FastQCFastQC Report
Wed 25 May 2016
SRR1033094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455964
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53790.15564398240259447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.13148285109451371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49750.050.590791
GTATCAA88250.040.697111
TCAACGC102950.034.4670644
ATCAACG103050.034.343893
CAACGCA106100.033.3542145
AACGCAG109050.032.4950186
TATCAAC112600.031.7894882
ACGCAGA125550.028.0373027
CGCAGAG126600.027.730528
GTACATG85600.027.2004761
GTGGTAT23650.025.7088031
TACATGG87450.025.6901662
TCGTACG950.001289266524.7887552
GCAGAGT143350.024.2935879
ACATGGG93050.023.0330263
TGGTATC26500.021.3276822
GAGTACT101900.021.26215612-13
CAGAGTA143850.020.25596610-11
AGAGTAC131800.020.25368310-11
GAGTACA63150.020.002521
Produced by FastQC (version 0.11.5)