FastQCFastQC Report
Wed 25 May 2016
SRR1033094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455964
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40300.035.8172451
TCAACGC37750.035.7389644
ATCAACG39150.034.460943
CAACGCA40400.033.6274155
AACGCAG43200.031.1151036
GGTATCA25450.030.482811
TATCAAC48600.028.727462
GTACATG91150.027.6997871
TCGTACG856.811612E-427.65212
TACATGG91600.026.2755322
ACGCAGA52000.025.487947
CGCAGAG53050.024.4674978
ACATGGG97350.023.468073
TATACCG2109.5225187E-720.146535
CGTACGC1200.00506081119.5869053
CATGGGG66550.019.425034
GAGTACA65600.019.4233551
TACACCG4950.018.993365
GCAGAGT67250.018.6053129
AGTACAT65250.017.8668582
Produced by FastQC (version 0.11.5)