##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033096_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3797871
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66013958873274	33.0	31.0	34.0	30.0	34.0
2	31.710879332131082	33.0	31.0	34.0	30.0	34.0
3	31.769207010980626	34.0	31.0	34.0	30.0	34.0
4	35.1454264770973	37.0	35.0	37.0	33.0	37.0
5	35.192326964238646	37.0	35.0	37.0	33.0	37.0
6	35.25578119951941	37.0	35.0	37.0	33.0	37.0
7	35.14046001035844	37.0	35.0	37.0	33.0	37.0
8	35.14446909860814	37.0	35.0	37.0	33.0	37.0
9	36.764317956033786	39.0	37.0	39.0	33.0	39.0
10-11	36.76347669523267	39.0	37.5	39.0	33.0	39.0
12-13	36.725836132928166	39.0	37.5	39.0	33.0	39.0
14-15	38.119526176639496	41.0	39.0	41.0	33.0	41.0
16-17	38.135339904909884	41.0	38.5	41.0	33.0	41.0
18-19	38.13905922555031	41.0	38.5	41.0	33.5	41.0
20-21	38.14506258901369	40.5	39.0	41.0	33.5	41.0
22-23	38.120046336486936	40.0	39.0	41.0	33.0	41.0
24-25	38.13196446114152	40.0	38.0	41.0	33.0	41.0
26-27	38.041605941855316	40.0	38.0	41.0	33.0	41.0
28-29	38.05174543843116	40.0	38.0	41.0	33.0	41.0
30-31	38.20703072853185	40.0	39.0	41.0	33.0	41.0
32-33	38.19200559997957	41.0	38.5	41.0	33.0	41.0
34-35	38.1501680810117	40.0	38.0	41.0	33.0	41.0
36-37	38.08132806511859	40.0	38.0	41.0	33.0	41.0
38-39	37.9936061546061	40.0	38.0	41.0	33.0	41.0
40-41	37.92000689333577	40.0	38.0	41.0	33.0	41.0
42-43	37.806569522766836	40.0	38.0	41.0	32.5	41.0
44-45	37.68276239503659	40.0	38.0	41.0	32.0	41.0
46-47	37.55042496177464	40.0	38.0	41.0	32.0	41.0
48-49	37.43357633790089	40.0	37.5	41.0	31.5	41.0
50-51	37.304249802060156	40.0	37.0	41.0	31.0	41.0
52-53	37.14813707469264	40.0	37.0	41.0	31.0	41.0
54-55	36.984531465128754	40.0	36.5	41.0	31.0	41.0
56-57	36.8087822098223	40.0	36.0	41.0	30.5	41.0
58-59	36.610345770038	39.5	35.5	41.0	30.0	41.0
60-61	36.380186162194555	39.0	35.0	41.0	30.0	41.0
62-63	36.12344495113183	39.0	35.0	41.0	30.0	41.0
64-65	35.844143205495925	38.5	35.0	40.5	30.0	41.0
66-67	35.537274436124875	38.0	35.0	40.0	29.0	41.0
68-69	35.21637188308924	37.0	35.0	40.0	29.0	41.0
70-71	34.878926114130785	37.0	34.0	39.5	29.0	41.0
72-73	34.524516235543544	36.0	34.0	39.0	29.0	41.0
74-75	34.166993955297585	36.0	34.0	39.0	28.0	40.5
76-77	33.807090209225116	35.0	34.0	38.0	28.0	40.0
78-79	33.42464501822205	35.0	34.0	37.0	27.5	39.0
80-81	33.08335367367665	35.0	33.5	37.0	27.0	39.0
82-83	32.87358259403756	35.0	33.5	36.0	27.0	38.5
84-85	32.65880436697297	35.0	33.5	36.0	27.0	37.0
86-87	32.49189888229485	35.0	34.0	36.0	27.0	37.0
88-89	32.28535131919962	35.0	33.0	35.0	27.0	37.0
90-91	32.086110613025035	35.0	33.0	35.0	27.0	36.0
92-93	31.881582865768742	35.0	33.0	35.0	26.0	36.0
94-95	31.694284771652327	35.0	33.0	35.0	26.0	36.0
96-97	31.528985581658777	35.0	33.0	35.0	25.0	35.5
98-99	31.364132431038335	35.0	33.0	35.0	25.0	35.0
100	31.233151415622068	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0662162052511448
1101	2	0.12013853782203654
1101	3	0.10793309029662623
1101	4	0.001843208208128999
1101	5	-0.03249796510694125
1101	6	0.12125948874255954
1101	7	0.14317989564633393
1101	8	0.18767100159757888
1101	9	0.23805507382267876
1101	10-11	0.24296097416380746
1101	12-13	0.2730679623808143
1101	14-15	0.3027835584696277
1101	16-17	0.331966082535871
1101	18-19	0.34080118452042285
1101	20-21	0.36511251265512357
1101	22-23	0.3731178847574128
1101	24-25	0.30364000659278645
1101	26-27	0.2034672209095234
1101	28-29	0.21672188511278279
1101	30-31	0.25973146997454677
1101	32-33	0.2575441996948271
1101	34-35	0.25271049432816284
1101	36-37	0.26971134627468274
1101	38-39	0.2513354409042563
1101	40-41	0.2650591838032952
1101	42-43	0.3302670416111937
1101	44-45	0.29177634732456426
1101	46-47	0.28471295664314766
1101	48-49	0.34954446617526713
1101	50-51	0.25039052018308894
1101	52-53	0.18845214086318407
1101	54-55	0.18976712375486215
1101	56-57	0.25804933472149827
1101	58-59	0.2592504046209996
1101	60-61	0.33017109531832745
1101	62-63	0.2828345480470844
1101	64-65	0.3048978774792488
1101	66-67	0.19932355762114895
1101	68-69	0.16902212645062775
1101	70-71	0.07139019369489574
1101	72-73	0.07652298138944502
1101	74-75	0.1596207660224067
1101	76-77	0.1359366260546082
1101	78-79	0.14997166786896798
1101	80-81	0.11227483362370805
1101	82-83	0.14815931781629388
1101	84-85	0.09633451992770148
1101	86-87	0.0781560281199063
1101	88-89	0.12151988630668598
1101	90-91	0.1681115743782513
1101	92-93	0.1429514621964607
1101	94-95	0.18646757219745425
1101	96-97	0.24119497066395112
1101	98-99	0.21666658303817954
1101	100	0.21845519915243372
1102	1	-0.024371054423252048
1102	2	0.022684240160270264
1102	3	0.021094732711379294
1102	4	-0.08384312021679108
1102	5	-0.05420528038271755
1102	6	0.0073148810220189375
1102	7	0.033591005974457744
1102	8	0.0575946464174919
1102	9	0.1045629320317758
1102	10-11	0.14928707084260395
1102	12-13	0.053575030232273946
1102	14-15	0.226015985332765
1102	16-17	0.20851006644500103
1102	18-19	0.14925103342670099
1102	20-21	0.20080252573102797
1102	22-23	0.16716011500296446
1102	24-25	0.13705509311989772
1102	26-27	0.019267833390628653
1102	28-29	0.07644785531594067
1102	30-31	0.18041170030208065
1102	32-33	0.16294153403406852
1102	34-35	0.13049361484986832
1102	36-37	0.16378689958259685
1102	38-39	0.19372402281565826
1102	40-41	0.11162916522804522
1102	42-43	0.19378987193900343
1102	44-45	0.20695107577729743
1102	46-47	0.17422610601106925
1102	48-49	0.09160910969219316
1102	50-51	0.18595219225928616
1102	52-53	0.13114476405373665
1102	54-55	0.11106217264326546
1102	56-57	0.07014192171034495
1102	58-59	0.10024251532264827
1102	60-61	0.05720236280603075
1102	62-63	0.052441727405302174
1102	64-65	0.058643728216807745
1102	66-67	0.06835787738161514
1102	68-69	0.051877399347389996
1102	70-71	0.07363541274509089
1102	72-73	0.08336451468326089
1102	74-75	0.084499656150264
1102	76-77	0.12051981603197959
1102	78-79	0.025998994622433713
1102	80-81	0.06728564807436044
1102	82-83	0.0555151017280906
1102	84-85	0.1413957288893215
1102	86-87	0.09712115704353863
1102	88-89	0.12556309989346914
1102	90-91	0.1390462989436827
1102	92-93	0.13758488317843032
1102	94-95	0.13180404761691733
1102	96-97	0.10816910994099516
1102	98-99	0.16844146790434777
1102	100	0.05069948782825762
1103	1	0.08148879934455167
1103	2	0.16257445222621314
1103	3	0.11996808158473016
1103	4	0.07183987647039203
1103	5	0.11386990338284164
1103	6	0.17045741027333605
1103	7	0.2458341324435267
1103	8	0.1912152099543718
1103	9	0.332573647490193
1103	10-11	0.29475080051135194
1103	12-13	0.30501036848770013
1103	14-15	0.407285958966753
1103	16-17	0.35733375554066527
1103	18-19	0.43380399789598556
1103	20-21	0.3834164018595416
1103	22-23	0.3560944373054937
1103	24-25	0.35655862538494176
1103	26-27	0.2514577726935414
1103	28-29	0.2954519451359303
1103	30-31	0.3011918383416301
1103	32-33	0.28350385177211734
1103	34-35	0.2746191313603177
1103	36-37	0.2821173719925767
1103	38-39	0.3216028269496931
1103	40-41	0.3565006349949087
1103	42-43	0.3375593985537222
1103	44-45	0.28485190577017505
1103	46-47	0.33181381119291586
1103	48-49	0.3017504328042264
1103	50-51	0.28008469852568396
1103	52-53	0.3488041386168774
1103	54-55	0.34222315485028076
1103	56-57	0.28386346283775055
1103	58-59	0.2869185775217318
1103	60-61	0.2336124241533284
1103	62-63	0.2640208270597171
1103	64-65	0.268726126060713
1103	66-67	0.2438913745678306
1103	68-69	0.23297059717699398
1103	70-71	0.2643452143335523
1103	72-73	0.24915949376275393
1103	74-75	0.23328514541692158
1103	76-77	0.2308132882501397
1103	78-79	0.23302052957284758
1103	80-81	0.2537296666313509
1103	82-83	0.27311026806803795
1103	84-85	0.2831414773296359
1103	86-87	0.3310942968744115
1103	88-89	0.2524751232490061
1103	90-91	0.30171701966879283
1103	92-93	0.3583920862032066
1103	94-95	0.3397996782066919
1103	96-97	0.32099132956237497
1103	98-99	0.3032116837415124
1103	100	0.27747317903709856
1104	1	0.11153353011146905
1104	2	0.17588651322555648
1104	3	0.18829294443013822
1104	4	0.14413914886137036
1104	5	0.08119875570624657
1104	6	0.12059963814239438
1104	7	0.10068650457680661
1104	8	0.19413757405630605
1104	9	0.19331391119371943
1104	10-11	0.23135173681137644
1104	12-13	0.2587952183291762
1104	14-15	0.28898062877685504
1104	16-17	0.2798183346710772
1104	18-19	0.3521280650708576
1104	20-21	0.29223949388032366
1104	22-23	0.2629846132247238
1104	24-25	0.14821761113173437
1104	26-27	0.09949417046627929
1104	28-29	0.13203832700229867
1104	30-31	0.19310689621645594
1104	32-33	0.17836651692480388
1104	34-35	0.23028197491220226
1104	36-37	0.22268113484670238
1104	38-39	0.2526504053721794
1104	40-41	0.335141809973571
1104	42-43	0.28433513029248303
1104	44-45	0.26609928903510394
1104	46-47	0.2146226309966366
1104	48-49	0.21436408518689376
1104	50-51	0.1915544512862084
1104	52-53	0.2002926551194335
1104	54-55	0.21044268640734032
1104	56-57	0.25522481569735334
1104	58-59	0.22128498225552562
1104	60-61	0.22171429786590835
1104	62-63	0.23474736861505363
1104	64-65	0.19869247271761026
1104	66-67	0.15293362805325472
1104	68-69	0.22752574622862198
1104	70-71	0.2636240410898836
1104	72-73	0.21845337066252313
1104	74-75	0.253374453449716
1104	76-77	0.20854018144961373
1104	78-79	0.19435058934389815
1104	80-81	0.21991254213961042
1104	82-83	0.2530215937551361
1104	84-85	0.18755873738109585
1104	86-87	0.16656448676083357
1104	88-89	0.25576078488567333
1104	90-91	0.26370375408058777
1104	92-93	0.22732451743861404
1104	94-95	0.29959177019916083
1104	96-97	0.30610570685019667
1104	98-99	0.3530110480218731
1104	100	0.37242800818691535
1105	1	0.027523044421954523
1105	2	0.04142922620635758
1105	3	0.03064985876479298
1105	4	0.07216785619829835
1105	5	0.02528405082048124
1105	6	0.112668644863966
1105	7	0.11648814371554295
1105	8	0.13811042326197054
1105	9	0.15591801549943796
1105	10-11	0.2269691182838045
1105	12-13	0.22587845695569087
1105	14-15	0.2518699222231362
1105	16-17	0.23305110611774182
1105	18-19	0.2180165483856129
1105	20-21	0.22296411441795527
1105	22-23	0.23829747634869847
1105	24-25	0.13287550739602239
1105	26-27	0.1753493888948796
1105	28-29	0.17207097405379557
1105	30-31	0.20085841687454575
1105	32-33	0.14957017840671938
1105	34-35	0.09409344837573741
1105	36-37	0.18100229238432064
1105	38-39	0.19163337136996006
1105	40-41	0.24792474568743472
1105	42-43	0.22724134639715032
1105	44-45	0.23630097297854036
1105	46-47	0.19426855228930862
1105	48-49	0.1702621452728863
1105	50-51	0.2018522522690347
1105	52-53	0.2729516812530406
1105	54-55	0.2623554591070416
1105	56-57	0.23460062635487589
1105	58-59	0.17337632921056922
1105	60-61	0.18586639526764515
1105	62-63	0.1472882133306257
1105	64-65	0.18136154250507275
1105	66-67	0.18826714154940305
1105	68-69	0.23307116298748554
1105	70-71	0.12591198437038997
1105	72-73	0.15861708549329023
1105	74-75	0.12283477253426867
1105	76-77	0.16207856397716824
1105	78-79	0.24280003139912054
1105	80-81	0.22570404077473682
1105	82-83	0.21662834352868998
1105	84-85	0.19973821227240052
1105	86-87	0.09411352062438993
1105	88-89	0.12603437476488466
1105	90-91	0.14034596696263435
1105	92-93	0.1470836581020727
1105	94-95	0.20038473928077494
1105	96-97	0.25527536378096727
1105	98-99	0.20002611844534002
1105	100	0.23822273091708013
1106	1	-0.07649862307646771
1106	2	-0.03998913147661298
1106	3	-0.010874986857494662
1106	4	-0.05990175809457554
1106	5	-0.04197327066260925
1106	6	-0.04678220586195181
1106	7	-0.019881521850265926
1106	8	0.03140661798197186
1106	9	-0.01888116602892609
1106	10-11	0.011826295822338295
1106	12-13	0.024040680100362977
1106	14-15	-0.02116892770355605
1106	16-17	0.05347802081274722
1106	18-19	0.045941466209150406
1106	20-21	0.00909134895903918
1106	22-23	0.006741144761150508
1106	24-25	-0.023512466707309443
1106	26-27	-0.06827808916322198
1106	28-29	-0.04571399628800776
1106	30-31	0.046115323607502035
1106	32-33	-0.03692666140525347
1106	34-35	-0.002534793958552939
1106	36-37	-0.0011725533858424342
1106	38-39	0.037706755812138226
1106	40-41	0.06143598335849987
1106	42-43	0.05058936834717542
1106	44-45	0.038247401521012137
1106	46-47	-0.05833005191526297
1106	48-49	-0.08794617549503414
1106	50-51	-0.04069864676596069
1106	52-53	-0.10596271393158219
1106	54-55	-0.08694780550948877
1106	56-57	-0.0817536150344722
1106	58-59	-0.057189922384772274
1106	60-61	-0.08616629920356189
1106	62-63	-0.09539807894851293
1106	64-65	-0.07083008693918913
1106	66-67	-0.06468629948857796
1106	68-69	-0.07178858153699252
1106	70-71	-0.09474211430509172
1106	72-73	-0.10095154383956384
1106	74-75	-0.03535050514621929
1106	76-77	-0.019962828416815626
1106	78-79	-0.10451941610485704
1106	80-81	-0.04642780957087922
1106	82-83	-0.06594448046654833
1106	84-85	-0.05314291303164964
1106	86-87	-0.0629173766068476
1106	88-89	-0.055466987006525414
1106	90-91	-0.08320360726973774
1106	92-93	0.033218496289570965
1106	94-95	0.009043985539033628
1106	96-97	-0.011236053025776016
1106	98-99	-0.05586835324576711
1106	100	0.007876924646097905
1107	1	0.06978677973630454
1107	2	0.17291759488422898
1107	3	0.14261308009595908
1107	4	0.18697620774785406
1107	5	0.21341593461825425
1107	6	0.2058678459945682
1107	7	0.218935627450783
1107	8	0.27945565904733627
1107	9	0.3016886051098737
1107	10-11	0.30687264975640005
1107	12-13	0.32231191976730855
1107	14-15	0.35804532424452873
1107	16-17	0.2911216335635487
1107	18-19	0.40348730166918045
1107	20-21	0.34246148065493287
1107	22-23	0.3207027382706329
1107	24-25	0.2856425951809456
1107	26-27	0.2449548147231937
1107	28-29	0.2579202476786904
1107	30-31	0.27001061534057413
1107	32-33	0.2130497964548681
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2216	42-43	-0.675360004801405
2216	44-45	-0.5989473665417577
2216	46-47	-0.5411950327387558
2216	48-49	-0.5961375447675579
2216	50-51	-0.5305370520154753
2216	52-53	-0.5605968431474864
2216	54-55	-0.5696720498040477
2216	56-57	-0.6611195899132838
2216	58-59	-0.5887587032050305
2216	60-61	-0.5824845751399579
2216	62-63	-0.6332100009344472
2216	64-65	-0.6409598174173823
2216	66-67	-0.6585187742861223
2216	68-69	-0.6894430799257307
2216	70-71	-0.6828341467885366
2216	72-73	-0.7072343711106086
2216	74-75	-0.7097074134746393
2216	76-77	-0.6333258508359663
2216	78-79	-0.6668307383187155
2216	80-81	-0.6204686898192548
2216	82-83	-0.7839778565670912
2216	84-85	-0.6396654723668576
2216	86-87	-0.5670065675995097
2216	88-89	-0.7050604161757335
2216	90-91	-0.6825140657921729
2216	92-93	-0.8211633014750426
2216	94-95	-0.7370519107237214
2216	96-97	-0.7249746265453609
2216	98-99	-0.6437648030762944
2216	100	-0.6122782648430878
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41468.0
3	8265.0
4	4639.0
5	3945.0
6	3328.0
7	3539.0
8	4174.0
9	4750.0
10	5292.0
11	5835.0
12	6625.0
13	7354.0
14	8193.0
15	8385.0
16	8442.0
17	8432.0
18	8321.0
19	8080.0
20	8099.0
21	8587.0
22	9691.0
23	14164.0
24	22216.0
25	29284.0
26	29523.0
27	25820.0
28	28448.0
29	35489.0
30	43195.0
31	53023.0
32	68712.0
33	93364.0
34	134402.0
35	208651.0
36	380573.0
37	777576.0
38	1197813.0
39	479386.0
40	788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.025697164182844	19.588940220863616	13.58375784305589	23.801604771897654
2	15.332334978379953	20.054738300610207	40.51919725939712	24.093729461612725
3	18.07702339207478	25.69532079468245	29.81479188456502	26.412863928677748
4	12.21717763929329	16.752559254818777	39.063701400508556	31.966561705379377
5	13.113655457977167	38.19050239342891	35.45940552728698	13.236436621306947
6	30.907974014741423	38.19295933472004	17.939853154985766	12.959213495552774
7	27.449848729220033	32.67949569378999	22.556107228318734	17.31454834867124
8	25.20136097059164	36.74441369752747	20.260844789627566	17.793380542253317
9	25.131851278662875	17.37250466958179	20.84677367280958	36.64887037894575
10-11	23.99520937124579	27.24381495985582	28.71134036921789	20.049635299680503
12-13	24.49924180862053	24.589597450853304	28.736402174334202	22.174758566191965
14-15	21.848423417664968	26.40888422405506	25.7593761095198	25.98331624876017
16-17	21.690976664694357	29.788696756703843	26.35678616068564	22.16354041791616
18-19	21.76543144799958	28.778148226240052	28.04840282536503	21.408017500395342
20-21	22.91512659367083	27.962586537278135	27.576550357718606	21.545736511332432
22-23	22.410136072003375	27.745737228150404	27.61977927192033	22.22434742792589
24-25	22.294377538809016	27.805462969205617	27.674914460044835	22.225245031940535
26-27	22.05269865002212	27.867954505347075	27.64604448857292	22.433302356057887
28-29	22.119113865351377	27.883376017031324	27.712614877113108	22.284895240504195
30-31	22.33062179945699	28.223962219379565	27.393453135163558	22.05196284599989
32-33	22.075794105634824	27.923072869320052	27.858740761234735	22.14239226381039
34-35	22.140018975044722	27.73773617577133	27.831531625842672	22.290713223341278
36-37	22.518417123209	27.937348060709326	27.358727413931366	22.185507402150307
38-39	22.35413670955721	28.153227041095292	27.474651575266357	22.01798467408114
40-41	22.07427766570966	27.926143962222138	27.77232882235366	22.227249549714546
42-43	22.007192258868617	27.873277115402225	27.717139617963127	22.40239100776603
44-45	22.135205904924394	27.998616650911856	27.654057329938635	22.21212011422511
46-47	22.236028821961355	28.092597823806997	27.589117348514474	22.08225600571717
48-49	22.225990335185504	27.8904917861725	27.594339187610657	22.28917869103134
50-51	22.217070955471712	27.85376884329425	27.589973922206124	22.339186279027913
52-53	22.232397771304424	27.855190122910265	27.63830204911931	22.274110056665993
54-55	22.311918120966286	28.177943828706404	27.464756261272	22.045381789055305
56-57	22.044599806786604	28.006219821934348	27.54102425232568	22.40815611895337
58-59	22.042769444509947	27.585273369989142	27.874913727722518	22.49704345777839
60-61	22.20432679894233	28.02935733503921	27.54045044826674	22.225865417751724
62-63	22.047195650369606	28.03004533280285	27.529250263156857	22.393508753670694
64-65	22.119229830854298	27.831658422532808	27.57762896707774	22.471482779535158
66-67	21.96358702095986	27.878443358320048	27.582792100860264	22.575177519859828
68-69	22.26639732137211	27.906549970279627	27.605776081210397	22.221276627137865
70-71	22.219351986557285	27.77371905085332	27.59840048982233	22.40852847276707
72-73	22.098711147163595	27.84266990471589	27.689299025397418	22.3693199227231
74-75	22.119497390552453	27.95714335771839	27.525265232579248	22.398094019149912
76-77	22.223343337309633	27.84944718195575	27.456726128503323	22.470483352231295
78-79	22.292067065626593	27.94214944940711	27.55490814099804	22.210875343968254
80-81	22.246684082516037	27.717121437748	27.70898163116982	22.32721284856614
82-83	22.13738587794947	27.79110343272384	27.753655488396014	22.31785520093068
84-85	22.121738351643938	27.826112152696147	27.555111263013504	22.497038232646414
86-87	22.292229072764027	27.834464171013686	27.548810055488467	22.324496700733818
88-89	22.03926815210989	27.77864704339058	27.609930490360636	22.572154314138896
90-91	22.063827467020264	27.722234251092214	27.6748211868965	22.53911709499102
92-93	22.223956351569218	27.77860533064337	27.613314267568956	22.384124050218453
94-95	22.067468358731603	27.614518751644656	27.685876071117093	22.63213681850665
96-97	22.1198178532189	27.639090804442983	27.681118800804683	22.559972541533433
98-99	21.959987450803542	27.760908831562865	27.619203536955723	22.659900180677873
100	22.107003781370704	27.57592378038018	27.55463809326608	22.76243434498303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	555.0
1	500.5
2	458.5
3	466.5
4	436.5
5	429.5
6	434.5
7	409.0
8	404.0
9	384.0
10	335.0
11	320.0
12	341.5
13	354.5
14	400.5
15	479.0
16	559.0
17	721.0
18	1014.0
19	1432.5
20	1983.0
21	2864.0
22	4479.0
23	7312.5
24	10943.5
25	15409.0
26	21626.0
27	30033.0
28	40364.5
29	50571.5
30	59624.5
31	69316.5
32	79298.5
33	89361.5
34	99229.5
35	107132.5
36	114132.5
37	121698.0
38	127252.5
39	128872.0
40	130730.5
41	133538.5
42	135110.5
43	136028.5
44	134772.0
45	133439.5
46	132647.5
47	129146.0
48	125841.5
49	123915.5
50	121548.0
51	117570.0
52	108945.5
53	103134.0
54	101086.5
55	96251.5
56	88027.5
57	79844.5
58	72383.5
59	64650.5
60	57915.5
61	50332.0
62	43729.0
63	37487.5
64	31342.5
65	26069.5
66	20778.0
67	16022.0
68	12583.5
69	10137.5
70	8006.0
71	5955.5
72	4620.0
73	3673.0
74	2801.0
75	1966.0
76	1266.0
77	891.0
78	651.5
79	473.5
80	331.5
81	178.0
82	96.0
83	63.0
84	44.0
85	33.0
86	25.0
87	19.0
88	19.0
89	16.5
90	12.0
91	9.5
92	6.0
93	5.0
94	5.0
95	2.5
96	2.0
97	2.0
98	1.0
99	3.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060981534127936415
2	0.04281346048878437
3	0.05637368936438336
4	0.07427845758847522
5	0.08728574509244785
6	0.08578490422660486
7	0.08823364458666448
8	0.10042468530395057
9	0.0956851878328674
10-11	0.09997706609834826
12-13	0.10463757194491335
14-15	0.10532216602406981
16-17	0.07444960610826434
18-19	0.09665941786859006
20-21	0.08973448545250748
22-23	0.05687396965299769
24-25	0.021459391327404223
26-27	0.18220734722164075
28-29	0.06611598972160983
30-31	0.07660871051175777
32-33	0.07164540343787348
34-35	0.020498326562434586
36-37	0.05126556431221598
38-39	0.07535800979022195
40-41	0.08046613484238933
42-43	0.07421263123471018
44-45	0.0719745352066987
46-47	0.07864932747847413
48-49	0.07551599303925804
50-51	0.0707764955681749
52-53	0.07441011029600532
54-55	0.0662476424291399
56-57	0.0680776150638081
58-59	0.06515492495664019
60-61	0.06221906957871923
62-63	0.06861739116468148
64-65	0.06437817398221267
66-67	0.06637929513666999
68-69	0.06569470105751353
70-71	0.05816416618679255
72-73	0.057137275068057866
74-75	0.05898041297347909
76-77	0.05389861846281772
78-79	0.05048881333778846
80-81	0.045077887058301874
82-83	0.050949597814143766
84-85	0.041431107059718454
86-87	0.03940365536375511
88-89	0.041154636373905276
90-91	0.03815295464221928
92-93	0.04048320756550183
94-95	0.0390745235949299
96-97	0.04191822207757978
98-99	0.042444832907700125
100	0.04984371507089103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3797871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.276804384703276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84484293045749	42.86873427731655
2	14.085261929569551	16.135175844945266
3	4.781758702876821	8.216515735185842
4	2.1457512048226284	4.916070880674682
5	1.16917711416503	3.3483364429551155
6	0.7071777068270014	2.4302927507491923
7	0.4462341687009828	1.7891207033319807
8	0.32385411292205096	1.4839462940014332
9	0.2346887121614535	1.2097997511992553
>10	1.1570818511181575	11.814704463948793
>50	0.07490032364313842	2.9454276664167693
>100	0.028857678028918518	2.6932745742653457
>500	4.135647068696598E-4	0.14860061500971905
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.633054150601745E-5	0.0
3	0.0	0.0	0.0	2.633054150601745E-5	0.0
4	0.0	0.0	0.0	2.633054150601745E-5	0.0
5	0.0	0.0	0.0	2.633054150601745E-5	0.0
6	0.0	0.0	0.0	2.633054150601745E-5	0.0
7	0.0	0.0	0.0	2.633054150601745E-5	0.0
8	0.0	0.0	0.0	7.899162451805235E-5	0.0
9	0.0	0.0	0.0	7.899162451805235E-5	0.0
10-11	0.0	0.0	0.0	7.899162451805235E-5	0.0
12-13	0.0	0.0	0.0	7.899162451805235E-5	0.0
14-15	0.0	0.0	0.0	1.1848743677707853E-4	0.0
16-17	0.0	0.0	0.0	1.8431379054212216E-4	0.0
18-19	0.0	2.633054150601745E-5	0.0	1.8431379054212216E-4	0.0
20-21	0.0	2.633054150601745E-5	0.0	1.8431379054212216E-4	0.0
22-23	0.0	2.633054150601745E-5	0.0	1.8431379054212216E-4	0.0
24-25	0.0	2.633054150601745E-5	0.0	2.106443320481396E-4	0.0
26-27	0.0	2.633054150601745E-5	0.0	3.4229703957822685E-4	0.0
28-29	0.0	2.633054150601745E-5	0.0	8.557425989455671E-4	0.0
30-31	0.0	2.633054150601745E-5	0.0	0.0032254913344871377	0.0
32-33	0.0	2.633054150601745E-5	0.0	0.008399442740419566	0.0
34-35	0.0	2.633054150601745E-5	0.0	0.016035299777164627	0.0
36-37	0.0	2.633054150601745E-5	0.0	0.02954286756975158	0.0
38-39	0.0	2.633054150601745E-5	0.0	0.054003940628841784	0.0
40-41	0.0	2.633054150601745E-5	0.0	0.0855479293530507	0.0
42-43	0.0	2.633054150601745E-5	0.0	0.11747371092909685	0.0
44-45	0.0	2.633054150601745E-5	0.0	0.15378352766589493	0.0
46-47	0.0	2.633054150601745E-5	0.0	0.19861127457988964	0.0
48-49	0.0	2.633054150601745E-5	0.0	0.25157515881924375	0.0
50-51	0.0	2.633054150601745E-5	0.0	0.30822531886944027	0.0
52-53	0.0	2.633054150601745E-5	0.0	0.36675811263731706	0.0
54-55	0.0	5.26610830120349E-5	0.0	0.43195253340621625	0.0
56-57	0.0	5.26610830120349E-5	0.0	0.5061256688286675	0.0
58-59	0.0	5.26610830120349E-5	0.0	0.5924635144268986	0.0
60-61	0.0	5.26610830120349E-5	0.0	0.688188198072025	0.0
62-63	0.0	5.26610830120349E-5	0.0	0.7852294087924524	0.0
64-65	0.0	5.26610830120349E-5	0.0	0.8884977925790529	0.0
66-67	0.0	5.26610830120349E-5	0.0	1.0068140808363422	0.0
68-69	0.0	5.26610830120349E-5	0.0	1.1455102082192892	0.0
70-71	0.0	5.26610830120349E-5	0.0	1.2957259475111187	0.0
72-73	0.0	5.26610830120349E-5	0.0	1.4576456125023731	0.0
74-75	0.0	5.26610830120349E-5	0.0	1.6136804014670325	0.0
76-77	0.0	5.26610830120349E-5	0.0	1.7917011925892163	0.0
78-79	0.0	5.26610830120349E-5	0.0	1.9971979037729295	0.0
80-81	0.0	5.26610830120349E-5	0.0	2.223258767872842	0.0
82-83	0.0	5.26610830120349E-5	0.0	2.4613921852532643	0.0
84-85	0.0	5.26610830120349E-5	0.0	2.700486667398656	0.0
86-87	0.0	6.582635376504363E-5	0.0	2.94838871567781	0.0
88	0.0	7.899162451805235E-5	0.0	3.153529964551192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4900	0.0	35.116684	1
GGTATCA	3140	0.0	35.036015	1
TCAACGC	4905	0.0	33.162586	4
ATCAACG	5185	0.0	31.462412	3
CAACGCA	5235	0.0	31.252129	5
AACGCAG	5530	0.0	29.574835	6
GTACATG	11800	0.0	28.726435	1
TACATGG	11690	0.0	27.909771	2
TATCAAC	6105	0.0	27.491209	2
ACATGGG	11905	0.0	26.023598	3
ACGCAGA	6685	0.0	24.113855	7
CGCAGAG	7055	0.0	22.800013	8
TATACCG	255	5.5842975E-10	22.123718	5
CATGGGG	8535	0.0	22.087786	4
TACACCG	535	0.0	18.453663	5
GAGTACA	8160	0.0	18.206457	1
GCAGAGT	8490	0.0	18.118908	9
ACCGTCC	445	0.0	17.967802	8
GACGCGT	155	1.2432974E-7	16.69961	10-11
AGTACAT	8310	0.0	16.689075	2
>>END_MODULE