##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033093_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96366 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.419297262519976 33.0 31.0 34.0 31.0 34.0 2 32.38168026067285 34.0 31.0 34.0 30.0 34.0 3 32.391891330967354 34.0 31.0 34.0 30.0 34.0 4 36.04043957412365 37.0 35.0 37.0 35.0 37.0 5 36.059305149118984 37.0 35.0 37.0 35.0 37.0 6 36.18292758856858 37.0 36.0 37.0 35.0 37.0 7 36.11853765851026 37.0 36.0 37.0 35.0 37.0 8 36.152906626818584 37.0 36.0 37.0 35.0 37.0 9 37.88316418653882 39.0 38.0 39.0 35.0 39.0 10-11 37.85322105306851 39.0 38.0 39.0 35.0 39.0 12-13 37.80129921341552 39.0 38.0 39.0 35.0 39.0 14-15 39.04097399497748 40.5 38.0 41.0 36.0 41.0 16-17 39.049073324616565 40.0 38.5 41.0 36.0 41.0 18-19 39.216279600689035 41.0 39.0 41.0 36.0 41.0 20-21 39.23591826992923 41.0 39.0 41.0 36.0 41.0 22-23 39.08337484174916 41.0 39.0 41.0 35.0 41.0 24-25 38.91152481165557 41.0 38.5 41.0 35.0 41.0 26-27 38.608243571384094 40.0 38.0 41.0 35.0 41.0 28-29 38.39187576531142 40.0 38.0 41.0 35.0 41.0 30-31 37.968655957495386 40.0 37.0 41.0 34.0 41.0 32-33 37.35283710022207 40.0 35.0 41.0 33.0 41.0 34-35 36.92425751821182 40.0 35.0 41.0 33.0 41.0 36-37 36.5094483531536 40.0 35.0 41.0 32.5 41.0 38-39 36.0608513376087 40.0 35.0 41.0 30.5 41.0 40-41 35.664637942842916 39.5 35.0 41.0 30.0 41.0 42-43 35.05829857003508 39.0 35.0 41.0 24.5 41.0 44-45 34.19315422451902 39.0 35.0 41.0 8.5 41.0 46-47 32.95119129153436 39.0 34.5 41.0 2.0 41.0 48-49 31.772082477222256 39.0 33.0 41.0 2.0 41.0 50-51 31.077926862171303 39.0 31.5 41.0 2.0 41.0 52-53 30.7882136853247 38.0 31.0 41.0 2.0 41.0 54-55 30.515301039785818 38.0 31.0 41.0 2.0 41.0 56-57 30.278194591453417 37.0 30.0 40.0 2.0 41.0 58-59 30.002122117759377 37.0 29.5 40.0 2.0 41.0 60-61 29.617811261233214 36.0 28.5 40.0 2.0 41.0 62-63 29.26366664591246 35.0 28.0 39.0 2.0 41.0 64-65 28.87628935516676 35.0 27.5 39.0 2.0 41.0 66-67 28.33911338023784 35.0 26.0 38.5 2.0 41.0 68-69 27.75276549820476 35.0 22.0 37.0 2.0 40.0 70-71 27.13244816636573 35.0 13.0 37.0 2.0 39.5 72-73 26.732120249880666 35.0 4.5 36.0 2.0 39.0 74-75 26.482358923271693 35.0 2.0 36.0 2.0 39.0 76-77 26.21038540564099 35.0 2.0 35.0 2.0 37.5 78-79 25.952872382375524 35.0 2.0 35.0 2.0 37.0 80-81 25.720601664487475 34.5 2.0 35.0 2.0 36.5 82-83 25.544699375298343 34.0 2.0 35.0 2.0 36.0 84-85 25.447756470124318 34.0 2.0 35.0 2.0 36.0 86-87 25.349334827636305 34.0 2.0 35.0 2.0 35.5 88-89 25.238279061079634 34.0 2.0 35.0 2.0 35.0 90-91 25.107340763339767 34.0 2.0 35.0 2.0 35.0 92-93 24.967945125874273 34.0 2.0 35.0 2.0 35.0 94-95 24.834474814768694 34.0 2.0 35.0 2.0 35.0 96-97 24.703173318390306 34.0 2.0 35.0 2.0 35.0 98-99 24.574139219226698 34.0 2.0 35.0 2.0 35.0 100 24.471743145922837 34.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5517433295140322 1101 2 0.34298837053749764 1101 3 0.49920747148042466 1101 4 0.08151517147258147 1101 5 0.24095880895942656 1101 6 0.20716254449529004 1101 7 0.34135087788055074 1101 8 0.31847941201412766 1101 9 0.4765279283895367 1101 10-11 0.33117267858946065 1101 12-13 0.42321386145946605 1101 14-15 0.4416890278844008 1101 16-17 0.39398633480114853 1101 18-19 0.47399008195404235 1101 20-21 0.4649748833131042 1101 22-23 0.6221654428027037 1101 24-25 0.706897990768951 1101 26-27 0.5852062803089311 1101 28-29 0.994110583755301 1101 30-31 0.6842887396960151 1101 32-33 1.078893131439031 1101 34-35 1.4710265441069055 1101 36-37 1.5280209599723733 1101 38-39 1.339384426385699 1101 40-41 1.352487913871137 1101 42-43 1.7212571565991581 1101 44-45 2.400571543931264 1101 46-47 2.1412907054764787 1101 48-49 2.3182833268542886 1101 50-51 2.203339200367431 1101 52-53 1.968156032761474 1101 54-55 2.1012732080703387 1101 56-57 2.0799838394209083 1101 58-59 2.0500226472726055 1101 60-61 1.9502862635722131 1101 62-63 2.2218129268624303 1101 64-65 2.3071758929713333 1101 66-67 2.046805102168925 1101 68-69 2.331312458922568 1101 70-71 2.8278276145730175 1101 72-73 2.674707532379376 1101 74-75 2.126936300949673 1101 76-77 1.6439292084911123 1101 78-79 1.8426304983296227 1101 80-81 2.049033032320324 1101 82-83 1.9791688437147599 1101 84-85 2.1215396403379394 1101 86-87 1.9379624268479674 1101 88-89 1.9723197107500674 1101 90-91 2.011936781212576 1101 92-93 2.2977901944608163 1101 94-95 2.146375369320218 1101 96-97 2.723818603875838 1101 98-99 2.223921558968698 1101 100 2.247700883864251 1102 1 0.16136504556590126 1102 2 0.3153057796619976 1102 3 0.0861859032482073 1102 4 0.19318620553828936 1102 5 0.2355835485952582 1102 6 0.24109387554413786 1102 7 0.08125546700908615 1102 8 -0.014652349055545244 1102 9 0.3153373082188722 1102 10-11 -0.15179446513156591 1102 12-13 0.04506429483983254 1102 14-15 0.32733036363660517 1102 16-17 0.3905932016962623 1102 18-19 -0.13959588861640526 1102 20-21 -0.09989779020702372 1102 22-23 -0.0888471218683975 1102 24-25 -0.1539835519307715 1102 26-27 0.03625781561767383 1102 28-29 0.011244226166105875 1102 30-31 -0.18573074562280567 1102 32-33 -0.21819078981340567 1102 34-35 -0.28866572223724773 1102 36-37 -0.37324222621320047 1102 38-39 -0.5429309670161686 1102 40-41 -0.7640208545223359 1102 42-43 -0.8877598426617439 1102 44-45 -0.505831734977555 1102 46-47 -0.6593543257672252 1102 48-49 -0.6449633304056732 1102 50-51 -0.9700465417544493 1102 52-53 -0.6151269075059425 1102 54-55 -0.8411779106557304 1102 56-57 -0.43872878572187446 1102 58-59 -0.49657414100932584 1102 60-61 -0.44976748903143005 1102 62-63 -0.48217820395796807 1102 64-65 -0.6606733309754524 1102 66-67 -1.1750251739850768 1102 68-69 -1.171173598995864 1102 70-71 -1.6411302568238781 1102 72-73 -1.4393823688502145 1102 74-75 -1.221750119798834 1102 76-77 -0.8793859833384943 1102 78-79 -0.6149721355479549 1102 80-81 -0.8874381092505956 1102 82-83 -0.8393825236170969 1102 84-85 -0.5150927590439061 1102 86-87 -0.808392474717575 1102 88-89 -0.9396939961638573 1102 90-91 -1.1054454669900693 1102 92-93 -1.240575726388478 1102 94-95 -0.7654703607073294 1102 96-97 -0.4921660766321203 1102 98-99 -0.5546849047818903 1102 100 -0.797988829231226 1103 1 0.2851455333707804 1103 2 0.18217569836118486 1103 3 0.3983810251994271 1103 4 0.30761709984723495 1103 5 0.27704696322940237 1103 6 0.20227273733275553 1103 7 -0.1378502240478241 1103 8 0.17254277289567455 1103 9 0.4966381212270008 1103 10-11 0.5036933397464836 1103 12-13 0.6461821810187018 1103 14-15 0.8877978433114038 1103 16-17 0.5894753155174044 1103 18-19 0.5592862252047368 1103 20-21 0.4836387951588321 1103 22-23 0.7032260488633142 1103 24-25 0.6668497814025613 1103 26-27 0.37904236846320316 1103 28-29 0.48532959201976666 1103 30-31 0.7322570592552324 1103 32-33 0.8373986410809096 1103 34-35 0.8624521639416116 1103 36-37 0.7711683428924871 1103 38-39 0.876987732170825 1103 40-41 0.6913653243394577 1103 42-43 -0.23328829794629513 1103 44-45 -0.4848967756279592 1103 46-47 -0.5358787160111262 1103 48-49 0.23278057203335223 1103 50-51 0.2942827265382313 1103 52-53 0.36292187298186107 1103 54-55 0.4113627397507784 1103 56-57 0.3499907264732478 1103 58-59 0.36348683460042963 1103 60-61 0.23031381178157773 1103 62-63 0.5680250480745492 1103 64-65 0.44694862024406135 1103 66-67 0.18757645203118045 1103 68-69 0.22841989693909426 1103 70-71 -0.18391480966941032 1103 72-73 -0.2751547265737919 1103 74-75 -0.2506119084167189 1103 76-77 0.17254491097044777 1103 78-79 -0.10826481847478675 1103 80-81 0.04355782570875277 1103 82-83 0.06742641947233352 1103 84-85 -0.056352921645530074 1103 86-87 -0.07536401943302451 1103 88-89 -0.13318993112320854 1103 90-91 -0.18207148325023326 1103 92-93 -0.5325472711039261 1103 94-95 -0.39463702737399586 1103 96-97 -0.23007258069716485 1103 98-99 -0.17287596169245845 1103 100 -0.3038831381743172 1104 1 -0.09993198600906794 1104 2 -0.08042120086361848 1104 3 0.024737614346719283 1104 4 -0.027460419532609137 1104 5 -0.11287551739075496 1104 6 -0.06652571227964899 1104 7 -0.08474944885402635 1104 8 -0.1303642038485151 1104 9 0.0012892840177016751 1104 10-11 0.2643522544751633 1104 12-13 0.17864342132877198 1104 14-15 -0.13788045126224802 1104 16-17 0.05933965660267404 1104 18-19 -0.04836881355495848 1104 20-21 -0.1545394994148097 1104 22-23 -0.023711935632817926 1104 24-25 0.1948536573715529 1104 26-27 -0.03946538347478423 1104 28-29 -0.0839533537166659 1104 30-31 -0.16880883221763554 1104 32-33 0.10474360232122137 1104 34-35 -0.011675743035127084 1104 36-37 0.4859939242878326 1104 38-39 0.028538119767723913 1104 40-41 0.5011521460448805 1104 42-43 0.3597349578676585 1104 44-45 0.3893280305735942 1104 46-47 0.1613112064694917 1104 48-49 0.8111720449015642 1104 50-51 0.5459300133599356 1104 52-53 0.708658307090392 1104 54-55 0.355645685487211 1104 56-57 0.26830467996162 1104 58-59 0.2599015632825967 1104 60-61 0.6415541218590981 1104 62-63 0.005815745748968482 1104 64-65 0.25925482179444614 1104 66-67 0.33253769234126196 1104 68-69 0.3799702845359967 1104 70-71 -0.18556209649111466 1104 72-73 0.21631814164326357 1104 74-75 0.5528764636763057 1104 76-77 0.3728356086448663 1104 78-79 0.15161890245544285 1104 80-81 0.3068330195072022 1104 82-83 0.4386473497048904 1104 84-85 0.6779494039358624 1104 86-87 0.7774460580863582 1104 88-89 0.7187480533729165 1104 90-91 0.9518432454319381 1104 92-93 0.4886736591286329 1104 94-95 0.773289329215153 1104 96-97 0.5822336208532235 1104 98-99 0.45202713908273395 1104 100 0.22848120291095597 1105 1 0.2807051440763786 1105 2 -0.2646004573565719 1105 3 0.1093299303089168 1105 4 0.14636405848470702 1105 5 0.1585554790444874 1105 6 -0.00898030402976957 1105 7 0.22504515308113326 1105 8 0.32722647119251036 1105 9 -0.07538134349319137 1105 10-11 0.24548166091437196 1105 12-13 0.4078914267607914 1105 14-15 0.25899614014838335 1105 16-17 0.3988122984857725 1105 18-19 0.566920045156067 1105 20-21 0.7107069216795168 1105 22-23 0.6138708177197572 1105 24-25 0.7462597570716554 1105 26-27 0.755532636103105 1105 28-29 0.8653478401949428 1105 30-31 0.8696962320046282 1105 32-33 1.2808597116405167 1105 34-35 1.2408280763503754 1105 36-37 1.2408459584642557 1105 38-39 1.0094694839956517 1105 40-41 1.6908482927092834 1105 42-43 1.6937190013237782 1105 44-45 0.9122443435934571 1105 46-47 0.9052709190253694 1105 48-49 0.6735044163155841 1105 50-51 0.8302620452730238 1105 52-53 0.910123819453883 1105 54-55 0.9051279465634252 1105 56-57 0.8798873201472155 1105 58-59 0.7481887810724501 1105 60-61 0.9382213543606568 1105 62-63 0.8551904981962046 1105 64-65 1.0052515399520878 1105 66-67 1.263732169792739 1105 68-69 1.4229454443843537 1105 70-71 0.8427579397223184 1105 72-73 0.42825159946027114 1105 74-75 0.21739295776333023 1105 76-77 0.12610330513103207 1105 78-79 0.3624772739826341 1105 80-81 0.1841600154897769 1105 82-83 0.25322325645529986 1105 84-85 0.4139025528070235 1105 86-87 0.12107758640639332 1105 88-89 0.3597541078062285 1105 90-91 0.4316146481366303 1105 92-93 0.36894299653597784 1105 94-95 0.03136662226103937 1105 96-97 0.18346148256317818 1105 98-99 0.5586021404973209 1105 100 0.3939879080543953 1106 1 -0.0026204240760279163 1106 2 0.14405513098530065 1106 3 -0.10374663437503528 1106 4 0.020205752329502502 1106 5 0.009671076704577786 1106 6 0.2052869217299218 1106 7 -0.04104171340952689 1106 8 0.29896121261198516 1106 9 -0.043078190829731966 1106 10-11 0.14642383620038402 1106 12-13 0.1386467200257897 1106 14-15 0.25260280785040834 1106 16-17 0.18708169849612943 1106 18-19 0.22781459399905657 1106 20-21 0.12246858239287661 1106 22-23 -0.05191579510832156 1106 24-25 -0.21426723987403307 1106 26-27 -0.15030160316800334 1106 28-29 -0.4238016136539926 1106 30-31 -0.5009876215958258 1106 32-33 -0.4524063234580993 1106 34-35 -0.08870031832789493 1106 36-37 -0.2595940684550371 1106 38-39 -0.3786860266944174 1106 40-41 -0.11776588133430721 1106 42-43 0.07876843964235292 1106 44-45 0.3837202456486324 1106 46-47 0.9441744754782349 1106 48-49 1.209819579125547 1106 50-51 1.6553642868219214 1106 52-53 1.9898722275917926 1106 54-55 2.0004584844316327 1106 56-57 1.5692283151257271 1106 58-59 1.5158804516217046 1106 60-61 1.2866967905049833 1106 62-63 0.9423158282163939 1106 64-65 1.0982784074781016 1106 66-67 0.9946686506127449 1106 68-69 1.4514695423291677 1106 70-71 1.3787624952951276 1106 72-73 1.2149161954120231 1106 74-75 1.0461966022215776 1106 76-77 0.74195980458747 1106 78-79 0.7231181602486174 1106 80-81 0.8230791023044972 1106 82-83 0.9237207457134673 1106 84-85 0.8693562982126259 1106 86-87 0.9449373990066938 1106 88-89 1.129930636252677 1106 90-91 1.0978930557568631 1106 92-93 1.1758747147116786 1106 94-95 0.922472901704019 1106 96-97 0.8113281285226925 1106 98-99 0.8325318397259807 1106 100 0.8704076419675282 1107 1 0.057177496841099185 1107 2 -0.04442247515479636 1107 3 0.09153171013093697 1107 4 0.0383248624043091 1107 5 0.14325700889150284 1107 6 0.15695310262955786 1107 7 0.18109954764167213 1107 8 0.19388980485913976 1107 9 0.11124999337312147 1107 10-11 0.17663854522593425 1107 12-13 0.30742647325614314 1107 14-15 0.32986405335705626 1107 16-17 0.038048374878130176 1107 18-19 0.06379535762481936 1107 20-21 -0.08976303132519092 1107 22-23 -0.07428536666180463 1107 24-25 -0.37430318663396633 1107 26-27 -0.28813799683360486 1107 28-29 -0.32203342167886717 1107 30-31 0.04452874875295265 1107 32-33 -0.6540967927090477 1107 34-35 -1.003472493592632 1107 36-37 -1.019528004139481 1107 38-39 -1.265478021253827 1107 40-41 -1.2203355889025502 1107 42-43 -0.6065759691791683 1107 44-45 -0.9322712048517019 1107 46-47 -0.5298855653261967 1107 48-49 -0.37344088915386564 1107 50-51 0.2832667448030719 1107 52-53 -0.016758492314941975 1107 54-55 0.10628283107497793 1107 56-57 0.14491081779744874 1107 58-59 0.2665119487556815 1107 60-61 0.39492568392769556 1107 62-63 0.40238577866815817 1107 64-65 0.7467773873673487 1107 66-67 0.34944859814990537 1107 68-69 0.4284198969390971 1107 70-71 0.21294592092419862 1107 72-73 0.1992744971704994 1107 74-75 0.15874882217688935 1107 76-77 -0.21461262327612474 1107 78-79 0.09732878883114893 1107 80-81 0.0177016613251908 1107 82-83 0.17593098568237764 1107 84-85 0.09878406465583822 1107 86-87 -0.09985032080288647 1107 88-89 -0.04460545623736678 1107 90-91 -0.03926326407214731 1107 92-93 0.07869702113351806 1107 94-95 0.16032872605066117 1107 96-97 0.6222676019512399 1107 98-99 0.3327747232390479 1107 100 0.34874243260193794 1108 1 -0.4045730813478343 1108 2 0.03661292779841574 1108 3 -0.0574631306447273 1108 4 -0.0052616880315525805 1108 5 0.06622445240689245 1108 6 -0.09567098561096543 1108 7 -0.10256883876643741 1108 8 -0.22107194571904643 1108 9 -0.23539651080762525 1108 10-11 -0.21221575116260993 1108 12-13 -0.05701045967394691 1108 14-15 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-1.0165128165278006 2215 38-39 -0.8442079200030861 2215 40-41 -0.9432361249359076 2215 42-43 -1.0485056892506393 2215 44-45 -1.133990978526512 2215 46-47 -0.6146830638372123 2215 48-49 -0.20671218158983962 2215 50-51 0.08631171204547883 2215 52-53 -0.1836360980326326 2215 54-55 0.12965984120005203 2215 56-57 -0.06540782425139113 2215 58-59 -0.3183972233705852 2215 60-61 -0.36769343459523185 2215 62-63 -0.22038074902689786 2215 64-65 -0.3487035536689831 2215 66-67 -0.4680394899978033 2215 68-69 -0.5308192334956843 2215 70-71 -0.16253799807520863 2215 72-73 0.2858959980638893 2215 74-75 0.47891707709052866 2215 76-77 0.2100449109704492 2215 78-79 0.01256851485854682 2215 80-81 0.1951882604913635 2215 82-83 0.20347714411001405 2215 84-85 0.5311470783544685 2215 86-87 0.5018098936104529 2215 88-89 0.3997810833695432 2215 90-91 0.4369502558802054 2215 92-93 0.2426338883163659 2215 94-95 0.3423194943651353 2215 96-97 0.13641292654921244 2215 98-99 0.12766751656840825 2215 100 0.39974005023147896 2216 1 -0.20011087688563123 2216 2 -0.14410635292086482 2216 3 -0.05290102608262259 2216 4 -0.2199470027168715 2216 5 -0.33577354959111005 2216 6 -0.32913751907749855 2216 7 -0.5032348394324373 2216 8 -0.3383546630017662 2216 9 -0.7059259813370957 2216 10-11 -0.6174605064073617 2216 12-13 -0.39965115231463244 2216 14-15 -0.7477790797655217 2216 16-17 -0.4082810947390101 2216 18-19 -0.5923163388978381 2216 20-21 -0.4336688971488627 2216 22-23 -0.5845944639572025 2216 24-25 -0.5180861160333379 2216 26-27 -0.898201221280388 2216 28-29 -0.8137088695186918 2216 30-31 -0.4693455048473254 2216 32-33 0.16592428210654475 2216 34-35 0.030721394710845118 2216 36-37 -0.31633165710751143 2216 38-39 -0.47814047295737794 2216 40-41 -0.7794680089938808 2216 42-43 -0.4794421441001404 2216 44-45 -0.20733267306385983 2216 46-47 -0.32914794678035264 2216 48-49 -0.011129684376907534 2216 50-51 0.010628880384384587 2216 52-53 0.5321526422126333 2216 54-55 0.6802729961610297 2216 56-57 0.6330035469860675 2216 58-59 0.816371449985045 2216 60-61 0.21877535024311712 2216 62-63 0.009691714741215662 2216 64-65 0.016499902295340263 2216 66-67 0.008089272544001602 2216 68-69 0.01944553796473869 2216 70-71 0.07538006212546122 2216 72-73 0.41715296573389793 2216 74-75 0.3199009120961023 2216 76-77 0.4254295263550638 2216 78-79 0.06288902767905924 2216 80-81 0.06759628724721622 2216 82-83 0.36774693229284594 2216 84-85 0.2635188732262641 2216 86-87 0.355084698515693 2216 88-89 0.18475878682550828 2216 90-91 0.05446697828822877 2216 92-93 0.3434142673576126 2216 94-95 0.12683733160036326 2216 96-97 -0.18359822172280715 2216 98-99 -0.478324679641176 2216 100 -0.4801651894563683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 12.0 6 32.0 7 87.0 8 128.0 9 191.0 10 346.0 11 549.0 12 957.0 13 1324.0 14 1355.0 15 1880.0 16 2645.0 17 3686.0 18 4295.0 19 1804.0 20 658.0 21 576.0 22 541.0 23 592.0 24 721.0 25 1050.0 26 1292.0 27 1493.0 28 946.0 29 974.0 30 1116.0 31 1419.0 32 1708.0 33 2145.0 34 3055.0 35 5033.0 36 9225.0 37 19390.0 38 20334.0 39 4793.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.13149643902743 17.865077552376405 14.164988268516018 20.83843774008015 2 17.834785683089656 21.062537695235324 38.022793918848656 23.079882702826364 3 20.201527541405504 26.263957494500023 32.02855838279856 21.50595658129592 4 12.78147894485607 19.958284042089534 32.97117240520516 34.28906460784924 5 11.82159682875703 36.660232862212816 33.272108419982146 18.246061889048004 6 28.50901770333935 36.536745325114666 14.893219600273955 20.061017371272026 7 22.35124421476454 37.22370960712284 19.83583421538717 20.589211962725443 8 25.61614694131687 31.077673429149588 19.93047268199035 23.375706947543197 9 25.486167320424215 14.747940144864371 20.407612643463462 39.358279891247946 10-11 24.49009240859021 25.937187063835086 25.282388406639306 24.290332120935396 12-13 27.828798538903765 24.542888570657702 26.91976423219808 20.70854865824046 14-15 24.36440238258307 23.496357636510805 29.625075234003695 22.514164746902434 16-17 18.94236556461823 27.348338625656353 30.34576510387481 23.36353070585061 18-19 19.115243525950678 21.50873497257836 38.48005769699737 20.895963804473595 20-21 19.7497042525372 19.65475375132308 39.979868418321814 20.6156735778179 22-23 18.940907274906476 20.4896980765938 40.7718529971826 19.797541651317122 24-25 20.482540341410264 21.188709593732163 40.04981061588751 18.27893944897006 26-27 19.172737272481996 20.30539816947886 41.42124815806405 19.100616399975095 28-29 18.555914720517197 19.30255122424519 42.595119622686546 19.546414432551067 30-31 19.675507959238736 19.981113670796756 40.39858456302015 19.944793806944357 32-33 19.69211132557126 20.13832679575784 41.886142415374714 18.283419463296184 34-35 19.18570865242928 20.442375941722187 40.55942967436648 19.812485731482056 36-37 18.741568603034263 20.19851399871324 40.75296266317996 20.306954735072537 38-39 19.474192142456882 18.744162879023722 41.23446028682316 20.54718469169624 40-41 18.772181059709855 19.369383392482824 40.747774111201046 21.11066143660627 42-43 19.88512545918685 19.94635037253803 39.56063341842558 20.60789074984953 44-45 19.962953738870556 20.13780794055995 39.040740510138434 20.858497810431064 46-47 20.824253367370236 19.98267023639043 36.668534545379075 22.52454185086026 48-49 21.944461739617708 20.28931365834423 34.094493908639976 23.671730693398086 50-51 20.682087043147998 20.796754041881993 34.10020131581678 24.42095759915323 52-53 20.912458751011766 21.376315297926656 33.692381130274164 24.018844820787415 54-55 20.423178299400202 22.025403150488764 32.42014818504452 25.131270365066516 56-57 21.183820019508957 22.084033787850487 30.922213228732126 25.809932963908434 58-59 19.709752402299564 22.753356993130357 31.777286594857106 25.759604009712973 60-61 20.964863125998797 23.851773447066392 29.887615964136728 25.295747462798087 62-63 19.600273955544488 24.690762302056743 30.60882468920574 25.100139053193033 64-65 19.016043002718803 25.14683602100326 30.521138160762096 25.31598281551585 66-67 19.69159247037337 24.499304734034826 29.797335159703632 26.011767635888177 68-69 19.878899196812153 24.62538654712243 29.98619845173609 25.50951580432933 70-71 19.80262748272212 24.48685220928543 29.235933835585165 26.47458647240728 72-73 20.39567897391196 23.30593777888467 29.9856795965382 26.31270365066517 74-75 20.077620737604548 23.16273374426665 29.553992071892576 27.205653446236223 76-77 20.234833862565633 22.934956312392337 29.41545773405558 27.41475209098645 78-79 21.104435174231575 22.25992569993566 29.5944627773281 27.04117634850466 80-81 20.744349666894962 22.29624556378806 29.38588298777577 27.573521781541206 82-83 20.61826785380736 21.413154016976943 29.98671730693398 27.981860822281718 84-85 21.139198472490296 22.21997384969803 28.826038229250983 27.814789448560695 86-87 20.960193429217775 22.997737791337194 27.977191125500696 28.064877653944336 88-89 20.27478571280327 22.29832098457962 28.144262499221718 29.28263080339539 90-91 20.713218355021482 22.252661727165183 28.078367889089513 28.955752028723825 92-93 21.06448332399394 21.931490359670423 28.50331029616255 28.50071602017309 94-95 20.5798725691634 21.8007388498018 28.34557831600357 29.273810265031237 96-97 20.578316003569725 21.786210904260837 27.85370358840255 29.781769503766885 98-99 20.340680322935476 21.951725712388185 27.346263204864783 30.36133075981155 100 21.059294772015026 21.272025403150487 27.217068260589834 30.45161156424465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 192.0 1 117.5 2 126.5 3 217.5 4 132.0 5 64.5 6 164.5 7 290.5 8 400.5 9 451.5 10 305.5 11 143.5 12 109.5 13 94.5 14 96.5 15 110.0 16 110.0 17 113.0 18 143.5 19 149.5 20 160.0 21 209.0 22 277.0 23 325.0 24 389.0 25 500.5 26 609.0 27 744.5 28 923.0 29 1091.5 30 1293.5 31 1495.5 32 1755.5 33 2062.5 34 2214.5 35 2224.5 36 2269.5 37 2321.5 38 2185.5 39 2029.0 40 2132.5 41 2270.0 42 2237.5 43 2242.5 44 2345.0 45 2377.5 46 2538.5 47 3000.5 48 3876.5 49 4267.5 50 3870.0 51 3561.5 52 3046.0 53 2473.0 54 2142.5 55 2084.5 56 2308.0 57 2800.5 58 3277.5 59 3340.0 60 2844.5 61 2383.5 62 1936.0 63 1277.5 64 961.5 65 736.0 66 520.5 67 395.5 68 240.0 69 109.0 70 59.0 71 47.5 72 49.0 73 35.0 74 17.0 75 10.0 76 8.5 77 6.0 78 4.5 79 4.0 80 5.0 81 5.0 82 2.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045659257414440775 2 0.207542079156549 3 0.00207542079156549 4 0.0 5 0.0 6 0.0 7 0.0 8 0.001037710395782745 9 0.0 10-11 5.188551978913725E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.188551978913725E-4 20-21 0.0 22-23 5.188551978913725E-4 24-25 0.001037710395782745 26-27 0.0 28-29 5.188551978913725E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 96366.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.441649544445134 #Duplication Level Percentage of deduplicated Percentage of total 1 77.46074104485271 31.326401427889504 2 8.875602997023504 7.1788805180250295 3 3.425536282459202 4.156030135109893 4 2.0347942112285744 3.2916173754228675 5 1.4446269116288617 2.921154764128427 6 1.0417735810325361 2.5278625241267667 7 0.8493277224674125 2.40437498702862 8 0.6543159191214205 2.1169292073967996 9 0.5080570666119265 1.8491999252848514 >10 3.464025454172226 25.785027914409646 >50 0.17191830031817715 4.679036174584398 >100 0.056450785179102944 5.0183674740053545 >500 0.005131889561736632 1.5638295664445967 >1k 0.007697834342604948 5.181288006143245 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1761 1.827408006973414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1739 1.8045783782661935 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1493 1.549301620903638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 893 0.9266753834339913 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 614 0.6371541830106053 RNA PCR Primer, Index 26 (100% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 500 0.5188551978913725 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 410 0.42546126227092546 RNA PCR Primer, Index 26 (100% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 348 0.36112321773239525 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317 0.32895419546313015 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 309 0.3206525122968682 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.30197372517277876 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250 0.25942759894568623 No Hit CTCCCGTACTGCTCAAAAGATACGTAGCGGATGCCCTTGCCAAAGTTGGT 226 0.23452254944690037 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 222 0.23037170786376937 No Hit GTACGGGAGAGACGTGAGTTCCTGGGTGGGGCACTATGGCGCCCTTGTGA 204 0.21169292073968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 203 0.2106552103438972 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 197 0.20442894796920075 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 173 0.17952389847041486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 171 0.1774484776788494 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 162 0.16810908411680467 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 153 0.15876969055475998 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.1432040346180188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 120 0.12452524749392939 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 117 0.12141211630658116 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.1131104331403192 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 108 0.11207272274453646 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 108 0.11207272274453646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 100 0.1037710395782745 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 98 0.101695618786709 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.003113131187348235 0.0 20-21 0.0 0.0 0.0 0.004669696781022352 0.0 22-23 0.0 0.0 0.0 0.009858248759936076 0.0 24-25 0.0 0.0 0.0 0.019716497519872152 0.0 26-27 0.0 0.0 0.0 0.05032895419546313 0.0 28-29 0.0 0.0 0.0 0.12400639229603802 0.0 30-31 0.0 0.0 0.0 0.2293339974679866 0.0 32-33 0.0 0.0 0.0 0.38862254322063794 0.0 34-35 0.0 0.0 0.0 0.6885208476018513 0.0 36-37 0.0 0.0 0.0 1.124359213830604 0.0 38-39 0.0 0.0 0.0 1.6001494302969927 0.0 40-41 0.0 0.0 0.0 2.03754436211942 0.0 42-43 0.0 0.0 0.0 2.3919224622792274 0.0 44-45 0.0 0.0 0.0 2.6985658842330285 0.0 46-47 0.0 0.0 0.0 3.066434219538011 0.0 48-49 0.0 0.0 0.0 3.4218500300936014 0.0 50-51 0.0 0.0 0.0 3.7871240894091276 0.0 52-53 0.0 0.0 0.0 4.089616669779797 0.0 54-55 0.0 0.0 0.0 4.432579955585995 0.0 56-57 0.0 0.0 0.0 4.825353340389764 0.0 58-59 0.0 0.0 0.0 5.189589689309507 0.0 60-61 0.0 0.0 0.0 5.532034119917814 0.0 62-63 0.0 0.0 0.0 5.851130066621008 0.0 64-65 0.0 0.0 0.0 6.2169229811344255 0.0 66-67 0.0 0.0 0.0 6.621111180291804 0.0 68-69 0.0 0.0 0.0 7.066288940082602 0.0 70-71 0.0 0.0 0.0 7.558682522881514 0.0 72-73 0.0 0.0 0.0 8.049000684888862 0.0 74-75 0.0 0.0 0.0 8.56214847560343 0.0 76-77 0.0 0.0 0.0 9.106427578191479 0.0 78-79 0.0 0.0 0.0 9.733204657244256 0.0 80-81 0.0 0.0 0.0 10.348566921943423 0.0 82-83 0.0 0.0 0.0 11.0054376024739 0.0 84-85 0.0 0.0 0.0 11.669053400576967 0.0 86-87 0.0 0.0 0.0 12.327480646701119 0.0 88 0.0 0.0 0.0 12.854118672560862 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGTTA 30 1.365271E-4 62.662514 9 TCTTAAG 25 0.0051587895 56.57241 1 TTAAGGC 25 0.0052229036 56.396263 3 TACGGGA 75 1.4274337E-7 44.000763 2 ACGGGAG 75 1.4629222E-7 43.863758 3 GAGAGAC 85 3.9076622E-7 38.70332 7 ATTGAGC 50 0.0016948519 37.597507 8 GTACGGG 90 5.967577E-7 36.6673 1 AGAGACG 90 6.1153696E-7 36.553135 8 GGTATCA 1320 0.0 36.429203 1 GAGACGT 95 9.336636E-7 34.629284 9 CCGTACT 85 1.7390179E-5 33.174274 4 CGTACTG 85 1.7390179E-5 33.174274 5 TACTGCT 90 2.5706982E-5 31.331257 7 CGGGAGA 110 2.9329312E-6 29.90711 4 GGGAGAG 130 3.280311E-7 28.921162 5 GTATCAA 1875 0.0 28.663353 1 TCCCGTA 100 5.1650553E-5 28.286205 2 GTACTGC 100 5.2761752E-5 28.198132 6 ACTGCTC 105 7.355768E-5 26.855364 8 >>END_MODULE