FastQCFastQC Report
Wed 25 May 2016
SRR1033096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797871
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.035.1166841
GGTATCA31400.035.0360151
TCAACGC49050.033.1625864
ATCAACG51850.031.4624123
CAACGCA52350.031.2521295
AACGCAG55300.029.5748356
GTACATG118000.028.7264351
TACATGG116900.027.9097712
TATCAAC61050.027.4912092
ACATGGG119050.026.0235983
ACGCAGA66850.024.1138557
CGCAGAG70550.022.8000138
TATACCG2555.5842975E-1022.1237185
CATGGGG85350.022.0877864
TACACCG5350.018.4536635
GAGTACA81600.018.2064571
GCAGAGT84900.018.1189089
ACCGTCC4450.017.9678028
GACGCGT1551.2432974E-716.6996110-11
AGTACAT83100.016.6890752
Produced by FastQC (version 0.11.5)