##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033094_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3455964 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68880607552625 34.0 31.0 34.0 31.0 34.0 2 32.69372076792467 34.0 31.0 34.0 31.0 34.0 3 32.79577622915053 34.0 31.0 34.0 31.0 34.0 4 36.283922807066276 37.0 37.0 37.0 35.0 37.0 5 36.267683054568856 37.0 37.0 37.0 35.0 37.0 6 36.33459434183921 37.0 37.0 37.0 35.0 37.0 7 36.28304519375781 37.0 37.0 37.0 35.0 37.0 8 36.29441828676456 37.0 37.0 37.0 35.0 37.0 9 38.09527992768443 39.0 39.0 39.0 35.0 39.0 10-11 38.05563700895033 39.0 39.0 39.0 35.0 39.0 12-13 38.02637426200042 39.0 38.0 39.0 35.0 39.0 14-15 39.51136542510281 41.0 39.0 41.0 37.0 41.0 16-17 39.51362123563787 41.0 39.0 41.0 37.0 41.0 18-19 39.530848990325126 41.0 39.5 41.0 36.5 41.0 20-21 39.527398144193626 41.0 39.5 41.0 37.0 41.0 22-23 39.45438494150981 41.0 39.0 41.0 36.0 41.0 24-25 39.40020005995433 41.0 39.0 41.0 36.0 41.0 26-27 39.290970768214024 41.0 39.0 41.0 36.0 41.0 28-29 39.23998412599205 41.0 39.0 41.0 36.0 41.0 30-31 39.238621119895924 41.0 39.0 41.0 36.0 41.0 32-33 39.199482112660895 41.0 39.0 41.0 35.0 41.0 34-35 39.118890272005146 41.0 39.0 41.0 35.0 41.0 36-37 39.02888687497902 41.0 39.0 41.0 35.0 41.0 38-39 38.936530444182864 41.0 39.0 41.0 35.0 41.0 40-41 38.833895405160476 41.0 39.0 41.0 35.0 41.0 42-43 38.71753177984493 41.0 39.0 41.0 35.0 41.0 44-45 38.551813763106324 41.0 38.0 41.0 35.0 41.0 46-47 38.35556634849206 40.5 38.0 41.0 34.5 41.0 48-49 38.15538544382986 40.0 38.0 41.0 34.0 41.0 50-51 38.019823123157536 40.0 38.0 41.0 34.0 41.0 52-53 37.88956945153364 40.0 38.0 41.0 33.5 41.0 54-55 37.74334686356687 40.0 37.5 41.0 33.0 41.0 56-57 37.59657681619369 40.0 37.0 41.0 33.0 41.0 58-59 37.42244956834041 40.0 37.0 41.0 33.0 41.0 60-61 37.23221133090507 40.0 36.0 41.0 33.0 41.0 62-63 37.001743218390004 39.0 36.0 41.0 32.5 41.0 64-65 36.73993291018078 39.0 35.0 41.0 32.0 41.0 66-67 36.464791733941674 39.0 35.0 41.0 32.0 41.0 68-69 36.15832384249373 38.0 35.0 40.5 31.0 41.0 70-71 35.83296889087965 37.0 35.0 40.0 31.0 41.0 72-73 35.49114197948821 37.0 35.0 39.5 31.0 41.0 74-75 35.14477856250817 36.0 35.0 39.0 31.0 41.0 76-77 34.78922899659834 36.0 35.0 39.0 30.5 40.5 78-79 34.424862787922564 35.5 34.5 37.5 30.0 39.5 80-81 34.089601049085005 35.0 34.0 37.0 30.0 39.0 82-83 33.78540893365788 35.0 34.0 37.0 30.0 39.0 84-85 33.55459099110986 35.0 34.0 36.0 30.0 37.5 86-87 33.339566326501085 35.0 34.0 36.0 30.0 37.0 88-89 33.12349636743901 35.0 34.0 36.0 30.0 37.0 90-91 32.93795638496235 35.0 34.0 35.0 29.0 36.0 92-93 32.75747099796179 35.0 34.0 35.0 29.0 36.0 94-95 32.598968478838316 35.0 34.0 35.0 29.0 36.0 96-97 32.45121679508236 35.0 34.0 35.0 29.0 36.0 98-99 32.32588606247056 35.0 34.0 35.0 29.0 35.5 100 32.2194397858311 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0954551457903392 1101 2 0.11637598923396553 1101 3 0.13140395167354058 1101 4 0.10619115627024911 1101 5 0.10466353127209516 1101 6 0.11686257228656416 1101 7 0.13512394610102518 1101 8 0.1704953936243072 1101 9 0.20154289971399209 1101 10-11 0.19953325684885215 1101 12-13 0.21002793805295283 1101 14-15 0.2847857408295553 1101 16-17 0.3349695006167508 1101 18-19 0.31169101029788493 1101 20-21 0.26504316162367303 1101 22-23 0.28367665644706364 1101 24-25 0.3388474270119417 1101 26-27 0.32350899885675233 1101 28-29 0.3304121413194565 1101 30-31 0.3426211569456825 1101 32-33 0.3498701479734194 1101 34-35 0.34697050203227775 1101 36-37 0.2885509579551453 1101 38-39 0.32367404956703894 1101 40-41 0.33198909491028417 1101 42-43 0.2796793503359538 1101 44-45 0.24986193509536747 1101 46-47 0.2639985193046215 1101 48-49 0.2600203482395145 1101 50-51 0.2960174366786532 1101 52-53 0.25857700617508783 1101 54-55 0.3163222229975631 1101 56-57 0.2509072724144801 1101 58-59 0.2322099781582594 1101 60-61 0.20680486583759006 1101 62-63 0.25388971608019517 1101 64-65 0.2855520732792982 1101 66-67 0.305447680934698 1101 68-69 0.23907030443624677 1101 70-71 0.17383133309628107 1101 72-73 0.20510223126078841 1101 74-75 0.20565165006623687 1101 76-77 0.2320344027560992 1101 78-79 0.25575148073934173 1101 80-81 0.24917853702815052 1101 82-83 0.2544144908718877 1101 84-85 0.3259545414657907 1101 86-87 0.3388525564476481 1101 88-89 0.3280461570770896 1101 90-91 0.32286836810670394 1101 92-93 0.2802833650508063 1101 94-95 0.3140304086162615 1101 96-97 0.31120646807178076 1101 98-99 0.32594240015425413 1101 100 0.2947222247702399 1102 1 0.12151433876655915 1102 2 0.1082362051986081 1102 3 0.1590965921948353 1102 4 0.08831504330282769 1102 5 0.08092201265242949 1102 6 0.08700635534675172 1102 7 0.11067855059724963 1102 8 0.16166141671192236 1102 9 0.20383203915645964 1102 10-11 0.17548161495366088 1102 12-13 0.18897493014863898 1102 14-15 0.2600143797668082 1102 16-17 0.27227735416786203 1102 18-19 0.2596318096120669 1102 20-21 0.22887332463560028 1102 22-23 0.24292311094873043 1102 24-25 0.27330627858459877 1102 26-27 0.2808956068903612 1102 28-29 0.2504384141247087 1102 30-31 0.2106623869349349 1102 32-33 0.2046729877003699 1102 34-35 0.16613908677481248 1102 36-37 0.14900773000480427 1102 38-39 0.2148437441155906 1102 40-41 0.27714336819465757 1102 42-43 0.27794652419387944 1102 44-45 0.2105341480968903 1102 46-47 0.1303721256655308 1102 48-49 0.10855333398907163 1102 50-51 0.05718314386079726 1102 52-53 0.056020484715560315 1102 54-55 0.06679351917746601 1102 56-57 0.06968374888943174 1102 58-59 -0.02435852871734312 1102 60-61 0.034015595378974695 1102 62-63 0.040167510943405205 1102 64-65 0.02272396055031578 1102 66-67 0.001961758049297657 1102 68-69 0.02706515367271578 1102 70-71 0.001702502519115967 1102 72-73 -0.05190464146964047 1102 74-75 -0.02631903221342924 1102 76-77 -0.0075982330247370555 1102 78-79 0.035157085786231335 1102 80-81 0.03593324573557055 1102 82-83 -0.03474885604507705 1102 84-85 0.028187682902220956 1102 86-87 0.06955823004415151 1102 88-89 0.08058480073129459 1102 90-91 0.007030470675758238 1102 92-93 0.05333388745598455 1102 94-95 0.053616199188041946 1102 96-97 0.05497323468429727 1102 98-99 0.05976246923412987 1102 100 0.08457833076705157 1103 1 0.13792739868159032 1103 2 0.1642359903938626 1103 3 0.12860772080855298 1103 4 0.08973255872432873 1103 5 0.11413788911013256 1103 6 0.10620096755916819 1103 7 0.13434986477662392 1103 8 0.1470735292418226 1103 9 0.21672014600881795 1103 10-11 0.23198891199211857 1103 12-13 0.24362301282032917 1103 14-15 0.2974673066240072 1103 16-17 0.2941886186108462 1103 18-19 0.2770653924644506 1103 20-21 0.28822702950990475 1103 22-23 0.2624024596806791 1103 24-25 0.2818794549572843 1103 26-27 0.2810460013848015 1103 28-29 0.3127435914713246 1103 30-31 0.26993151021272865 1103 32-33 0.2291745106589218 1103 34-35 0.26688723426195793 1103 36-37 0.28273969446548364 1103 38-39 0.2947471256161336 1103 40-41 0.26908707688237143 1103 42-43 0.22453410317157818 1103 44-45 0.1821520686907263 1103 46-47 0.20322798330733605 1103 48-49 0.12052061687464288 1103 50-51 0.15667071608800143 1103 52-53 0.19868345535125087 1103 54-55 0.17149351299693194 1103 56-57 0.156109620500672 1103 58-59 0.18523466742237105 1103 60-61 0.24865358614593447 1103 62-63 0.2199614462180648 1103 64-65 0.2074309088071189 1103 66-67 0.15656591795877972 1103 68-69 0.13464058781074328 1103 70-71 0.1384531995213365 1103 72-73 0.2046235482695664 1103 74-75 0.18120927312137525 1103 76-77 0.1360943220670805 1103 78-79 0.1416015221562006 1103 80-81 0.11731151743365587 1103 82-83 0.1993716004381767 1103 84-85 0.17609849743078598 1103 86-87 0.1665504336647956 1103 88-89 0.1728909523581521 1103 90-91 0.12719084041065543 1103 92-93 0.12150505121859823 1103 94-95 0.19994026830957523 1103 96-97 0.17934387101754368 1103 98-99 0.2526442210007076 1103 100 0.21834139827861776 1104 1 0.1073851344972141 1104 2 0.10112534112890614 1104 3 0.121495635091172 1104 4 0.10140084954620932 1104 5 0.11275039894076855 1104 6 0.09562520538572272 1104 7 0.1202452562828853 1104 8 0.1368258226609953 1104 9 0.1398763904188911 1104 10-11 0.15486296579866377 1104 12-13 0.19101345192604668 1104 14-15 0.28477131759155583 1104 16-17 0.2878783004223777 1104 18-19 0.27399260790889457 1104 20-21 0.2725961903155323 1104 22-23 0.31542166578275044 1104 24-25 0.29378712623098124 1104 26-27 0.27727298021512325 1104 28-29 0.3025451017150331 1104 30-31 0.26482861650104894 1104 32-33 0.2549344693439437 1104 34-35 0.24441568073798692 1104 36-37 0.24419676873910845 1104 38-39 0.2105708693057764 1104 40-41 0.18600814361800388 1104 42-43 0.19838308287286566 1104 44-45 0.20901949078474047 1104 46-47 0.18295591270121037 1104 48-49 0.11299286113129625 1104 50-51 0.16929097061026255 1104 52-53 0.2111009026137296 1104 54-55 0.22056294164390522 1104 56-57 0.21295064031400557 1104 58-59 0.18498086134955827 1104 60-61 0.19619379714015395 1104 62-63 0.13389106344354218 1104 64-65 0.12011215824919219 1104 66-67 0.19543855398135435 1104 68-69 0.17606100064147512 1104 70-71 0.12121186702842834 1104 72-73 0.1042309422658576 1104 74-75 0.06977529681265793 1104 76-77 0.03083493582464314 1104 78-79 0.020548244773912927 1104 80-81 0.02009259047583356 1104 82-83 0.05451804255393 1104 84-85 0.14536220864410865 1104 86-87 0.19363254294176357 1104 88-89 0.15264085960989604 1104 90-91 0.18943764954815379 1104 92-93 0.20879024099358645 1104 94-95 0.1725186902281024 1104 96-97 0.17092121763331392 1104 98-99 0.21183855176559518 1104 100 0.19254397540732526 1105 1 -0.02906882668894184 1105 2 0.07575580335399934 1105 3 0.04205759357375882 1105 4 0.03540159017725841 1105 5 0.061325114667354796 1105 6 0.07795346252360247 1105 7 0.033750912915508025 1105 8 0.05974845124654138 1105 9 0.0725085312885767 1105 10-11 0.07987886402338518 1105 12-13 0.10398623678084817 1105 14-15 0.13893092593922773 1105 16-17 0.10515542859339178 1105 18-19 0.15862969584961206 1105 20-21 0.15004329648122905 1105 22-23 0.1639282561238602 1105 24-25 0.14826287145747585 1105 26-27 0.23535261681691821 1105 28-29 0.19380973289450054 1105 30-31 0.12178451727467632 1105 32-33 0.16590231931181165 1105 34-35 0.1714696546118546 1105 36-37 0.15240189348686073 1105 38-39 0.13456446030473757 1105 40-41 0.1595184399573384 1105 42-43 0.16819448837989626 1105 44-45 0.13788940378942982 1105 46-47 0.12321375920311795 1105 48-49 0.207167251233642 1105 50-51 0.17313879180415626 1105 52-53 0.19556064686289432 1105 54-55 0.225671980031386 1105 56-57 0.17690100454966284 1105 58-59 0.19675090864743794 1105 60-61 0.22769275137852674 1105 62-63 0.23434739468156351 1105 64-65 0.190379367016412 1105 66-67 0.20714189331116728 1105 68-69 0.206043602553585 1105 70-71 0.17478299534872832 1105 72-73 0.12852299913183174 1105 74-75 0.08447980427310142 1105 76-77 0.0915545996603413 1105 78-79 0.13348587036664128 1105 80-81 0.17262367963805048 1105 82-83 0.17063043944899192 1105 84-85 0.15971380353923337 1105 86-87 0.17403410744566372 1105 88-89 0.18015922653710703 1105 90-91 0.13981510495981553 1105 92-93 0.15593058310535213 1105 94-95 0.12021533821057773 1105 96-97 0.15987194130003246 1105 98-99 0.08979054688416 1105 100 0.07702660952607943 1106 1 -0.04466183447216565 1106 2 0.018985773212897072 1106 3 0.0019552034070215996 1106 4 0.029511616358398385 1106 5 0.06077730540005177 1106 6 0.017967072341249946 1106 7 0.04624872490562382 1106 8 0.04494190958298816 1106 9 0.0413650294933916 1106 10-11 0.08223117031238303 1106 12-13 0.033482886483099605 1106 14-15 0.059428531080520486 1106 16-17 0.09754853612164993 1106 18-19 0.10760470621621465 1106 20-21 0.061454491261592636 1106 22-23 0.03206056701903037 1106 24-25 0.04343950632164706 1106 26-27 0.0802429001746745 1106 28-29 0.08288517779435267 1106 30-31 0.06163342487752743 1106 32-33 0.056910498227075834 1106 34-35 0.07328156835419719 1106 36-37 0.033454970422987174 1106 38-39 0.08105993225958485 1106 40-41 0.06344593558044664 1106 42-43 0.16876357385727658 1106 44-45 0.1709485954426242 1106 46-47 0.1208882559144584 1106 48-49 0.14594914047529528 1106 50-51 0.0799884135224147 1106 52-53 0.10610620328679943 1106 54-55 0.09708482743798186 1106 56-57 0.08068851391864484 1106 58-59 0.1233371235416385 1106 60-61 0.10156045988509277 1106 62-63 0.131778747405221 1106 64-65 0.1472440637314847 1106 66-67 0.08733590220094811 1106 68-69 0.09784416086761638 1106 70-71 0.1192015343057804 1106 72-73 0.13513240258284753 1106 74-75 0.12033228597005774 1106 76-77 0.12210613583943086 1106 78-79 0.1117515550785626 1106 80-81 0.10827773410491659 1106 82-83 0.1822232878442307 1106 84-85 0.07764867854816515 1106 86-87 0.08058736952926893 1106 88-89 0.12360290539442076 1106 90-91 0.060656105426346585 1106 92-93 0.10177727558611593 1106 94-95 0.16184594385905626 1106 96-97 0.1685865494067329 1106 98-99 0.14300219369412304 1106 100 0.10524732819509808 1107 1 0.03506240638236591 1107 2 -0.04285487500713003 1107 3 -0.008982042769353882 1107 4 0.03269492392917073 1107 5 0.029773528135990546 1107 6 0.008413538055030756 1107 7 -2.0215931085942884E-4 1107 8 0.021573073249783192 1107 9 0.03428391236430173 1107 10-11 0.032164197728207 1107 12-13 0.06111306676992001 1107 14-15 -0.009551959197274584 1107 16-17 0.023300178355903256 1107 18-19 0.021239904969228007 1107 20-21 0.03397126149443608 1107 22-23 0.03899206180082615 1107 24-25 0.0045850518653622885 1107 26-27 0.05971452986128867 1107 28-29 0.05569006317773528 1107 30-31 0.030328191751166855 1107 32-33 0.0961069834728363 1107 34-35 0.10700305497825013 1107 36-37 0.1101047668628965 1107 38-39 0.09904265891237429 1107 40-41 0.0638022836807437 1107 42-43 0.0382155514224749 1107 44-45 0.0580661813605019 1107 46-47 0.059097524805132196 1107 48-49 0.018680187598818065 1107 50-51 0.06338597144984703 1107 52-53 0.08070718333529214 1107 54-55 0.0429609149271073 1107 56-57 0.0694459054837182 1107 58-59 0.10465131926772386 1107 60-61 0.05870294428825673 1107 62-63 0.006871100509243888 1107 64-65 0.03153426637173595 1107 66-67 0.058922286773714916 1107 68-69 0.013064785283681601 1107 70-71 0.03860119195805112 1107 72-73 0.04263221905593184 1107 74-75 -0.013837537015653822 1107 76-77 0.06487820454600524 1107 78-79 0.04722465135899512 1107 80-81 0.047835170365061686 1107 82-83 0.06324512666307669 1107 84-85 0.05670442444431245 1107 86-87 0.04908510293496704 1107 88-89 -0.01709492954810088 1107 90-91 -0.03895668594290669 1107 92-93 -0.01853377748101792 1107 94-95 -0.01527748838619658 1107 96-97 -0.0013781352969672866 1107 98-99 0.06739737668004864 1107 100 0.0549868231585009 1108 1 -0.05248146853918456 1108 2 -0.016666973418956843 1108 3 -0.013433828860577535 1108 4 0.01110787932795887 1108 5 -0.02780688337934123 1108 6 -0.01575394082042436 1108 7 -0.008524810178286657 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2214 54-55 -0.13153476045044243 2214 56-57 -0.1466462147485572 2214 58-59 -0.17024789482871938 2214 60-61 -0.16492808348404964 2214 62-63 -0.19397156745627342 2214 64-65 -0.21332139350527513 2214 66-67 -0.14032590066043582 2214 68-69 -0.13846421987644675 2214 70-71 -0.13587965921804113 2214 72-73 -0.08676744216207055 2214 74-75 -0.13413862292496503 2214 76-77 -0.1680796881263973 2214 78-79 -0.10404512471685479 2214 80-81 -0.17019762916843462 2214 82-83 -0.17372583134184083 2214 84-85 -0.09433387320350306 2214 86-87 -0.1767371533651101 2214 88-89 -0.1334199406297003 2214 90-91 -0.11320081344360489 2214 92-93 -0.14633278271332273 2214 94-95 -0.23238728304876588 2214 96-97 -0.1382543584102578 2214 98-99 -0.18139887638403707 2214 100 -0.1790560517298445 2215 1 -0.19714714104508602 2215 2 -0.1739697132250697 2215 3 -0.23510833064670322 2215 4 -0.16137629481841032 2215 5 -0.18976343409664054 2215 6 -0.18305202193881343 2215 7 -0.20101007081977684 2215 8 -0.13902631259296783 2215 9 -0.2554921709574529 2215 10-11 -0.25414389894653766 2215 12-13 -0.21756121589790922 2215 14-15 -0.34206604639314975 2215 16-17 -0.3829560342087248 2215 18-19 -0.342005279822196 2215 20-21 -0.33566275729534567 2215 22-23 -0.33518554715335824 2215 24-25 -0.40753884201404134 2215 26-27 -0.4492816524691605 2215 28-29 -0.455940844634803 2215 30-31 -0.48865289023446223 2215 32-33 -0.407121576465741 2215 34-35 -0.4616407153998523 2215 36-37 -0.4857941151326415 2215 38-39 -0.49303598177908015 2215 40-41 -0.4545002905548401 2215 42-43 -0.44367598147543674 2215 44-45 -0.41222509536743246 2215 46-47 -0.46493023798739586 2215 48-49 -0.4239798594294726 2215 50-51 -0.44745295772709426 2215 52-53 -0.4405912525398037 2215 54-55 -0.5533218003933555 2215 56-57 -0.4636459068614869 2215 58-59 -0.4339137998927072 2215 60-61 -0.4356724045423874 2215 62-63 -0.4352093463863227 2215 64-65 -0.4803244354770797 2215 66-67 -0.4715828558841082 2215 68-69 -0.43872454696386143 2215 70-71 -0.4224296254725104 2215 72-73 -0.38189993819531054 2215 74-75 -0.360266288722876 2215 76-77 -0.31622646629781315 2215 78-79 -0.35289326326712 2215 80-81 -0.39554092893741455 2215 82-83 -0.4583574183622403 2215 84-85 -0.43578541476045984 2215 86-87 -0.39761047954432627 2215 88-89 -0.35912229976037935 2215 90-91 -0.33878293449046737 2215 92-93 -0.36371946303835045 2215 94-95 -0.3826869305753462 2215 96-97 -0.37519824143478786 2215 98-99 -0.40547387122996525 2215 100 -0.3968610312982861 2216 1 -0.21576349536891115 2216 2 -0.14568205863154304 2216 3 -0.16457848186749402 2216 4 -0.17746267836992047 2216 5 -0.19772824099617736 2216 6 -0.18107558534803303 2216 7 -0.1701202869110645 2216 8 -0.21360452093291826 2216 9 -0.22487094398400842 2216 10-11 -0.25714119129222723 2216 12-13 -0.25271907364534485 2216 14-15 -0.35890293615392466 2216 16-17 -0.3481964108309086 2216 18-19 -0.33751266360822285 2216 20-21 -0.31173096664585387 2216 22-23 -0.3285202991187077 2216 24-25 -0.3489457586410225 2216 26-27 -0.3719885715723308 2216 28-29 -0.33427244686421176 2216 30-31 -0.32792774300857275 2216 32-33 -0.313532233195005 2216 34-35 -0.35950041748999695 2216 36-37 -0.3003344702339419 2216 38-39 -0.3300251487716821 2216 40-41 -0.34223546071945066 2216 42-43 -0.2816561393341459 2216 44-45 -0.3010170930017608 2216 46-47 -0.24409210085662636 2216 48-49 -0.3426825410885428 2216 50-51 -0.3451288455496453 2216 52-53 -0.38254432974432717 2216 54-55 -0.28646155889479274 2216 56-57 -0.29483025489674475 2216 58-59 -0.30691484811564607 2216 60-61 -0.2924941761443236 2216 62-63 -0.3154577629855311 2216 64-65 -0.34987837760893825 2216 66-67 -0.36128962259149944 2216 68-69 -0.3091891949186021 2216 70-71 -0.29270365919098396 2216 72-73 -0.26865624749067507 2216 74-75 -0.37353787961534834 2216 76-77 -0.3253814230758252 2216 78-79 -0.39217055160118264 2216 80-81 -0.3714432859762553 2216 82-83 -0.3754218334019228 2216 84-85 -0.4341121922388069 2216 86-87 -0.40050158960228543 2216 88-89 -0.4749892964840612 2216 90-91 -0.4229089202947094 2216 92-93 -0.4280796488464844 2216 94-95 -0.3570298241467782 2216 96-97 -0.41447164577971307 2216 98-99 -0.47566229943763716 2216 100 -0.5031400960853283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 5.0 4 7.0 5 29.0 6 84.0 7 182.0 8 383.0 9 811.0 10 1490.0 11 2354.0 12 3621.0 13 5853.0 14 7037.0 15 9157.0 16 11122.0 17 15633.0 18 18097.0 19 9655.0 20 6628.0 21 6448.0 22 6606.0 23 7233.0 24 8197.0 25 10104.0 26 12805.0 27 16731.0 28 21800.0 29 28044.0 30 36315.0 31 46738.0 32 59827.0 33 77759.0 34 106870.0 35 161316.0 36 290421.0 37 653397.0 38 1210757.0 39 600252.0 40 2182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75036525425372 19.85463358347496 13.53254345201238 23.862457710258933 2 15.594506402912128 20.138906211477384 40.38945368584398 23.877133699766507 3 18.338353805865417 25.186162593862583 30.026361097848575 26.449122502423428 4 12.18580307036657 16.700840366286503 39.02723305305691 32.086123510290015 5 12.426521757372418 37.76322319011677 36.05067195608739 13.75958309642342 6 30.849644758825473 38.39434577116299 17.4249311769053 13.331078293106232 7 26.987752187233433 32.58022942368613 22.520344540626002 17.91167384845444 8 24.943054830559184 36.322655728255796 20.252485853407855 18.48180358777716 9 24.90821663651589 16.398347899457285 21.46561711869684 37.227818345329986 10-11 23.823076016056376 26.607348635938482 28.969638572345215 20.59993677565993 12-13 24.774520220696743 23.945200239354346 29.26335459512888 22.016924944820026 14-15 21.75801844556068 25.907321959048353 26.75979291966747 25.574866675723502 16-17 20.93009561896974 29.4579228644805 27.24219763761494 22.36978387893482 18-19 20.70284581663508 27.93528552207063 30.09888742603336 21.262981235260927 20-21 21.90700537013851 27.263819086026093 29.70943265306949 21.119742890765902 22-23 21.424067820249025 27.106007333999045 29.859034786002958 21.61089005974897 24-25 21.32554151110077 27.19644865594828 29.838558698075857 21.639451134875088 26-27 21.114295465557394 27.135242759644605 29.946198732942324 21.80426304185567 28-29 21.160176639954813 27.20416185794005 29.98514304216503 21.65051845994011 30-31 21.376855416813033 27.748641333440588 29.482008193377123 21.392495056369253 32-33 21.08951366391548 27.355797108997663 30.033024070852587 21.521665156234267 34-35 21.166872687331235 27.127336974574966 30.04678289472923 21.659007443364573 36-37 21.565950918470215 27.60037430945461 29.348540667668992 21.485134104406182 38-39 21.44011048726202 27.708361545432762 29.424004416712673 21.427523550592543 40-41 21.254855017171778 27.464531573128287 29.60500711668911 21.67560629301083 42-43 21.317496362809337 27.321363879947825 29.448324693197037 21.912815064045805 44-45 21.486016636747372 27.636268780577577 29.207263154361563 21.670451428313488 46-47 21.59154435636482 27.919388049181066 28.887815382336157 21.60125221211795 48-49 21.586422775237242 27.749507807372993 28.732967125814966 21.931102291574796 50-51 21.668642960401208 27.731220579844003 28.581894950294622 22.018241509460168 52-53 21.662002266227308 27.82207800775702 28.53225612303832 21.983663602977348 54-55 21.74494583855619 28.15357740994987 28.38783910943517 21.71363764205877 56-57 21.515791252455177 27.878010303348066 28.522475928568703 22.083722515628057 58-59 21.36785857723055 27.49007223454874 28.909285513390763 22.232783674829946 60-61 21.69660910819673 27.93502767968648 28.441760388707753 21.926602823409038 62-63 21.472416958047017 27.840509912718996 28.562421367815173 22.12465176141881 64-65 21.537637544835537 27.544818175189324 28.701441913168075 22.21610236680706 66-67 21.37276623436561 27.668174157510634 28.57757322957838 22.381486378545375 68-69 21.89608496399551 27.830271664181105 28.400909386051566 21.872733985771816 70-71 21.823031406437018 27.66661164100836 28.384153941440644 22.126203011113976 72-73 21.651019761542035 27.765697180663523 28.536583954362833 22.046699103431614 74-75 21.678055693963874 27.81476221643897 28.35164663459452 22.155535455002635 76-77 21.654030539669975 27.810185522765863 28.24039833748268 22.29538560008148 78-79 21.86809526951091 27.92304838823553 28.26599177537729 21.942864566876274 80-81 21.6993290439368 27.713425255587154 28.405894853071384 22.18135084740466 82-83 21.50408280188428 27.850134260813203 28.499186911885555 22.14659602541696 84-85 21.544770142281575 27.685415704561738 28.40436127228177 22.365452880874916 86-87 21.6319070542243 27.845928348800136 28.260676766059007 22.26148783091656 88-89 21.415635976261434 27.695730383051227 28.331137327503104 22.557496313184238 90-91 21.380998513266306 27.629909794791395 28.40909945837522 22.57999223356708 92-93 21.504553388836765 27.840191206884953 28.229896764775454 22.425358639502825 94-95 21.253413681811 27.54710287718616 28.387393784509364 22.812089656493477 96-97 21.253022499855685 27.480287289578815 28.439472093897045 22.82721811666846 98-99 21.241079538269986 27.60249759907453 28.326536859956963 22.829886002698526 100 21.359973657133004 27.485095330854143 28.177608331568273 22.977322680444587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19.0 1 23.5 2 72.5 3 135.5 4 105.0 5 78.0 6 196.5 7 456.0 8 836.5 9 1037.5 10 918.0 11 833.5 12 936.5 13 1169.5 14 1394.5 15 1620.5 16 1909.5 17 2245.0 18 2713.5 19 3242.0 20 3949.0 21 4903.5 22 6370.0 23 8759.0 24 11952.0 25 15570.5 26 20767.0 27 27758.0 28 35857.5 29 44940.5 30 54440.0 31 64830.0 32 75512.5 33 85758.5 34 96147.5 35 105336.5 36 112780.0 37 119852.5 38 124890.0 39 126438.0 40 127857.0 41 129084.5 42 128269.0 43 127071.5 44 124200.0 45 121312.5 46 119472.5 47 115734.5 48 112085.0 49 109852.0 50 106686.5 51 101579.5 52 94218.5 53 88646.0 54 84891.5 55 80008.5 56 74139.5 57 68085.5 58 62359.0 59 56788.5 60 49919.0 61 41837.0 62 35295.5 63 29459.5 64 24167.0 65 19325.0 66 14882.0 67 11107.0 68 8410.5 69 6620.5 70 4825.5 71 3360.5 72 2377.0 73 1718.0 74 1280.0 75 850.5 76 547.5 77 337.0 78 223.5 79 151.0 80 85.0 81 48.0 82 27.0 83 12.0 84 2.0 85 1.5 86 1.0 87 1.5 88 2.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04401087511328243 2 0.2405696355633334 3 0.0032986454720014442 4 0.002604193793685351 5 2.604193793685351E-4 6 5.7870973193007795E-5 7 0.0 8 2.3148389277203118E-4 9 0.0 10-11 4.774355288423143E-4 12-13 0.0 14-15 3.0382260926329095E-4 16-17 2.1701614947377922E-4 18-19 4.050968123510546E-4 20-21 2.8935486596503898E-5 22-23 1.446774329825195E-4 24-25 9.548710576846286E-4 26-27 1.3020968968426754E-4 28-29 2.1701614947377922E-4 30-31 5.7870973193007795E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.68064597895117E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4467743298251949E-5 68-69 1.736129195790234E-4 70-71 1.4467743298251949E-5 72-73 2.604193793685351E-4 74-75 1.736129195790234E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1574194638601559E-4 84-85 0.0 86-87 3.906290690528026E-4 88-89 0.0019820808318605173 90-91 0.0019097421153692572 92-93 6.221129618248338E-4 94-95 5.208387587370702E-4 96-97 2.4595163607028315E-4 98-99 1.446774329825195E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3455964.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.13460050422182 #Duplication Level Percentage of deduplicated Percentage of total 1 73.53948980203647 44.22267840529758 2 15.426892021756508 18.55379977500189 3 5.078076043197118 9.161042225631546 4 2.239390135661817 5.386593247244739 5 1.1603043008145397 3.4887217796406382 6 0.6543049980515991 2.360782179944913 7 0.42520310929358 1.7898593377365684 8 0.2991153524116919 1.4389745777565293 9 0.21054469284664365 1.1394918893355306 >10 0.9157007847490221 9.282031042593838 >50 0.03957948347807185 1.621352435551435 >100 0.010822024159259508 1.025476611977599 >500 3.84834365910463E-4 0.13971898445295688 >1k 1.4431288338366256E-4 0.23387742504750583 >5k 4.810429446122086E-5 0.15560008278670798 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5379 0.15564398240259447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4544 0.13148285109451371 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.68064597895117E-5 0.0 4 0.0 0.0 0.0 8.68064597895117E-5 0.0 5 0.0 0.0 0.0 1.1574194638601559E-4 0.0 6 0.0 0.0 0.0 1.1574194638601559E-4 0.0 7 0.0 0.0 0.0 1.1574194638601559E-4 0.0 8 0.0 0.0 0.0 1.1574194638601559E-4 0.0 9 0.0 0.0 0.0 1.736129195790234E-4 0.0 10-11 0.0 1.4467743298251949E-5 0.0 2.0254840617552728E-4 0.0 12-13 0.0 2.8935486596503898E-5 0.0 2.3148389277203118E-4 0.0 14-15 0.0 2.8935486596503898E-5 0.0 3.0382260926329095E-4 0.0 16-17 0.0 2.8935486596503898E-5 0.0 3.761613257545507E-4 0.0 18-19 0.0 2.8935486596503898E-5 0.0 3.761613257545507E-4 0.0 20-21 0.0 2.8935486596503898E-5 0.0 4.195645556493065E-4 0.0 22-23 0.0 2.8935486596503898E-5 0.0 5.49774245333574E-4 0.0 24-25 0.0 2.8935486596503898E-5 0.0 6.365807051230859E-4 0.0 26-27 0.0 2.8935486596503898E-5 0.0 7.957258814038572E-4 0.0 28-29 0.0 2.8935486596503898E-5 0.0 0.0014612420731234467 0.0 30-31 0.0 2.8935486596503898E-5 0.0 0.0036314035678612394 0.0 32-33 0.0 2.8935486596503898E-5 0.0 0.00943296863046027 0.0 34-35 0.0 2.8935486596503898E-5 0.0 0.016883856429060026 0.0 36-37 0.0 2.8935486596503898E-5 0.0 0.030917567428364413 0.0 38-39 0.0 2.8935486596503898E-5 0.0 0.055165505196234685 0.0 40-41 0.0 2.8935486596503898E-5 0.0 0.08693666947919595 0.0 42-43 0.0 2.8935486596503898E-5 0.0 0.12142776950222861 0.0 44-45 0.0 2.8935486596503898E-5 0.0 0.15797328907361302 0.0 46-47 0.0 2.8935486596503898E-5 0.0 0.2033875352868259 0.0 48-49 0.0 5.7870973193007795E-5 0.0 0.25934876636446447 0.0 50-51 0.0 5.7870973193007795E-5 0.0 0.32020009467691213 0.0 52-53 0.0 5.7870973193007795E-5 0.0 0.3809790842728686 0.0 54-55 0.0 5.7870973193007795E-5 0.0 0.44748730021493277 0.0 56-57 0.0 5.7870973193007795E-5 0.0 0.5284053884820559 0.0 58-59 0.0 5.7870973193007795E-5 0.0 0.6190023970157097 0.0 60-61 0.0 5.7870973193007795E-5 0.0 0.7134767607532949 0.0 62-63 0.0 5.7870973193007795E-5 0.0 0.8138828992431635 0.0 64-65 0.0 5.7870973193007795E-5 0.0 0.9249662322871419 0.0 66-67 0.0 5.7870973193007795E-5 0.0 1.051645792606636 0.0 68-69 0.0 5.7870973193007795E-5 0.0 1.1984499838539984 0.0 70-71 0.0 5.7870973193007795E-5 0.0 1.3581015311502087 0.0 72-73 0.0 5.7870973193007795E-5 0.0 1.5299638537901437 0.0 74-75 0.0 5.7870973193007795E-5 0.0 1.7053852412814487 0.0 76-77 0.0 5.7870973193007795E-5 0.0 1.8939722751741628 0.0 78-79 0.0 5.7870973193007795E-5 0.0 2.112362860261276 0.0 80-81 0.0 5.7870973193007795E-5 0.0 2.3523537860926793 0.0 82-83 0.0 5.7870973193007795E-5 0.0 2.6009674869298407 0.0 84-85 0.0 5.7870973193007795E-5 0.0 2.8555129625192857 0.0 86-87 0.0 8.68064597895117E-5 0.0 3.1250325524224207 0.0 88 0.0 8.68064597895117E-5 0.0 3.3502663800896073 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4975 0.0 50.59079 1 GTATCAA 8825 0.0 40.69711 1 TCAACGC 10295 0.0 34.467064 4 ATCAACG 10305 0.0 34.34389 3 CAACGCA 10610 0.0 33.354214 5 AACGCAG 10905 0.0 32.495018 6 TATCAAC 11260 0.0 31.789488 2 ACGCAGA 12555 0.0 28.037302 7 CGCAGAG 12660 0.0 27.73052 8 GTACATG 8560 0.0 27.200476 1 GTGGTAT 2365 0.0 25.708803 1 TACATGG 8745 0.0 25.690166 2 TCGTACG 95 0.0012892665 24.788755 2 GCAGAGT 14335 0.0 24.293587 9 ACATGGG 9305 0.0 23.033026 3 TGGTATC 2650 0.0 21.327682 2 GAGTACT 10190 0.0 21.262156 12-13 CAGAGTA 14385 0.0 20.255966 10-11 AGAGTAC 13180 0.0 20.253683 10-11 GAGTACA 6315 0.0 20.00252 1 >>END_MODULE