FastQCFastQC Report
Wed 25 May 2016
SRR1033093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96366
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17611.827408006973414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17391.8045783782661935No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT14931.549301620903638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.9266753834339913No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT6140.6371541830106053RNA PCR Primer, Index 26 (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.5188551978913725No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT4100.42546126227092546RNA PCR Primer, Index 26 (100% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.36112321773239525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.32895419546313015No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3090.3206525122968682No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.30197372517277876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.25942759894568623No Hit
CTCCCGTACTGCTCAAAAGATACGTAGCGGATGCCCTTGCCAAAGTTGGT2260.23452254944690037No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2220.23037170786376937No Hit
GTACGGGAGAGACGTGAGTTCCTGGGTGGGGCACTATGGCGCCCTTGTGA2040.21169292073968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2030.2106552103438972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1970.20442894796920075No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1730.17952389847041486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1710.1774484776788494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1620.16810908411680467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1530.15876969055475998No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.1432040346180188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1200.12452524749392939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1170.12141211630658116No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.1131104331403192No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1080.11207272274453646No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1080.11207272274453646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1000.1037710395782745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC980.101695618786709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA301.365271E-462.6625149
TCTTAAG250.005158789556.572411
TTAAGGC250.005222903656.3962633
TACGGGA751.4274337E-744.0007632
ACGGGAG751.4629222E-743.8637583
GAGAGAC853.9076622E-738.703327
ATTGAGC500.001694851937.5975078
GTACGGG905.967577E-736.66731
AGAGACG906.1153696E-736.5531358
GGTATCA13200.036.4292031
GAGACGT959.336636E-734.6292849
CCGTACT851.7390179E-533.1742744
CGTACTG851.7390179E-533.1742745
TACTGCT902.5706982E-531.3312577
CGGGAGA1102.9329312E-629.907114
GGGAGAG1303.280311E-728.9211625
GTATCAA18750.028.6633531
TCCCGTA1005.1650553E-528.2862052
GTACTGC1005.2761752E-528.1981326
ACTGCTC1057.355768E-526.8553648
Produced by FastQC (version 0.11.5)