FastQCFastQC Report
Wed 25 May 2016
SRR1033095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013500
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14170.13981253083374445No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11040.10892945239269858No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAC700.00884615726.8505256
CCTCGCG1606.828668E-826.4715129
GTACATG41050.026.1099221
TACATGA2950.025.4890212
TACATGG40500.024.9481642
ATTAACG1551.5477854E-624.255682
TTAACGG1551.5477854E-624.255683
GTACAAT1652.6587386E-622.7923931
CTATACA1252.4247689E-422.5577834
ATAATAC1050.002336637622.3787543
TATTAGG1903.6423444E-722.2609712
ACATGGG44500.021.9663813
CTTACTA1503.258379E-521.9615489
CCTAGAC1303.161387E-421.6901763
CCGTAAG1100.00306103921.3615382
TGATCGT1100.003063670821.3583726
TATACAG1354.0792933E-420.8868375
CATGGGG31850.020.8048634
TAGGACT3650.020.6007164
CGCGCTT805.341744E-520.5991412-13
Produced by FastQC (version 0.11.5)