##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1013500 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.516825851011347 33.0 31.0 34.0 30.0 34.0 2 31.524788357178096 34.0 31.0 34.0 30.0 34.0 3 31.667994079921066 34.0 31.0 34.0 30.0 34.0 4 34.9645505673409 37.0 35.0 37.0 33.0 37.0 5 35.076512086827826 37.0 35.0 37.0 33.0 37.0 6 35.09484657128762 37.0 35.0 37.0 33.0 37.0 7 35.080467686235814 37.0 36.0 37.0 33.0 37.0 8 35.01562802170696 37.0 36.0 37.0 33.0 37.0 9 36.621712876171685 39.0 38.0 39.0 33.0 39.0 10-11 36.65084755796744 39.0 38.0 39.0 33.0 39.0 12-13 36.60138233843118 39.0 38.0 39.0 33.0 39.0 14-15 38.009881105081405 41.0 39.0 41.0 33.0 41.0 16-17 38.02466748889985 41.0 39.0 41.0 33.0 41.0 18-19 38.037503700049335 41.0 39.0 41.0 33.0 41.0 20-21 38.07126541687222 41.0 39.0 41.0 33.0 41.0 22-23 38.052344844597926 40.5 39.0 41.0 33.0 41.0 24-25 38.055252590034534 40.5 38.0 41.0 33.0 41.0 26-27 37.932092747903305 40.0 38.0 41.0 32.0 41.0 28-29 37.8980439072521 40.0 38.0 41.0 32.0 41.0 30-31 37.99295855944746 40.5 38.0 41.0 33.0 41.0 32-33 37.931746916625556 41.0 38.0 41.0 32.5 41.0 34-35 37.83987617168229 41.0 38.0 41.0 32.0 41.0 36-37 37.72035816477553 40.0 38.0 41.0 32.0 41.0 38-39 37.59440207202763 40.0 38.0 41.0 32.0 41.0 40-41 37.485978786383825 40.0 38.0 41.0 31.0 41.0 42-43 37.32993438579181 40.0 38.0 41.0 31.0 41.0 44-45 37.167124321657624 40.0 37.0 41.0 31.0 41.0 46-47 36.98947804637395 40.0 37.0 41.0 30.5 41.0 48-49 36.82462062160829 40.0 36.5 41.0 30.0 41.0 50-51 36.63916625555007 40.0 36.0 41.0 30.0 41.0 52-53 36.438443512580164 40.0 35.5 41.0 30.0 41.0 54-55 36.206791317217565 39.0 35.0 41.0 30.0 41.0 56-57 35.95974346324618 39.0 35.0 41.0 30.0 41.0 58-59 35.67850518006907 39.0 35.0 41.0 30.0 41.0 60-61 35.374169215589546 38.0 35.0 40.0 29.0 41.0 62-63 35.03244647261964 37.0 35.0 40.0 29.0 41.0 64-65 34.68465268870251 37.0 34.5 40.0 29.0 41.0 66-67 34.32047360631475 36.0 34.0 39.0 28.0 41.0 68-69 33.950553527380364 35.5 34.0 39.0 28.0 41.0 70-71 33.5878505180069 35.0 34.0 38.5 27.0 40.0 72-73 33.23277701036014 35.0 34.0 37.0 27.0 39.5 74-75 32.90542821904292 35.0 33.5 37.0 26.0 39.0 76-77 32.59517414898865 35.0 33.0 36.0 26.0 39.0 78-79 32.282527380365075 35.0 33.0 36.0 26.0 37.5 80-81 32.01959644795264 35.0 33.0 35.0 25.0 37.0 82-83 31.902005426739024 35.0 33.0 35.0 25.0 37.0 84-85 31.800265416872225 35.0 33.0 35.0 25.0 36.0 86-87 31.741364084854467 35.0 33.0 35.0 25.0 36.0 88-89 31.599626541687222 35.0 33.0 35.0 25.0 36.0 90-91 31.45999062654169 35.0 33.0 35.0 25.0 35.5 92-93 31.29428268376912 35.0 33.0 35.0 24.0 35.0 94-95 31.137961026147018 35.0 33.0 35.0 24.0 35.0 96-97 31.000185495806612 35.0 33.0 35.0 23.5 35.0 98-99 30.8454494326591 35.0 33.0 35.0 21.5 35.0 100 30.718941292550568 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18769529792660222 1101 2 0.31943939548009226 1101 3 0.2898252996467683 1101 4 0.16529321292571098 1101 5 0.20190293425356032 1101 6 0.2684207754572796 1101 7 0.36699005503702864 1101 8 0.3758065353353004 1101 9 0.4451355786634039 1101 10-11 0.4657181681692606 1101 12-13 0.4530490502323161 1101 14-15 0.4801115566447436 1101 16-17 0.5250955953195984 1101 18-19 0.4386714063204664 1101 20-21 0.40676175008390913 1101 22-23 0.4580184742826816 1101 24-25 0.5501104090796716 1101 26-27 0.3050017735085504 1101 28-29 0.3237550165014724 1101 30-31 0.41275179365493386 1101 32-33 0.5154258013189192 1101 34-35 0.3924103321938972 1101 36-37 0.4930993103253911 1101 38-39 0.4933665313318727 1101 40-41 0.4707189850830815 1101 42-43 0.4439434470201249 1101 44-45 0.4968509474957017 1101 46-47 0.4659971253813495 1101 48-49 0.3749926141193711 1101 50-51 0.32687299449860063 1101 52-53 0.3743199872386427 1101 54-55 0.2600706509510573 1101 56-57 0.3806520937894149 1101 58-59 0.2847061627560947 1101 60-61 0.4056544883848687 1101 62-63 0.41222297580101497 1101 64-65 0.37841993543339214 1101 66-67 0.2725474106364203 1101 68-69 0.40699997994545356 1101 70-71 0.3959393110553364 1101 72-73 0.3507658829491547 1101 74-75 0.31054146922869563 1101 76-77 0.32239318452482735 1101 78-79 0.262688802286668 1101 80-81 0.41721937377945295 1101 82-83 0.4082360039944568 1101 84-85 0.3486337798396342 1101 86-87 0.17954581458899455 1101 88-89 0.24753367661010728 1101 90-91 0.34293992328361256 1101 92-93 0.2959553570734599 1101 94-95 0.4527484335541203 1101 96-97 0.4064653473697959 1101 98-99 0.32484566547317684 1101 100 0.3330407608903698 1102 1 0.07776674193681288 1102 2 0.10472707007394533 1102 3 0.08177543488215733 1102 4 -0.12293459006874485 1102 5 -0.06875069373519693 1102 6 -0.11267197323856237 1102 7 0.05502332936917753 1102 8 -0.047446357767398695 1102 9 -0.01291983571769606 1102 10-11 0.038952075741853776 1102 12-13 0.04547119495462937 1102 14-15 0.13221281669977714 1102 16-17 0.19231517903994444 1102 18-19 0.0504684305576788 1102 20-21 -0.02615886703344472 1102 22-23 0.052179109392476164 1102 24-25 0.021147836861636904 1102 26-27 -0.014060282887228936 1102 28-29 0.08964356851890898 1102 30-31 0.09626505139664943 1102 32-33 0.08905110289676088 1102 34-35 0.10576154113403646 1102 36-37 0.14618723377574128 1102 38-39 0.12461525345965896 1102 40-41 0.051824585522375344 1102 42-43 -0.008734973150104963 1102 44-45 0.06676998151193203 1102 46-47 0.009122610972795542 1102 48-49 0.014129970048408325 1102 50-51 0.010118118138215948 1102 52-53 -0.09708342314673502 1102 54-55 -0.01685112282320489 1102 56-57 0.026533986267054388 1102 58-59 -0.035631429177684026 1102 60-61 -0.0031664809870974864 1102 62-63 -0.07573055930468797 1102 64-65 -0.07487127923161552 1102 66-67 0.014989417144079198 1102 68-69 -0.007923230887392663 1102 70-71 -0.11887874914923913 1102 72-73 -0.07194343982238394 1102 74-75 -0.00702423530972851 1102 76-77 0.01776772522104153 1102 78-79 0.032909560189033016 1102 80-81 -0.0926297350689751 1102 82-83 -0.12762941488475477 1102 84-85 -0.13919182200167413 1102 86-87 -0.06511895936636236 1102 88-89 -0.08537759378473098 1102 90-91 0.02100316554481907 1102 92-93 -0.05234558402466405 1102 94-95 -0.010042556205377196 1102 96-97 0.022045457544287217 1102 98-99 0.015152237971612692 1102 100 -0.06411024125353748 1103 1 -0.08749511046266534 1103 2 -0.030231768666631353 1103 3 -0.029391822418006086 1103 4 0.0099425676842273 1103 5 0.00603767785199949 1103 6 0.014496215600111384 1103 7 0.039999524605590864 1103 8 0.02632973464825028 1103 9 0.09361005150901747 1103 10-11 0.06908619312009989 1103 12-13 0.039829509924494744 1103 14-15 0.11271037690374897 1103 16-17 0.10273588731679695 1103 18-19 0.14571934177371304 1103 20-21 0.008096868849023053 1103 22-23 -0.033914877130975185 1103 24-25 -0.05078852192130512 1103 26-27 -0.17490364380787327 1103 28-29 -0.11678558512123516 1103 30-31 -0.038063515857579944 1103 32-33 -0.13148020360796409 1103 34-35 -0.23441973538130867 1103 36-37 -0.25240716468985624 1103 38-39 -0.20941105810921812 1103 40-41 -0.1887754439177627 1103 42-43 -0.21170326349132296 1103 44-45 -0.14397385418575936 1103 46-47 -0.04282494165143902 1103 48-49 -0.13649008527267625 1103 50-51 -0.16689946465117345 1103 52-53 -0.18510777057544914 1103 54-55 -0.17044045714666112 1103 56-57 -0.03593264641895644 1103 58-59 0.006788450820820913 1103 60-61 -0.06024545963420991 1103 62-63 -0.05718794471336963 1103 64-65 -0.03667600327431586 1103 66-67 0.044552857333371776 1103 68-69 0.07895344571714702 1103 70-71 0.012785650920186242 1103 72-73 0.03622817417792845 1103 74-75 0.04781405268067829 1103 76-77 -0.03234668484484615 1103 78-79 -0.03672019719351738 1103 80-81 -0.08645884169278872 1103 82-83 -0.1242113935692224 1103 84-85 -0.02810475610631258 1103 86-87 -0.16467356751600803 1103 88-89 -0.11168559249287924 1103 90-91 -0.04910417010124846 1103 92-93 -0.039124987356672136 1103 94-95 -0.14934436369767212 1103 96-97 -0.020328559561747284 1103 98-99 -0.1043557493120062 1103 100 -0.1284199031094495 1104 1 -0.12012399940316243 1104 2 -0.185639499153055 1104 3 -0.009046992647885332 1104 4 -0.23331188729937935 1104 5 -0.07357938672494413 1104 6 -0.15715444528490963 1104 7 -0.25629831593333563 1104 8 -0.18179468611415928 1104 9 -0.10300614539903563 1104 10-11 -0.1402566057559369 1104 12-13 -0.09927063722032159 1104 14-15 -0.06793839030977011 1104 16-17 -0.17863583174425912 1104 18-19 -0.051616218083829324 1104 20-21 -0.15367614179311317 1104 22-23 -0.07660086121737919 1104 24-25 -0.20523595828696983 1104 26-27 -0.2383271253345498 1104 28-29 -0.18223547460428335 1104 30-31 -0.07821616013655586 1104 32-33 -0.10622966685659208 1104 34-35 -0.07575507990496533 1104 36-37 -0.06029054872517037 1104 38-39 -0.04999801183143404 1104 40-41 0.02400134984515745 1104 42-43 -0.014793621388903944 1104 44-45 0.02131838767218852 1104 46-47 -0.07865903322745993 1104 48-49 -0.05732611907587426 1104 50-51 -0.08571225717275155 1104 52-53 0.02179906374201579 1104 54-55 0.051961708436650156 1104 56-57 0.024980717581854606 1104 58-59 0.04361555882343282 1104 60-61 0.05427390247082542 1104 62-63 0.11675934704081925 1104 64-65 -0.038749119683394895 1104 66-67 0.01750444098742321 1104 68-69 0.030913670171052843 1104 70-71 0.028385746342657114 1104 72-73 -0.010832834977982486 1104 74-75 0.06624568311707435 1104 76-77 -0.0725352181044272 1104 78-79 -0.0029618609222126224 1104 80-81 0.03529188611462786 1104 82-83 -0.03072323798837928 1104 84-85 -0.0016872997061447847 1104 86-87 0.0016393991726850743 1104 88-89 -0.037451083338023494 1104 90-91 -0.10001400853750297 1104 92-93 -0.11152995016935563 1104 94-95 -0.15736080085078896 1104 96-97 -0.21181738225540414 1104 98-99 -0.10422354064080963 1104 100 -0.018403816401672657 1105 1 -0.24052464525795259 1105 2 -0.26177780322952415 1105 3 -0.21952998948607316 1105 4 -0.1304568633097034 1105 5 -0.14243558308798754 1105 6 -0.11868500719956643 1105 7 -0.2092469648804709 1105 8 -0.21286407952338493 1105 9 -0.06639121607875609 1105 10-11 -0.08411411391783474 1105 12-13 -0.08002267525599649 1105 14-15 -0.08164956168867121 1105 16-17 -0.16190827262461482 1105 18-19 -0.09636879782995322 1105 20-21 -0.10113222651582987 1105 22-23 -0.12145413714139153 1105 24-25 -0.17052451155524295 1105 26-27 -0.19190868599037714 1105 28-29 -0.1579821879859864 1105 30-31 -0.21617192278142028 1105 32-33 -0.30592492973858043 1105 34-35 -0.285035219670263 1105 36-37 -0.435015508234315 1105 38-39 -0.2956387168745849 1105 40-41 -0.3219672299489389 1105 42-43 -0.3337966142892057 1105 44-45 -0.24387561613278308 1105 46-47 -0.206308554558305 1105 48-49 -0.21006123215793338 1105 50-51 -0.27887676074559664 1105 52-53 -0.17932968294287832 1105 54-55 -0.21142051376558157 1105 56-57 -0.10353908858174776 1105 58-59 -0.20772085186505507 1105 60-61 -0.16593939352246423 1105 62-63 -0.2148036825198787 1105 64-65 -0.1408435502102776 1105 66-67 -0.16005165527911913 1105 68-69 -0.15204154026896788 1105 70-71 -0.3036113012354136 1105 72-73 -0.32304845572868857 1105 74-75 -0.28130455128264487 1105 76-77 -0.13832230490653075 1105 78-79 -0.28491740568354373 1105 80-81 -0.27025554878141733 1105 82-83 -0.337289378386334 1105 84-85 -0.23881305598941438 1105 86-87 -0.19559742774332634 1105 88-89 -0.18389056143697857 1105 90-91 -0.21911409953886363 1105 92-93 -0.0985396434882766 1105 94-95 -0.15650975573440462 1105 96-97 -0.22344541707079557 1105 98-99 -0.21484891138014817 1105 100 -0.20772512416267475 1106 1 0.2172548683465898 1106 2 0.2213545801586143 1106 3 0.2395807912580068 1106 4 0.30593978430486857 1106 5 0.33983868593995936 1106 6 0.3515630620431267 1106 7 0.38204422425884843 1106 8 0.43674808918218133 1106 9 0.3636584649630734 1106 10-11 0.4233828378863791 1106 12-13 0.45734799515110325 1106 14-15 0.4321509610191612 1106 16-17 0.5248337905174907 1106 18-19 0.5594986465990885 1106 20-21 0.41424873846386134 1106 22-23 0.42596193073503485 1106 24-25 0.5351002670918419 1106 26-27 0.4452678237939409 1106 28-29 0.5158615624077285 1106 30-31 0.5850500152691609 1106 32-33 0.540121934784338 1106 34-35 0.6310599540314072 1106 36-37 0.5905795373801368 1106 38-39 0.5605151389706009 1106 40-41 0.6259558673259491 1106 42-43 0.6384392565708836 1106 44-45 0.47523121700527327 1106 46-47 0.3927098634035815 1106 48-49 0.5373752736787836 1106 50-51 0.559589160355479 1106 52-53 0.5601031216632251 1106 54-55 0.5038290043325091 1106 56-57 0.5884370521994029 1106 58-59 0.6237842639243922 1106 60-61 0.4818011619700968 1106 62-63 0.5730929853926625 1106 64-65 0.5311747823601323 1106 66-67 0.5568461983150854 1106 68-69 0.48597068611083927 1106 70-71 0.5311673878514185 1106 72-73 0.45133116118554284 1106 74-75 0.5217519036831462 1106 76-77 0.443046674469322 1106 78-79 0.36440429201103086 1106 80-81 0.40450650996512394 1106 82-83 0.4258948558270852 1106 84-85 0.4506818324207309 1106 86-87 0.4531664256241079 1106 88-89 0.4723359471574575 1106 90-91 0.5068800462453886 1106 92-93 0.536897539880826 1106 94-95 0.508001826009739 1106 96-97 0.5950850935842382 1106 98-99 0.6069383742487489 1106 100 0.6165840097857576 1107 1 -0.21546234872233683 1107 2 -0.16529446148051363 1107 3 -0.07796598723642134 1107 4 -0.1499862513554291 1107 5 -0.27149147184961464 1107 6 -0.1371516024356083 1107 7 0.023078631047525278 1107 8 -0.05005972964469407 1107 9 -0.17952665100504817 1107 10-11 -0.08321895044615246 1107 12-13 -0.082460256704465 1107 14-15 -0.11770811473046905 1107 16-17 -0.1353108562602472 1107 18-19 -0.03448757245265455 1107 20-21 -0.11267880899836058 1107 22-23 -0.1320905037219049 1107 24-25 -0.1351065990719036 1107 26-27 -0.1283356586683908 1107 28-29 -0.08850974830175318 1107 30-31 0.027217893656469982 1107 32-33 -0.143060598205615 1107 34-35 -0.04187803170143667 1107 36-37 0.13726306937487465 1107 38-39 0.08878735448215025 1107 40-41 0.05746896074619201 1107 42-43 0.026854599461238138 1107 44-45 0.003112187293424995 1107 46-47 0.07859312798411366 1107 48-49 0.11032541460303236 1107 50-51 0.15368173733202894 1107 52-53 0.14386378594442562 1107 54-55 -0.005545687039578695 1107 56-57 0.026460770016633717 1107 58-59 0.0011345841377092825 1107 60-61 0.10930911982197244 1107 62-63 0.11837867037568373 1107 64-65 0.02433748719410289 1107 66-67 0.18706628324949293 1107 68-69 0.05865873072038852 1107 70-71 0.08792453472411665 1107 72-73 0.1542962681892206 1107 74-75 0.08402067015913417 1107 76-77 0.10402319215892675 1107 78-79 0.05242990907692047 1107 80-81 0.014108351476615155 1107 82-83 0.022965454698177012 1107 84-85 -0.03456480400990358 1107 86-87 0.07345723857850217 1107 88-89 0.10176214615313839 1107 90-91 0.12573502721491892 1107 92-93 0.10934627915956341 1107 94-95 0.029478529825709643 1107 96-97 0.05139097897598077 1107 98-99 0.10539998283638852 1107 100 0.24298660491912116 1108 1 -0.06118381374320592 1108 2 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-0.29808419300514544 2214 70-71 -0.28498923170705126 2214 72-73 -0.21111971787885153 2214 74-75 -0.3052443914054024 2214 76-77 -0.29063725292104436 2214 78-79 -0.2959280998952778 2214 80-81 -0.23436797809939236 2214 82-83 -0.23583070069261325 2214 84-85 -0.292537037387838 2214 86-87 -0.2927383668105783 2214 88-89 -0.276156203625618 2214 90-91 -0.2981749194597718 2214 92-93 -0.2668543508031007 2214 94-95 -0.24736034704702803 2214 96-97 -0.3047686259736757 2214 98-99 -0.28198005321807784 2214 100 -0.2527543936203287 2215 1 -0.33677844603240104 2215 2 -0.4063220134913976 2215 3 -0.5049291682589114 2215 4 -0.5141495557162372 2215 5 -0.44431272530986377 2215 6 -0.5090256258920078 2215 7 -0.5856485230175252 2215 8 -0.553790299019731 2215 9 -0.6127801555002605 2215 10-11 -0.6163972351585585 2215 12-13 -0.6572615948975837 2215 14-15 -0.744275423991624 2215 16-17 -0.725172397933946 2215 18-19 -0.8376616675337729 2215 20-21 -0.7374636237281607 2215 22-23 -0.7036118653105845 2215 24-25 -0.6485994932913144 2215 26-27 -0.6067915513917086 2215 28-29 -0.6236777621952925 2215 30-31 -0.6432141983660813 2215 32-33 -0.5717210638492531 2215 34-35 -0.6567767684515573 2215 36-37 -0.5375626046303239 2215 38-39 -0.644575799439103 2215 40-41 -0.6141493292758469 2215 42-43 -0.7007550707055472 2215 44-45 -0.5110684355927404 2215 46-47 -0.5798086382980614 2215 48-49 -0.5514868221622962 2215 50-51 -0.4372069899326547 2215 52-53 -0.4843754005204346 2215 54-55 -0.5107943063970666 2215 56-57 -0.6181599784755178 2215 58-59 -0.47752422224601787 2215 60-61 -0.598124986818668 2215 62-63 -0.7400997675417216 2215 64-65 -0.793620786567196 2215 66-67 -0.7437482820105856 2215 68-69 -0.8049774129383351 2215 70-71 -0.7504863644011763 2215 72-73 -0.7788739276311105 2215 74-75 -0.8142440550812537 2215 76-77 -0.7541154431551043 2215 78-79 -0.8420466912101361 2215 80-81 -0.9278472799132622 2215 82-83 -0.8386838980521851 2215 84-85 -0.8680888370070505 2215 86-87 -0.8497193730468275 2215 88-89 -0.8846607867546865 2215 90-91 -0.8134290722079385 2215 92-93 -0.8092480096678081 2215 94-95 -0.8659981202391407 2215 96-97 -0.8884058861289539 2215 98-99 -0.7806346141155487 2215 100 -0.8047102040475203 2216 1 -0.12282463783423125 2216 2 -0.1499250073736107 2216 3 -0.08975096892236678 2216 4 -0.22579669102437805 2216 5 -0.16189844935927056 2216 6 -0.2868828163769308 2216 7 -0.08893662583637507 2216 8 -0.09601470682783031 2216 9 -1.3450379744739394E-4 2216 10-11 -0.143598055308928 2216 12-13 -0.19616456044126807 2216 14-15 -0.17279773641557483 2216 16-17 -0.3010258877404084 2216 18-19 -0.2749855935868837 2216 20-21 -0.20365742592522906 2216 22-23 -0.2476866123475432 2216 24-25 -0.3852647569229788 2216 26-27 -0.3989440710203098 2216 28-29 -0.24525421787883772 2216 30-31 -0.5151667646699067 2216 32-33 -0.5122653719724113 2216 34-35 -0.5973731694584146 2216 36-37 -0.6521638231870668 2216 38-39 -0.524454818926003 2216 40-41 -0.47013542672704745 2216 42-43 -0.5046764146185936 2216 44-45 -0.4690169761585139 2216 46-47 -0.4905704029549227 2216 48-49 -0.565857165058496 2216 50-51 -0.46539861673090144 2216 52-53 -0.5664112737638618 2216 54-55 -0.5695727907858696 2216 56-57 -0.6703570479382535 2216 58-59 -0.540199254335235 2216 60-61 -0.48711088423318927 2216 62-63 -0.5953857366360538 2216 64-65 -0.5773384264678398 2216 66-67 -0.5009350162451938 2216 68-69 -0.5552930958135249 2216 70-71 -0.5444402559479684 2216 72-73 -0.4953365291080516 2216 74-75 -0.607437390526087 2216 76-77 -0.45550028037525436 2216 78-79 -0.4298982139893113 2216 80-81 -0.38390172322786853 2216 82-83 -0.5815961402965932 2216 84-85 -0.43409885551044525 2216 86-87 -0.4372864686810267 2216 88-89 -0.6162032362037522 2216 90-91 -0.558479206399209 2216 92-93 -0.9240263440291088 2216 94-95 -0.6410422950045174 2216 96-97 -0.5731282935703206 2216 98-99 -0.5598326754267902 2216 100 -0.4888731339179948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11677.0 3 2116.0 4 1290.0 5 1333.0 6 2211.0 7 3924.0 8 4331.0 9 3362.0 10 2460.0 11 2536.0 12 2578.0 13 2362.0 14 2593.0 15 2581.0 16 2394.0 17 2449.0 18 2178.0 19 1957.0 20 1776.0 21 1896.0 22 2405.0 23 3944.0 24 6333.0 25 7472.0 26 6691.0 27 5911.0 28 6972.0 29 8641.0 30 10938.0 31 14271.0 32 18835.0 33 26272.0 34 39272.0 35 63619.0 36 127222.0 37 259823.0 38 284353.0 39 62448.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.295585605953654 17.945465010549015 12.970694773672587 24.78825460982475 2 17.2894087454348 20.494719178758267 35.80229000098707 26.413582074819857 3 19.30935422250522 23.52154319250912 28.502959134817097 28.666143450168562 4 14.343081873718239 16.88720110351147 34.042578401547445 34.72713862122285 5 15.350401624272445 36.961777061496804 32.32971703609779 15.358104278132956 6 33.01785432137779 35.1030968557434 16.452144888607993 15.426903934270817 7 30.03560019157939 31.837927782468512 19.66937741634449 18.45709460960761 8 26.344513995745277 34.354611554119316 19.051734184541175 20.249140265594228 9 26.00194557332688 17.12924236214329 18.822582477001248 38.04622958752858 10-11 25.4322803215973 26.370247755944764 26.094398219866793 22.10307370259114 12-13 25.667223056257903 24.43737160240202 25.74549620733249 24.149909134007586 14-15 23.641724117496 25.900776326966003 22.958190940876676 27.499308614661324 16-17 23.832949307049383 28.18779255569945 24.13253424268651 23.84672389456467 18-19 23.761413571898036 27.28013427412127 25.524526803715947 23.43392535026475 20-21 24.851658750689428 26.552454068157232 24.765495655059254 23.830391526094086 22-23 24.79349938940778 26.304381949373663 24.594328847101192 24.307789814117367 24-25 24.72565565571987 26.172689766249096 24.655290075443155 24.446364502587883 26-27 24.33665536917495 26.26992999113399 24.99532977704504 24.398084862646023 28-29 24.284128954037936 26.37489793722115 25.068445002601553 24.272528106139365 30-31 24.553055456127577 26.522371295622015 24.526148187462198 24.39842506078821 32-33 24.21221056955242 26.560978054111107 24.786220985370562 24.440590390965912 34-35 24.580465836011474 26.290577338419702 24.69854578595702 24.4304110396118 36-37 24.56073587291622 26.355798266734187 24.788326961177727 24.295138899171867 38-39 24.285028752883186 26.728135170147553 24.764111820278682 24.222724256690576 40-41 24.24023390634859 26.361614791159983 24.980966966949175 24.417184335542252 42-43 24.18018441438288 26.417654095412253 24.776566527461473 24.625594962743396 44-45 24.086273441133173 26.442457220209654 24.743607591999627 24.727661746657542 46-47 24.62094992836188 26.351027767546896 24.69653777071655 24.331484533374674 48-49 24.153424760332438 26.48932567239723 24.59094333586438 24.766306231405956 50-51 23.861261106487007 26.630539420562023 24.81904287975324 24.68915659319773 52-53 24.159507251900482 26.361490132498133 24.70079554172768 24.778207073873705 54-55 23.96178372876202 26.060874724914523 24.88263513373424 25.09470641258921 56-57 23.945706852547552 26.14769434310623 24.821910775200305 25.084688029145912 58-59 24.312486147044467 25.71251704931677 24.959656737954163 25.015340065684605 60-61 24.06993665637267 25.99176809281974 24.77598707853188 25.16230817227571 62-63 24.10125677432859 25.864186621460046 24.911411676775298 25.12314492743607 64-65 24.336249329251828 26.04650290291417 24.728060055594966 24.88918771223904 66-67 24.214585185561823 26.06741428820371 24.44897179412878 25.26902873210568 68-69 24.136261136737012 25.949189624668268 24.855558100593957 25.058991138000756 70-71 24.338665865034574 25.70272382145521 25.20278971916715 24.755820594343064 72-73 24.353018817725772 25.6584081516001 25.06731546184947 24.92125756882466 74-75 24.371940531610168 25.958557330309795 24.7650530657496 24.904449072330436 76-77 24.49705275232754 25.375698805207215 25.100569705788573 25.026678736676672 78-79 24.457655896782853 25.609618858648975 24.97724602916062 24.955479215407546 80-81 24.638386438757294 25.564784438487813 25.12695403595135 24.669875086803543 82-83 24.411700249949657 25.480304125156465 25.22966345641281 24.87833216848107 84-85 24.43902212720006 25.631257421549957 24.869977899450895 25.059742551799086 86-87 24.560169693954702 25.37532646740064 25.399904058559812 24.66459978008485 88-89 24.254471100529617 25.46537102595575 25.096795495363438 25.18336237815119 90-91 24.47087982130457 25.165762611653797 25.20529394084909 25.15806362619254 92-93 24.553639509214445 25.23811568432071 25.37502078993358 24.833224016531265 94-95 24.136811241725425 25.07919853403131 25.50540731414933 25.278582910093935 96-97 24.405272776716394 25.052747015197557 25.231901789622942 25.31007841846311 98-99 23.966792454320128 25.600765189031893 25.31732345720933 25.11511889943864 100 23.962851597898556 25.483752509827074 25.36924319212492 25.184152700149454 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 948.0 1 657.5 2 356.5 3 327.0 4 269.5 5 234.0 6 216.0 7 203.0 8 210.5 9 193.5 10 188.0 11 191.5 12 185.0 13 176.0 14 171.0 15 184.5 16 203.5 17 244.0 18 293.5 19 352.5 20 456.0 21 571.0 22 727.5 23 926.5 24 1271.0 25 1825.0 26 2369.5 27 2954.0 28 3648.0 29 4404.5 30 5148.0 31 6030.5 32 7114.0 33 8720.0 34 10632.0 35 12228.0 36 14412.0 37 17010.0 38 19299.5 39 21756.5 40 25145.0 41 28005.0 42 28174.5 43 29059.5 44 31705.0 45 32720.5 46 35525.5 47 38147.5 48 39420.5 49 40757.5 50 41059.0 51 42046.0 52 42231.0 53 44014.0 54 44301.0 55 42133.0 56 39746.0 57 35027.0 58 30499.0 59 28644.0 60 27127.0 61 22841.0 62 19139.0 63 16725.5 64 13723.5 65 11727.5 66 9173.0 67 6469.5 68 5286.0 69 4401.0 70 3369.5 71 2387.0 72 1770.0 73 1299.0 74 995.5 75 679.5 76 410.0 77 302.5 78 198.5 79 110.5 80 63.5 81 30.5 82 18.0 83 14.0 84 10.5 85 7.0 86 4.5 87 3.5 88 4.0 89 3.0 90 3.0 91 2.5 92 0.5 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06008880118401579 2 0.03946719289590528 3 0.05278737049827331 4 0.07232363098174642 5 0.08505180069067587 6 0.08485446472619634 7 0.08534780463739516 8 0.09649728663048841 9 0.09324124321657623 10-11 0.09782930439072521 12-13 0.10064134188455846 14-15 0.10261470152935373 16-17 0.07553034040453872 18-19 0.09319190922545634 20-21 0.08737049827331031 22-23 0.05416872224962999 24-25 0.021756290083867785 26-27 0.17474099654662062 28-29 0.06344351258016774 30-31 0.07523433645781943 32-33 0.07039960532807103 34-35 0.020078934385791808 36-37 0.04923532313764183 38-39 0.07222496299950666 40-41 0.07765170202269363 42-43 0.07168228909718796 44-45 0.06877158362111495 46-47 0.07607301430685742 48-49 0.07331031080414405 50-51 0.06817957572767637 52-53 0.07212629501726689 54-55 0.0624568327577701 56-57 0.06561420818944252 58-59 0.06211149481993093 60-61 0.060384805130735075 62-63 0.06600888011840159 64-65 0.0629995066600888 66-67 0.06349284657128762 68-69 0.06275283670448939 70-71 0.05589541193882585 72-73 0.053823384311790824 74-75 0.05510606808090775 76-77 0.051406018746916624 78-79 0.048347311297483965 80-81 0.04306857424765664 82-83 0.048939319190922544 84-85 0.03976319684262457 86-87 0.03769116921558954 88-89 0.04050320670942279 90-91 0.03744449925999013 92-93 0.038431179082387766 94-95 0.03764183522446966 96-97 0.040404538727183026 98-99 0.04114454859398125 100 0.04755796743956586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1013500.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.03970398398885 #Duplication Level Percentage of deduplicated Percentage of total 1 62.211998050679554 16.199820134921865 2 10.531010588493011 5.484487967532204 3 5.530605095113128 4.320459585872589 4 3.7214546190876927 3.876223066835659 5 2.7599044876396204 3.593354794110906 6 2.111684333616403 3.299258097299872 7 1.685156461325104 3.07166827937283 8 1.3837105820480164 2.8825131164834605 9 1.1331163171690908 2.6555412130659666 >10 8.321259221527612 41.478802337478974 >50 0.4992074445930566 8.688183806297173 >100 0.10975737756049728 4.130768684318296 >500 3.784737157242537E-4 0.07046570619073636 >1k 7.569474314485073E-4 0.24845321021936556 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1417 0.13981253083374445 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1104 0.10892945239269858 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 9.86679822397632E-5 0.0 0.0 0.0 14-15 0.0 9.86679822397632E-5 0.0 0.0 0.0 16-17 0.0 9.86679822397632E-5 0.0 0.0 0.0 18-19 0.0 9.86679822397632E-5 0.0 9.86679822397632E-5 0.0 20-21 0.0 9.86679822397632E-5 0.0 2.960039467192896E-4 0.0 22-23 0.0 9.86679822397632E-5 0.0 7.400098667982239E-4 0.0 24-25 0.0 9.86679822397632E-5 0.0 0.0022693635915145534 0.0 26-27 0.0 9.86679822397632E-5 0.0 0.00493339911198816 0.0 28-29 0.0 9.86679822397632E-5 0.0 0.012234829797730636 0.0 30-31 0.0 9.86679822397632E-5 0.0 0.03295510606808091 0.0 32-33 0.0 9.86679822397632E-5 0.0 0.07972372964972865 0.0 34-35 0.0 9.86679822397632E-5 0.0 0.16122348297977307 0.0 36-37 0.0 9.86679822397632E-5 0.0 0.2750863344844598 0.0 38-39 0.0 9.86679822397632E-5 0.0 0.4199309324124322 0.0 40-41 0.0 9.86679822397632E-5 0.0 0.5717316230883078 0.0 42-43 0.0 9.86679822397632E-5 0.0 0.7171188949185989 0.0 44-45 0.0 9.86679822397632E-5 0.0 0.9034533793783917 0.0 46-47 0.0 9.86679822397632E-5 0.0 1.1215096201282684 0.0 48-49 0.0 9.86679822397632E-5 0.0 1.3359151455352738 0.0 50-51 0.0 9.86679822397632E-5 0.0 1.5466699555994081 0.0 52-53 0.0 9.86679822397632E-5 0.0 1.7652195362604834 0.0 54-55 0.0 9.86679822397632E-5 0.0 2.014306857424766 0.0 56-57 0.0 9.86679822397632E-5 0.0 2.3059200789343857 0.0 58-59 0.0 9.86679822397632E-5 0.0 2.603108041440553 0.0 60-61 0.0 9.86679822397632E-5 0.0 2.905772076961026 0.0 62-63 0.0 9.86679822397632E-5 0.0 3.2185495806610755 0.0 64-65 0.0 9.86679822397632E-5 0.0 3.564331524420326 0.0 66-67 0.0 9.86679822397632E-5 0.0 3.960483473112975 0.0 68-69 0.0 9.86679822397632E-5 0.0 4.399013320177603 0.0 70-71 0.0 9.86679822397632E-5 0.0 4.853971386285151 0.0 72-73 0.0 9.86679822397632E-5 0.0 5.3256536753823385 0.0 74-75 0.0 9.86679822397632E-5 0.0 5.8368031573754315 0.0 76-77 0.0 9.86679822397632E-5 0.0 6.399309324124322 0.0 78-79 0.0 9.86679822397632E-5 0.0 7.005229403058707 0.0 80-81 0.0 9.86679822397632E-5 0.0 7.652343364578194 0.0 82-83 0.0 9.86679822397632E-5 0.0 8.30009866798224 0.0 84-85 0.0 9.86679822397632E-5 0.0 8.981943759250123 0.0 86-87 0.0 9.86679822397632E-5 0.0 9.704834731129747 0.0 88 0.0 9.86679822397632E-5 0.0 10.288209176122349 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACTAC 70 0.008846157 26.850525 6 CCTCGCG 160 6.828668E-8 26.471512 9 GTACATG 4105 0.0 26.109922 1 TACATGA 295 0.0 25.489021 2 TACATGG 4050 0.0 24.948164 2 ATTAACG 155 1.5477854E-6 24.25568 2 TTAACGG 155 1.5477854E-6 24.25568 3 GTACAAT 165 2.6587386E-6 22.792393 1 CTATACA 125 2.4247689E-4 22.557783 4 ATAATAC 105 0.0023366376 22.378754 3 TATTAGG 190 3.6423444E-7 22.260971 2 ACATGGG 4450 0.0 21.966381 3 CTTACTA 150 3.258379E-5 21.961548 9 CCTAGAC 130 3.161387E-4 21.690176 3 CCGTAAG 110 0.003061039 21.361538 2 TGATCGT 110 0.0030636708 21.358372 6 TATACAG 135 4.0792933E-4 20.886837 5 CATGGGG 3185 0.0 20.804863 4 TAGGACT 365 0.0 20.600716 4 CGCGCTT 80 5.341744E-5 20.59914 12-13 >>END_MODULE