FastQCFastQC Report
Wed 25 May 2016
SRR1033093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96366
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20672.1449473880829335No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3260.33829358902517487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2090.2168814727185937No Hit
GTACGGGAGAGACGTGAGTTCCTGGGTGGGGCACTATGGCGCCCTTGTGA1950.20235352717763527No Hit
CTCCCGTACTGCTCAAAAGATACGTAGCGGATGCCCTTGCCAAAGTTGGT1740.18056160886619763No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.16914679451258743No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1490.154618848971629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.13490235145175686No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1120.11622356432766742No Hit
GTACATGCATCGAGAGGACTAATGGAGCGCTTAGAAGTGAGAATGCCGGT1080.11207272274453646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.1110350123487537No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1050.10895959155718822No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1030.10688417076562273No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.1037710395782745No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA200.002156703370.5019451
CAGAACA250.005220930656.4015584
GCCGGAA250.005220930656.40155894
TCTAGAG250.005220930656.4015582
TACATGA451.6025559E-552.2236672
AGTACAA555.2460622E-542.7284552
ACATGAA704.5901397E-640.2868273
GGAAACA1800.039.167751
GAGAACA1800.036.556571
AGAACAA1551.8189894E-1236.38812
CATGGAA550.002704334434.1827662
GTACAAA1404.984031E-1033.5723571
CATGAAA753.2529148E-431.33424
ATCAACA2400.031.33422
GTCAAGA600.004141659531.33421
TGGTACA300.004151888731.334258-59
TAGACAG953.7163263E-529.685035
CCCCTTG404.7654918E-429.3758148-49
TCAACAC2250.029.2452553
ACAACAC2600.028.9238765
Produced by FastQC (version 0.11.5)