FastQCFastQC Report
Wed 25 May 2016
SRR1033096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797871
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70580.18584096194947117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.14415971474544553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.053.8056871
GTATCAA98150.045.4215351
ATCAACG119150.036.7642943
TCAACGC119250.036.6935654
CAACGCA122200.035.8457455
AACGCAG127900.034.395236
TATCAAC129650.033.97122
ACGCAGA146850.029.7007167
CCGATCG1751.72804E-1029.5421339
CGCAGAG151600.028.5844848
GTACATG117250.026.2056961
GCAGAGT168900.025.4055359
TACATGG116700.025.2278922
ACATGGG120500.023.3248313
AGAGTAC156700.021.41477610-11
GTGGTAT23750.021.2315221
CAGAGTA167350.020.37492610-11
GAGTACT113000.020.17154112-13
AGTACTT117100.019.32480812-13
CATGGGG86800.019.2223854
Produced by FastQC (version 0.11.5)