##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3797871 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67075816951129 34.0 31.0 34.0 31.0 34.0 2 32.67160206336656 34.0 31.0 34.0 31.0 34.0 3 32.774485757941754 34.0 31.0 34.0 31.0 34.0 4 36.27496642197694 37.0 37.0 37.0 35.0 37.0 5 36.25772807975837 37.0 37.0 37.0 35.0 37.0 6 36.323800624086495 37.0 37.0 37.0 35.0 37.0 7 36.27430236572016 37.0 37.0 37.0 35.0 37.0 8 36.283075702149965 37.0 37.0 37.0 35.0 37.0 9 38.08310656154461 39.0 39.0 39.0 35.0 39.0 10-11 38.03934230520205 39.0 38.5 39.0 35.0 39.0 12-13 38.011258281284434 39.0 38.0 39.0 35.0 39.0 14-15 39.4895536209629 41.0 39.0 41.0 36.0 41.0 16-17 39.49270222711619 41.0 39.0 41.0 36.0 41.0 18-19 39.50561709441948 41.0 39.5 41.0 36.5 41.0 20-21 39.49751571340891 41.0 39.0 41.0 36.5 41.0 22-23 39.426989752943165 41.0 39.0 41.0 36.0 41.0 24-25 39.36709803992816 41.0 39.0 41.0 36.0 41.0 26-27 39.25431998611854 41.0 39.0 41.0 36.0 41.0 28-29 39.20265985864185 41.0 39.0 41.0 36.0 41.0 30-31 39.195890276420656 41.0 39.0 41.0 36.0 41.0 32-33 39.15836135561213 41.0 39.0 41.0 35.0 41.0 34-35 39.07315861965823 41.0 39.0 41.0 35.0 41.0 36-37 38.98616106234256 41.0 39.0 41.0 35.0 41.0 38-39 38.891813334365494 41.0 39.0 41.0 35.0 41.0 40-41 38.78967071288098 41.0 39.0 41.0 35.0 41.0 42-43 38.667613776244636 41.0 38.0 41.0 35.0 41.0 44-45 38.49072282865848 40.5 38.0 41.0 35.0 41.0 46-47 38.281553796850915 40.0 38.0 41.0 34.0 41.0 48-49 38.065685485367986 40.0 38.0 41.0 34.0 41.0 50-51 37.918476825568845 40.0 38.0 41.0 33.5 41.0 52-53 37.77951805103438 40.0 37.5 41.0 33.0 41.0 54-55 37.622454922771205 40.0 37.0 41.0 33.0 41.0 56-57 37.46574870499814 40.0 37.0 41.0 33.0 41.0 58-59 37.27698518459421 40.0 36.0 41.0 33.0 41.0 60-61 37.07447080219418 39.5 36.0 41.0 32.5 41.0 62-63 36.83837365724112 39.0 35.0 41.0 32.0 41.0 64-65 36.567323640007785 39.0 35.0 41.0 32.0 41.0 66-67 36.279810451697806 38.5 35.0 41.0 31.5 41.0 68-69 35.971529180427666 37.5 35.0 40.0 31.0 41.0 70-71 35.642198879319494 37.0 35.0 40.0 31.0 41.0 72-73 35.30264679869327 36.5 35.0 39.0 31.0 41.0 74-75 34.959092475758126 36.0 35.0 39.0 30.5 41.0 76-77 34.611504313864266 35.5 35.0 38.5 30.0 40.0 78-79 34.25866663191035 35.0 34.0 37.0 30.0 39.0 80-81 33.93623651250925 35.0 34.0 37.0 30.0 39.0 82-83 33.64796645278368 35.0 34.0 36.5 30.0 39.0 84-85 33.432631071460825 35.0 34.0 36.0 30.0 37.0 86-87 33.22855778935093 35.0 34.0 36.0 30.0 37.0 88-89 33.02656040713337 35.0 34.0 35.5 29.0 37.0 90-91 32.84890547888541 35.0 34.0 35.0 29.0 36.0 92-93 32.67601585203921 35.0 34.0 35.0 29.0 36.0 94-95 32.52358150132008 35.0 34.0 35.0 29.0 36.0 96-97 32.379826355344875 35.0 34.0 35.0 29.0 36.0 98-99 32.254934145999165 35.0 34.0 35.0 29.0 35.5 100 32.15162600309489 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17737986384467064 1101 2 0.1836735180233262 1101 3 0.19039138058487737 1101 4 0.15098081304496702 1101 5 0.14677491959305655 1101 6 0.15635286007245242 1101 7 0.1620476697695281 1101 8 0.15327077503609843 1101 9 0.19850221371804366 1101 10-11 0.2304416694784166 1101 12-13 0.2188070851415702 1101 14-15 0.30354980233556716 1101 16-17 0.32470029286617574 1101 18-19 0.2854266071674516 1101 20-21 0.27876326005296903 1101 22-23 0.2905320054811611 1101 24-25 0.3527018226375205 1101 26-27 0.3060955721451677 1101 28-29 0.34929490800124796 1101 30-31 0.3311026768365366 1101 32-33 0.3124627760620058 1101 34-35 0.3080696297882781 1101 36-37 0.273157674449088 1101 38-39 0.24633539062384813 1101 40-41 0.3010169470816493 1101 42-43 0.3126850929735454 1101 44-45 0.2608121709375908 1101 46-47 0.24191523053745811 1101 48-49 0.2114623055208824 1101 50-51 0.2818161356965234 1101 52-53 0.27546795175364736 1101 54-55 0.31416362549523313 1101 56-57 0.24454265140175124 1101 58-59 0.2411198271021462 1101 60-61 0.2615537074289662 1101 62-63 0.31208785729994304 1101 64-65 0.3104748755992688 1101 66-67 0.2707517505110886 1101 68-69 0.23520319597854922 1101 70-71 0.26639045289972785 1101 72-73 0.28417231702908907 1101 74-75 0.2864080010108978 1101 76-77 0.3202021948211069 1101 78-79 0.2611343069292502 1101 80-81 0.27685573256619733 1101 82-83 0.3208710806164845 1101 84-85 0.2907248263203499 1101 86-87 0.3221953861288611 1101 88-89 0.3530349851998196 1101 90-91 0.3462509977062638 1101 92-93 0.3077901679758881 1101 94-95 0.3371983922152637 1101 96-97 0.31564799321840553 1101 98-99 0.30381233516947503 1101 100 0.33325086253190506 1102 1 0.10783472479746337 1102 2 0.08855805257591243 1102 3 0.10137689115545356 1102 4 0.06783355458650675 1102 5 0.08558012084639444 1102 6 0.10299888348196617 1102 7 0.11126005259186655 1102 8 0.11989889104180662 1102 9 0.16431245234985425 1102 10-11 0.16557148894433027 1102 12-13 0.19144187312905103 1102 14-15 0.24712426613824334 1102 16-17 0.25801432783996603 1102 18-19 0.24518895178457 1102 20-21 0.26291845478908016 1102 22-23 0.24688455104532636 1102 24-25 0.2674968017478534 1102 26-27 0.2947412625543464 1102 28-29 0.2717680664702584 1102 30-31 0.267006330582511 1102 32-33 0.3353119301352194 1102 34-35 0.275660303291815 1102 36-37 0.24317209985770916 1102 38-39 0.2444529945505849 1102 40-41 0.2682478489598523 1102 42-43 0.2538025278669096 1102 44-45 0.2977457882653951 1102 46-47 0.3181646496291961 1102 48-49 0.33727183485738266 1102 50-51 0.27807903600506023 1102 52-53 0.2353598924547171 1102 54-55 0.23215354244780428 1102 56-57 0.23047689926546155 1102 58-59 0.24243012353692706 1102 60-61 0.19784304050253354 1102 62-63 0.204206112488734 1102 64-65 0.22662459724691075 1102 66-67 0.21510171820130353 1102 68-69 0.245521945923862 1102 70-71 0.21488594081431245 1102 72-73 0.20680443364460643 1102 74-75 0.24946724467179848 1102 76-77 0.21177656098053887 1102 78-79 0.2305355258117956 1102 80-81 0.22189639959856322 1102 82-83 0.28264662641691984 1102 84-85 0.26058639628565317 1102 86-87 0.23867125696158098 1102 88-89 0.21696617848785138 1102 90-91 0.18893096642357676 1102 92-93 0.2648024387848409 1102 94-95 0.26939597793982273 1102 96-97 0.29492453732897417 1102 98-99 0.2909894618694224 1102 100 0.2778080342675011 1103 1 0.11673528495325058 1103 2 0.14995167986911895 1103 3 0.1725082237930593 1103 4 0.10835219447752564 1103 5 0.1432399180459356 1103 6 0.14596637624025277 1103 7 0.14929082702431629 1103 8 0.14702239515276005 1103 9 0.16885335542632163 1103 10-11 0.2126592802789844 1103 12-13 0.19229446080172607 1103 14-15 0.27811773803129825 1103 16-17 0.30197745443907564 1103 18-19 0.2599487154983109 1103 20-21 0.3017701191637556 1103 22-23 0.27705428832803136 1103 24-25 0.2544023880258237 1103 26-27 0.3004139371600729 1103 28-29 0.27443839706945994 1103 30-31 0.2530788354834641 1103 32-33 0.21326550840260694 1103 34-35 0.2434956408970308 1103 36-37 0.2719585156734112 1103 38-39 0.2658993538630554 1103 40-41 0.2287549151154593 1103 42-43 0.18422926059490408 1103 44-45 0.2105449254369205 1103 46-47 0.21552699207521897 1103 48-49 0.1798170163147006 1103 50-51 0.22303105051773997 1103 52-53 0.17751031525367722 1103 54-55 0.19153818384081234 1103 56-57 0.23351541119519226 1103 58-59 0.2702879584031308 1103 60-61 0.24483248759658238 1103 62-63 0.1664151029404053 1103 64-65 0.20297199461941773 1103 66-67 0.1808027419567182 1103 68-69 0.15006707536438313 1103 70-71 0.18729672389454777 1103 72-73 0.20217006290145179 1103 74-75 0.2437872552051985 1103 76-77 0.2816249482975479 1103 78-79 0.275092375284963 1103 80-81 0.2600694074368448 1103 82-83 0.2635800351745985 1103 84-85 0.27514906513702186 1103 86-87 0.27416458743399374 1103 88-89 0.21986408253638956 1103 90-91 0.18340215074832145 1103 92-93 0.26715091550570236 1103 94-95 0.2540941017592857 1103 96-97 0.2951191939043838 1103 98-99 0.27946652409125505 1103 100 0.30784529129220317 1104 1 0.09203415077770671 1104 2 0.08083558252467071 1104 3 0.10965694053138719 1104 4 0.05525562077568935 1104 5 0.09693703288823485 1104 6 0.11539910821095845 1104 7 0.11045112027608894 1104 8 0.1332648290652756 1104 9 0.19814255124845204 1104 10-11 0.220575110728781 1104 12-13 0.18395248892733918 1104 14-15 0.2139156456008564 1104 16-17 0.23440045148635846 1104 18-19 0.19464729507853207 1104 20-21 0.19644583694660156 1104 22-23 0.185918325695539 1104 24-25 0.22176120121773124 1104 26-27 0.1952647024613441 1104 28-29 0.26185307196622176 1104 30-31 0.23956764560958277 1104 32-33 0.21860111308193808 1104 34-35 0.22670547865206458 1104 36-37 0.2574297476769942 1104 38-39 0.2710261478360607 1104 40-41 0.268528760199672 1104 42-43 0.2942735238173668 1104 44-45 0.2695196384639047 1104 46-47 0.22738871467517896 1104 48-49 0.1876505687977641 1104 50-51 0.2727925324308913 1104 52-53 0.27338291164212336 1104 54-55 0.2845565058096753 1104 56-57 0.2769530022484119 1104 58-59 0.20508205365460697 1104 60-61 0.2928649351819601 1104 62-63 0.2656747561491528 1104 64-65 0.23489626818981435 1104 66-67 0.24097701633687052 1104 68-69 0.2761368121551655 1104 70-71 0.27536643280164697 1104 72-73 0.30233748465711585 1104 74-75 0.3225465626141286 1104 76-77 0.2916926802802209 1104 78-79 0.23479842219797575 1104 80-81 0.24648203034857374 1104 82-83 0.25733606343512605 1104 84-85 0.2607528502192267 1104 86-87 0.26639243536768475 1104 88-89 0.24916341787687202 1104 90-91 0.2569295018878677 1104 92-93 0.2507410908193819 1104 94-95 0.2638944039451019 1104 96-97 0.31033628732976126 1104 98-99 0.30243429351973106 1104 100 0.28676720418557267 1105 1 0.04376646900090009 1105 2 0.04780073123947659 1105 3 0.06026586835581327 1105 4 0.02058898465566017 1105 5 0.05140796169678197 1105 6 0.09332183853521059 1105 7 0.0983551532536282 1105 8 0.08490483945024607 1105 9 0.08049838220166805 1105 10-11 0.10880391100139519 1105 12-13 0.14034261257642555 1105 14-15 0.1552117010838785 1105 16-17 0.17503490623524698 1105 18-19 0.13697252952340477 1105 20-21 0.16719397060166585 1105 22-23 0.2156327331256378 1105 24-25 0.20366786303167572 1105 26-27 0.2522825260896582 1105 28-29 0.18712984352696793 1105 30-31 0.20210686057750138 1105 32-33 0.22450971643294793 1105 34-35 0.2512755609068904 1105 36-37 0.2510104789300769 1105 38-39 0.23161068421594422 1105 40-41 0.1930944708568063 1105 42-43 0.20453495938592425 1105 44-45 0.2543604471847658 1105 46-47 0.2579672223246092 1105 48-49 0.3111504578880897 1105 50-51 0.25806245237220793 1105 52-53 0.274711957492336 1105 54-55 0.30862682661548746 1105 56-57 0.27004886687729623 1105 58-59 0.28530683259170075 1105 60-61 0.20342805774639316 1105 62-63 0.24588681437148807 1105 64-65 0.20880223829610856 1105 66-67 0.19742030716340508 1105 68-69 0.23071018463004833 1105 70-71 0.23692385839615326 1105 72-73 0.19103897854125762 1105 74-75 0.17885922649477948 1105 76-77 0.17410630382173053 1105 78-79 0.20335229100182772 1105 80-81 0.21773784041565136 1105 82-83 0.28483678596771256 1105 84-85 0.26994357497802923 1105 86-87 0.20165725566830162 1105 88-89 0.2181243729303759 1105 90-91 0.25945340657888494 1105 92-93 0.2350557882777693 1105 94-95 0.19082329878983728 1105 96-97 0.24613947539670278 1105 98-99 0.2856598820539986 1105 100 0.2758640254228979 1106 1 0.05016049644633824 1106 2 0.06306895503498566 1106 3 0.07939795934045435 1106 4 0.05268953825264333 1106 5 0.05362170086576157 1106 6 0.06960243681211864 1106 7 0.06976693732826078 1106 8 0.04928221704067681 1106 9 0.05202608133794229 1106 10-11 0.1267888880913972 1106 12-13 0.06855679651578583 1106 14-15 0.14220692159901205 1106 16-17 0.15007963370749167 1106 18-19 0.14565221435783116 1106 20-21 0.1464906819625682 1106 22-23 0.1348659802747747 1106 24-25 0.09786717759681096 1106 26-27 0.0914083051891339 1106 28-29 0.06267365450699458 1106 30-31 0.06749484259931648 1106 32-33 0.048566028701323205 1106 34-35 0.04372603361296967 1106 36-37 0.03599610116928176 1106 38-39 0.019854982538504373 1106 40-41 0.05631369638582839 1106 42-43 0.010391112324349194 1106 44-45 -0.0027904388308499506 1106 46-47 -0.0743102663121391 1106 48-49 -0.10358622300226017 1106 50-51 -0.0938938043046349 1106 52-53 -0.0751594181306956 1106 54-55 -0.07138822103578235 1106 56-57 -0.04562335191410938 1106 58-59 -0.0426680924840781 1106 60-61 -0.07865084724201665 1106 62-63 -0.05238842993341564 1106 64-65 0.02714007419020703 1106 66-67 0.025526523139589585 1106 68-69 -0.04848378687087518 1106 70-71 -0.049638447189721546 1106 72-73 -0.06976151465586611 1106 74-75 0.005425568914105838 1106 76-77 -0.04249730282532482 1106 78-79 -0.05012893722971512 1106 80-81 -0.03315996069312632 1106 82-83 -0.0074233570692427975 1106 84-85 -0.011548726655924213 1106 86-87 -0.015735479729663382 1106 88-89 -0.023940477873580335 1106 90-91 -0.06708159584228923 1106 92-93 -0.013312631701985822 1106 94-95 0.09553003543295091 1106 96-97 0.019957532126646527 1106 98-99 0.025856385591445985 1106 100 0.03068264951652111 1107 1 0.023770600869518432 1107 2 0.07567535692299998 1107 3 0.032127986102373995 1107 4 0.046589092943833066 1107 5 0.02861096902290683 1107 6 0.05793732549160069 1107 7 0.04481175247696001 1107 8 0.026394395192888 1107 9 0.0703820526532084 1107 10-11 0.10731931810381212 1107 12-13 0.13376660962720877 1107 14-15 0.1488822387676194 1107 16-17 0.11689720404744008 1107 18-19 0.10996111482427295 1107 20-21 0.11504376153427387 1107 22-23 0.12615616338020175 1107 24-25 0.13717471141887216 1107 26-27 0.12649124417072954 1107 28-29 0.15338723978482705 1107 30-31 0.1693622060464719 1107 32-33 0.15582372933603494 1107 34-35 0.2126342857536443 1107 36-37 0.20687570935513833 1107 38-39 0.1734683948640381 1107 40-41 0.22922485678223836 1107 42-43 0.24556961461762228 1107 44-45 0.26856916464385705 1107 46-47 0.28729422190909304 1107 48-49 0.25723308661488176 1107 50-51 0.2878237086742317 1107 52-53 0.3004904824301633 1107 54-55 0.3489276444399749 1107 56-57 0.35036712463318764 1107 58-59 0.3512407544773595 1107 60-61 0.2988687960490637 1107 62-63 0.32018462917402246 1107 64-65 0.342051253851821 1107 66-67 0.3670839106721999 1107 68-69 0.3378856195235258 1107 70-71 0.3462984575052275 1107 72-73 0.3802298752565747 1107 74-75 0.34599433850539185 1107 76-77 0.3030556849479922 1107 78-79 0.3098820086878433 1107 80-81 0.3660165825051678 1107 82-83 0.3380028419610426 1107 84-85 0.3394372916983528 1107 86-87 0.3424706447637291 1107 88-89 0.3047588446506353 1107 90-91 0.3214934636861244 1107 92-93 0.4200882574692102 1107 94-95 0.3856657527167471 1107 96-97 0.368797926385092 1107 98-99 0.3903669872726354 1107 100 0.3993045490151772 1108 1 -0.038459639971478055 1108 2 -0.051849675289446395 1108 3 -0.008437550523396453 1108 4 -0.013572685124820794 1108 5 -0.004321634648583483 1108 6 -0.03931829086911165 1108 7 -0.06182424540768494 1108 8 -0.03811225035916266 1108 9 -0.03282344376908952 1108 10-11 -0.011952578325498564 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-0.1396360616723058 2214 60-61 -0.18113913162713402 2214 62-63 -0.17210371717516182 2214 64-65 -0.18738526006592338 2214 66-67 -0.1530001993786314 2214 68-69 -0.1312765727005214 2214 70-71 -0.11467854179326764 2214 72-73 -0.1491752276438021 2214 74-75 -0.18699054823868266 2214 76-77 -0.15234534184638449 2214 78-79 -0.13057769952843756 2214 80-81 -0.13197602246577134 2214 82-83 -0.11460399551162226 2214 84-85 -0.04644122365844794 2214 86-87 -0.10929340486359251 2214 88-89 -0.1017767733626016 2214 90-91 -0.08648288745540356 2214 92-93 -0.09467549504893924 2214 94-95 -0.15360983800246686 2214 96-97 -0.1226168592772865 2214 98-99 -0.1116472234749537 2214 100 -0.12786067137898982 2215 1 -0.162820131999176 2215 2 -0.15032769851205074 2215 3 -0.15900708538192276 2215 4 -0.11051464831137991 2215 5 -0.08680885630725754 2215 6 -0.13461605822943312 2215 7 -0.13149964106846568 2215 8 -0.0916426180011527 2215 9 -0.19182185817143704 2215 10-11 -0.18404674903823093 2215 12-13 -0.14579424119887108 2215 14-15 -0.2545252238900986 2215 16-17 -0.24566812986631703 2215 18-19 -0.2969040833357752 2215 20-21 -0.2760379645421196 2215 22-23 -0.32643304587773514 2215 24-25 -0.3377939143795814 2215 26-27 -0.27469307997703396 2215 28-29 -0.3314059884760212 2215 30-31 -0.3640744567301226 2215 32-33 -0.2886924388575238 2215 34-35 -0.2824420630615663 2215 36-37 -0.29918939783500065 2215 38-39 -0.28087983808904937 2215 40-41 -0.24396502532449205 2215 42-43 -0.26817596171761693 2215 44-45 -0.30005901523376366 2215 46-47 -0.2742961854231538 2215 48-49 -0.2184805669147707 2215 50-51 -0.20220970488797718 2215 52-53 -0.17109441732614528 2215 54-55 -0.16158482906511296 2215 56-57 -0.13105815358277795 2215 58-59 -0.13905897294345237 2215 60-61 -0.13155528729788557 2215 62-63 -0.13375028517132392 2215 64-65 -0.11438658506293109 2215 66-67 -0.12689053481651769 2215 68-69 -0.07973341237914866 2215 70-71 -0.08817026600424072 2215 72-73 -0.13152161502426907 2215 74-75 -0.15548667706016062 2215 76-77 -0.08644427203111604 2215 78-79 -0.09567686177479118 2215 80-81 -0.12183750224109247 2215 82-83 -0.13176189585040277 2215 84-85 -0.12229563549237099 2215 86-87 -0.1318794993387442 2215 88-89 -0.11130524987072477 2215 90-91 -0.1582065464605975 2215 92-93 -0.16857583959634326 2215 94-95 -0.13751703770358148 2215 96-97 -0.15399020117030915 2215 98-99 -0.15535527093667412 2215 100 -0.20288670194728198 2216 1 -0.2006384794974707 2216 2 -0.1951096724567165 2216 3 -0.1551926612358301 2216 4 -0.18879151331989874 2216 5 -0.19494908377752296 2216 6 -0.19771307865061516 2216 7 -0.1828846297143727 2216 8 -0.1620478530219316 2216 9 -0.2228739607418504 2216 10-11 -0.2942423663430631 2216 12-13 -0.30948587445607956 2216 14-15 -0.37956706326896494 2216 16-17 -0.3814699730649451 2216 18-19 -0.3299793262388562 2216 20-21 -0.3205328895954196 2216 22-23 -0.3404018549040302 2216 24-25 -0.359315135927595 2216 26-27 -0.4081109687797806 2216 28-29 -0.3557370035139158 2216 30-31 -0.32203130309300576 2216 32-33 -0.3425118400283793 2216 34-35 -0.33612684178223873 2216 36-37 -0.3533935675296931 2216 38-39 -0.3922621318581392 2216 40-41 -0.39893322901509976 2216 42-43 -0.36771633550431915 2216 44-45 -0.34359422424453356 2216 46-47 -0.26560593602161475 2216 48-49 -0.33835945121958133 2216 50-51 -0.3755367771511402 2216 52-53 -0.3467845647593819 2216 54-55 -0.25605725634795107 2216 56-57 -0.2624881053987096 2216 58-59 -0.35256071045112236 2216 60-61 -0.37103353324869204 2216 62-63 -0.3987285804877416 2216 64-65 -0.43584496661540584 2216 66-67 -0.4423875736891105 2216 68-69 -0.4398468480800304 2216 70-71 -0.43320250741341226 2216 72-73 -0.38733813842274856 2216 74-75 -0.5307283257744686 2216 76-77 -0.43597343739619987 2216 78-79 -0.5162993864130883 2216 80-81 -0.41375922456680314 2216 82-83 -0.37289951422671663 2216 84-85 -0.45721395563726475 2216 86-87 -0.4606325806431002 2216 88-89 -0.5852015238191228 2216 90-91 -0.4948956758292553 2216 92-93 -0.49392669961003577 2216 94-95 -0.39491544570307724 2216 96-97 -0.4462963278473211 2216 98-99 -0.5269632060136118 2216 100 -0.5146460761531841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 7.0 4 19.0 5 29.0 6 95.0 7 229.0 8 493.0 9 948.0 10 1670.0 11 2608.0 12 4011.0 13 6389.0 14 7437.0 15 9789.0 16 12040.0 17 17653.0 18 20964.0 19 11040.0 20 7686.0 21 7431.0 22 7901.0 23 8540.0 24 9611.0 25 11674.0 26 14787.0 27 18926.0 28 24610.0 29 31797.0 30 40827.0 31 51861.0 32 66641.0 33 86877.0 34 121766.0 35 186155.0 36 343252.0 37 758590.0 38 1306192.0 39 595340.0 40 1963.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73644697328908 19.815315315315317 13.814577735630367 23.63365997576524 2 15.598759042053103 20.300468289005742 40.007907754825816 24.092864914115342 3 18.133263538227435 25.785254186455624 30.14548224582605 25.93600002949089 4 12.283583865925742 16.988890340030586 38.70436276389129 32.02316303015238 5 12.771853826063579 37.83292112740362 35.584233116605965 13.810991929926836 6 30.680020822203186 38.388975501787975 17.615852468845027 13.315151207163806 7 27.067901990351963 32.69358016636163 22.666041053000484 17.572476790285926 8 25.44797572950255 35.906570591559145 20.262847886521417 18.382605792416893 9 25.38145713743305 16.339154226144068 21.13141810240527 37.14797053401761 10-11 24.06694528059048 26.676831194929342 28.766659434500802 20.48956408997938 12-13 24.981285567624596 23.967243753144853 29.128372185363855 21.923098493866693 14-15 22.07038228644086 25.627873493987007 26.688102428236544 25.61364179133559 16-17 21.289055994493747 29.211560865323133 27.129311748747227 22.370071391435896 18-19 21.2046648461644 27.756169077634123 29.784670444064055 21.25449563213742 20-21 22.29920688448961 26.96509794106987 29.521701700992704 21.213993473447818 22-23 21.81539580385406 26.79841784833904 29.569210709382588 21.816975638424317 24-25 21.67248184025427 26.910706475788892 29.60062909724861 21.816182586708226 26-27 21.468112462192554 26.972721238233184 29.595696316586462 21.963469982987803 28-29 21.573898475787367 26.995803560555444 29.64911183593403 21.78118612772316 30-31 21.796206552892215 27.338517825146496 29.255713407401263 21.60956221456002 32-33 21.527100314886948 27.055460809490366 29.686145211356575 21.73129366426611 34-35 21.614228076730356 26.8864845593755 29.655180494545498 21.844106869348643 36-37 22.00784334170381 27.12465747256818 29.097499625448048 21.769999560279956 38-39 21.847569335556685 27.34092337522786 29.161653989827457 21.649853299388 40-41 21.641618618869668 27.17925256739473 29.281432277455703 21.8976965362799 42-43 21.693917460598318 27.146735631620977 29.104227078802836 22.05511982897787 44-45 21.79876567687528 27.269212145436217 29.006619761440028 21.92540241624847 46-47 21.9900570609165 27.49364051596276 28.59632409842251 21.919978324698235 48-49 22.045311702266876 27.452064590924756 28.340233251734986 22.162390455073382 50-51 22.10851816715207 27.478368801889268 28.216492871927457 22.196620159031205 52-53 22.094971103547223 27.54538003002208 28.164898175846414 22.19475069058428 54-55 22.120445902454296 27.869456334878144 28.041315779287924 21.968781983379635 56-57 21.88333937619261 27.61906605042667 28.15183822725943 22.345756346121288 58-59 21.849544126169633 27.185125561136754 28.52591623043542 22.439414082258192 60-61 21.976299352979602 27.595671364298575 28.238689518417033 22.18933976430479 62-63 21.87330743987882 27.533978905549976 28.26858784829711 22.324125806274093 64-65 21.919280565348323 27.34959928865409 28.293272730959 22.437847415038583 66-67 21.782862029805646 27.42909119346076 28.239966549680073 22.54808022705353 68-69 22.04027633434908 27.510194548881834 28.200003528299995 22.249525588469094 70-71 22.040741794938164 27.380617920207357 28.15364353009543 22.42499675475905 72-73 21.929810472213298 27.452552226780348 28.184266929270695 22.433370371735663 74-75 21.93658066744886 27.62689764562396 27.982439602892573 22.454082084034603 76-77 22.04611478378281 27.507477215524172 27.93185971824741 22.514548282445613 78-79 22.076276419077953 27.594920943865652 28.028979920592352 22.29982271646404 80-81 22.00053661643589 27.415544129855913 28.13826483311308 22.44565442059512 82-83 21.87271294170711 27.460787503082322 28.224374513624696 22.442125041585868 84-85 21.832416108919972 27.431803239235876 28.054151918272108 22.681628733572047 86-87 21.968895081856708 27.50000263306455 27.9845655022202 22.54653678285854 88-89 21.695422955212234 27.44858967533121 28.073468222734448 22.78251914672211 90-91 21.673108576126044 27.410831623785114 28.104182778023095 22.811877022065747 92-93 21.78703115041643 27.39202334796838 28.057176613114827 22.763768888500366 94-95 21.59213945145835 27.256959986855726 28.133376445422307 23.017524116263612 96-97 21.613607866741866 27.247672506268 28.122204018998058 23.016515607992076 98-99 21.468193313875034 27.328749178814938 28.082975566535406 23.12008194077462 100 21.597416025978767 27.175857210526633 28.004584673887027 23.222142089607573 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 61.0 1 47.0 2 115.5 3 225.5 4 181.0 5 149.0 6 352.5 7 782.0 8 1329.0 9 1634.5 10 1427.0 11 1207.5 12 1313.5 13 1557.5 14 1886.5 15 2171.5 16 2420.0 17 2824.5 18 3253.5 19 3708.5 20 4414.0 21 5455.5 22 7101.0 23 9887.5 24 13417.5 25 17382.0 26 23157.5 27 31150.0 28 40853.5 29 50590.5 30 59389.0 31 68947.0 32 79248.5 33 89211.0 34 98629.0 35 106657.0 36 113501.5 37 120295.0 38 125611.5 39 128198.0 40 129943.0 41 132168.0 42 134568.0 43 135959.5 44 134480.0 45 133026.0 46 131993.5 47 128121.5 48 124765.0 49 122668.5 50 120350.5 51 116469.0 52 107649.5 53 101803.5 54 99753.0 55 95015.5 56 86867.5 57 78587.0 58 71151.0 59 63544.0 60 57098.0 61 49863.0 62 43153.5 63 37197.5 64 31030.5 65 25262.0 66 20004.5 67 15439.0 68 12163.0 69 9783.0 70 7558.5 71 5604.5 72 4275.5 73 3353.5 74 2612.5 75 1789.0 76 1066.0 77 692.0 78 518.0 79 375.0 80 247.0 81 102.0 82 38.5 83 23.5 84 10.5 85 3.5 86 2.5 87 1.5 88 2.5 89 1.5 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04399833485655516 2 0.24184602373277028 3 0.0023170876525295354 4 0.002501401443071658 5 3.159664980722094E-4 6 5.2661083012034905E-5 7 0.0 8 2.3697487355415705E-4 9 0.0 10-11 6.056024546384014E-4 12-13 0.0 14-15 3.159664980722094E-4 16-17 1.7114851978911343E-4 18-19 4.739497471083141E-4 20-21 1.3165270753008726E-5 22-23 1.3165270753008726E-4 24-25 0.0012638659922888376 26-27 2.1064433204813962E-4 28-29 2.2380960280114835E-4 30-31 1.579832490361047E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.215689527106108E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.1064433204813962E-4 70-71 5.2661083012034905E-5 72-73 2.896359565661919E-4 74-75 1.8431379054212216E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7114851978911343E-4 84-85 0.0 86-87 3.949581225902617E-4 88-89 0.0024487403600596228 90-91 0.0022117654865054658 92-93 6.450982668974275E-4 94-95 5.002802886143315E-4 96-97 2.3697487355415705E-4 98-99 1.579832490361047E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3797871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.08050754951511 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17190660781505 40.303457543241706 2 14.983757252252946 16.506259089056403 3 5.161117042087962 8.528308386019715 4 2.276713139660584 5.016100611086202 5 1.2297153860757353 3.386667380324971 6 0.7226265950357229 2.3881583774007322 7 0.4789876663146726 1.8468018639398964 8 0.3510101240278896 1.5467052629179527 9 0.23713912996700348 1.1755569274589681 >10 1.2719328141651116 12.42167284531282 >50 0.08028736586646741 3.056075004195004 >100 0.033753623987726064 3.0629915564629666 >500 8.617522505066312E-4 0.3088174501980947 >1k 9.575024635907952E-5 0.12193423651142576 >5k 9.575024635907952E-5 0.33049346587320483 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7058 0.18584096194947117 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5475 0.14415971474544553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.633054150601745E-5 0.0 8 0.0 0.0 0.0 7.899162451805235E-5 0.0 9 0.0 0.0 0.0 7.899162451805235E-5 0.0 10-11 0.0 0.0 0.0 7.899162451805235E-5 0.0 12-13 0.0 0.0 0.0 7.899162451805235E-5 0.0 14-15 0.0 0.0 0.0 7.899162451805235E-5 0.0 16-17 0.0 0.0 0.0 1.053221660240698E-4 0.0 18-19 0.0 0.0 0.0 1.053221660240698E-4 0.0 20-21 0.0 0.0 0.0 1.053221660240698E-4 0.0 22-23 0.0 0.0 0.0 1.053221660240698E-4 0.0 24-25 0.0 0.0 0.0 1.3165270753008726E-4 0.0 26-27 0.0 0.0 0.0 3.028012273192007E-4 0.0 28-29 0.0 0.0 0.0 8.294120574395497E-4 0.0 30-31 0.0 0.0 0.0 0.0031991607929811204 0.0 32-33 0.0 0.0 0.0 0.008386277469666558 0.0 34-35 0.0 0.0 0.0 0.01608796086017666 0.0 36-37 0.0 0.0 0.0 0.029569198111257595 0.0 38-39 0.0 0.0 0.0 0.05403027117034781 0.0 40-41 0.0 0.0 0.0 0.08574540841434583 0.0 42-43 0.0 0.0 0.0 0.11790816486394615 0.0 44-45 0.0 0.0 0.0 0.1542179816007442 0.0 46-47 0.0 0.0 0.0 0.19908522432699793 0.0 48-49 0.0 0.0 0.0 0.2523519097936713 0.0 50-51 0.0 0.0 0.0 0.30947601959097615 0.0 52-53 0.0 0.0 0.0 0.3684827631059612 0.0 54-55 0.0 0.0 0.0 0.4340721419974507 0.0 56-57 0.0 0.0 0.0 0.5086270702717391 0.0 58-59 0.0 0.0 0.0 0.5950570727652413 0.0 60-61 0.0 0.0 0.0 0.691189879803711 0.0 62-63 0.0 0.0 0.0 0.789007841498566 0.0 64-65 0.0 0.0 0.0 0.8928818277398047 0.0 66-67 0.0 0.0 0.0 1.0118695448054975 0.0 68-69 0.0 0.0 0.0 1.1518690339929925 0.0 70-71 0.0 0.0 0.0 1.3027693673639784 0.0 72-73 0.0 0.0 0.0 1.4657027582032143 0.0 74-75 0.0 0.0 0.0 1.623291049116729 0.0 76-77 0.0 0.0 0.0 1.8028126811047558 0.0 78-79 0.0 0.0 0.0 2.0091783001581676 0.0 80-81 0.0 0.0 0.0 2.2368848231022067 0.0 82-83 0.0 0.0 0.0 2.4762426106626583 0.0 84-85 0.0 0.0 0.0 2.7161270090532303 0.0 86-87 0.0 0.0 0.0 2.965003287368107 0.0 88 0.0 0.0 0.0 3.171803360356368 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5115 0.0 53.805687 1 GTATCAA 9815 0.0 45.421535 1 ATCAACG 11915 0.0 36.764294 3 TCAACGC 11925 0.0 36.693565 4 CAACGCA 12220 0.0 35.845745 5 AACGCAG 12790 0.0 34.39523 6 TATCAAC 12965 0.0 33.9712 2 ACGCAGA 14685 0.0 29.700716 7 CCGATCG 175 1.72804E-10 29.542133 9 CGCAGAG 15160 0.0 28.584484 8 GTACATG 11725 0.0 26.205696 1 GCAGAGT 16890 0.0 25.405535 9 TACATGG 11670 0.0 25.227892 2 ACATGGG 12050 0.0 23.324831 3 AGAGTAC 15670 0.0 21.414776 10-11 GTGGTAT 2375 0.0 21.231522 1 CAGAGTA 16735 0.0 20.374926 10-11 GAGTACT 11300 0.0 20.171541 12-13 AGTACTT 11710 0.0 19.324808 12-13 CATGGGG 8680 0.0 19.222385 4 >>END_MODULE