##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033095_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1013500 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.637823384311794 34.0 31.0 34.0 31.0 34.0 2 32.630616674889 34.0 31.0 34.0 31.0 34.0 3 32.714015786877155 34.0 31.0 34.0 31.0 34.0 4 36.2384272323631 37.0 35.0 37.0 35.0 37.0 5 36.21791909225456 37.0 37.0 37.0 35.0 37.0 6 36.28355204736063 37.0 37.0 37.0 35.0 37.0 7 36.234299950666006 37.0 37.0 37.0 35.0 37.0 8 36.24413616181549 37.0 37.0 37.0 35.0 37.0 9 38.04225061667489 39.0 39.0 39.0 35.0 39.0 10-11 38.00015293537247 39.0 38.5 39.0 35.0 39.0 12-13 37.968307350764675 39.0 38.0 39.0 35.0 39.0 14-15 39.415837691169216 41.0 39.0 41.0 36.0 41.0 16-17 39.40498125308338 41.0 39.0 41.0 36.0 41.0 18-19 39.42998273310311 41.0 39.0 41.0 36.0 41.0 20-21 39.42727972372965 41.0 39.0 41.0 36.0 41.0 22-23 39.343488899852 41.0 39.0 41.0 36.0 41.0 24-25 39.258431179082386 41.0 39.0 41.0 36.0 41.0 26-27 39.11310754810064 41.0 39.0 41.0 35.5 41.0 28-29 39.03775086334484 40.5 39.0 41.0 35.0 41.0 30-31 38.95997582634435 40.5 39.0 41.0 35.0 41.0 32-33 38.849878145041934 41.0 38.0 41.0 35.0 41.0 34-35 38.70854859398125 41.0 38.0 41.0 35.0 41.0 36-37 38.560628515046865 41.0 38.0 41.0 35.0 41.0 38-39 38.410394671928955 40.0 38.0 41.0 34.5 41.0 40-41 38.25110162802171 40.0 38.0 41.0 34.0 41.0 42-43 38.05780759743463 40.0 38.0 41.0 34.0 41.0 44-45 37.77848347311297 40.0 37.5 41.0 33.0 41.0 46-47 37.39646472619635 40.0 37.0 41.0 33.0 41.0 48-49 37.02898766650222 40.0 37.0 41.0 32.0 41.0 50-51 36.77894869264924 40.0 36.0 41.0 31.0 41.0 52-53 36.58852590034534 40.0 36.0 41.0 31.0 41.0 54-55 36.36521756290084 39.5 35.0 41.0 31.0 41.0 56-57 36.14164676862358 39.0 35.0 41.0 31.0 41.0 58-59 35.870878638381846 39.0 35.0 41.0 30.0 41.0 60-61 35.58994474592994 38.0 35.0 41.0 30.0 41.0 62-63 35.26990083867785 37.0 35.0 40.0 30.0 41.0 64-65 34.92969215589541 37.0 35.0 40.0 29.0 41.0 66-67 34.564243709916134 36.0 35.0 39.0 29.0 41.0 68-69 34.18909916132215 36.0 34.5 39.0 29.0 41.0 70-71 33.80781154415392 35.0 34.0 38.5 28.0 40.5 72-73 33.45222150962013 35.0 34.0 37.0 28.0 39.5 74-75 33.14363394178589 35.0 34.0 37.0 27.0 39.0 76-77 32.84285199802664 35.0 34.0 36.0 27.0 39.0 78-79 32.56221361618155 35.0 34.0 36.0 26.5 38.0 80-81 32.32741045880611 35.0 34.0 35.5 26.0 37.0 82-83 32.128695115934875 35.0 34.0 35.0 26.0 37.0 84-85 32.0193788850518 35.0 34.0 35.0 26.0 36.0 86-87 31.913373951652687 35.0 34.0 35.0 26.0 36.0 88-89 31.783165268870253 35.0 34.0 35.0 25.5 36.0 90-91 31.66951406018747 35.0 33.5 35.0 25.0 35.5 92-93 31.5423655648742 35.0 33.0 35.0 25.0 35.0 94-95 31.428458806117415 35.0 33.0 35.0 25.0 35.0 96-97 31.311579181055748 35.0 33.0 35.0 24.0 35.0 98-99 31.1993083374445 35.0 33.0 35.0 24.0 35.0 100 31.0979338924519 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18229613310404602 1101 2 0.1527340023669339 1101 3 0.20693543142423465 1101 4 0.1136341194151953 1101 5 0.13225325221809925 1101 6 0.10912774471538711 1101 7 0.13011272197497448 1101 8 0.13032609284417873 1101 9 0.20551984892791353 1101 10-11 0.22499051390329328 1101 12-13 0.2332710423312676 1101 14-15 0.33170681988332973 1101 16-17 0.37146939569090165 1101 18-19 0.2190100850600203 1101 20-21 0.24341740011451662 1101 22-23 0.2526760559365968 1101 24-25 0.31229123732290276 1101 26-27 0.30517021030865976 1101 28-29 0.22647020212748714 1101 30-31 0.2782920838837697 1101 32-33 0.29528876552411987 1101 34-35 0.39649849264876025 1101 36-37 0.3519210365737919 1101 38-39 0.3336362623648057 1101 40-41 0.4236985849700119 1101 42-43 0.38204511095410254 1101 44-45 0.4493586976877495 1101 46-47 0.5578880524801235 1101 48-49 0.6362624945952646 1101 50-51 0.479716804050895 1101 52-53 0.49056236540891973 1101 54-55 0.4113252658227111 1101 56-57 0.45151889661187283 1101 58-59 0.48376054244314304 1101 60-61 0.5846956029328823 1101 62-63 0.5280688940300422 1101 64-65 0.5346035499926955 1101 66-67 0.45334995557821856 1101 68-69 0.463342546739959 1101 70-71 0.4587103270588173 1101 72-73 0.41428918970079565 1101 74-75 0.4875704863317125 1101 76-77 0.5346092488397503 1101 78-79 0.435763753190507 1101 80-81 0.5907105652709816 1101 82-83 0.4986054247097229 1101 84-85 0.47567698272237635 1101 86-87 0.48134172897994176 1101 88-89 0.5202510178580084 1101 90-91 0.673248583033363 1101 92-93 0.6246628761467328 1101 94-95 0.6504500916852827 1101 96-97 0.7556651739821376 1101 98-99 0.6428240061213906 1101 100 0.4513912358796759 1102 1 0.2445365424904935 1102 2 0.22837177013592935 1102 3 0.24539544205612884 1102 4 0.16733512944539797 1102 5 0.21896046029367255 1102 6 0.18065237040836024 1102 7 0.1547886535803329 1102 8 0.17782263163599765 1102 9 0.2509618612772755 1102 10-11 0.24905569560942808 1102 12-13 0.24343969225562034 1102 14-15 0.29686954048058567 1102 16-17 0.31982495669871014 1102 18-19 0.3498314867178891 1102 20-21 0.34201568382258785 1102 22-23 0.3205686036778985 1102 24-25 0.3845691504334283 1102 26-27 0.29769283229799726 1102 28-29 0.27087618870326224 1102 30-31 0.17647414635491998 1102 32-33 0.19166194510387413 1102 34-35 0.28210045078711943 1102 36-37 0.1451341476887933 1102 38-39 0.07259836724671942 1102 40-41 0.15841523323533124 1102 42-43 0.24065461072920158 1102 44-45 0.25613069023373924 1102 46-47 0.06983579261788009 1102 48-49 0.04234282967526326 1102 50-51 0.09247303568604082 1102 52-53 0.11923840216490333 1102 54-55 0.12861144494097232 1102 56-57 0.045721884058053774 1102 58-59 0.10935917081161506 1102 60-61 -0.10431703266762327 1102 62-63 -0.08461840552656952 1102 64-65 -0.015887737109409272 1102 66-67 0.1330389433865875 1102 68-69 0.05671046294658311 1102 70-71 0.04395944642229921 1102 72-73 0.10754786608805489 1102 74-75 0.16132576576950441 1102 76-77 0.10887655342023095 1102 78-79 0.14140946567363955 1102 80-81 0.045282573005387405 1102 82-83 0.0064002992007630155 1102 84-85 0.03637605914935449 1102 86-87 0.08024728619064803 1102 88-89 0.09686055268331017 1102 90-91 0.0021191484516585035 1102 92-93 0.01218032823017623 1102 94-95 0.07509477018723132 1102 96-97 0.13820812509293745 1102 98-99 0.13489710917484743 1102 100 0.1587839092175649 1103 1 0.08783210164428823 1103 2 0.06624452285419835 1103 3 0.10672637770307603 1103 4 0.10237459899573764 1103 5 0.1775207279791715 1103 6 0.13869359098745093 1103 7 0.1559201213466821 1103 8 0.16299271431003604 1103 9 0.2509206670624806 1103 10-11 0.20261498561889368 1103 12-13 0.18774648699418606 1103 14-15 0.23375780639089783 1103 16-17 0.27805402289726544 1103 18-19 0.27484098885010155 1103 20-21 0.25683977903285893 1103 22-23 0.1921934243457457 1103 24-25 0.20802145632080027 1103 26-27 0.2224414377365136 1103 28-29 0.19538504381166888 1103 30-31 0.18061032014836798 1103 32-33 0.18119119958745245 1103 34-35 0.24652787317172908 1103 36-37 0.26310202059530496 1103 38-39 0.2745672859370316 1103 40-41 0.22705248466942862 1103 42-43 0.24096548973684406 1103 44-45 0.165330401984086 1103 46-47 0.1596152882249271 1103 48-49 0.11255919267649261 1103 50-51 0.08962651544375433 1103 52-53 0.17057682966638055 1103 54-55 0.05312414484275507 1103 56-57 0.03302555243034533 1103 58-59 0.08836989448960964 1103 60-61 0.22205237288338253 1103 62-63 0.06714272954431522 1103 64-65 0.09939042819912913 1103 66-67 0.11504283871153831 1103 68-69 0.032697531258676804 1103 70-71 0.12939817030243006 1103 72-73 0.12751607513935426 1103 74-75 0.164792670886591 1103 76-77 0.18887846483179516 1103 78-79 0.1757986708120427 1103 80-81 0.11628574550918813 1103 82-83 0.22429078190908314 1103 84-85 0.2760159887566829 1103 86-87 0.21841542889180232 1103 88-89 0.18594304217604218 1103 90-91 0.1805021821474284 1103 92-93 0.15707411306675212 1103 94-95 0.29083079546180457 1103 96-97 0.337662823540299 1103 98-99 0.2947908061304325 1103 100 0.3656656499642459 1104 1 0.13867253712540162 1104 2 0.20930855512894908 1104 3 0.18087353941248807 1104 4 0.09495930226775329 1104 5 0.14001304879288057 1104 6 0.12570600224139383 1104 7 0.1416553071143838 1104 8 0.12248883302234503 1104 9 0.1502610487101208 1104 10-11 0.15799126046937317 1104 12-13 0.14445830692439188 1104 14-15 0.20562612338770236 1104 16-17 0.18277089050704376 1104 18-19 0.27462196051259014 1104 20-21 0.22091965945921999 1104 22-23 0.26332248112000656 1104 24-25 0.2554104802038566 1104 26-27 0.23737864257603292 1104 28-29 0.27356364637921615 1104 30-31 0.25106487519889953 1104 32-33 0.1365286826721288 1104 34-35 0.11550617722739531 1104 36-37 0.05586717155069465 1104 38-39 0.06855899558250655 1104 40-41 0.0687008022151332 1104 42-43 0.10192313440638401 1104 44-45 0.2119644378266301 1104 46-47 0.05348022532420771 1104 48-49 -0.20696620722176107 1104 50-51 -0.05900162827261113 1104 52-53 -0.1399045471193574 1104 54-55 -0.10128159697191563 1104 56-57 -0.11184744280063796 1104 58-59 -0.09640241103961245 1104 60-61 -0.04397337640463661 1104 62-63 -0.023387751811355884 1104 64-65 -0.09228601997401142 1104 66-67 -0.21556988118763343 1104 68-69 -0.10703674250668627 1104 70-71 -0.24129304090845238 1104 72-73 -0.1944972755614316 1104 74-75 -0.2637228130974236 1104 76-77 -0.24397272467680153 1104 78-79 -0.2736387694169693 1104 80-81 -0.3345149482036973 1104 82-83 -0.2925606778224257 1104 84-85 -0.25425161637164706 1104 86-87 -0.10369186649350581 1104 88-89 -0.23104284289899724 1104 90-91 -0.1427108105079995 1104 92-93 -0.0790194347278721 1104 94-95 -0.08233622763169635 1104 96-97 -0.051305114933320795 1104 98-99 -0.13407538708317546 1104 100 -0.06148394164026527 1105 1 0.07880053870855619 1105 2 0.08280766985213006 1105 3 0.09979337675942901 1105 4 0.05701608291849425 1105 5 0.11161672662707645 1105 6 0.14651038230024938 1105 7 0.13821614534634818 1105 8 0.08868390175810248 1105 9 0.16481976707515855 1105 10-11 0.18281948978081175 1105 12-13 0.13582292253735062 1105 14-15 0.19454004889451681 1105 16-17 0.18430182222404312 1105 18-19 0.2053454887867261 1105 20-21 0.2820190018607107 1105 22-23 0.2521696922856407 1105 24-25 0.261522463348868 1105 26-27 0.17285762905775925 1105 28-29 0.2414917887870942 1105 30-31 0.17366499543463476 1105 32-33 0.14164070029870857 1105 34-35 0.13887093876235923 1105 36-37 0.21320307551002315 1105 38-39 0.17167612947444155 1105 40-41 0.22458773065188353 1105 42-43 0.16828341483648757 1105 44-45 0.1926997348103896 1105 46-47 0.20262700636973818 1105 48-49 0.08661237502602859 1105 50-51 0.05434004060536779 1105 52-53 0.11002486560953173 1105 54-55 0.02570727747469448 1105 56-57 0.1536558956649543 1105 58-59 0.17121379809659487 1105 60-61 0.2283110875493719 1105 62-63 0.29651625949459515 1105 64-65 0.29225919061093464 1105 66-67 0.205613746163543 1105 68-69 0.14197416116530093 1105 70-71 0.1395054223129577 1105 72-73 0.10006927861536496 1105 74-75 0.07675869953407499 1105 76-77 4.2689372715898344E-4 1105 78-79 0.047524069045870476 1105 80-81 -0.02172463462630958 1105 82-83 0.07676116965010493 1105 84-85 0.1719928059846083 1105 86-87 0.12375831138641757 1105 88-89 0.05270724268025617 1105 90-91 0.16083344508738406 1105 92-93 0.08614553459602448 1105 94-95 0.1094442656946164 1105 96-97 0.2065238254980173 1105 98-99 0.2091881385623715 1105 100 0.15526720487192236 1106 1 0.04069354178684392 1106 2 0.07271607699309612 1106 3 0.12269089158744606 1106 4 0.071592828159325 1106 5 0.018524617219597417 1106 6 0.03132847745435896 1106 7 -0.03744392377875272 1106 8 0.04616174546929841 1106 9 0.1104225959128371 1106 10-11 0.13177778374307536 1106 12-13 0.04496499015899502 1106 14-15 0.1597262233143084 1106 16-17 0.1909679569437941 1106 18-19 0.0889898010843524 1106 20-21 0.05296266168345909 1106 22-23 0.09302937448935467 1106 24-25 0.062204755256139777 1106 26-27 0.07904319173860586 1106 28-29 0.07933197233677447 1106 30-31 0.09390445553651716 1106 32-33 0.12941852007229215 1106 34-35 0.08540677448595346 1106 36-37 0.022100447552290348 1106 38-39 0.006989565193556757 1106 40-41 0.07648881092407578 1106 42-43 0.08790623647889362 1106 44-45 0.09523586170492848 1106 46-47 0.10839751047343071 1106 48-49 0.3245410297462925 1106 50-51 0.247065106643781 1106 52-53 0.2196038296289231 1106 54-55 0.1999520878426182 1106 56-57 0.2513451462777425 1106 58-59 0.2898386593354516 1106 60-61 0.34868997373653343 1106 62-63 0.29508070276315834 1106 64-65 0.38378275655904304 1106 66-67 0.27617650400031124 1106 68-69 0.3606883334909057 1106 70-71 0.31188421890906426 1106 72-73 0.31732549644482333 1106 74-75 0.34769046054432806 1106 76-77 0.29967992827432255 1106 78-79 0.22800945660419103 1106 80-81 0.3007597814289653 1106 82-83 0.2980951805158156 1106 84-85 0.19776843357696805 1106 86-87 0.25576583703535505 1106 88-89 0.24290696074839246 1106 90-91 0.24753589218316563 1106 92-93 0.3272840689702967 1106 94-95 0.26357728321946894 1106 96-97 0.2347310104209619 1106 98-99 0.19162269159397738 1106 100 0.2127384692397385 1107 1 -0.009568174678122432 1107 2 -0.14638884509972883 1107 3 -0.1180654651978088 1107 4 -0.046183379572902084 1107 5 -0.04885312814433718 1107 6 -0.041519104042400556 1107 7 -0.03374418739446838 1107 8 -0.025151243112418342 1107 9 -0.06578209751044284 1107 10-11 -0.011395177033648451 1107 12-13 -0.0028694865220231236 1107 14-15 -0.02201288069613838 1107 16-17 -0.022792479427579337 1107 18-19 0.03850777177515852 1107 20-21 0.006351301137691223 1107 22-23 0.0107159376849566 1107 24-25 -0.024545326411562485 1107 26-27 0.027911619388632403 1107 28-29 0.04818049586087625 1107 30-31 0.005680556910810708 1107 32-33 0.059157715246684006 1107 34-35 0.05414514158792372 1107 36-37 0.07242293997429528 1107 38-39 -0.02337942083557465 1107 40-41 0.01926824737901711 1107 42-43 -0.026234809323391062 1107 44-45 0.020557404502589804 1107 46-47 -0.07554852047672966 1107 48-49 -0.09936747205039609 1107 50-51 0.005795952721072695 1107 52-53 -0.029007819602639984 1107 54-55 0.03769927505535975 1107 56-57 -0.06011643951892154 1107 58-59 -0.10969488519433668 1107 60-61 -0.06643260377180127 1107 62-63 -0.08070083805928618 1107 64-65 0.017732965856247063 1107 66-67 0.12890496462129164 1107 68-69 0.1752988735612533 1107 70-71 0.0102189576863978 1107 72-73 0.058245188614606036 1107 74-75 -0.016558842431415144 1107 76-77 -0.06890346013722848 1107 78-79 0.04236929585178473 1107 80-81 0.05919814321305239 1107 82-83 -0.0847591623061632 1107 84-85 -0.10216851136760852 1107 86-87 -0.06953747297520252 1107 88-89 -0.0629670354986871 1107 90-91 -0.13108312613319129 1107 92-93 -0.13563879874323703 1107 94-95 -0.08230248674353291 1107 96-97 -0.12361542772810452 1107 98-99 -0.2818599986537613 1107 100 -0.39221021306154213 1108 1 0.012995603338403328 1108 2 0.005111961721638636 1108 3 0.051549480689445204 1108 4 0.008082055723598103 1108 5 0.013552824215345538 1108 6 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-0.5511498748474395 2214 88-89 -0.5851151397576899 2214 90-91 -0.4638730611378996 2214 92-93 -0.5319917507252434 2214 94-95 -0.5403901592493909 2214 96-97 -0.46171754363187034 2214 98-99 -0.5397130109666683 2214 100 -0.5780661284614084 2215 1 -0.25545321008949173 2215 2 -0.11613742574114383 2215 3 -0.23331869591513055 2215 4 -0.12588454686225248 2215 5 -0.18172942755225563 2215 6 -0.20448193385487912 2215 7 -0.2274389305825153 2215 8 -0.11323720383773406 2215 9 -0.18561488559253547 2215 10-11 -0.2869361994696078 2215 12-13 -0.18770257188818817 2215 14-15 -0.3154007990716181 2215 16-17 -0.3776260763988759 2215 18-19 -0.37298782288976895 2215 20-21 -0.320221469854026 2215 22-23 -0.2964291466192819 2215 24-25 -0.2696696948557573 2215 26-27 -0.30256023956322053 2215 28-29 -0.3883602445009018 2215 30-31 -0.465119579701593 2215 32-33 -0.48917289643948436 2215 34-35 -0.42552114667530105 2215 36-37 -0.38463835170423977 2215 38-39 -0.4238359247196186 2215 40-41 -0.5185125823615664 2215 42-43 -0.606602273172264 2215 44-45 -0.6390125844633232 2215 46-47 -0.7231521343448151 2215 48-49 -0.9043731481020245 2215 50-51 -0.8812563236452604 2215 52-53 -0.8691652626278028 2215 54-55 -0.8477962352638571 2215 56-57 -0.8781545651636122 2215 58-59 -0.8393148524516008 2215 60-61 -0.7540949001322161 2215 62-63 -0.7060558505452263 2215 64-65 -0.7846631783002493 2215 66-67 -0.6912176062913318 2215 68-69 -0.7297930148701113 2215 70-71 -0.7819374787210833 2215 72-73 -0.7977068532429925 2215 74-75 -0.7584777281277653 2215 76-77 -0.7818587541442596 2215 78-79 -0.879319685556208 2215 80-81 -0.8417924349560693 2215 82-83 -0.8637384823967622 2215 84-85 -0.9512192418567871 2215 86-87 -0.8510461273983765 2215 88-89 -0.9565519564211264 2215 90-91 -0.9876089953779754 2215 92-93 -0.9196577751457973 2215 94-95 -0.9192212618225533 2215 96-97 -0.969807564495369 2215 98-99 -0.8908016466307096 2215 100 -0.8564134087961364 2216 1 -0.21235780926600967 2216 2 -0.177921669519165 2216 3 -0.230555689363932 2216 4 -0.16057215625730237 2216 5 -0.14040726848976703 2216 6 -0.18607439247229252 2216 7 -0.16103508987838921 2216 8 -0.15032108588277282 2216 9 -0.24946409128024527 2216 10-11 -0.252909127839807 2216 12-13 -0.3126796510193586 2216 14-15 -0.38263812591487323 2216 16-17 -0.3115431278583145 2216 18-19 -0.3206313492029267 2216 20-21 -0.29311129132128855 2216 22-23 -0.2771631145133924 2216 24-25 -0.2772575442948195 2216 26-27 -0.3426821785853704 2216 28-29 -0.358481850128598 2216 30-31 -0.2530515338931849 2216 32-33 -0.282692333336243 2216 34-35 -0.30194224054250896 2216 36-37 -0.20304360099994767 2216 38-39 -0.2424412106887175 2216 40-41 -0.2124293837751594 2216 42-43 -0.08509833078282725 2216 44-45 -0.11452684541117009 2216 46-47 0.016292805560823354 2216 48-49 0.12690341926866466 2216 50-51 -0.0333248278710343 2216 52-53 -0.11781188950939736 2216 54-55 -0.025175629819528922 2216 56-57 0.010240598949806667 2216 58-59 0.09606288346664371 2216 60-61 0.08188127883392582 2216 62-63 -0.14746385868004808 2216 64-65 -0.1851376402849496 2216 66-67 -0.20129966300172697 2216 68-69 -0.15916548315595946 2216 70-71 -0.15380357083797236 2216 72-73 -0.049939554238179085 2216 74-75 -0.2344993737627945 2216 76-77 -0.18848017640500103 2216 78-79 -0.23849386748955936 2216 80-81 -0.20440162164028663 2216 82-83 -0.25556240011501075 2216 84-85 -0.3657514894355103 2216 86-87 -0.33951901409427165 2216 88-89 -0.48002500981424845 2216 90-91 -0.4328302025993018 2216 92-93 -0.28932033828236925 2216 94-95 -0.2713962439026041 2216 96-97 -0.3811986111872656 2216 98-99 -0.597890654615508 2216 100 -0.5983848535610115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 10.0 5 20.0 6 65.0 7 147.0 8 248.0 9 520.0 10 858.0 11 1326.0 12 2066.0 13 2986.0 14 3495.0 15 4730.0 16 5936.0 17 8817.0 18 10144.0 19 4890.0 20 2915.0 21 2802.0 22 2760.0 23 2984.0 24 3414.0 25 4219.0 26 5500.0 27 7043.0 28 7544.0 29 9605.0 30 12126.0 31 15563.0 32 19866.0 33 26122.0 34 36167.0 35 58318.0 36 116107.0 37 255280.0 38 307025.0 39 71749.0 40 126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59833494232314 17.764234624276202 13.252153381148958 24.3852770522517 2 17.730012442461035 19.83395545638512 36.217254469098584 26.21877763205526 3 19.32880237441192 23.279972214328566 29.52188121625462 27.869344195004892 4 14.263428823461503 16.74726411063738 34.21294893894559 34.77635812695553 5 14.846699253476572 35.85631150727481 33.32053935972247 15.976449879526156 6 32.79348791317218 34.92096694622595 16.321854958066105 15.963690182535768 7 29.523630981746425 31.517809570794274 20.101036013813516 18.85752343364578 8 27.285527238857142 32.47291309199731 19.341547138274706 20.90001253087084 9 26.80424272323631 14.735865811544155 19.602466699555997 38.85742476566354 10-11 26.07890430476367 24.598431070352202 26.562873861549186 22.759790763334937 12-13 26.95046867291564 22.671040947212628 26.771682289097186 23.606808090774546 14-15 24.30486933107317 23.939500531082246 24.82899546273717 26.926634675107415 16-17 23.302622848107667 26.739039810162335 25.824385358622 24.133951983108 18-19 23.116971149790306 24.755412836885483 28.903836087749944 23.223779925574263 20-21 24.15353929627 24.110273364713056 28.574409755505553 23.161777583511387 22-23 23.991970395644007 24.021028130748952 28.49868204177703 23.48831943183001 24-25 23.88934030136593 24.000146030702954 28.52418707519743 23.586326592733688 26-27 23.619066225028345 24.057448502662556 28.893269751622842 23.430215520686257 28-29 23.519025472707604 24.04744367642118 29.009078965738905 23.42445188513231 30-31 23.79321300408388 24.12705711018385 28.36387280870096 23.71585707703131 32-33 23.679329057720768 24.145633941785892 28.5007400098668 23.67429699062654 34-35 23.881450419338922 23.93330044400592 28.419486926492354 23.7657622101628 36-37 24.077947705969414 24.00404538727183 28.32002960039467 23.597977306364086 38-39 23.776911692155895 24.346817957572767 28.241440552540702 23.634829797730635 40-41 23.77351937519456 23.98851701456192 28.24283583761018 23.99512777263334 42-43 23.968426245683276 24.036507153428712 27.834435125801676 24.160631475086333 44-45 23.985841144548594 24.288258510113465 27.467488899852 24.25841144548594 46-47 24.75742476566354 24.299407992106563 26.93754316724223 24.005624074987665 48-49 24.428712382831772 24.6 26.44518993586581 24.526097681302417 50-51 24.12121361618155 24.959447459299458 26.49689195855945 24.422446965959548 52-53 24.297582634435123 24.944104588061176 26.339516526887024 24.418796250616676 54-55 24.05402072027627 24.651652688702516 26.34588061174149 24.948445979279725 56-57 24.013320177602367 24.875974346324618 26.274494326591018 24.836211149481993 58-59 24.178095707942774 24.481055747409965 26.559792797237296 24.781055747409965 60-61 23.951603354711395 24.81302417365565 26.32590034533794 24.909472126295018 62-63 23.735125801677356 24.764824864331526 26.67069560927479 24.82935372471633 64-65 23.876369018253577 24.915737543167243 26.4486433152442 24.75925012333498 66-67 23.760779477059693 24.95076467686236 26.12200296003947 25.16645288603848 68-69 23.58216436326035 24.81645270493607 26.450642773204514 25.15074015859907 70-71 23.8858035943777 24.579883857860807 26.577714147860952 24.956598399900543 72-73 23.865536849147063 24.53268238583834 26.33653577662292 25.26524498839168 74-75 23.88902209079634 24.896867189804826 25.837815330848873 25.376295388549963 76-77 23.978786383818452 24.343117908238774 26.098618648248646 25.579477059694128 78-79 23.889886531820427 24.677257030093735 25.886630488406514 25.54622594967933 80-81 23.860335471139614 24.69610261470153 26.007301430685743 25.43626048347311 82-83 23.787739307093545 24.50569758825613 26.070820000809082 25.635743103841246 84-85 23.792895905278737 24.62950172668969 25.69580661075481 25.881795757276766 86-87 23.751673660602844 24.57945114785737 26.125237667205724 25.543637524334063 88-89 23.292253347410384 24.608819205304545 26.024055966136146 26.074871481148925 90-91 23.58177984372518 24.25693483089304 26.032910660492504 26.128374664889275 92-93 23.597104272057134 24.34279269812421 26.181532580886103 25.878570448932553 94-95 23.21840600180267 24.12955952937137 26.34945098424216 26.302583484583796 96-97 23.513726449791044 24.059903334930112 26.04554812311205 26.380822092166795 98-99 23.076133732675743 24.397051996572273 26.22650529181317 26.300308978938812 100 23.240157868771583 24.062358164775528 26.151850024666995 26.54563394178589 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46.0 1 38.0 2 100.5 3 176.0 4 124.5 5 89.5 6 173.5 7 369.5 8 600.5 9 716.5 10 597.0 11 417.5 12 390.0 13 429.5 14 485.0 15 547.5 16 666.5 17 783.5 18 847.0 19 960.5 20 1099.0 21 1232.5 22 1358.0 23 1509.5 24 1796.0 25 2263.5 26 2780.5 27 3286.5 28 3895.5 29 4637.0 30 5431.5 31 6297.0 32 7317.5 33 8812.5 34 10785.5 35 12492.0 36 14283.5 37 16826.5 38 19257.0 39 21203.5 40 24449.5 41 27784.0 42 28218.5 43 29139.0 44 31679.5 45 32661.0 46 35346.5 47 38106.5 48 39561.5 49 40674.5 50 40881.0 51 41791.0 52 41792.0 53 43536.0 54 43937.0 55 41740.5 56 39273.5 57 34670.0 58 30521.5 59 28658.5 60 26897.5 61 22494.0 62 18880.0 63 16541.5 64 13261.5 65 11219.0 66 8872.0 67 6261.0 68 5075.5 69 4150.0 70 3086.5 71 2144.0 72 1602.0 73 1223.5 74 906.5 75 559.0 76 302.0 77 220.0 78 146.5 79 74.0 80 39.0 81 8.0 82 5.0 83 3.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04361124814997533 2 0.24134188455846076 3 0.0027627035027133697 4 0.0026640355204736064 5 1.9733596447952636E-4 6 0.0 7 0.0 8 2.960039467192896E-4 9 0.0 10-11 6.413418845584608E-4 12-13 0.0 14-15 3.453379378391712E-4 16-17 2.4666995559940796E-4 18-19 6.413418845584608E-4 20-21 4.933399111988159E-5 22-23 4.933399111988159E-5 24-25 0.0014306857424765665 26-27 9.866798223976318E-5 28-29 3.453379378391712E-4 30-31 2.960039467192896E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.933399111988159E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.866798223976318E-5 70-71 4.933399111988159E-5 72-73 2.960039467192896E-4 74-75 9.866798223976318E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.866798223976318E-5 84-85 0.0 86-87 2.960039467192896E-4 88-89 0.0029600394671928957 90-91 0.002861371484953133 92-93 6.906758756783424E-4 94-95 4.4400592007893435E-4 96-97 4.933399111988159E-5 98-99 4.933399111988159E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1013500.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.15734237564259 #Duplication Level Percentage of deduplicated Percentage of total 1 57.16507165196945 13.237911361727969 2 11.936685960248388 5.528438472239959 3 6.300881984574366 4.377350441559209 4 4.195964162540486 3.8866951483150585 5 3.0679555273835803 3.5522848270433354 6 2.3761627230619484 3.301536823091085 7 1.8731356172574352 3.0363789963388728 8 1.5412019534575065 2.8552113044979723 9 1.2853987708328656 2.6789777482866133 >10 9.522402425610883 42.129736384756704 >50 0.5941594835423953 9.196342276913558 >100 0.1371464535518736 4.847856822957538 >500 0.0017036826528178688 0.27567617790409055 >1k 0.002129603316022336 1.0956032143680279 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3354 0.33093241243216576 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3350 0.33053774050320667 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1664 0.16418352244696596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1573 0.15520473606314752 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1167 0.11514553527380365 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.86679822397632E-5 0.0 20-21 0.0 0.0 0.0 2.960039467192896E-4 0.0 22-23 0.0 0.0 0.0 8.386778490379872E-4 0.0 24-25 0.0 0.0 0.0 0.0023680315737543167 0.0 26-27 0.0 0.0 0.0 0.004834731129748396 0.0 28-29 0.0 0.0 0.0 0.012234829797730636 0.0 30-31 0.0 0.0 0.0 0.03285643808584115 0.0 32-33 0.0 0.0 0.0 0.08001973359644796 0.0 34-35 0.0 0.0 0.0 0.1614208189442526 0.0 36-37 0.0 0.0 0.0 0.2758756783423779 0.0 38-39 0.0 0.0 0.0 0.4218056240749877 0.0 40-41 0.0 0.0 0.0 0.5747409965466206 0.0 42-43 0.0 0.0 0.0 0.7208189442525901 0.0 44-45 0.0 0.0 0.0 0.9079921065614208 0.0 46-47 0.0 0.0 0.0 1.1278243709916134 0.0 48-49 0.0 0.0 0.0 1.3424765663542182 0.0 50-51 0.0 0.0 0.0 1.5530340404538727 0.0 52-53 0.0 0.0 0.0 1.7737543167242231 0.0 54-55 0.0 0.0 0.0 2.024617661568821 0.0 56-57 0.0 0.0 0.0 2.319585594474593 0.0 58-59 0.0 0.0 0.0 2.6193882585101136 0.0 60-61 0.0 0.0 0.0 2.9255550074000984 0.0 62-63 0.0 0.0 0.0 3.2407498766650225 0.0 64-65 0.0 0.0 0.0 3.5873704982733106 0.0 66-67 0.0 0.0 0.0 3.9857918105574743 0.0 68-69 0.0 0.0 0.0 4.427479033053775 0.0 70-71 0.0 0.0 0.0 4.8861371484953136 0.0 72-73 0.0 0.0 0.0 5.361519486926492 0.0 74-75 0.0 0.0 0.0 5.877010360138135 0.0 76-77 0.0 0.0 0.0 6.443611248149975 0.0 78-79 0.0 0.0 0.0 7.0542180562407495 0.0 80-81 0.0 0.0 0.0 7.707202762703503 0.0 82-83 0.0 0.0 0.0 8.361026147015293 0.0 84-85 0.0 0.0 0.0 9.046127281697089 0.0 86-87 0.0 0.0 0.0 9.773359644795264 0.0 88 0.0 0.0 0.0 10.360039467192896 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3675 0.0 38.617847 1 GTATCAA 5565 0.0 28.891369 1 CCGTTAA 155 4.9278242E-8 27.377243 1 CGTTAAT 155 4.9538357E-8 27.362349 2 GTCGTAA 90 9.341863E-4 26.194277 1 TCAACGC 6285 0.0 24.97613 4 GTATATA 95 0.0012805243 24.81563 1 TATCAAC 6610 0.0 24.310587 2 ATCAACG 6490 0.0 24.187208 3 AACGCAG 6585 0.0 23.837088 6 GTACATG 3725 0.0 23.796358 1 CAACGCA 6625 0.0 23.764105 5 CGTAAAC 100 0.0017588641 23.499247 3 CGACCGT 70 1.9331073E-5 23.498667 18-19 TACATGG 3785 0.0 23.157389 2 ATTAACG 205 2.903107E-8 22.987337 2 CTATACA 150 3.2916905E-5 21.93263 4 ACATGGG 4020 0.0 21.161015 3 CCGGTGC 135 3.990007E-4 20.955421 1 ACGCAGA 7500 0.0 20.928963 7 >>END_MODULE