##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033093_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96366
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.006963036755703	31.0	27.0	34.0	12.0	34.0
2	27.93256957848204	31.0	27.0	34.0	10.0	34.0
3	28.047994105804953	31.0	27.0	34.0	10.0	34.0
4	30.770209409957868	37.0	31.0	37.0	7.0	37.0
5	31.45333416350165	37.0	32.0	37.0	10.0	37.0
6	31.068032293547517	37.0	32.0	37.0	8.0	37.0
7	31.34108502998983	37.0	32.0	37.0	8.0	37.0
8	30.79433617665982	37.0	31.0	37.0	8.0	37.0
9	31.830313596081606	39.0	31.0	39.0	8.0	39.0
10-11	32.260973787435404	39.0	31.5	39.0	7.5	39.0
12-13	32.27736961168877	39.0	32.0	39.0	7.0	39.0
14-15	33.403441047672416	40.0	32.0	41.0	7.0	41.0
16-17	33.64127389328186	40.0	32.0	41.0	8.0	41.0
18-19	33.66877840732209	40.0	32.0	41.0	8.0	41.0
20-21	33.70583504555549	40.0	32.0	41.0	8.0	41.0
22-23	33.64803457651038	40.0	31.5	41.0	8.0	41.0
24-25	33.565313492310565	40.0	31.0	41.0	5.0	41.0
26-27	33.096008965817816	39.0	30.5	41.0	2.0	41.0
28-29	32.73731917896353	39.0	30.0	41.0	2.0	41.0
30-31	32.42764564265404	40.0	30.0	41.0	2.0	41.0
32-33	32.018756615403774	40.0	30.0	41.0	2.0	41.0
34-35	31.61972583691343	40.0	30.0	41.0	2.0	41.0
36-37	31.2527810638607	39.5	30.0	41.0	2.0	41.0
38-39	30.89354129049665	39.0	29.0	41.0	2.0	41.0
40-41	30.646535084988482	39.0	28.5	41.0	2.0	41.0
42-43	30.371028163460142	39.0	27.0	41.0	2.0	41.0
44-45	30.140895128987403	39.0	27.0	41.0	2.0	41.0
46-47	29.899772741423323	38.0	26.0	41.0	2.0	41.0
48-49	29.671258535168008	38.0	26.0	41.0	2.0	41.0
50-51	29.41006682854949	38.0	25.0	41.0	2.0	41.0
52-53	29.19913143639873	37.0	24.0	40.5	2.0	41.0
54-55	28.96913330427744	37.0	23.0	40.0	2.0	41.0
56-57	28.7190450988938	36.5	22.5	40.0	2.0	41.0
58-59	28.448877197351763	36.0	20.5	40.0	2.0	41.0
60-61	28.115943382520808	35.0	16.5	39.5	2.0	41.0
62-63	27.797599775854557	35.0	10.0	39.0	2.0	41.0
64-65	27.485897515721312	35.0	4.5	39.0	2.0	41.0
66-67	27.169624141294648	35.0	2.0	38.0	2.0	40.5
68-69	26.802368055123175	35.0	2.0	37.0	2.0	40.0
70-71	26.448591826992924	35.0	2.0	36.5	2.0	39.5
72-73	26.147396384616982	35.0	2.0	36.0	2.0	39.0
74-75	25.868900857148788	34.0	2.0	35.5	2.0	39.0
76-77	25.590976070398273	34.0	2.0	35.0	2.0	37.0
78-79	25.29798372870099	34.0	2.0	35.0	2.0	37.0
80-81	25.051942593860907	34.0	2.0	35.0	2.0	36.5
82-83	24.949816325259945	34.0	2.0	35.0	2.0	36.0
84-85	24.845407093788268	34.0	2.0	35.0	2.0	36.0
86-87	24.7831444700413	34.0	2.0	35.0	2.0	35.5
88-89	24.645575202872383	34.0	2.0	35.0	2.0	35.0
90-91	24.474415250191974	34.0	2.0	35.0	2.0	35.0
92-93	24.264143992694517	34.0	2.0	35.0	2.0	35.0
94-95	24.096854699790384	34.0	2.0	35.0	2.0	35.0
96-97	23.936772305584956	34.0	2.0	35.0	2.0	35.0
98-99	23.76442417450138	33.5	2.0	35.0	2.0	35.0
100	23.629807193408464	33.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.520040660095276
1101	2	1.3851067583201484
1101	3	1.399968257069549
1101	4	2.0973376409591467
1101	5	1.5056734787775312
1101	6	1.871014747600963
1101	7	2.0473214939079547
1101	8	2.65832555514465
1101	9	2.096248806448351
1101	10-11	2.2955834275692695
1101	12-13	2.280241087008619
1101	14-15	2.547043090548911
1101	16-17	2.6558460397255743
1101	18-19	2.4750809815613124
1101	20-21	2.3754818067322248
1101	22-23	2.322513615709866
1101	24-25	2.1443964498114525
1101	26-27	2.7748638480818073
1101	28-29	2.648394862167308
1101	30-31	2.8938816010180233
1101	32-33	2.994298252393264
1101	34-35	3.2311741956335567
1101	36-37	3.140172774971866
1101	38-39	2.5730688999553166
1101	40-41	2.7157888368376852
1101	42-43	2.529217245327388
1101	44-45	2.732053798906122
1101	46-47	2.8131391448806795
1101	48-49	2.579747991087391
1101	50-51	2.4680593824659773
1101	52-53	2.5787411892600893
1101	54-55	2.45593982174184
1101	56-57	2.5093567488021478
1101	58-59	2.778037682883518
1101	60-61	2.5775915851708966
1101	62-63	2.4024085015002754
1101	64-65	2.704722769152472
1101	66-67	2.5365165436691655
1101	68-69	2.683108425689774
1101	70-71	2.599576216343955
1101	72-73	2.409520264223115
1101	74-75	2.269078679156504
1101	76-77	2.5645643925966866
1101	78-79	2.4856181350060638
1101	80-81	2.573878701238158
1101	82-83	2.790008973668119
1101	84-85	2.535311785607753
1101	86-87	2.622548673707957
1101	88-89	2.4291205112655945
1101	90-91	2.576676227584745
1101	92-93	2.5031587658769503
1101	94-95	2.4606640509606628
1101	96-97	2.461694793501536
1101	98-99	2.1729715489129617
1101	100	2.0917124565423144
1102	1	-0.6947681822080227
1102	2	-0.6060240620789621
1102	3	-0.3651328650160508
1102	4	-0.9185193771151674
1102	5	-0.6658645175941693
1102	6	-1.6577092999700938
1102	7	-0.9339322855719878
1102	8	-1.1223638219970589
1102	9	-0.8397855952179789
1102	10-11	-0.959002341428917
1102	12-13	-0.2622906606229094
1102	14-15	-1.1741488734368417
1102	16-17	-0.9125877437858207
1102	18-19	-0.6880917658686414
1102	20-21	-0.6469263099109241
1102	22-23	-0.7556628272115802
1102	24-25	-0.2667437772932999
1102	26-27	0.067748347174728
1102	28-29	-0.24292409234455192
1102	30-31	0.4106120989015096
1102	32-33	0.29121419675932003
1102	34-35	0.2801562557020816
1102	36-37	0.0789284021975547
1102	38-39	0.32375760518947416
1102	40-41	0.5532544015759697
1102	42-43	0.22433919654690015
1102	44-45	-0.4182183236498105
1102	46-47	-0.44053954893105285
1102	48-49	-0.10596725449481781
1102	50-51	-0.2803784324906822
1102	52-53	-0.3957935147646374
1102	54-55	-1.3914027839156198
1102	56-57	-1.20528411661477
1102	58-59	-1.135319839121454
1102	60-61	-1.529476882207991
1102	62-63	-1.794964339996735
1102	64-65	-1.3990197558760862
1102	66-67	-1.3642427118572762
1102	68-69	-1.4246655446119085
1102	70-71	-1.476618099318209
1102	72-73	-1.36139285813125
1102	74-75	-1.2387490437488253
1102	76-77	-1.5161652989977519
1102	78-79	-1.3622284013105386
1102	80-81	-1.3119306114004203
1102	82-83	-1.0904250786062875
1102	84-85	-1.0001985281730725
1102	86-87	-0.7182428333806676
1102	88-89	-0.7680171625904819
1102	90-91	-0.8601644631362113
1102	92-93	-0.8756627082882034
1102	94-95	-0.7567316353928959
1102	96-97	-0.42021745537302024
1102	98-99	-0.7384038458327211
1102	100	-1.2625172014567454
1103	1	-0.07403647489094922
1103	2	0.188894637108028
1103	3	0.2962492488050863
1103	4	0.19713102938889548
1103	5	-0.9022466314153093
1103	6	0.13781915531446032
1103	7	-0.5331192774419087
1103	8	-0.03740447240356204
1103	9	0.6051331039690169
1103	10-11	0.23439196751417057
1103	12-13	0.09370527433643616
1103	14-15	-0.11459602790838375
1103	16-17	0.35966835377515594
1103	18-19	0.4607903479524893
1103	20-21	0.20988263317850198
1103	22-23	0.06466237604044522
1103	24-25	0.9217724828693008
1103	26-27	0.9372605422966771
1103	28-29	0.528112493021311
1103	30-31	0.5978072208527294
1103	32-33	0.5879621642389878
1103	34-35	0.8519042231817622
1103	36-37	1.1778105160186954
1103	38-39	0.673757605189472
1103	40-41	0.6615877349093076
1103	42-43	0.6417172453273885
1103	44-45	1.0278182617160425
1103	46-47	1.0993994754591903
1103	48-49	0.8540937211149391
1103	50-51	1.1058004292979327
1103	52-53	0.6115235584060947
1103	54-55	0.977702907141289
1103	56-57	1.2506101923283204
1103	58-59	1.3357167462443975
1103	60-61	1.1595475080359101
1103	62-63	1.0014787494341597
1103	64-65	1.02872414656294
1103	66-67	0.9648223287931295
1103	68-69	1.3383425854693876
1103	70-71	1.45945913645415
1103	72-73	1.4358632394297253
1103	74-75	1.1168403871454835
1103	76-77	1.5663550262055033
1103	78-79	1.8222228182016522
1103	80-81	1.609105973965434
1103	82-83	1.5974814254587528
1103	84-85	1.6847608214204222
1103	86-87	1.7597043210908758
1103	88-89	1.655153569116834
1103	90-91	1.952640414912569
1103	92-93	1.9348047713865952
1103	94-95	1.4616626735501903
1103	96-97	1.278258154383078
1103	98-99	1.5442181053867898
1103	100	1.731588489600167
1104	1	0.06511081193075441
1104	2	-0.37951621560515036
1104	3	-0.02971974344297479
1104	4	0.005851959621455904
1104	5	-0.9547660112602685
1104	6	-0.12225836406538804
1104	7	0.17308227294568823
1104	8	0.37073506248015775
1104	9	0.6115284528062261
1104	10-11	0.11917878921960323
1104	12-13	-0.0370117799271199
1104	14-15	-0.13765804341225873
1104	16-17	0.3715869584263203
1104	18-19	0.4845306580300175
1104	20-21	0.7861423231009823
1104	22-23	0.8693135388311504
1104	24-25	0.6422182193034089
1104	26-27	0.9810589919090802
1104	28-29	1.1520466015484345
1104	30-31	1.0119545076744387
1104	32-33	1.130597823153721
1104	34-35	0.7939584867476555
1104	36-37	0.3717058648559046
1104	38-39	0.7617421013135086
1104	40-41	1.1927892852969002
1104	42-43	1.1261164701335886
1104	44-45	0.8643492694679793
1104	46-47	0.9967831963894227
1104	48-49	1.1195006978591273
1104	50-51	1.2647151579801061
1104	52-53	0.9355545661580287
1104	54-55	0.549795930397103
1104	56-57	0.7721218202352986
1104	58-59	1.0154648082599032
1104	60-61	0.40567153904366293
1104	62-63	0.5643663463333866
1104	64-65	0.44858848764821246
1104	66-67	0.4847835691032074
1104	68-69	0.08601700407404067
1104	70-71	0.2198273535084141
1104	72-73	0.2987508363289493
1104	74-75	0.5221698445098255
1104	76-77	0.6248821579884485
1104	78-79	0.6511956864187098
1104	80-81	0.5559082995468287
1104	82-83	0.5328496425130176
1104	84-85	0.44638872839716726
1104	86-87	0.33441362341645586
1104	88-89	0.5687194605896977
1104	90-91	0.24004351568776272
1104	92-93	-0.0579277867529413
1104	94-95	0.35633321618585256
1104	96-97	-0.018156574299094785
1104	98-99	-0.0742896465511933
1104	100	0.09728616401877233
1105	1	-0.504571754696304
1105	2	-0.29571606848807974
1105	3	-0.08805732053797755
1105	4	-0.31582517499066753
1105	5	0.16411589899831824
1105	6	0.19025224533879026
1105	7	0.3790461726797467
1105	8	-0.05115143104103126
1105	9	0.18341777550185867
1105	10-11	0.20060242980127896
1105	12-13	-0.017943144155040613
1105	14-15	0.6554161375417351
1105	16-17	0.15702236837369554
1105	18-19	-0.14215977370200505
1105	20-21	-0.05918670988719299
1105	22-23	-0.6988412735945886
1105	24-25	-1.5677348164007725
1105	26-27	-1.147046027046386
1105	28-29	-1.0819544169543676
1105	30-31	-1.0078497134538367
1105	32-33	-0.9278529209191575
1105	34-35	-0.8622685262099665
1105	36-37	-0.6099327929837415
1105	38-39	-0.6263640493117393
1105	40-41	-0.3786190777427656
1105	42-43	-0.3649433386142178
1105	44-45	-0.4604116652912609
1105	46-47	-0.3081735172415385
1105	48-49	-0.24684910126948623
1105	50-51	-0.097362587733695
1105	52-53	-0.11061756081531726
1105	54-55	-0.3709588933453283
1105	56-57	0.16113330668355275
1105	58-59	-0.22739152626168035
1105	60-61	0.05309981947143427
1105	62-63	0.07602254505459882
1105	64-65	-0.003909673388395873
1105	66-67	0.19821648937707081
1105	68-69	0.24114063899736848
1105	70-71	0.3990272629748297
1105	72-73	0.5512829474589225
1105	74-75	0.5705204358072855
1105	76-77	0.4555277755972291
1105	78-79	0.6842970274473998
1105	80-81	0.40500013454937545
1105	82-83	0.404263663901574
1105	84-85	0.4663909917367235
1105	86-87	0.4886885060057189
1105	88-89	0.4401900654671991
1105	90-91	0.41717812780794716
1105	92-93	0.3516235061797808
1105	94-95	0.35907751539934196
1105	96-97	0.3792009767675033
1105	98-99	0.4409030202286388
1105	100	0.5766614823008958
1106	1	0.3766727449672054
1106	2	0.5475825803704382
1106	3	0.71149747575544
1106	4	0.9873792563392492
1106	5	0.9312640068825644
1106	6	0.4147695099243869
1106	7	0.6931218573098654
1106	8	1.3019572297240956
1106	9	0.8386437422668891
1106	10-11	1.0995515419822581
1106	12-13	0.9553187495137436
1106	14-15	0.9472834047157264
1106	16-17	0.2787286374631037
1106	18-19	0.4310917663922069
1106	20-21	1.0984464629657396
1106	22-23	1.1901942909340661
1106	24-25	1.4692015608834836
1106	26-27	1.0957711805945536
1106	28-29	1.500718876000029
1106	30-31	1.7845093485123087
1106	32-33	1.559593369912747
1106	34-35	1.403145357933532
1106	36-37	1.6378282465151486
1106	38-39	1.4833320732745818
1106	40-41	1.5319778058312927
1106	42-43	1.8341817843344757
1106	44-45	1.69776507022668
1106	46-47	1.465445574749971
1106	48-49	1.5483313097674234
1106	50-51	1.6554458193688575
1106	52-53	1.8398923527323348
1106	54-55	1.5429511340916449
1106	56-57	1.5766740221155544
1106	58-59	1.6225075263862472
1106	60-61	1.6577744583905236
1106	62-63	1.595539033122101
1106	64-65	1.565692231669324
1106	66-67	1.7603897046796533
1106	68-69	1.5940163443346371
1106	70-71	1.7930584272342927
1106	72-73	1.8051008280822067
1106	74-75	1.7717162736703074
1106	76-77	1.3780571538650754
1106	78-79	1.1817086338044902
1106	80-81	1.7012159030434475
1106	82-83	1.5209743332601704
1106	84-85	1.7929168497892185
1106	86-87	1.3801830444951335
1106	88-89	1.670401796067189
1106	90-91	1.3865056631395198
1106	92-93	1.7091842040107075
1106	94-95	1.4330279217771427
1106	96-97	1.5308290763688923
1106	98-99	1.5382783890747334
1106	100	1.3633260782526477
1107	1	-0.7934428675850143
1107	2	-0.4940962304718788
1107	3	-0.64690143612642
1107	4	-0.5628004774604172
1107	5	-0.954872202191563
1107	6	0.10106116444687885
1107	7	-0.17649827287569764
1107	8	-0.47735881030310523
1107	9	-0.14794908781180638
1107	10-11	-0.05664684527121722
1107	12-13	-0.016968241645294313
1107	14-15	0.05675100405508715
1107	16-17	0.28620944966556294
1107	18-19	0.11803920639998466
1107	20-21	-0.2526173668214966
1107	22-23	-0.7113650212198266
1107	24-25	-1.082508339048509
1107	26-27	-1.0894517864704483
1107	28-29	-1.6779948129147684
1107	30-31	-1.447055792845898
1107	32-33	-1.3755081553957105
1107	34-35	-1.3488948635762306
1107	36-37	-1.4756712191411232
1107	38-39	-1.324644221294541
1107	40-41	-0.9958323564148976
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2208	84-85	-0.21195279360305364
2208	86-87	0.10049070606740074
2208	88-89	-0.33569150130570335
2208	90-91	-0.3840966742893066
2208	92-93	-0.37886893753359274
2208	94-95	-0.10987488513525179
2208	96-97	-0.4554859770723212
2208	98-99	-0.49985466456626426
2208	100	-0.9046791160336376
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2209	2	0.43387256205284075
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2209	5	0.8666827284080938
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2210	4	-1.317868970611105
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2210	8	-1.3874044724035635
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2210	12-13	-0.8554613923302234
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2212	4	-0.7885832563253885
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2213	8	-1.0517455576748809
2213	9	-2.115215733240287
2213	10-11	-1.5881855518656707
2213	12-13	-1.6494148807023166
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2213	32-33	-3.038975820257132
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2213	54-55	-2.0190025192153023
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2213	58-59	-2.335310385538545
2213	60-61	-2.262933112119125
2213	62-63	-1.9627654366123508
2213	64-65	-2.064977403824656
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2213	78-79	-1.6220601275347803
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2213	98-99	-2.114987320969803
2213	100	-2.1817836034230886
2214	1	-0.667975868830343
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2214	4	-0.6960507887929204
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2214	84-85	-0.7650497846401834
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2215	1	0.07016642365977432
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2215	4	-0.30178201408936545
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2215	9	0.03556788657770937
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2215	12-13	-0.35720052276500525
2215	14-15	-0.325284433705491
2215	16-17	-0.07728816796397098
2215	18-19	-0.3725429853808393
2215	20-21	-0.3748999755171525
2215	22-23	-0.2299028413508566
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2215	84-85	0.5497970533044807
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2215	90-91	0.9229302699850344
2215	92-93	1.0072685395025367
2215	94-95	0.647894557608165
2215	96-97	0.8327871398903248
2215	98-99	1.136971728575194
2215	100	1.1978928374262523
2216	1	0.4246814738270004
2216	2	0.6587664319798243
2216	3	0.13342873598457317
2216	4	0.5016182088760743
2216	5	0.06634311217443667
2216	6	0.08012684762215017
2216	7	0.35662431230168323
2216	8	0.4433647583656679
2216	9	0.38397925781517017
2216	10-11	0.13855863418083914
2216	12-13	0.27479501792618777
2216	14-15	0.1591219208095609
2216	16-17	-0.1848829282761244
2216	18-19	-0.12286349820134745
2216	20-21	0.44738263317850624
2216	22-23	-0.14815813678006862
2216	24-25	-0.16124033764351964
2216	26-27	-0.09991894488280195
2216	28-29	0.22843300584182202
2216	30-31	0.4407559388014519
2216	32-33	0.546936523213347
2216	34-35	0.6204939667715053
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2216	40-41	0.1917159400375077
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2216	50-51	0.3160568395543457
2216	52-53	-0.11668156979903799
2216	54-55	0.18283111226949345
2216	56-57	0.7384307051488364
2216	58-59	0.3950116180392733
2216	60-61	0.6198039182923232
2216	62-63	0.5550043904597999
2216	64-65	0.2809677363065326
2216	66-67	0.5577710467418484
2216	68-69	0.6165477136745174
2216	70-71	0.818754008249023
2216	72-73	0.7688760599425457
2216	74-75	0.7684429512480513
2216	76-77	0.6368678467183209
2216	78-79	0.6186971771760135
2216	80-81	0.6337854611449174
2216	82-83	0.5420327075100388
2216	84-85	0.6552736419332454
2216	86-87	0.4766915005780561
2216	88-89	0.6820766460399099
2216	90-91	0.6302045174766704
2216	92-93	0.5851252842071055
2216	94-95	0.48441908380660337
2216	96-97	0.512553026177951
2216	98-99	0.34133349000217095
2216	100	0.23479361780529473
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5686.0
3	765.0
4	471.0
5	540.0
6	1572.0
7	3205.0
8	3438.0
9	1985.0
10	1115.0
11	1108.0
12	1117.0
13	694.0
14	567.0
15	423.0
16	455.0
17	444.0
18	383.0
19	342.0
20	286.0
21	268.0
22	341.0
23	639.0
24	996.0
25	810.0
26	730.0
27	634.0
28	759.0
29	840.0
30	996.0
31	1215.0
32	1618.0
33	2184.0
34	3144.0
35	5775.0
36	9871.0
37	19422.0
38	17418.0
39	4103.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.44678641885578	19.909666701277125	12.578133111826395	20.065413768040703
2	17.33009406341494	26.75512365289977	32.715587948254736	23.199194335430555
3	21.305602857498858	31.37641732774017	24.6376209660672	22.680358848693775
4	13.8191711404866	23.420317546027558	29.07238761798943	33.688123695496415
5	13.493753180529447	44.821319153797425	26.154597098318604	15.530330567354527
6	29.085225266393866	39.425253930997236	14.08304426396361	17.406476538645286
7	25.46374192476268	41.73054153424316	15.444215948983194	17.361500592010966
8	22.0934218316662	41.26909933210765	15.953590310886751	20.6838885253394
9	22.429498831472344	28.40820566086731	15.320695923136848	33.8415995845235
10-11	21.724859646116055	36.48058416299228	20.406753605575723	21.387802585315946
12-13	22.49680575898283	36.37695160335318	19.98659976939138	21.139642868272617
14-15	21.67129761873848	36.857358020203066	19.240177620815913	22.231166740242543
16-17	20.336562925117086	38.67929426669574	20.341755194865886	20.642387613321286
18-19	20.87176451036603	37.494806597698286	21.8247538327309	19.808675059204788
20-21	23.018918876811785	34.99239194220992	21.427718257780732	20.560970923197566
22-23	22.202897948800484	35.075979005186404	21.46984461553637	21.251278430476745
24-25	23.315653446539965	35.69330413211131	20.379788879316607	20.611253542032117
26-27	22.071058498282518	34.87759375990605	21.80291322174471	21.248434520066724
28-29	21.83976076256931	35.53101572072352	22.25614188108737	20.373081635619794
30-31	21.980777917971245	36.630475982782166	21.228406311821423	20.160339787425166
32-33	21.08949497152173	36.98748202260609	21.94202582514291	19.980997180729272
34-35	20.35613637189315	36.54264346399971	21.559692545632892	21.541527618474248
36-37	20.69631490961197	36.51188387136968	21.88054866209102	20.911252556927327
38-39	20.87100107486123	35.403955697024145	22.60947227948469	21.11557094862994
40-41	19.63503990777332	36.07849653890294	22.982930793637603	21.30353275968614
42-43	19.40720123793995	36.49378433673628	22.70041230047046	21.398602124853305
44-45	20.201679275948553	35.52546174896019	23.19052045092246	21.0823385241688
46-47	19.55134363235103	35.47449045826301	22.956250811372193	22.01791509801376
48-49	19.93571302460353	34.040420825241206	22.546657388847922	23.47720876130734
50-51	19.426847505504092	33.32935238649109	22.621297719436715	24.622502388568105
52-53	20.069270633197977	32.6511854936701	22.01964918111103	25.259894692020897
54-55	20.401360367610792	32.122848464394195	22.1942418027467	25.281549365248317
56-57	21.0797956301404	31.804540167815905	21.742336130265016	25.373328071778683
58-59	21.24272429423707	31.290337655054962	21.781165462919212	25.685772587788758
60-61	22.031900642789644	30.682042388809855	21.530857018245257	25.755199950155244
62-63	22.086762377008746	30.590098393000858	21.804823593551234	25.51831563643916
64-65	21.95135881699326	30.48225801426836	22.267568044695057	25.298815124043323
66-67	23.229176402172445	30.179029460938555	21.8501095568917	24.7416845799973
68-69	23.88377442351927	30.820235628871544	21.97818174265672	23.317808204952463
70-71	24.088553835290696	30.788760474751562	21.527885943324716	23.594799746633022
72-73	24.616319156005982	29.78069446751952	22.717644126931383	22.885342249543115
74-75	24.855800430911405	29.18931547387275	23.02883991381772	22.926044181398126
76-77	25.116423954520677	28.57461776081821	23.343976325831324	22.96498195882979
78-79	26.71425604817776	27.445228948188145	23.61229363513654	22.22822136849756
80-81	26.670958163101087	27.087821667557844	23.960566685182396	22.28065348415867
82-83	26.707784319222505	26.207311881547934	24.797786292039163	22.2871175071904
84-85	27.06461308457789	26.809213087692523	23.907412310072207	22.218761517657377
86-87	26.917286988299537	27.029931167658145	23.41649276897042	22.636289075071893
88-89	26.483489672285177	26.605480774721368	23.870803635853964	23.04022591713949
90-91	26.8167212587144	26.8930290021335	23.563519707642712	22.726730031509387
92-93	26.64012292486425	27.219966984707067	23.78450772952377	22.35540236090491
94-95	25.496516855099095	27.991819021812482	23.73573779341992	22.775926329668504
96-97	24.834921821466395	28.981602608027572	23.231379389106916	22.952096181399114
98-99	23.269793808010963	30.636018189746466	22.746527128885567	23.347660873357004
100	22.953764367517728	31.054604354642773	22.488604624601553	23.50302665323795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1402.0
1	841.0
2	268.5
3	231.5
4	178.5
5	129.0
6	101.0
7	114.5
8	145.0
9	153.0
10	114.0
11	65.5
12	58.5
13	59.5
14	55.0
15	59.5
16	63.5
17	71.0
18	86.5
19	95.0
20	122.0
21	171.0
22	244.0
23	319.5
24	392.5
25	471.0
26	577.5
27	744.0
28	897.5
29	1054.0
30	1232.0
31	1433.5
32	1672.5
33	1873.0
34	1968.5
35	2040.5
36	2129.5
37	2156.0
38	2129.5
39	2121.0
40	2258.5
41	2361.0
42	2294.0
43	2254.5
44	2229.0
45	2140.5
46	2339.0
47	2845.5
48	3639.5
49	4082.5
50	3850.5
51	3384.0
52	2908.5
53	2707.0
54	2468.5
55	2317.5
56	2437.0
57	2890.0
58	3366.0
59	3419.0
60	2941.5
61	2490.0
62	1974.0
63	1287.0
64	1031.5
65	810.0
66	615.0
67	531.0
68	353.0
69	189.5
70	127.0
71	112.0
72	95.0
73	70.0
74	48.0
75	36.0
76	27.5
77	17.0
78	17.0
79	16.5
80	10.0
81	9.5
82	11.0
83	5.5
84	1.0
85	2.0
86	2.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05811178216383371
2	0.04981009899757175
3	0.06018720295539921
4	0.06745117572587843
5	0.07990370047527136
6	0.0830168316626196
7	0.08716767324575057
8	0.09650706680779528
9	0.09443164601622979
10-11	0.09495050121412117
12-13	0.10273332918249176
14-15	0.09702592200568665
16-17	0.0716020173090094
18-19	0.09339393562044705
20-21	0.08976194923520743
22-23	0.058630637361725094
24-25	0.023867339103003135
26-27	0.15513770416952039
28-29	0.062262623746964695
30-31	0.07315858290268352
32-33	0.06693232052798705
34-35	0.02749932548824274
36-37	0.06122491335118195
38-39	0.0773094244858145
40-41	0.08457339725629372
42-43	0.07990370047527136
44-45	0.0773094244858145
46-47	0.08146026606894548
48-49	0.08197912126683686
50-51	0.07679056928792312
52-53	0.07990370047527136
54-55	0.07108316211111802
56-57	0.07263972770479214
58-59	0.07212087250690077
60-61	0.06952659651744392
62-63	0.07108316211111802
64-65	0.0716020173090094
66-67	0.0716020173090094
68-69	0.07315858290268352
70-71	0.06537575493431293
72-73	0.06433804453853019
74-75	0.06070605815329058
76-77	0.06070605815329058
78-79	0.05811178216383371
80-81	0.053442085382811366
82-83	0.05914949255961646
84-85	0.04825353340389764
86-87	0.046696967810223526
88-89	0.04929124379968038
90-91	0.047215823008114895
92-93	0.048772388601789014
94-95	0.046696967810223526
96-97	0.04981009899757175
98-99	0.04981009899757175
100	0.057074071768050974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	96366.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.81391777182823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.02857943962668	43.020359877965255
2	6.607117822595181	6.846813191374551
3	2.8138831587590873	4.37394931822427
4	1.454006529010034	3.013510989353091
5	1.1636057759708396	3.014548699748874
6	0.8031082894394264	2.4967312122532843
7	0.5928180889627687	2.1501359400618476
8	0.4386052752798863	1.818068613411369
9	0.3805251246720474	1.7744847767884937
>10	2.5915763753980494	24.060353236618724
>50	0.10013819070317038	3.3341635016499596
>100	0.02403316576876089	1.9519332544673431
>500	0.0	0.0
>1k	0.0020027638140634072	2.1449473880829335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2067	2.1449473880829335	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	326	0.33829358902517487	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	209	0.2168814727185937	No Hit
GTACGGGAGAGACGTGAGTTCCTGGGTGGGGCACTATGGCGCCCTTGTGA	195	0.20235352717763527	No Hit
CTCCCGTACTGCTCAAAAGATACGTAGCGGATGCCCTTGCCAAAGTTGGT	174	0.18056160886619763	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	163	0.16914679451258743	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	149	0.154618848971629	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130	0.13490235145175686	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	112	0.11622356432766742	No Hit
GTACATGCATCGAGAGGACTAATGGAGCGCTTAGAAGTGAGAATGCCGGT	108	0.11207272274453646	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.1110350123487537	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	105	0.10895959155718822	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	103	0.10688417076562273	No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	100	0.1037710395782745	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.003113131187348235	0.0
20-21	0.0	0.0	0.0	0.004669696781022352	0.0
22-23	0.0	0.0	0.0	0.009858248759936076	0.0
24-25	0.0	0.0	0.0	0.019716497519872152	0.0
26-27	0.0	0.0	0.0	0.05032895419546313	0.0
28-29	0.0	0.0	0.0	0.12348753709814665	0.0
30-31	0.0	0.0	0.0	0.2282962870722039	0.0
32-33	0.0	0.0	0.0	0.3870659776269639	0.0
34-35	0.0	0.0	0.0	0.685407716414503	0.0
36-37	0.0	0.0	0.0	1.1181329514559075	0.0
38-39	0.0	0.0	0.0	1.586659195151817	0.0
40-41	0.0	0.0	0.0	2.021459850984787	0.0
42-43	0.0	0.0	0.0	2.3716871095614636	0.0
44-45	0.0	0.0	0.0	2.681962517900504	0.0
46-47	0.0	0.0	0.0	3.0436045908307907	0.0
48-49	0.0	0.0	0.0	3.3927941390116847	0.0
50-51	0.0	0.0	0.0	3.7409459767967954	0.0
52-53	0.0	0.0	0.0	4.034099163605421	0.0
54-55	0.0	0.0	0.0	4.363053359068552	0.0
56-57	0.0	0.0	0.0	4.751675902289189	0.0
58-59	0.0	0.0	0.0	5.110204844032127	0.0
60-61	0.0	0.0	0.0	5.443828736276281	0.0
62-63	0.0	0.0	0.0	5.7572172758026685	0.0
64-65	0.0	0.0	0.0	6.118340493535064	0.0
66-67	0.0	0.0	0.0	6.513189299130399	0.0
68-69	0.0	0.0	0.0	6.9521407965465	0.0
70-71	0.0	0.0	0.0	7.429487578606563	0.0
72-73	0.0	0.0	0.0	7.916173754228669	0.0
74-75	0.0	0.0	0.0	8.416869020193843	0.0
76-77	0.0	0.0	0.0	8.939356204470457	0.0
78-79	0.0	0.0	0.0	9.557831600356973	0.0
80-81	0.0	0.0	0.0	10.165411037087768	0.0
82-83	0.0	0.0	0.0	10.809829192868854	0.0
84-85	0.0	0.0	0.0	11.468256438993006	0.0
86-87	0.0	0.0	0.0	12.125127119523484	0.0
88	0.0	0.0	0.0	12.644501172612747	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	20	0.0021567033	70.501945	1
CAGAACA	25	0.0052209306	56.401558	4
GCCGGAA	25	0.0052209306	56.401558	94
TCTAGAG	25	0.0052209306	56.401558	2
TACATGA	45	1.6025559E-5	52.223667	2
AGTACAA	55	5.2460622E-5	42.728455	2
ACATGAA	70	4.5901397E-6	40.286827	3
GGAAACA	180	0.0	39.16775	1
GAGAACA	180	0.0	36.55657	1
AGAACAA	155	1.8189894E-12	36.3881	2
CATGGAA	55	0.0027043344	34.182766	2
GTACAAA	140	4.984031E-10	33.572357	1
CATGAAA	75	3.2529148E-4	31.3342	4
ATCAACA	240	0.0	31.3342	2
GTCAAGA	60	0.0041416595	31.3342	1
TGGTACA	30	0.0041518887	31.3342	58-59
TAGACAG	95	3.7163263E-5	29.68503	5
CCCCTTG	40	4.7654918E-4	29.37581	48-49
TCAACAC	225	0.0	29.245255	3
ACAACAC	260	0.0	28.923876	5
>>END_MODULE