459803 1104 64-65 0.9420509072439245 1104 66-67 0.9454453087050325 1104 68-69 1.0142475647369906 1104 70-71 1.0945344042522294 1104 72-73 1.0323711142103136 1104 74-75 1.0370152454367059 1104 76-77 1.0114534538266753 1104 78-79 0.9788217266604917 1104 80-81 0.853521957251008 1104 82-83 0.8660966315775731 1104 84-85 0.964581177162632 1104 86-87 1.0129585753484207 1104 88-89 1.0364630342381496 1104 90-91 0.9992798073359737 1104 92-93 0.913585931139334 1104 94-95 0.8435779249797264 1104 96-97 0.9772105045474184 1104 98-99 0.9376454110316637 1104 100 1.0017325744983019 1105 1 -0.06676815756617316 1105 2 0.03178326454180791 1105 3 0.11079808337055397 1105 4 0.07170922215269826 1105 5 0.09851689444980849 1105 6 0.15593107501482706 1105 7 0.06658659635026964 1105 8 0.03306781101294831 1105 9 0.17305594282360204 1105 10-11 0.07311657160711604 1105 12-13 0.22757356858339506 1105 14-15 0.2720394877098826 1105 16-17 0.19760221611198858 1105 18-19 0.15759633178044652 1105 20-21 0.2486972460984802 1105 22-23 0.22186833237716996 1105 24-25 0.1899973489079514 1105 26-27 0.32917995430612734 1105 28-29 0.4304808960105362 1105 30-31 0.3573079804493702 1105 32-33 0.34041554167085764 1105 34-35 0.37401269646130686 1105 36-37 0.26073666601557477 1105 38-39 0.08780330637045353 1105 40-41 -0.07054893537114992 1105 42-43 -0.2467048281929749 1105 44-45 -0.3119874274501484 1105 46-47 -0.17277221333380766 1105 48-49 -0.13407098122497985 1105 50-51 -0.0893017864226664 1105 52-53 -0.20613076595337532 1105 54-55 -0.26022624950579143 1105 56-57 -0.41970507397824974 1105 58-59 -0.30998415822565306 1105 60-61 -0.14386000833823687 1105 62-63 -0.16781690444227593 1105 64-65 -0.24968655329053036 1105 66-67 -0.25547419785357306 1105 68-69 -0.240229537108533 1105 70-71 -0.28243399076128384 1105 72-73 -0.2931065409665905 1105 74-75 -0.20388570824619734 1105 76-77 -0.44317500042583546 1105 78-79 -0.18388699319109136 1105 80-81 -0.25604253840371527 1105 82-83 -0.17401663861088323 1105 84-85 -0.06554608905485892 1105 86-87 -0.11796939392541361 1105 88-89 -0.1590566090258534 1105 90-91 -0.2084407928658294 1105 92-93 -0.15639421402877574 1105 94-95 -0.2602921397925897 1105 96-97 -0.3762559538062611 1105 98-99 -0.21736712029665384 1105 100 -0.20995248229953845 1106 1 -0.06903307912934054 1106 2 0.08286467176356638 1106 3 0.06740255751888924 1106 4 0.02921409544704545 1106 5 0.02258632729117238 1106 6 -0.026757126971624245 1106 7 -0.0022892970870316276 1106 8 -0.06402677730971362 1106 9 -0.07251167993700136 1106 10-11 0.01715722880566517 1106 12-13 0.04361831006673356 1106 14-15 0.05785123931110547 1106 16-17 -1.9783958280328307E-4 1106 18-19 -0.043559335164694346 1106 20-21 -0.08678033674753038 1106 22-23 -0.03590109118172791 1106 24-25 -0.1195171781504385 1106 26-27 -0.03252987582475697 1106 28-29 0.10568278963348376 1106 30-31 0.0507127887664609 1106 32-33 -0.01695473587485452 1106 34-35 -0.08133400362223142 1106 36-37 -0.11065941678401714 1106 38-39 -0.27160447233557505 1106 40-41 -0.1014363390655717 1106 42-43 -0.11756716923075317 1106 44-45 -0.08017641844615753 1106 46-47 -0.09578808634967828 1106 48-49 -0.292856834747667 1106 50-51 -0.22017526641894847 1106 52-53 -0.32273709659479266 1106 54-55 -0.24306297093900753 1106 56-57 -0.2825102350290223 1106 58-59 -0.301017296627208 1106 60-61 -0.3466308242669456 1106 62-63 -0.3535311901565592 1106 64-65 -0.3915458311147191 1106 66-67 -0.3435787184142285 1106 68-69 -0.41127752745477153 1106 70-71 -0.36944782163476475 1106 72-73 -0.31086853855315155 1106 74-75 -0.35345407360404124 1106 76-77 -0.35600800887287676 1106 78-79 -0.42621503551913165 1106 80-81 -0.43703669045049764 1106 82-83 -0.5554888376269425 1106 84-85 -0.39900937690411453 1106 86-87 -0.4774328674239747 1106 88-89 -0.5163897567098807 1106 90-91 -0.6049598683322728 1106 92-93 -0.5386461401403544 1106 94-95 -0.6217327784262139 1106 96-97 -0.6350000362345511 1106 98-99 -0.6073281339418806 1106 100 -0.5370108690070481 1107 1 -0.0732617405168341 1107 2 -0.010186592987921017 1107 3 -0.14382336959071296 1107 4 -0.08071630475181024 1107 5 -0.049487344335076955 1107 6 -0.005596064982825055 1107 7 -0.07135052834065192 1107 8 -0.08976864253703809 1107 9 -0.06622935094773652 1107 10-11 0.057430123944335776 1107 12-13 -0.07367451807620284 1107 14-15 -0.021269708098429874 1107 16-17 -0.041509405223230544 1107 18-19 -0.03175666480498052 1107 20-21 -0.06723646401574257 1107 22-23 -0.08264126376083425 1107 24-25 -0.12383757396971617 1107 26-27 -0.08310957825009524 1107 28-29 0.0871775874034455 1107 30-31 0.12454864923542885 1107 32-33 -0.16609864648409456 1107 34-35 -0.2654050593375814 1107 36-37 -0.14327840521956148 1107 38-39 -0.12506611550639235 1107 40-41 -0.0012860129644565177 1107 42-43 0.12871610669458278 1107 44-45 -0.04876477349984043 1107 46-47 0.019397809037556613 1107 48-49 6.164083900017658E-4 1107 50-51 0.14746378522457348 1107 52-53 0.20411002057671368 1107 54-55 0.2937328933177099 1107 56-57 0.11607439146388288 1107 58-59 0.1524378363581249 1107 60-61 0.23966760263550668 1107 62-63 -0.03649699392772732 1107 64-65 0.024804203803547864 1107 66-67 0.20584394650060034 1107 68-69 0.19746032513095457 1107 70-71 0.29824715842039495 1107 72-73 0.4573673787319805 1107 74-75 0.4230364629536112 1107 76-77 0.360507443940115 1107 78-79 0.5280257584867627 1107 80-81 0.6842347487739637 1107 82-83 0.3750119731870676 1107 84-85 0.3751226970042474 1107 86-87 0.38885975949504825 1107 88-89 0.41549890728169103 1107 90-91 0.41934679691398813 1107 92-93 0.4161213870544671 1107 94-95 0.3148520966374093 1107 96-97 0.30489911507399725 1107 98-99 0.2995821320287888 1107 100 0.6029640371870961 1108 1 -0.08747846993244934 1108 2 -0.13642383858185525 1108 3 -0.017528831477541473 1108 4 0.043125567895103245 1108 5 -0.1554839613482244 1108 6 -0.013559725156333968 1108 7 -0.15164190172419012 1108 8 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-0.41726509905824116 2215 16-17 -0.4157776190180016 2215 18-19 -0.4081191617727171 2215 20-21 -0.48680264821562247 2215 22-23 -0.502682502541461 2215 24-25 -0.5328413744063596 2215 26-27 -0.70726054453133 2215 28-29 -0.673841657360839 2215 30-31 -0.8435903497133879 2215 32-33 -0.7635899539960249 2215 34-35 -0.9138545620464029 2215 36-37 -0.9644799900825021 2215 38-39 -0.9991867591548171 2215 40-41 -0.7502995166436079 2215 42-43 -0.5599472716510121 2215 44-45 -0.6929428279997154 2215 46-47 -0.6910453057032839 2215 48-49 -0.32776153290538446 2215 50-51 -0.2981899707388749 2215 52-53 -0.1892210217132586 2215 54-55 -0.35830276609848255 2215 56-57 -0.30977059923717576 2215 58-59 -0.255725016395175 2215 60-61 -0.29949306688821764 2215 62-63 -0.1609895952053222 2215 64-65 -0.25876497222944295 2215 66-67 -0.14588848658745235 2215 68-69 -0.26778185162977763 2215 70-71 -0.1795910400109122 2215 72-73 -0.24271550313103774 2215 74-75 -0.2382759005945374 2215 76-77 -0.38239503847275813 2215 78-79 -0.3820797642754279 2215 80-81 -0.39875021120016285 2215 82-83 -0.4147268478689661 2215 84-85 -0.543394324100305 2215 86-87 -0.3436299308097901 2215 88-89 -0.3514049497571996 2215 90-91 -0.33541183492882 2215 92-93 -0.30375206649125985 2215 94-95 -0.3002202733795727 2215 96-97 -0.4073593146179242 2215 98-99 -0.35830983853804454 2215 100 -0.4316603664677885 2216 1 -0.27346145847521086 2216 2 -0.27606804690512377 2216 3 -0.22100088600006984 2216 4 -0.2267448337792075 2216 5 -0.3030811600924892 2216 6 -0.19168631830345362 2216 7 -0.1600939664428509 2216 8 -0.12540593109810771 2216 9 -0.1891408031667865 2216 10-11 -0.36376895905121387 2216 12-13 -0.26175825933624 2216 14-15 -0.29093602036724064 2216 16-17 -0.26637207605407554 2216 18-19 -0.29537975530195126 2216 20-21 -0.36694168621580303 2216 22-23 -0.29898454238399097 2216 24-25 -0.30735659701295504 2216 26-27 -0.43307469221115724 2216 28-29 -0.5369124222686565 2216 30-31 -0.45417658906021074 2216 32-33 -0.3872290648950596 2216 34-35 -0.47470191184162047 2216 36-37 -0.4414432888220148 2216 38-39 -0.2883069362327575 2216 40-41 -0.2047768318450096 2216 42-43 -0.20982798261367464 2216 44-45 -0.21777748013963105 2216 46-47 -0.12871280739321378 2216 48-49 -0.12739505047400712 2216 50-51 -0.03353199891759573 2216 52-53 0.02498497719988535 2216 54-55 0.012965806541913594 2216 56-57 -0.029397622180439953 2216 58-59 -0.049275455776459864 2216 60-61 -0.01984576480862188 2216 62-63 -0.08596871962379282 2216 64-65 -0.1357527914932959 2216 66-67 -0.009110350942570733 2216 68-69 0.13789564383061048 2216 70-71 -0.15420169384738358 2216 72-73 -0.1693123194391788 2216 74-75 -0.29080018916797457 2216 76-77 -0.08110274056826938 2216 78-79 -0.13416202069383942 2216 80-81 -0.25276826360781257 2216 82-83 -0.11647509498283526 2216 84-85 -0.18234882195973157 2216 86-87 -0.255830548461919 2216 88-89 -0.3964690344789261 2216 90-91 -0.4060495011050733 2216 92-93 -0.5658956904922157 2216 94-95 -0.4568123181262109 2216 96-97 -0.5363306454859504 2216 98-99 -0.6164957173668917 2216 100 -0.7292507279135734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 29.0 6 84.0 7 147.0 8 223.0 9 378.0 10 667.0 11 1143.0 12 1632.0 13 2416.0 14 2654.0 15 3557.0 16 4614.0 17 6664.0 18 7151.0 19 3095.0 20 1532.0 21 1328.0 22 1377.0 23 1521.0 24 1829.0 25 2254.0 26 2629.0 27 3073.0 28 2764.0 29 3563.0 30 4267.0 31 5360.0 32 6987.0 33 8712.0 34 12290.0 35 18774.0 36 31538.0 37 68715.0 38 132208.0 39 65602.0 40 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78086844315678 20.85359173189526 14.333816207530404 22.031723617417555 2 16.016428106304677 22.25062129751798 39.72914110953128 22.003809486646066 3 17.477441083996066 27.093574480925913 33.08835697111875 22.340627463959272 4 11.83822759981797 18.965596960987824 39.04502790060425 30.151147538589957 5 11.280914406971453 38.4556606551433 36.178403778718184 14.085021159167066 6 27.501143741543615 40.14605822861203 17.522363797416602 14.83043423242775 7 23.101089472006386 35.94576244789297 23.09841263660986 17.85473544349079 8 24.0501400485242 34.4891696619124 21.953427477739464 19.507262811823942 9 24.768271226696324 15.214889045172814 22.847763504026204 37.16907622410466 10-11 22.375383731838518 27.128122326957172 29.622820348403568 20.873673592800746 12-13 24.39278422516566 25.492720224464815 30.0529526711167 20.061542879252823 14-15 21.64966265749241 26.797710086206372 29.15527489307855 22.39735236322267 16-17 18.623496559653958 30.024760757545337 29.985581573616106 21.3661611091846 18-19 18.442246457134583 26.892194927865106 34.71344980854473 19.952108806455577 20-21 20.30915015343134 25.99146332857181 34.52387615499364 19.175510363003216 22-23 19.477164741167954 26.500459321178056 34.62928826510437 19.393087672549626 24-25 19.71839554572422 26.542290554862007 34.4982490977804 19.241064801633364 26-27 19.17928226742559 26.52731710522153 34.655041089423335 19.63835953792954 28-29 19.074688756290577 26.210175892848454 34.998004536030294 19.71713081483068 30-31 20.149270075657103 26.90973954391592 34.16286839947145 18.778121980955532 32-33 19.49295870616378 26.42876089289495 34.87368987158491 19.204590529356366 34-35 19.6697515166706 26.202324953216216 34.71332309646585 19.414600433647333 36-37 20.105832337631682 26.4125782061796 33.8501653554229 19.63142410076582 38-39 19.849829534254976 26.108757388674064 34.32177508255604 19.719637994514922 40-41 19.74652802281637 25.7655140862379 34.31277118167682 20.175186709268907 42-43 20.09220481197665 25.678273587178445 33.904432109370624 20.32508949147428 44-45 20.139901151769266 26.30684320801688 33.26479012393747 20.28846551627638 46-47 20.706416861142817 26.641325958246238 32.02310352295873 20.62915365765222 48-49 20.741215721297632 26.469765144196256 31.339293753482927 21.449725381023182 50-51 20.556514078937443 26.49239657073051 30.99617212538297 21.95491722494908 52-53 20.412086641861325 26.689630669719882 31.139869516441856 21.75841317197694 54-55 20.47730408606756 27.35506761442863 30.633825952162518 21.533802347341293 56-57 20.54008804354968 26.95220388725169 30.13143261796935 22.376275451229276 58-59 19.797752918358956 26.75387471923647 30.911365113047623 22.537007249356954 60-61 20.404421158680368 27.180221593301095 30.037135007409965 22.378222240608565 62-63 19.780961859962574 27.532833819625097 29.979461372048483 22.706742948363846 64-65 19.719637994514922 27.268070464041582 30.167691570158638 22.844599971284858 66-67 19.58411661282571 27.294263656439927 29.90101780706077 23.220601923673595 68-69 20.31696203147719 27.46509463233013 29.500708753870768 22.717234582321915 70-71 20.411234921507887 27.104053458836358 29.356975468020334 23.127736151635425 72-73 20.107560244079014 26.94615295699415 29.62238323812305 23.32390356080378 74-75 20.460391353334973 27.135080414568797 29.247833588443857 23.15669464365237 76-77 20.571236673618326 27.063414230543664 29.15219756018621 23.213151535651797 78-79 20.835513331857015 26.782346513908596 29.333005623787816 23.04913453044657 80-81 20.776744627469686 26.80789812451178 29.18602302565138 23.22933422236715 82-83 20.455037682541924 26.854012697933726 29.429980069743728 23.260969549780622 84-85 20.48448287190369 26.758741692684694 29.059603390333706 23.697172045077906 86-87 20.72140801713177 26.886045761773524 28.738418110030235 23.65412811106447 88-89 20.599647867187283 26.605860687303874 28.79872093292077 23.99577051258808 90-91 20.547835196805266 26.447430379885457 28.767309969057724 24.237424454251553 92-93 20.574185384372036 26.614143528094807 28.53332684398803 24.27834424354513 94-95 20.296326760056115 26.379944102605908 28.696875269965922 24.626853867372056 96-97 20.320222518026053 26.467575006144557 28.555384941097454 24.65681753473194 98-99 20.481684362170963 26.27569457794823 28.360584328832196 24.882036731048615 100 20.5619894240667 26.45224403978264 28.074649638748895 24.911116897401765 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 322.0 1 204.5 2 179.0 3 259.5 4 152.0 5 88.5 6 192.5 7 375.5 8 583.0 9 650.5 10 426.0 11 206.5 12 184.0 13 214.5 14 249.5 15 273.5 16 307.5 17 331.5 18 376.0 19 441.0 20 523.0 21 667.0 22 870.0 23 1209.0 24 1665.5 25 2099.5 26 2775.0 27 3818.5 28 5350.5 29 6807.0 30 7636.5 31 8840.5 32 10352.5 33 11872.0 34 13503.5 35 14703.5 36 15216.0 37 15610.0 38 15900.0 39 15860.0 40 15875.0 41 15518.0 42 14681.0 43 14008.5 44 13909.0 45 13790.5 46 13487.5 47 13274.5 48 13144.0 49 12846.0 50 12466.5 51 11907.0 52 10823.5 53 9993.5 54 9339.5 55 8702.0 56 7965.5 57 7064.5 58 6152.5 59 5080.5 60 4058.5 61 3218.0 62 2591.0 63 2079.5 64 1641.0 65 1158.0 66 787.0 67 613.5 68 503.0 69 422.5 70 302.0 71 235.0 72 287.5 73 294.5 74 216.0 75 244.0 76 283.5 77 171.0 78 87.0 79 148.5 80 155.5 81 116.5 82 100.5 83 45.5 84 6.5 85 2.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0481830371374409 2 0.21998719985983117 3 0.002190138051701859 4 0.002433486724113177 5 0.0 6 2.4334867241131765E-4 7 0.0 8 9.733946896452706E-4 9 0.0 10-11 3.6502300861697645E-4 12-13 0.0 14-15 1.2167433620565883E-4 16-17 1.2167433620565883E-4 18-19 3.6502300861697645E-4 20-21 0.0 22-23 1.2167433620565883E-4 24-25 4.866973448226353E-4 26-27 0.0 28-29 2.4334867241131765E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.2167433620565883E-4 68-69 1.2167433620565883E-4 70-71 0.0 72-73 1.2167433620565883E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.2167433620565883E-4 88-89 0.0020684637154962 90-91 0.0015817663706735648 92-93 7.300460172339529E-4 94-95 3.6502300861697645E-4 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 410933.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.35607539958859 #Duplication Level Percentage of deduplicated Percentage of total 1 88.09881317486638 67.26879621394366 2 9.070291668588478 13.851437490860045 3 1.521582520692625 3.485462090301064 4 0.422405433622031 1.2901288455535886 5 0.2072001792406928 0.7910496254455307 6 0.11982052704605976 0.548941511852843 7 0.07549893356607836 0.40353615847720065 8 0.062066461366680536 0.37913211231199295 9 0.04377328751616684 0.30081207978650754 >10 0.32000117898538843 4.77492124206721 >50 0.03563708192940873 1.8970008051844327 >100 0.021000423198379928 2.7969099903169288 >500 6.363764605569676E-4 0.32799066078247513 >1k 0.0012727529211139352 1.883881173116528 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2435 0.5925540173215585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2402 0.584523511131985 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1695 0.4124759997371834 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1222 0.29737207768663015 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 697 0.16961402467068842 RNA PCR Primer, Index 23 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.15890668308459044 No Hit ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 464 0.1129137839988514 RNA PCR Primer, Index 23 (96% over 25bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 2.4334867241131765E-4 0.0 0.0 10-11 0.0 0.0 2.4334867241131765E-4 0.0 0.0 12-13 0.0 0.0 2.4334867241131765E-4 2.4334867241131765E-4 0.0 14-15 0.0 0.0 2.4334867241131765E-4 2.4334867241131765E-4 0.0 16-17 0.0 0.0 2.4334867241131765E-4 6.083716810282942E-4 0.0 18-19 0.0 0.0 2.4334867241131765E-4 9.733946896452706E-4 0.0 20-21 0.0 0.0 2.4334867241131765E-4 0.0013384176982622472 0.0 22-23 0.0 0.0 2.4334867241131765E-4 0.002433486724113177 0.0 24-25 0.0 0.0 2.4334867241131765E-4 0.005718693801665965 0.0 26-27 0.0 0.0 2.4334867241131765E-4 0.01253245662918286 0.0 28-29 0.0 0.0 2.4334867241131765E-4 0.02920184068935812 0.0 30-31 0.0 0.0 2.4334867241131765E-4 0.06923269730101987 0.0 32-33 0.0 0.0 2.4334867241131765E-4 0.14102055566235858 0.0 34-35 0.0 0.0 2.4334867241131765E-4 0.26610177328177587 0.0 36-37 0.0 0.0 2.4334867241131765E-4 0.4441113271506547 0.0 38-39 0.0 0.0 2.4334867241131765E-4 0.6852698615102706 0.0 40-41 0.0 0.0 2.4334867241131765E-4 0.9538051215161596 0.0 42-43 0.0 0.0 2.4334867241131765E-4 1.1835262682724434 0.0 44-45 0.0 0.0 2.4334867241131765E-4 1.4391640486405328 0.0 46-47 0.0 0.0 2.4334867241131765E-4 1.747851839594289 0.0 48-49 0.0 0.0 2.4334867241131765E-4 2.053862795151521 0.0 50-51 0.0 0.0 2.4334867241131765E-4 2.364740724156979 0.0 52-53 0.0 0.0 2.4334867241131765E-4 2.6698999593607717 0.0 54-55 0.0 0.0 2.4334867241131765E-4 3.0058428016245955 0.0 56-57 0.0 0.0 2.4334867241131765E-4 3.4061513677412134 0.0 58-59 0.0 0.0 2.4334867241131765E-4 3.82471108428868 0.0 60-61 0.0 0.0 2.4334867241131765E-4 4.259940184896321 0.0 62-63 0.0 0.0 2.4334867241131765E-4 4.661587168711201 0.0 64-65 0.0 0.0 2.4334867241131765E-4 5.091340924189588 0.0 66-67 0.0 0.0 2.4334867241131765E-4 5.594464304399987 0.0 68-69 0.0 0.0 2.4334867241131765E-4 6.182516371281936 0.0 70-71 0.0 0.0 2.4334867241131765E-4 6.797215117792925 0.0 72-73 0.0 0.0 2.4334867241131765E-4 7.4160507917349054 0.0 74-75 0.0 0.0 2.4334867241131765E-4 8.05216422141809 0.0 76-77 2.4334867241131765E-4 0.0 2.4334867241131765E-4 8.742301056376586 0.0 78-79 2.4334867241131765E-4 0.0 2.4334867241131765E-4 9.506537562084329 0.0 80-81 2.4334867241131765E-4 0.0 2.4334867241131765E-4 10.301070977507283 0.0 82-83 2.4334867241131765E-4 0.0 2.4334867241131765E-4 11.131254973438491 0.0 84-85 2.4334867241131765E-4 0.0 2.4334867241131765E-4 11.96180399237832 0.0 86-87 2.4334867241131765E-4 0.0 2.4334867241131765E-4 12.839440979429735 0.0 88 2.4334867241131765E-4 0.0 2.4334867241131765E-4 13.536269902879544 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGCG 45 2.2005406E-7 62.663017 9 GGTATCA 1510 0.0 51.511326 1 GTATCAA 2710 0.0 38.617077 1 GTACACG 75 3.2730267E-4 31.331505 4 ATCAACG 3320 0.0 31.28813 3 TATCAAC 3375 0.0 31.124968 2 TCAACGC 3360 0.0 30.911888 4 CAACGCA 3450 0.0 30.241716 5 CGTGCGC 55 2.9684343E-6 29.90735 10-11 TACTAGG 65 0.0060821967 28.988413 2 AACGCAG 3630 0.0 28.742128 6 ATAGGAC 85 6.8060576E-4 27.648813 3 CGCAGAG 4035 0.0 25.740759 8 CGTGCTC 55 1.0183934E-4 25.634872 18-19 ACGCAGA 4145 0.0 25.057646 7 GTACTAT 95 0.0012806538 24.810877 1 GCAGAGT 4265 0.0 24.242432 9 ATCTAAG 160 1.9813142E-6 23.570335 1 GTCCTAA 140 1.8833342E-5 23.570335 1 CGCTCGT 60 1.8381506E-4 23.49863 14-15 >>END_MODULE