FastQCFastQC Report
Wed 25 May 2016
SRR1033095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013500
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.33093241243216576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.33053774050320667No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16640.16418352244696596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.15520473606314752No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11670.11514553527380365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.038.6178471
GTATCAA55650.028.8913691
CCGTTAA1554.9278242E-827.3772431
CGTTAAT1554.9538357E-827.3623492
GTCGTAA909.341863E-426.1942771
TCAACGC62850.024.976134
GTATATA950.001280524324.815631
TATCAAC66100.024.3105872
ATCAACG64900.024.1872083
AACGCAG65850.023.8370886
GTACATG37250.023.7963581
CAACGCA66250.023.7641055
CGTAAAC1000.001758864123.4992473
CGACCGT701.9331073E-523.49866718-19
TACATGG37850.023.1573892
ATTAACG2052.903107E-822.9873372
CTATACA1503.2916905E-521.932634
ACATGGG40200.021.1610153
CCGGTGC1353.990007E-420.9554211
ACGCAGA75000.020.9289637
Produced by FastQC (version 0.11.5)