FastQCFastQC Report
Wed 25 May 2016
SRR1033092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618884
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56600.037.2948461
TCAACGC56700.035.3170364
GGTATCA34800.034.7194481
ATCAACG58350.034.3183563
CAACGCA61650.032.557615
AACGCAG65550.030.8330046
TATCAAC73500.027.7562082
GTACATG131100.026.931231
TACATGG131500.025.5586592
ACGCAGA80100.024.5870447
ACATGGG138300.023.5542243
CGCAGAG82450.023.450028
CATGGGG105950.020.0093674
GCAGAGT100200.018.264869
TACACCG6950.018.261515
GAGTACA98650.017.680551
GTATTAG21500.017.0559161
AGTACAT100700.016.1978632
AGAGTAC84300.015.82761410-11
GTATATA33050.015.6473261
Produced by FastQC (version 0.11.5)