FastQCFastQC Report
Wed 25 May 2016
SRR1033092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618884
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.1499929420180286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55240.11959598898781611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.052.682181
GTATCAA110600.046.520151
TCAACGC135650.037.3499374
ATCAACG136400.037.1790243
CAACGCA139150.036.2757725
TATCAAC146200.034.856882
AACGCAG145650.034.753686
ACGCAGA168400.029.7795357
CGCAGAG172050.029.011188
GTACATG134900.026.6143971
GCAGAGT192350.025.631519
TACATGG136850.025.4336282
ACATGGG143000.023.5653573
AGAGTAC176550.021.61607710-11
GTGGTAT28450.020.5358661
CAGAGTA184850.020.37852110-11
CATGGGG107100.020.318074
GAGTACT129400.019.95768712-13
AGTACTT133300.019.56769212-13
GTACTTT140400.018.2101414-15
Produced by FastQC (version 0.11.5)